期刊
ORGANOGENESIS
卷 5, 期 4, 页码 211-216出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/org.5.4.10426
关键词
blood circulation; conceptus culture; ex vivo; ex utero; heartbeat; limb bud; live imaging; milieu interieur; optical tomography; organogenesis
资金
- CRG
- ICREA
- Spanish Ministry of Science (MEC)
- European Community
- MRC [MC_U127574434] Funding Source: UKRI
- Medical Research Council [MC_U127574434] Funding Source: researchfish
Optical projection tomography (OPT) is a technology ideally suited for imaging embryonic organs. We emphasize here recent successes in translating this potential into the field of live imaging. Live OPT (also known as 4D OPT, or time-lapse OPT) is already in position to accumulate good quantitative data on the developmental dynamics of organogenesis, a prerequisite for building realistic computer models and tackling new biological problems. Yet, live OPT is being further developed by merging state-of-the-art mouse embryo culture with the OPT system. We discuss the technological challenges that this entails and the prospects for expansion of this molecular imaging technique into a wider range of applications.
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