Article
Chemistry, Multidisciplinary
Petras Venckus, Ieva Endriukaityte, Kotryna Cekuolyte, Renata Gudiukaite, Andrius Pakalniskis, Egle Lastauskiene
Summary: This study tested the effects of silver nanoparticles on Haematococcus pluvialis and found that high concentrations of AgNPs significantly reduced culture productivity in green-stage cultures, while the impact on red-stage cultures was smaller. The addition of AgNPs also led to changes in cell morphology, including deformations, disrupted cytokinesis, and induced sexual reproduction.
Article
Green & Sustainable Science & Technology
Gannoru Kankanamalage Sanuji Hasara Nishshanka, Vinoj Chamilka Liyanaarachchi, Pemaththu Hewa Viraj Nimarshana, Thilini Udayangani Ariyadasa, Jo-Shu Chang
Summary: The microalga Haematococcus pluvialis has high biotechnological potential for producing various valuable compounds. Biorefining, which integrates multiple production processes in a single facility, can maximize the utilization of feedstock and reduce resource consumption. This review discusses the potential of biorefining H. pluvialis to promote more sustainable and cleaner production technologies.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Agricultural Engineering
Yongteng Zhao, Hui-Ping Wang, Chunli Yu, Wei Ding, Benyong Han, Shuxiang Geng, Delu Ning, Ting Ma, Xuya Yu
Summary: The study revealed that melatonin treatment upregulated the biosynthesis of astaxanthin and lipids in Haematococcus pluvialis, affecting the accumulation process through the regulation of gene expression and metabolic pathways. The treatment may promote coproduction of astaxanthin and lipids by regulating the carbon-nitrogen balance and antioxidant system.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jae Been Lee, Yun Hwan Park, Min Seo Jeon, Sok Kim, Yoon-E Choi
Summary: In this study, the researchers successfully increased the production of astaxanthin in Haematococcus pluvialis by combining polyethylenimine (PEI) with chitosan. The polyethylenimine concentration had an effect on astaxanthin accumulation, while the molecular weight of chitosan did not. The treatment also increased the reactive oxygen species content in cells, leading to the upregulation of enzymes involved in astaxanthin biosynthesis. The PEI-chitosan fiber treatment showed high recyclability and maintained over 90% astaxanthin production efficiency.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Hang Guo, Tao Li, Yongteng Zhao, Xuya Yu
Summary: Copper treatment significantly increased astaxanthin and lipid synthesis in Haematococcus pluvialis, leading to upregulation of biosynthesis genes and antioxidant enzyme-related genes, as well as a decrease in reactive oxygen species levels. Additionally, copper induced the activation of protein kinases, facilitating the production of astaxanthin and lipids in the algae.
BIORESOURCE TECHNOLOGY
(2021)
Review
Agricultural Engineering
Bolam Kim, Soo Youn Lee, Aditya Lakshmi Narasimhan, Sangui Kim, You-Kwan Oh
Summary: The green microalga Haematococcus pluvialis is a valuable source of astaxanthin, a powerful antioxidant widely used in various industries. The unique cyst-like cell with a rigid three-layered cell wall formed during astaxanthin accumulation presents challenges for extraction. Various cell disruption methods and downstream processes for sustainable development are also discussed in this review.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hana-Nur Fitriana, Soo-Youn Lee, Sun-A Choi, Ji-Ye Lee, Bo-Lam Kim, Jin-Suk Lee, You-Kwan Oh
Summary: This study investigated the technical feasibility of inducing astaxanthin biosynthesis in H. pluvialis using electric stimulation in a two-chamber bioelectrochemical system. An optimal voltage of 4 V (anodic current: 30 mA) effectively stimulated astaxanthin biosynthesis, resulting in a 36.9% increase in astaxanthin content in treated cells compared to untreated cells. Proper regulation of bioelectrochemical parameters can improve astaxanthin production in H. pluvialis culture using electric treatment.
