Article
Environmental Sciences
Hyeon-Jeong Lee, Jonghwa Jin, Yoondam Seo, Inseon Kang, Junghyun Son, Eugene C. Yi, Hophil Min
Summary: This study explores the metabolic profiles of rats exposed to DEHP, revealing the specific toxic effects of DEHP based on sex and sexual maturity. The study shows disruptions to steroid hormone and primary bile acid biosynthesis, as well as associations with heart, liver, and kidney damage. The findings contribute to our understanding of the health risks associated with DEHP exposure.
Article
Oncology
Xiangping Lin, Lucie Lecuyer, Xinyu Liu, Mohamed N. Triba, Melanie Deschasaux-Tanguy, Aicha Demidem, Zhicheng Liu, Tony Palama, Adrien Rossary, Marie-Paule Vasson, Serge Hercberg, Pilar Galan, Philippe Savarin, Guowang Xu, Mathilde Touvier
Summary: Correct identification of subjects at high risk is crucial for preventing and screening prostate cancer. Metabolite analysis in biofluids has shown potential in identifying novel biomarkers for PCa. The study found dysregulation of amino acids and sphingolipid metabolism to be associated with future risk of PCa, highlighting 10 specific metabolites.
Article
Chemistry, Analytical
Tuba Recber, Emirhan Nemutlu, Kemal Beksac, Omer Cennet, Volkan Kaynaroglu, Sercan Aksoy, Sedef Kir
Summary: Early diagnosis and effective treatment are crucial for the survival of breast cancer patients. Metabolomic studies can aid in discovering new biomarkers, as shown in this study where a new method was developed to find early detection biomarkers from plasma. The study identified 35 metabolites significantly altered between healthy volunteers and breast cancer patients of different stages, suggesting their potential as biomarkers for early diagnosis.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Shai Dagan, Dana Marder, Nitzan Tzanani, Eyal Drug, Hagit Prihed, Lilach Yishai-Aviram
Summary: This study investigated the impact of matrix complexity on nontargeted detection by LC-MS/MS (Orbitrap) analysis. The results showed that detection limits for nontargets were higher in complex matrices and required higher concentrations. A quantitative method was used to assess and compare the complexity of matrices and predict the typical detection limits of unknowns.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
John Quinones, Rommy Diaz, Jorge F. Beltran, Lidiana Velazquez, David Cancino, Erwin Munoz, Patricio Dantagnan, Adrian Hernandez, Nestor Sepulveda, Jorge G. Farias
Summary: This study analyzed the effects of different feeds on the lipid composition of juvenile Oncorhynchus mykiss muscle and found that the inclusion of Brassica napus oil significantly increased certain lipid levels, while Durvillaea antarctica meal had a less significant impact on lipid variation.
Article
Medicine, Research & Experimental
Cheng Hu, Tao Wang, Xiaoyu Zhuang, Qiaoli Sun, Xiaochun Wang, Hui Lin, Mingli Feng, Jiaqi Zhang, Qin Cao, Yuanye Jiang
Summary: Through high-throughput mass spectrometry analysis, this study identified 15 serum metabolites strongly associated with early-stage NAFLD, which may have significant clinical implications for early diagnosis and monitoring response to therapeutic interventions for NAFLD.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Food Science & Technology
Pufan Zheng, Mei Zhang, Xin Fang, Lili Tang, Zhixue Wang, Fuchen Shi
Summary: This study identified and quantified metabolites in three pear species using a targeted LC-MS/MS approach. The results showed distinct differences in metabolite composition between cultivars, and identified metabolic pathways related to fruit quality. D-xylose, formononetin, procyanidin A1 and beta-nicotinamide mononucleotide were identified as major differentially expressed metabolites.
Article
Biochemical Research Methods
Xinxin Ye, Bin Zhu, Yang Chen, Yingfeng Wang, Dan Wang, Zhigang Zhao, Zhongfeng Li
Summary: This study integrated metabolomics and lipidomics analysis to identify 49 metabolite and 68 lipid biomarkers associated with the pathogenesis of cerebral infarction. A support vector machine model constructed using these biomarkers showed excellent predictive ability for early diagnosis.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Xinxin Ye, Bin Zhu, Yang Chen, Yingfeng Wang, Dan Wang, Zhigang Zhao, Zhongfeng Li
Summary: This study performed integrated metabolomics and lipidomics analysis on healthy subjects and cerebral infarction patients, identifying metabolite and lipid biomarkers and enriching disturbed pathways. The results suggest that the approach could contribute to understanding the metabolic dysregulation associated with cerebral infarction pathogenesis and may aid in its early diagnosis.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Food Science & Technology
Mohamed A. Farag, Amr Abdelwareth, Ahmed Zayed, Tarek F. Eissa, Eric Dokalahy, Andrej Frolov, Ludger A. Wessjohann
Summary: This study investigated the metabolites difference of Egyptian mango using comparative profiling and fingerprinting. The results showed that cultivar had a greater effect on fruit classification than geographical origin. Awees (AS) cultivar had the highest phenolic content, while Langara (LI) cultivar exhibited the strongest antioxidant effect.
