Article
Materials Science, Multidisciplinary
Xiao Yang, Feng Lou, Weihao Yuan, Wanyin Song, Yiyuan Xue, Duan Wang, Yuchen Guo, Xu Peng, Xinyuan Xu, Chuang Liu, Ruibo Zhao, Xiao Yangh, Xianlong Wang, Chunmei Ding, Liming Bian, Jianshu Li
Summary: Inhibiting bacterial growth on biomedical implants is clinically significant. This study presents a surface-immobilized multifunctional coating based on a hybrid peptide, which exhibits effective antimicrobial and antifouling properties without cytotoxicity. In vivo studies demonstrate the effectiveness of this coating in reducing subcutaneous infections.
APPLIED MATERIALS TODAY
(2022)
Review
Medicine, General & Internal
Chen-Xuan Wei, Michael Francis Burrow, Michael George Botelho, W. Keung Leung
Summary: Recent technological and analytical breakthroughs have enabled studies on small quantity, highly complex protein samples, such as salivary pellicle. Advances in mass spectrometry-based computational proteomics allow precise identification and quantification of complex protein samples, facilitating the determination of corresponding genes and cellular functions at the protein level through protein-protein interaction mapping. This has led to the breaking of barriers in research on small-quantity-complex-protein samples such as salivary pellicle.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Dentistry, Oral Surgery & Medicine
Darren Dhananthat Chawhuaveang, Ollie Yiru Yu, Iris Xiaoxue Yin, Walter Yu-Hang Lam, May Lei Mei, Chun-Hung Chu
Summary: Acquired salivary pellicle is a dynamic process of macromolecular adsorption and desorption that plays a crucial role in the development of oral diseases. It acts as a natural barrier to protect the tooth surface, modulates calcium and phosphate concentrations, influences the initial colonizer of oral biofilm, and affects the transportation pathway of acidic products, contributing to the prevention of dental caries and periodontal disease.
JOURNAL OF DENTAL SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Vinicius Taioqui Pela, Marilia Afonso Rabelo Buzalaf, Samira Helena Niemeyer, Tommy Baumann, Flavio Henrique-Silva, Danyelle Toyama, Edson Crusca, Reinaldo Marchetto, Adrian Lussi, Thiago Saads Carvalho
Summary: This study investigated the protective effect of different proteins/peptides on native human enamel by direct action on the enamel surface and modifying the salivary pellicle. The combination of all three proteins/peptides demonstrated the best protective effect, offering a new direction for the prevention of dental erosion.
JOURNAL OF DENTISTRY
(2021)
Review
Biochemistry & Molecular Biology
Katarina Jansakova, Klaudia Kyselicova, Daniela Ostatnikova, Gabriela Repiska
Summary: Saliva, as a potential alternative to other body fluids, offers a complex view on the current state of the individual with its sources of nucleic acid of both human and microbial origin, protein, and non-protein molecules. Using salivary markers may simplify ASD screening and potentially reveal the etiopathogenesis of ASD, with the advantage of easier recruitment of neurotypical counterparts for studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biophysics
Nicholas G. Fischer, Conrado Aparicio
Summary: This article provides an overview of salivary pellicle formation processes and its impacts on dental biomaterials, discussing measurement techniques and factors that may affect its formation. Future research may lead to rationally designed dental biomaterials to control the salivary pellicle and enhance material function and patient outcomes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Infectious Diseases
Andrew R. Dentino, DongHwa Lee, Kelley Dentino, Arndt Guentsch, Mohammadreza Tahriri
Summary: The study showed that as the density of phosphate in polymers increased, the metabolism of biofilms decreased. Loading CPC on phosphate disks significantly reduced biofilm metabolism, while a salivary pellicle only enhanced the antimicrobial activity of CPC on uncharged PMMA surfaces.
Article
Microbiology
Carolina Robertsson, Gunnel Svensater, Julia R. Davies, Anders Bay Nord, Daniel Malmodin, Claes Wickstrom
Summary: Bacterial metabolism in oral biofilms involves complex networks and regulations, including interactions with host components. In this study, it was found that two oral commensal bacteria, S. gordonii and Actinomyces naeslundii, can utilize salivary MUC5B as a nutrient source during early biofilm formation. The presence of S. gordonii promotes the colonization of A. naeslundii on MUC5B, and synergistic effects were observed in the dual species biofilm metabolism.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Markus Reise, Stefan Kranz, Markus Heyder, Julius Beck, Christian Roth, Andre Guellmar, Ferdinand von Eggeling, Ulrich Schubert, Bettina Loeffler, Bernd Sigusch, Marek M. Kowalczuk
Summary: The study investigated the proteomic profile of salivary pellicles formed on different dental composites, revealing that pellicle formation was material- and time-dependent, with differences in proteins among the composites and controls.
