Article
Multidisciplinary Sciences
Gang Cheng, Micael Hardy, Balaraman Kalyanaraman
Summary: N-acetylcysteine (NAC) is an antioxidant drug that has been used in tumor cells and preclinical mice tumor xenografts. A new thiol-containing molecule, Mito(10)-NAC, has been synthesized and found to be nearly 2000-fold more effective than NAC in inhibiting cancer cell proliferation, including pancreatic cancer cells.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Yi Rao, Seth T. Gammon, Margie N. Sutton, Niki M. Zacharias, Pratip Bhattacharya, David Piwnica-Worms
Summary: This study reveals that high concentrations of exogenous pyruvate inhibit intracellular LDH activity and glycolytic ECAR, with the extent of inhibition being highly dependent on pyruvate influx mediated by monocarboxylate transporter-1. These findings suggest that maintaining plasma pyruvate at submillimolar concentrations could minimize transient metabolic perturbations and enhance the accuracy of metabolic studies using hyperpolarized [1-C-13]pyruvate magnetic resonance spectroscopic imaging and stable isotope tracer experiments.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Dane T. Sessions, David F. Kashatus
Summary: Many tumors are heterogeneous cell populations composed of a minority of epithelial-like cancer stem cells (CSCs), which exhibit distinct metabolic signatures and phenotypes arising from altered mitochondrial function and turnover regulated by constant cycles of fusion and fission. The protein machinery driving mitochondrial dynamics is relatively simple, providing potential new therapeutic avenues to target CSC metabolism and selectively eradicate tumor-generating cells to reduce the risks of metastasis and relapse. Mitochondrial recycling through mitophagy in CSCs is associated with the maintenance of reactive oxygen species levels that dictate gene expression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ivan Ponce, Nelson Garrido, Nicolas Tobar, Francisco Melo, Patricio C. Smith, Jorge Martinez
Summary: The research revealed that increased tissue stiffness stimulates lactate production and glucose uptake by mammary fibroblasts, which is correlated with the expression of specific proteins. Additionally, mammary stromal cells cultured on stiff matrices generated factors that stimulated epithelial breast migration.
Article
Oncology
Arne Van der Vreken, Inge Oudaert, Gamze Ates, Sylvia Faict, Philip Vlummens, Hatice Satilmis, Rong Fan, Anke Maes, Ann Massie, Kim De Veirman, Elke De Bruyne, Karin Vanderkerken, Eline Menu
Summary: This study suggests an alternative combination treatment for multiple myeloma by targeting metabolic pathways. The combination of syrosingopine and metformin shows strong cytotoxic effects on multiple myeloma cell lines and patient samples, suppressing both glycolysis and oxidative phosphorylation. Furthermore, the combination treatment leads to a significant reduction in tumor burden in vivo.
JOURNAL OF PATHOLOGY
(2023)
Article
Multidisciplinary Sciences
Vicente Medel, Nicolas Crossley, Ivana Gajardo, Eli Muller, L. Felipe Barros, James M. Shine, Jimena Sierralta
Summary: By analyzing gene expression data, we found that neuronal MCT2 lactate transporter is enriched in the cerebral cortex and negatively correlated with cortical thickness. MCT2 expression is associated with glucose utilization and brain function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Alexandra Poetzsch, Sara Zocher, Stefanie N. Bernas, Odette Leiter, Annette E. Ruenker, Gerd Kempermann
Summary: The study showed that L-lactate has a pro-proliferative effect on neural precursor cells, which is dependent on the active lactate transport by monocarboxylate transporters and is not linked to amplified mitochondrial respiration. L-lactate redirected glucose metabolism to the pentose phosphate pathway, as indicated by increased glucose-6-phosphate dehydrogenase activity and decreased glycolysis.
Article
Cell Biology
Bessem Mornagui, Raja Rezg, Cendrine Repond, Luc Pellerin
Summary: Bisphenol S (BPS), a substitute for bisphenol A (BPA), may act as an obesogenic endocrine disruptor and affect monocarboxylate transporters and mitochondrial respiratory system in the liver, leading to the development of hepatic steatosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Public, Environmental & Occupational Health
Hamed Hatami, Atefe Sajedi, Seyed Mostafa Mir, Mohammad Yousef Memar
Summary: This article reviews the importance of glycolysis and lactate in cancer cells, highlighting their role in metastasis and invasion. Metabolic changes, including increased glycolysis, play a significant role in cancer cell survival and growth. Lactate dehydrogenase and monocarboxylate transporters are important proteins involved in lactate production and transport, aiding in immune system evasion and cancer cell expansion.