APPLIED SCIENCES-BASEL
(2021)
Article
Agriculture, Multidisciplinary
Yongni Shao, Jialing Liu, Zhi Zhu, Yutian Wang, Yiming Zhu, Yan Peng
Summary: This study investigates the feasibility of using terahertz spectroscopy for synchronous detection on metabolites concentration in microalgae production process. By capturing terahertz spectrum of stressed Haematococcus Pluvialis under nitrogen starvation, a correlation between metabolite contents and characteristic peak intensities was established, showing relatively accurate prediction of metabolite concentrations. A precise model combining partial least squares with intensities of interval characteristic peaks demonstrated high prediction correlation coefficients, low root-mean-square error of prediction, and validation of terahertz spectroscopy for monitoring metabolites concentration.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2021)
Article
Microbiology
Yun Hwan Park, Jaewon Park, Jeong Sik Choi, Hyun Soo Kim, Jong Soon Choi, Yoon-E Choi
Summary: This study investigated the effects of ultrasonic treatment on Haematococcus pluvialis (H. pluvialis). It was found that ultrasonic stimulation acted as a stress resource in H. pluvialis cells in the red cyst stage, resulting in additional production of the valuable compound astaxanthin. Furthermore, genes related to astaxanthin synthesis and cellular ROS levels were found to increase in response to ultrasonic stimulation, indicating its oxidative stimulus effect.
JOURNAL OF MICROBIOLOGY
(2023)
Article
Agricultural Engineering
Baobei Wang, Xueshan Pan, Fang Wang, Lulu Liu, Jing Jia
Summary: This study analyzed the biochemical compositions and proteomics of mixotrophic Haematococcus pluvialis under different light conditions. The findings showed that high light exposure increased astaxanthin, carbohydrate, and fatty acid contents, and regulated multiple metabolic pathways. A regulatory model for mixotrophic Haematococcus pluvialis coping with high light stress was proposed.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Jialu Wei, Xin Zhang, Sijie Ai, Yichen Huang, Xu Yang, Yan Mei, Kun Zhang, Haiying Wang
Summary: Immobilizing Haematococcus pluvialis in bacterial cellulose can improve astaxanthin content by up-regulating key genes associated with its synthesis. This novel cultivation approach shows promising results in increasing astaxanthin productivity compared to traditional suspension cultures.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Rachel Cray, Ira Levine
Summary: This study quantifies the relationship between astaxanthin and ROS in H. pluvialis, confirming the role of ROS as a modulator of carotenogenesis and astaxanthin as a protective mechanism against oxidative stress. The findings can be applied to optimize commercial astaxanthin production, reducing production time requirements by over 10%.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Chemistry, Medicinal
Kang Hyun Lee, Youngsang Chun, Ja Hyun Lee, Chulhwan Park, Hah Young Yoo, Ho Seok Kwak
Summary: This study presents an effective screening strategy using the negative phototaxis of Haematococcus pluvialis to obtain mutants with high astaxanthin production. A microfluidic device based on polydimethylsiloxane (PDMS) was developed to efficiently evaluate the phototactic response of H. pluvialis. Mutants sensitive to blue light were selected from a random mutant library based on their photosensitivity. The M1 strain obtained from this screening showed improved growth rate and astaxanthin production in a photo-bioreactor compared to the wild type.
Article
Food Science & Technology
Qingsheng Fan, Zhan Chen, Yating Wu, Jiangxin Zhu, Zhou Yu
Summary: The study demonstrates that astaxanthin significantly enhances immune functions in mice without causing toxic effects at experimental doses.
Article
Biochemistry & Molecular Biology
Oleksandr Virchenko, Tryggvi Stefansson
Summary: This study aimed to assess the effect of light on the spectrometric and high-performance liquid chromatography (HPLC) measurements of astaxanthin. The results showed that after 3 hours of light exposure, astaxanthin absorbance exhibited logarithmic growth of 8-11% (UV/Vis) and 7-17% (HPLC).