Article
Biotechnology & Applied Microbiology
Kevin Liu, Xue-Jun Kong
Summary: This study aimed to evaluate the impact of oral probiotic supplementation on the salivary microbiota composition of individuals with PWS and its association with growth parameters and behavioral symptoms. The study found that the probiotic-treated group exhibited higher salivary microbiome alpha-diversity and certain microbial groups were associated with anti-inflammatory, anti-obesity, toxin degradation, and anti-oxidative injury effects. Additionally, some oral taxa were correlated with the severity of social behaviors.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Microbiology
Gabriella Boisen, Julia R. Davies, Jessica Neilands
Summary: The study found that biofilm formation significantly increased acid tolerance in oral streptococcal and Actinomyces species. Salivary proteins enhanced acid tolerance development and ATR induction in S. gordonii biofilms. Other bacteria's response to salivary pellicle was not as significant.
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Multidisciplinary Sciences
Isamu Kado, Ryo Kunimatsu, Yuki Yoshimi, Cynthia Concepcion Medina, Sakura Yamada, Kotaro Tanimoto
Summary: Saliva plays a crucial role in masticatory function and caries prevention. Studies on saliva properties have shown that males have significantly higher saliva flow rate, pH, and buffering capacity than females, although the Streptococcus mutans score is higher in females and positively correlates with saliva flow rate.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Boyang Qin, Chenyi Fei, Bruce Wang, Howard A. Stone, Ned S. Wingreen, Bonnie L. Bassler
Summary: Bacterial cells can self-organize into structured communities at fluid-fluid interfaces, forming pellicles - soft living materials composed of cells and extracellular matrix. The time evolution of pellicle formation depends on the initial heterogeneity of the film microstructure, with different starting bacterial seeding densities producing variations in morphogenic stages. Despite global architectural transitions, individual microcolonies remain spatially segregated, maintaining spatial and genetic heterogeneity within the community.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Beata Cizmarova, Vladimira Tomeckova, Beata Hubkova, Anna Hurajtova, Jana Ohlasova, Anna Birkova
Summary: Homeostasis is a crucial regulatory process that maintains a stable internal environment in the human body. Our study emphasizes the importance of salivary antioxidants in reflecting dynamic changes and serving as valuable biomarkers for diagnosing and predicting oral and systemic health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Vuokko Loimaranta, Jussi Hepojoki, Olli Laaksoaho, Arto T. Pulliainen
JOURNAL OF LEUKOCYTE BIOLOGY
(2018)
Article
Biochemical Research Methods
Teemu Haikarainen, Vuokko Loimaranta, Carlos Prieto-Lopez, Pradeep Battula, Jukka Finne, Anastassios C. Papageorgiou
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2013)
Article
Immunology
Martin Parnov Reichhardt, Hanna Jarva, Mark de Been, Juan Miguel Rodriguez, Esther Jimenez Quintana, Vuokko Loimaranta, Willem Meindert de Vos, Seppo Meri
JOURNAL OF IMMUNOLOGY
(2014)
Article
Multidisciplinary Sciences
Martin Parnov Reichhardt, Hanna Jarva, Anna Inkeri Lokki, Hannele Laivuori, Piia Vuorela, Vuokko Loimaranta, Andreas Glasner, Monika Siwetz, Berthold Huppertz, Seppo Meri
Article
Biochemical Research Methods
Vuokko Loimaranta, Karoliina Sievi, Jill Werner, Michael Pawlita, Tim Waterboer, Julia Butt, Stina Syrjanen
JOURNAL OF VIROLOGICAL METHODS
(2019)
Article
Microbiology
Vuokko Loimaranta, Danuta Mazurel, Dongmei Deng, Eva Soderling
Article
Engineering, Chemical
Erika Saaskilahti, Stina Syrjanen, Vuokko Loimaranta, Karolina Louvanto
Summary: Hinokitiol has anti-tumor activity on HPV positive and negative cell lines, inhibiting metabolic activity and DNA synthesis in all carcinoma cell lines, with a more significant effect on HPV-positive cell lines. It induces G1 cell cycle arrest and upregulation of p21 expression, showing potential in preventing the progression of immortalized cells towards malignancy and influencing HPV-associated lesion progression by downregulating E6 and E7 expression.