HEALTH SCIENCE REPORTS
(2023)
Review
Cell Biology
Qixin Duan, Shuang Zhang, Yang Wang, Dongming Lu, Yingming Sun, Yongyang Wu
Summary: The Warburg effect refers to the hyperactive glycolysis in tumor cells that provides energy for rapid growth and proliferation even under sufficient oxygen. Proton-coupled monocarboxylate transporters (MCTs) play a crucial role in the metabolic reprogramming and survival of tumor cells, as well as tumor angiogenesis, immune suppression, multidrug resistance, migration and metastasis, ferroptosis resistance, and autophagy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marta Baczewska, Elzbieta Supruniuk, Klaudia Bojczuk, Pawel Guzik, Patrycja Milewska, Katarzyna Kononczuk, Jakub Dobroch, Adrian Chabowski, Pawel Knapp
Summary: The study aimed to assess the dominant energy substrate transport mechanism in ovarian cancer cells and predict clinical outcomes using genomic aberrations. The researchers found differential gene expression in high-grade serous carcinoma and suggested the potential use of glucose and lactate transport as therapeutic targets to impede ovarian cancer growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Sergey Leonov, Olumide Inyang, Konstantin Achkasov, Elizaveta Bogdan, Elizaveta Kontareva, Yongheng Chen, Ying Fu, Andreyan N. N. Osipov, Margarita Pustovalova, Yulia Merkher
Summary: Metastasis is the leading cause of cancer-related deaths, and predicting its occurrence is crucial for improving survival rates. Current prediction methods, such as lymph-node status and genetic testing, have limitations and may take weeks to obtain results. New mechanobiology-related techniques based on the mechanical invasiveness of cancer cells have shown promise in detecting metastasis propensity. However, they are still complex and require further exploration. Investigating novel markers related to the mechanobiological properties of tumor cells may significantly impact the prognosis of metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ophthalmology
Victor Calbiague Garcia, Yiyi Chen, Barbara Cadiz, Lan Wang, Francois Paquet-Durand, Oliver Schmachtenberg
Summary: In this study, lactate and glucose nanosensors were expressed in mouse retinal explants to study metabolite flux. The functional expression of monocarboxylate transporters (MCTs) in Muller cells of retinal explants was revealed through pharmacological manipulation and immunohistochemistry. The introduction of FRET nanosensors to measure key metabolites at the cellular level may contribute to a better understanding of retinal metabolism.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ehsan Sohrabi, Masoumeh Moslemi, Ehsan Rezaie, Nahid Nafissi, Mansoor Khaledi, Hamed Afkhami, Javad Fathi, Ali Zekri
Summary: The study evaluated the expression of MCT3 (SLC16A8), MCT8 (SLC16A2), and MCT9 (SLC16A9) genes in breast cancer samples and compared to normal adjacent tissues. The results showed significantly lower expression of the MCT3 gene in breast cancer tissue, suggesting potential as a therapeutic target in breast cancer patients.
Article
Biochemistry & Molecular Biology
Maria Angela Masini, Valentina Bonetto, Marcello Manfredi, Anna Pasto, Elettra Barberis, Sara Timo, Virginia Vita Vanella, Elisa Robotti, Francesca Masetto, Francesca Andreoli, Alessandra Fiore, Sara Tavella, Antonio Sica, Massimo Donadelli, Emilio Marengo
Summary: The absence of gravity has significant impact on cancer cells, affecting their morphology, metabolism, and phenotype. Simulated microgravity can activate cellular motility pathways and enhance the migratory capability of tumor cells. Additionally, the absence of gravity can alter metabolic pathways, promoting the survival and proliferation of cancer cells.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Oncology
Vanitha N. Sivalingam, Ayse Latif, Sarah Kitson, Rhona McVey, Katherine G. Finegan, Kay Marshall, Michael P. Lisanti, Federica Sotgia, Ian J. Stratford, Emma J. Crosbie
BRITISH JOURNAL OF CANCER
(2020)
Review
Biochemistry & Molecular Biology
T. Volpari, F. De Santis, A. P. Bracken, S. M. Pupa, M. Buschbeck, A. Wegner, S. Di Cosimo, Mp. Lisanti, G. Dotti, M. Massaia, G. Pruneri, A. Anichini, O. Fortunato, F. De Braud, M. Del Vecchio, M. Di Nicola
CYTOKINE & GROWTH FACTOR REVIEWS
(2020)
Article
Cell Biology
Bela Ozsvari, Federica Sotgia, Michael P. Lisanti
Article
Cell Biology
Camillo Sargiacomo, Federica Sotgia, Michael P. Lisanti
Review
Biochemistry & Molecular Biology
Francesca De Santis, Giovanni Fuca, Dirk Schadendorf, Alberto Mantovani, Luca Magnani, Michael Lisanti, Stephen Pettitt, Matteo Bellone, Giannino Del Sal, Saverio Minucci, Alexander Eggermont, Paolo Bruzzi, Silvio Bicciato, Pierfranco Conte, Roberta Noberini, John Hiscott, Filippo De Braud, Michele Del Vecchio, Massimo Di Nicola
Summary: Experts at the Tenth Edition of the Annual Congress on Anticancer Innovative Therapy shared the latest knowledge and novel therapeutic approaches in fields such as immuno-oncology, epigenetics, cancer metabolism, cancer stem cells, tumor cell signaling, and the immune system. The conference also discussed possible mechanisms of resistance to these innovative therapies, particularly with respect to immune checkpoint blockers (ICB), providing a broad overview of future challenges and hopes for improving cancer treatment in the medium-short term.
