Review
Biochemistry & Molecular Biology
Pengxu Wei, Ruixue Bao
Summary: Knee osteoarthritis is a common problem, especially in older individuals, and can eventually lead to disability. Injecting mesenchymal stem cells (MSCs) into the knee joint has been shown to induce cartilage regeneration in several studies. Additionally, understanding the anatomical, biomechanical, and biochemical changes associated with knee osteoarthritis can help develop appropriate interventions to delay disease progression and improve cartilage repair induced by MSC injection. Post-injection rehabilitation programs and related mechanisms are also discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Diego de Carvalho Carneiro, Lila Teixeira de Araujo, Girlaine Cafe Santos, Patricia Kauanna Fonseca Damasceno, Jaqueline Leite Vieira, Ricardo Ribeiro dos Santos, Josiane Dantas Viana Barbosa, Milena Botelho Pereira Soares
Summary: Osteoarthritis is a joint disease characterized by cartilage deterioration. Intra-articular injections of mesenchymal stem cells have shown promising results in animal models and humans. However, further clinical trials are needed to support the efficacy and safety of this approach in the regeneration of articular cartilage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, Research & Experimental
Jiadong Li, Hao Zhang, Yafei Han, Yan Hu, Zhen Geng, Jiacan Su
Summary: Osteoarthritis (OA) is a degenerative disease characterized by loss of articular cartilage and chronic inflammation, leading to multiple dysfunctions and tissue lesions. However, drug delivery to the affected joints is hindered by non-vascular environment and dense cartilage matrix, resulting in low drug bioavailability. Biomaterials have shown promise in improving drug targeting and achieving precision therapy for OA. This article reviews the understanding of OA's pathological mechanisms, discusses advances in different types of targeted biomaterials, and analyzes limitations and challenges in clinical translation and biosafety, aiming to guide future therapeutic strategies for OA.
Review
Biotechnology & Applied Microbiology
Xia Zhao, Daniel A. Hu, Di Wu, Fang He, Hao Wang, Linjuan Huang, Deyao Shi, Qing Liu, Na Ni, Mikhail Pakvasa, Yongtao Zhang, Kai Fu, Kevin H. Qin, Alexander J. Li, Ofir Hagag, Eric J. Wang, Maya Sabharwal, William Wagstaff, Russell R. Reid, Michael J. Lee, Jennifer Moriatis Wolf, Mostafa El Dafrawy, Kelly Hynes, Jason Strelzow, Sherwin H. Ho, Tong-Chuan He, Aravind Athiviraham
Summary: Cartilage, especially articular cartilage, is crucial for joint function and mobility, but has limited self-repair capacity. Cartilage tissue engineering (CTE) holds promise for repair, requiring effective chondrogenic factors, ample chondrogenic progenitors, and cell-friendly, biocompatible scaffold materials for success. Progress in these areas, along with advancements in 3D bio-printing, may improve clinical outcomes of cartilage repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Cell Biology
Timothy P. Liu, Pin Ha, Crystal Y. Xiao, Sang Yub Kim, Andrew R. Jensen, Jeremiah Easley, Qingqiang Yao, Xinli Zhang
Summary: There is a need for novel therapeutics for regenerating injured articular cartilage. Mesenchymal stem cells (MSCs) show promise in chondrogenic differentiation and healing, but further investigation in large animal models is needed. Large animal models play a critical role in studying human MSCs in an osteochondral microenvironment and identifying promising approaches for clinical investigation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Parviz Vahedi, Rana Moghaddamshahabi, Thomas J. Webster, Ayse Ceren Calikoglu Koyuncu, Elham Ahmadian, Wasim S. Khan, Ali Jimale Mohamed, Aziz Eftekhari
Summary: Cartilage damage is often difficult to repair, and current treatment strategies are inadequate. Autologous mesenchymal stem cells are considered a promising therapeutic approach for cartilage regeneration, as they possess robust cartilaginous production capacity and are easy to isolate and expand without causing morbidity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Manon Jammes, Romain Contentin, Fabrice Audigie, Frederic Casse, Philippe Galera
Summary: This study tested the effects of different storage conditions on the secretome of mesenchymal stromal cells (MSCs) and investigated the enhancement of MSC secretome properties through cytokine priming. The results showed that secretome stored at -80°C, -20°C, and 4°C improved collagen protein accumulation, cell proliferation, and inflammation reduction in chondrocytes. Cytokine priming had different effects on the expression of immunomodulatory molecules and influenced the behavior of equine articular chondrocytes (eACs). These findings demonstrate the potential of MSC-conditioned media for improving the management of equine osteoarthritis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Marco Caforio, Carmelo Nobile