Article
Dentistry, Oral Surgery & Medicine
Yigit Firatli, Erhan Firatli, Vuokko Loimaranta, Samira Elmanfi, Ulvi K. Gursoy
Summary: This study evaluated the effects of periodontitis-associated bacteria and inflammatory mediators on oral cells, and found that the expression of MCPIP-1 and MALT-1 proteins and mRNA in oral cells is regulated by these factors.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Immunology
Emilia Lonnqvist, Kirsi Grondahl-Yli-Hannuksela, Vuokko Loimaranta, Jaana Vuopio
Summary: This study investigated IgA responses against GAS and oral streptococci from saliva samples of healthy Finnish adults and examined asymptomatic GAS throat carriage.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2022)
Review
Dentistry, Oral Surgery & Medicine
Jukka Huokuna, Vuokko Loimaranta, Merja A. Laine, Anna-Liisa Svedstrom-Oristo
Summary: Orthodontic forces may lead to reduced pulpal blood flow and decreased tooth sensibility, as evidenced by increased response thresholds and negative responses to tooth sensibility tests. There are also increased levels of enzymes and neuropeptides associated with hypoxia and inflammation. Fibrotic tissue formation in the pulp has also been reported.
ACTA ODONTOLOGICA SCANDINAVICA
(2023)
Article
Biochemistry & Molecular Biology
Mervi Gursoy, Jaana Rautava, Pirkko Pussinen, Anna Karin Kristoffersen, Morten Enersen, Vuokko Loimaranta, Ulvi Kahraman Gursoy
Summary: This study evaluated the levels of IgA and IgG antibodies against Porphyromonas gingivalis, Tannerella forsythia, Aggregatibacter actinomycetemcomitans, and Prevotella intermedia in the saliva of Crohn's disease patients. Higher levels of salivary IgG2 and IgG3 antibodies, as well as IgG antibodies against P. gingivalis, A. actinomycetemcomitans, and P. intermedia, were detected in Crohn's disease patients. The carriage of P. gingivalis in the saliva was lower in Crohn's disease patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Tiina Pirttila, Stina Syrjanen, Karolina Louvanto, Vuokko Loimaranta
Summary: Oral infections with high-risk HPV genotypes are associated with certain head and neck squamous cell carcinomas. Such infections can result from oral sex or horizontal transmission from mouth to mouth. In these cases, saliva can serve as a vehicle for HPV transmission. However, the prevalence and dynamics of salivary HPV antibodies in healthy non-vaccinated individuals are not well-known, and the role of salivary antibodies in protection against oral HPV infection is unclear. This study evaluated the levels and correlation of oral HPV16 infection and HPV16L1 and E7 specific antibody levels in saliva and serum samples among 39 women, 13 of whom had persistent oral HPV16 infection.
Article
Microbiology
Basar Karaca, Ozan Haliscelik, Mervi Gursoy, Fadime Kiran, Vuokko Loimaranta, Eva Soderling, Ulvi Kahraman Gursoy
Summary: This study characterized the exopolysaccharides of Lactobacillus plantarum EIR/IF-1 postbiotics and evaluated their antibiofilm activity against oral bacteria. The results showed that the exopolysaccharides exhibited antibiofilm activity by reducing bacterial aggregation and hydrocarbon binding. The findings suggest that Lactobacillus plantarum exopolysaccharides may have potential as a preventive agent against biofilm formation.
Article
Biology
Martin P. Reichhardt, Vuokko Loimaranta, Susan M. Lea, Steven Johnson
LIFE SCIENCE ALLIANCE
(2020)
Article
Food Science & Technology
Pirjo Rantamaki, Vuokko Loimaranta, Erkki Vasara, Jari Latva-Koivisto, Hannu Korhonen, Jorma Tenovuo, Pertti Marnila
FOOD QUALITY AND SAFETY
(2019)