Article
Genetics & Heredity
Hao Liu, Jiang Zhu, Qiu Li, Dongjuan Wang, Kexing Wan, Zhaojian Yuan, Juan Zhang, Lin Zou, Xiaoyan He, Jingkun Miao
Summary: Metabolomics was used to successfully differentiate IMDs patients from controls, revealing a broader metabolic spectrum in patients. Several potential novel markers were identified, and the metabolic pathways of IMDs were explored in depth.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2021)
Article
Plant Sciences
Dana Atoum, Ignacio Fernandez-Pastor, Louise Young, RuAngelie Edrada-Ebel
Summary: This study compared the metabolomic profile and biological activity of Jordanian and European chamomile grown under different environmental conditions. European chamomile exhibited anticancer and antioxidant activity, while Jordanian chamomile only showed antioxidant activity. Both chamomile varieties contained dicaffeoylquinic acid isomers with antioxidant capability. Additionally, European chamomile contained chrysosplenetin and apigenin, which displayed anticancer activity.
Article
Biochemistry & Molecular Biology
Zhaoxin Li, Yunlong Cheng, Jinxin Chen, Weijun Xu, Wentao Ma, Sheng Li, Erxia Du
Summary: Insect metabolites are essential for regulating the physiology, behavior, and adaptations of insects, but the systematic metabolomics within insects remains unclear. This study used HPLC-MS/MS technology to perform a comprehensive metabolomics analysis and constructed an integrated metabolic database of nine insect species with different metamorphosis types. A total of 1442 metabolites were identified, including amino acids, organic acids, fatty acids, glycerophospholipids, nucleotides, and benzene derivatives. The metabolites were enriched in important pathways related to arachidonic acid metabolism, tyrosine metabolism, phenylalanine metabolism, and insect hormone biosynthesis. The study revealed a correlation between the evolutionary relationships of species and the types of metabolites present, while the quantities of metabolites varied greatly among species. The metabolome of these representative insects provides a valuable platform for studying insect systemic metabolites and biological events at the metabolic level.
Article
Biochemical Research Methods
Samuel Bernardo-Bermejo, Jingchuan Xue, Linh Hoang, Elizabeth Billings, Bill Webb, M. Willy Honders, Sanne Venneker, Bram Heijs, Maria Castro-Puyana, Maria Luisa Marina, Erik B. van den Akker, Marieke Griffioen, Gary Siuzdak, Martin Giera, Elena Sanchez-Lopez
Summary: Analytical techniques with high sensitivity and selectivity are crucial in clinical sample analysis. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is the standard method, but it is expensive. This study demonstrates that similar results can be achieved using a simpler single mass spectrometry detector combined with enhanced in-source fragmentation/annotation and correlated ion monitoring. The protocol provides step-by-step instructions for optimizing the conditions for this technique, making it broadly applicable and cost-effective for quantifying small molecules.
Article
Biochemistry & Molecular Biology
Jingtong Zhao, Meng Liu, Tongfei Shi, Mohan Gao, Yuqian Lv, Yawei Zhao, Jing Li, Ming Zhang, Hansi Zhang, Fengying Guan, Kan He, Li Chen
Summary: Osteoarthritis is a common multifactorial chronic disease that affects articular cartilage, subchondral bone, and periarticular tissue. The pathogenesis of OA is still unclear. A study using LC/MS-based metabolomics in male rats identified six biomarkers related to tryptophan metabolism, glutamate metabolism, nitrogen metabolism, spermidine metabolism, and fatty acid metabolism pathways in distinguishing OA and control groups. These metabolites provide insight into the metabolic mechanisms involved in OA progression.
