期刊
NUCLEIC ACID THERAPEUTICS
卷 22, 期 5, 页码 360-363出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/nat.2012.0352
关键词
-
资金
- Special Education and Research Expenses of the Ministry of Education, Culture, Sports, Science, and Technology of Japan
We previously reported that an octaarginine- and pH-sensitive fusogenic peptide-modified multifunctional envelope-type nano device (R8-GALA-MEND) produces a high level of gene expression in the liver. In this study, we report on an examination of whether this gene delivery system exerts potent hepatoprotective effects against lipopolysaccharide/D-galactosamine (LPS/D-GalN)-induced acute liver injury. In vivo-jetPEI (TM)-Gal, a commercially available in vivo transfection reagent, was used as a reference. The systemic administration of the R8-GALA-MEND or in vivo-jetPEI (TM)-Gal showed that the latter was more toxic than the R8-GALA-MEND, indicating that R8-GALA-MEND is a safer system than in vivo-jetPEI (TM)-Gal. Pretreatment with R8-GALA-MEND or in vivo-jetPEI (TM)-Gal loaded with hepatocyte growth factor (HGF) pDNA inhibited serum GPT and GOT levels from becoming elevated. However, the survival rate of the mice was significantly enhanced in the case of R8-GALA-MEND, but not for the in vivo-jetPEI (TM)-Gal treatment. These results demonstrate that R8-GALA-MEND has the potential for use in the pretreatment of an acute liver injury.
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