CYTOKINE & GROWTH FACTOR REVIEWS
(2021)
Article
Multidisciplinary Sciences
Cristian Scatena, Giovanni Fanelli, Giuseppe Nicolo Fanelli, Michele Menicagli, Paolo Aretini, Valerio Ortenzi, Sara Piera Civitelli, Lorenzo Innocenti, Federica Sotgia, Michael P. Lisanti, Antonio Giuseppe Naccarato
Summary: Recent evidence indicates that loss of caveolin expression in the stromal compartment of human invasive breast carcinoma may be predictive of disease recurrence, metastasis, and poor prognosis. Additionally, a study on sCav-1 expression in breast cancer and axillary lymph nodes suggests that the loss of Cav-1 in lymph nodes may contribute to metastatic spread in breast cancer.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Marco Fiorillo, Cristian Scatena, Antonio Giuseppe Naccarato, Federica Sotgia, Michael P. Lisanti
Summary: This study identifies high ATP production by the mitochondrial ATP-synthase as a new therapeutic target for anticancer therapy, particularly for inhibiting tumor progression. ATP-high cancer cells exhibit aggressive phenotypes with increased proliferation, stemness, anchorage-independence, cell migration, invasion, and multi-drug resistance, along with high antioxidant capacity. ATP depletion through targeting ATP5F1C may serve as a promising strategy for preventing metastasis and avoiding treatment failure, making ATP5F1C a potential biomarker and molecular target for future drug development to prevent metastatic disease progression.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Camillo Sargiacomo, Sophie Stonehouse, Zahra Moftakhar, Federica Sotgia, Michael P. Lisanti
Summary: MTDR can be used to target and eradicate cancer stem cells by selectively interfering with mitochondrial metabolism in breast cancer cells. It significantly inhibits tumor growth and metastasis with little to no toxicity observed in a preclinical animal model.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Filippo Di Pisa, Elisa Pesenti, Maria Bono, Andrea N. Mazzarello, Cinzia Bernardi, Michael P. Lisanti, Giovanni Renzone, Andrea Scaloni, Ermanno Ciccone, Franco Fais, Silvia Bruno, Paolo Scartezzini, Fabio Ghiotto
Summary: This study sheds light on the potential role of SH3BGRL3 in regulating myosin-cytoskeleton interaction and cell migration. It was found that SH3BGRL3 specifically interacts with Myo1c in a Ca2+-dependent manner, suggesting a potential regulatory mechanism for cytoskeleton dynamics involving SH3BGRL3 and Myo1c.
BMC MOLECULAR AND CELL BIOLOGY
(2021)
Review
Oncology
Marco Fiorillo, Bela Ozsvari, Federica Sotgia, Michael P. Lisanti
Summary: The study suggests that high mitochondrial ATP production is a new therapeutic target for cancer treatment, isolating metabolically fit cancer cells with enhanced stem-like properties and invasive abilities. By regulating mitochondrial ATP-synthase, prevention of metastasis in cancer cells is possible.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, General & Internal
Helen Clarke, Michelle Harvie, Cheryl Lombardelli, Suzanne Krizak, Katharine Sellers, Hannah Harrison, Yit Y. Lim, Caroline Parkin, Shruthi Patel, Basil G. Issa, Anthony J. Maxwell, Julie Wisely, John Belcher, Robert Clarke, Anthony Howell, Emma J. Crosbie, Sacha J. Howell
Summary: This study aims to investigate the cellular and molecular changes in breast and endometrial tissue following total diet replacement (TDR)-induced weight loss in high-risk women, as well as adherence to a 12-month TDR weight loss intervention. The findings of this study are important for understanding the role of TDR in the prevention and management of breast and endometrial cancer.
Article
Biochemistry & Molecular Biology
Matteo Brindisi, Luca Frattaruolo, Marco Fiorillo, Vincenza Dolce, Federica Sotgia, Michael P. Lisanti, Anna Rita Cappello
Summary: This study found that elevated cholesterol levels increase the aggressiveness of breast cancer cells and promote the release of pro-inflammatory factors. Furthermore, there is a close symbiotic relationship between breast cancer cells and the microenvironment. These findings highlight the potential of targeting the cholesterol-ERR alpha synergy for breast cancer treatment.
Article
Cell Biology
Sundee Dees, Laura Pontiggia, Jean-Francois Jasmin, Federica Sotgia, Michael P. Lisanti, Isabelle Mercier