Review
Microbiology
Cristina Landeta, Adrian Mejia-Santana
Summary: Antimicrobial resistance is a major global health challenge. Antibacterial discovery research has focused on cell-based and target-based high-throughput assays. The development of innovative screening technologies combining these two methods has provided new insights and potential for the discovery of new antimicrobials.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jan Schlegel, Bartlomiej Porebski, Luca Andronico, Leo Hanke, Steven Edwards, Hjalmar Brismar, Ben Murrell, Gerald M. McInerney, Oscar Fernandez-Capetillo, Erdinc Sezgin
Summary: Speed is crucial in dealing with infectious disease outbreaks, including quickly identifying host binding factors to pathogens. The complexity of the host plasma membrane often hinders fast and accurate determination of these binding factors and high-throughput screening for antimicrobial drug targets. This study presents a multiparametric and high-throughput platform that overcomes this barrier, allowing for rapid screening of host binding factors and discovery of new antiviral drug targets. The platform's sensitivity and robustness were demonstrated by successfully blocking SARS-CoV-2 particles using nanobodies and IgGs from human serum samples.
Review
Chemistry, Multidisciplinary
Farhad Soheilmoghaddam, Madeleine Rumble, Justin Cooper-White
Summary: Many existing clinical treatments are limited in their ability to completely restore decreased or lost tissue and organ function. The increasing demand for new medical interventions has led to the development of promising fields such as gene therapy, tissue engineering, and regenerative medicine. Success in these fields relies on biomaterials that provide necessary biological cues for cell fate, necessitating a shift towards rapid synthesis and characterization practices.
Article
Microbiology
Yong Lai, Liang Cui, Gregory H. Babunovic, Sarah M. Fortune, John G. Doench, Timothy K. Lu
Summary: Shigellosis is a major cause of diarrheal deaths worldwide, especially in children. Understanding how Shigella manipulates macrophages can lead to new therapeutic interventions. High-throughput CRISPR screens provide insights into the complex interactions between macrophages and Shigella, offering potential avenues for treatment.
Article
Multidisciplinary Sciences
Katharina A. Fransen, Sarah H. M. Av-Ron, Tess R. Buchanan, Dylan J. Walsh, Dechen T. Rota, Lana Van Note, Bradley D. Olsen
Summary: The development of biodegradable plastics has been driven by the increasing plastic pollution. However, limited research on polymer biodegradation has hindered innovation due to costly and slow measurement methods. To tackle this issue, a high-throughput polymer synthesis and biodegradation method were utilized to generate a dataset for 642 chemically distinct polyesters and polycarbonates. The results showed that biodegradability depended on the length of the aliphatic repeat unit, with shorter chains and side chains improving biodegradability. Aromatic backbone groups generally hindered biodegradability, but ortho- and para-substituted benzene rings were more degradable than meta-substituted rings. In addition, ether groups in the backbone improved biodegradability.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Surya V. Prabhakar V. Vattikuti, Jie Zeng, Rajavaram Ramaraghavulu, Jaesool Shim, Alain Mauger, Christian M. Julien
Summary: This article mainly introduces the tremendous development of bismuth-based nanostructures (BBNs) in the fields of photocatalysis and electro-catalysis. The methods of optimizing surface engineering to improve charge transport and the separation of available photocatalysts are discussed, and different strategies for enhancing photocatalytic activity are summarized. The surface plasmon resonance effect for photocatalytic enhancement is examined, and the photocatalytic mechanism of bismuth-based photocatalysts is discussed in detail, considering different semiconductor junctions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Matthew Bain, Jose L. Godinez Castellanos, Stephen E. Bradforth
Summary: High-repetition-rate lasers have the potential to revolutionize ultrafast spectroscopy by enabling routine analysis for machine learning models in the design of photochemical syntheses. In this study, we combine innovations in line scan cameras and micro-electro-mechanical grating modulators with high-pressure liquid chromatography pumps to develop a transient absorption spectrometer that can characterize photoreactions in minutes. Additionally, we demonstrate the utility of this technique in exploring the effects of conformational modification on excited-state processes.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Cell Biology
Jana Deitersen, Lena Berning, Fabian Stuhldreier, Sara Ceccacci, David Schluetermann, Annabelle Friedrich, Wenxian Wu, Yadong Sun, Philip Boehler, Niklas Berleth, Maria Jose Mendiburo, Sabine Seggewiss, Ruchika Anand, Andreas S. Reichert, Maria Chiara Monti, Peter Proksch, Bjoern Stork
Summary: High-throughput screening identified arzanol as a novel autophagy-modulating drug that not only induces early autophagosome biogenesis but also inhibits later autophagy events. Arzanol sensitizes bladder cancer cells to cisplatin and induces mitochondrial fragmentation, suggesting its potential as a lead compound for cancer therapy. The compound targets proteins related to mitochondria-associated quinone-binding oxidoreductases, making it a valuable tool for autophagy research.
