期刊
ORAL MICROBIOLOGY AND IMMUNOLOGY
卷 24, 期 2, 页码 104-108出版社
WILEY
DOI: 10.1111/j.1399-302X.2008.00480.x
关键词
biofilm formation; recombinase A; Streptococcus mutans
资金
- Japan Society for the Promotion of Science [18592233]
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Grants-in-Aid for Scientific Research [18592233] Funding Source: KAKEN
Recombinase A (RecA) is essential for the transformation of both plasmid and chromosomal DNA in Streptococcus pneumoniae and is considered to be related to the SOS-response in Streptococcus mutans. In the present study, a RecA-deficient mutant strain (RAD) was constructed by insertional inactivation of the recA gene encoding the RecA protein in strain MT8148 of S. mutans, after which the biological functions of acid tolerance and biofilm formation were investigated. RAD showed reduced acid tolerance and produced lower density biofilm compared with the wild-type strain. In addition, confocal microscopic observation indicated that the biofilm produced by RAD was composed of cells with significantly lower viability compared with that produced by strain MT8148. These results suggest that RecA has a relationship with biofilm formation.
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