Article
Biochemical Research Methods
Seon-Kyu Kim, Jong Hwan Kim, Seok-Joong Yun, Wun-Jae Kim, Seon-Young Kim
Article
Urology & Nephrology
Ho Won Kang, Sang Keun Lee, Won Tae Kim, Yong-June Kim, Seok-Joong Yun, Sang-Cheol Lee, Wun-Jae Kim
JOURNAL OF ENDOUROLOGY
(2014)
Article
Oncology
Ho Won Kang, Dong-Un Tchey, Chunri Yan, Won Tae Kim, Yong-June Kim, Seok-Joong Yun, Sang-Cheol Lee, Yung Hyun Choi, Isaac Yi Kim, Wun-Jae Kim
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2014)
Article
Urology & Nephrology
Ho Won Kang, Sung Pil Seo, Won Tae Kim, Yong-June Kim, Seok-Joong Yun, Sang-Cheol Lee, Wun-Jae Kim
Article
Urology & Nephrology
Ho Won Kang, Sung Pil Seo, Whi-An Kwon, Seung Hyo Woo, Won Tae Kim, Yong-June Kim, Seok-Joong Yun, Sang-Cheol Lee, Wun-Jae Kim
Article
Oncology
Yun-Sok Ha, Geun Taek Lee, Ye-Hwan Kim, Se Yun Kwon, Seock Hwan Choi, Tae-Hwan Kim, Tae Gyun Kwon, Seok Joong Yun, Isaac Yi Kim, Wun-Jae Kim
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2014)
Article
Medicine, General & Internal
Won Tae Kim, Yong-June Kim, Seok Joong Yun, Kyung-Sub Shin, Young Deuk Choi, Sang Cheol Lee, Wun-Jae Kim
YONSEI MEDICAL JOURNAL
(2014)
Review
Biochemistry & Molecular Biology
Xuan-Mei Piao, Eun-Jong Cha, Seok Joong Yun, Wun-Jae Kim
Summary: Bladder cancer is the most common neoplasm of the urinary tract, and liquid biopsy has emerged as a promising field of cancer research. Liquid biopsy samples offer comprehensive information and enable tracking of genetic evolution of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
So Young Kim, Hyun Hwangbo, Min Yeong Kim, Seon Yeong Ji, Da Hye Kim, Hyesook Lee, Gi-Young Kim, Sung-Kwon Moon, Sun-Hee Leem, Seok Joong Yun, Wun-Jae Kim, JaeHun Cheong, Cheol Park, Yung Hyun Choi
Summary: The study demonstrates that betulinic acid induces apoptosis, necrosis, and cell cycle arrest in bladder cancer cells through modulation of cell cycle regulators and mitochondrial function, leading to decreased proliferation and migration.
Article
Biochemistry & Molecular Biology
Xuan-Mei Piao, Chaelin You, Young Joon Byun, Ho Won Kang, Junho Noh, Jaehyun Lee, Hee Youn Lee, Kyeong Kim, Won Tae Kim, Seok Joong Yun, Sang-Cheol Lee, Kyuho Kang, Yong-June Kim
Summary: NMIBC is a common disease with high recurrence rate, which may progress to the lethal form MIBC. BUB1, a mitotic checkpoint kinase, has been identified as a potential prognostic biomarker for NMIBC by promoting cell proliferation through regulating the G2/M transition of the cell cycle. Further validation in internal and external cohorts showed its correlation with NMIBC progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Eun Hye Lee, Jae-Wook Chung, Eunji Sung, Bo Hyun Yoon, Minji Jeon, Song Park, So Young Chun, Jun Nyung Lee, Bum Soo Kim, Hyun Tae Kim, Tae Hwan Kim, Seock Hwan Choi, Eun Sang Yoo, Tae Gyun Kwon, Ho Won Kang, Wun-Jae Kim, Seok Joong Yun, Sangkyu Lee, Yun-Sok Ha
Summary: This study evaluated the role of PDK4 in bladder cancer and its related protein changes. It was found that elevated expression of PDK4 was observed in high-grade bladder cancers. Knockdown of PDK4 in bladder cancer cells resulted in reduced cell migration and invasion. Additionally, a PDK4 knockdown xenograft model showed reduced bladder cancer growth. These findings suggest that PDK4 plays a critical role in the metastasis and growth of bladder cancer cells through changes in ERK, SRC, and JNK.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xuan-Mei Piao, Seon-Kyu Kim, Young Joon Byun, Chuang-Ming Zheng, Ho Won Kang, Won Tae Kim, Yong-June Kim, Sang-Cheol Lee, Wun-Jae Kim, Sung-Kwon Moon, Yung Hyun Choi, Seok Joong Yun
Summary: This study compared the utility of different risk assessments in NMIBC patients and found that the molecular signature-based subtype predictor (MSP888) had independent predictive value for NMIBC progression, especially in patients without intravesical BCG immunotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Cheol Park, Hee-Jae Cha, Min Yeong Kim, EunJin Bang, Sung-Kwon Moon, Seok Joong Yun, Wun-Jae Kim, Jeong Sook Noh, Gi-Young Kim, Suengmok Cho, Hyesook Lee, Yung Hyun Choi
Summary: The study found that phloroglucinol can protect human retinal pigment epithelium cells from oxidative damage, reduce apoptosis, and improve cell function by decreasing ROS production.
Meeting Abstract
Urology & Nephrology
S. J. Yun, H. W. Kang, W. T. Kim, Y-T. Kim, S-C. Lee, W-J. Kim