CELL DEATH & DISEASE
(2021)
Review
Pharmacology & Pharmacy
Fabio Stossi, Pankaj K. Singh, Kazem Safari, Michela Marini, Demetrio Labate, Michael A. Mancini
Summary: Measuring single cell responses to chemicals is crucial for human health, and high throughput, microscopy-based assays coupled with multi-dimensional single cell analysis methods are being used to address this problem. This article summarizes efforts in this field, focusing on environmental toxicology and drug screening, and discusses the advantages and limitations of high throughput assays.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Xabier Rodriguez-Martinez, Enrique Pascual-San-Jose, Mariano Campoy-Quiles
Summary: The discovery of novel high-performing materials in organic solar cells has rapidly increased efficiency, but traditional experimentation methods are unable to evaluate the vast catalog of materials efficiently. High-throughput experimental and computational methods are being utilized to accelerate the discovery of new materials, with machine-learning algorithms playing a key role in retrieving quantitative structure-activity relationships.
ENERGY & ENVIRONMENTAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Sarah E. Pierce, Jeffrey M. Granja, William J. Greenleaf
Summary: The Spear-ATAC method enables simultaneous reading of chromatin accessibility profiles and sgRNA sequences from single cells, revealing the dynamic epigenetic responses to regulatory perturbations in cancer cells. Dynamics of transcription factor binding can drive epigenetic states, leading to a diversity of phenotypes.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Haiyan Wang, Shasha Li, Qianyu Wang, Zhengshuo Jin, Wei Shao, Yan Gao, Lu Li, Kequan Lin, Lin Zhu, Huili Wang, Xuebin Liao, Dong Wang
Summary: Combination immunotherapy using AKIs has been found to reprogram TIP gene expression, promote T cell infiltration in TNBC, and enhance the efficacy of PD-1, suggesting a novel strategy for discovering combination immunotherapy compounds.
Article
Chemistry, Multidisciplinary
Xiao Lu, Zhiji Luo, Ruili Huang, Donald C. Lo, Wenwei Huang
Summary: The efficiency and scalability of detecting the formation of new chemical bonds in high-throughput synthesis is limited by conventional, time-consuming, and labor-intensive methods for product detection. This study presents a miniaturizable purification method using inexpensive equipment to rapidly and efficiently isolate quaternary ammonium-tagged products from reaction mixtures, enabling the establishment of a high-throughput reaction discovery platform. Through validation in a screen of 1536 reactions, one previously unreported transformation was identified.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Oncology
Martin Lang, Laura S. Schmidt, Kelli M. Wilson, Christopher J. Ricketts, Carole Sourbier, Cathy D. Vocke, Darmood Wei, Daniel R. Crooks, Youfeng Yang, Benjamin K. Gibbs, Xiaohu Zhang, Carleen Klumpp-Thomas, Lu Chen, Rajarshi Guha, Marc Ferrer, Crystal McKnight, Zina Itkin, Darawalee Wangsa, Danny Wangsa, Amy James, Simone Difilippantonio, Baktir Karim, Francisco Moris, Thomas Ried, Maria J. Merino, Ramaprasad Srinivasan, Craig J. Thomas, W. Marston Linehan
Summary: The study identified several potential therapeutic agents for treating advanced MiT-RCC, including PI3K/mTOR inhibitor NVP-BGT226, transcription inhibitor Mithramycin A, and GPNMB-targeted antibody-drug conjugate CDX-011. Preclinical studies demonstrated the efficacy of these agents in vitro and in vivo, providing potential treatment options for patients with MiT-driven RCC.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Damiano Cirri, Francesco Bartoli, Alessandro Pratesi, Emma Baglini, Elisabetta Barresi, Tiziano Marzo
Summary: This article provides an overview of research approaches to improve metal-based agents for cancer and infection treatments. Despite the success of approved inorganic drugs in tumor chemotherapy, the discovery and clinical application of new inorganic drugs are slow and limited due to pharmaceutical industry influences.