Summary: The study found that the intra-articular administration of autologous purified adipose tissue following arthroscopy significantly reduces pain and improves functional recovery in patients with knee osteoarthritis. No adverse effects were observed during the 1-year follow-up.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Engineering, Biomedical
Lang Bai, Qibin Han, Zeyu Han, Xiaoyu Zhang, Jingwen Zhao, Huitong Ruan, Junliang Wang, Feng Lin, Wenguo Cui, Xing Yang, Yuefeng Hao
Summary: By utilizing chitosan-based cell adhesion, a bioadhesive porous microsphere is developed as a cell carrier for accelerating articular cartilage regeneration. After injection into the joint cavity, the microspheres expedite the release of mesenchymal stem cells (MSCs) in the microenvironment of arthritis inflammation, while using kartogenin to promote the differentiation of MSCs into chondrocytes, thereby accelerating articular cartilage regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Jay M. Patel, Claudia Loebel, Kamiel S. Saleh, Brian C. Wise, Edward D. Bonnevie, Liane M. Miller, James L. Carey, Jason A. Burdick, Robert L. Mauck
Summary: This study demonstrates that a hyaluronic acid hydrogel system can interdigitate with and promote sealing of degenerated cartilage, restoring fluid pressurization within the tissue and preventing further fluid flow and matrix loss from the defect surface. Additionally, the hydrogel therapy can guide localized extracellular matrix deposition through presenting chemo mechanical cues, showing therapeutic potential for damaged cartilage treatment across different length scales.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Nanoscience & Nanotechnology
Xu Li, Bingyang Dai, Jiaxin Guo, Lizhen Zheng, Quanyi Guo, Jiang Peng, Jiankun Xu, Ling Qin
Summary: The review discusses the pathological features of osteoarthritis, the design concept for accurate drug localization within cartilage, interactions of nanoparticles with cartilage microenvironment, and advances in therapeutic schemes. The emphasis is on developing smart bioresponsive and multiple modality nanoparticles to interact with pathological signals.
NANO-MICRO LETTERS
(2021)
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Jin Qiu, Bingqiang Hua, Xiaoping Ye, Xianwen Liu
Summary: Kartogenin (KGN) is a small-molecule compound that promotes chondrogenic differentiation of mesenchymal stem cells and alleviates knee joint osteoarthritis. Its effect on temporomandibular joint osteoarthritis (TMJOA) remains unclear.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Health Care Sciences & Services
Yang Yu, Qunshan Lu, Songlin Li, Mingxing Liu, Houyi Sun, Lei Li, Kaifei Han, Peilai Liu
Summary: This study examines the efficacy and safety of autologous micro-fragmented adipose tissue (MF-AT) in improving joint function and cartilage repair in knee osteoarthritis patients. The results show improvements in WOMAC, VAS, KSS, and HSS scores at 3, 6, 9, 12, and 18 months after injection compared to before injection. There were no adverse events reported. However, the improved knee function was not sustained, with the best results occurring at 9-12 months post-injection.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cell Biology
Liang Lu, Xifu Shang, Bin Liu, Weijian Chen, Yu Zhang, Shuyun Liu, Xiang Sui, Aiyuan Wang, Quanyi Guo
Summary: The study demonstrated that the cartilage complex constructed by ADSCs and ACECM through tissue engineering successfully repaired cartilage defects, indicating the potential of ADSCs for chondrogenic differentiation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Orthopedics
Maik Hoberg, Christian Konrads, Simon Huber, Stephan Reppenhagen, Matthias Walcher, Andre Steinert, Thomas Barthel, Maximilian Rudert
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
(2015)
Article
Orthopedics
Maik Hoberg, Christian Konrads, Jana Engelien, Dorothee Oschmann, Michael Holder, Matthias Walcher, Andre Steinert, Maximilian Rudert
INTERNATIONAL ORTHOPAEDICS
(2016)
Article
Orthopedics
S. Reppenhagen, F. Gilbert, C. Konrads, S. Goebel, A. Steinert, M. Hoberg, C. Loeffler, M. Rudert, T. Barthel
OPERATIVE ORTHOPADIE UND TRAUMATOLOGIE
(2015)
Article
Orthopedics
A. F. Steinert, L. Sefrin, M. Hoberg, J. Arnholdt, M. Rudert
Article
Orthopedics
J. T. Sieker, M. Kunz, M. Weissenberger, F. Gilbert, S. Frey, M. Rudert, A. F. Steinert
OSTEOARTHRITIS AND CARTILAGE
(2015)
Article
Cell & Tissue Engineering
Regina Ebert, Peggy Benisch, Melanie Krug, Sabine Zeck, Jutta Meissner-Weigl, Andre Steinert, Martina Rauner, Lorenz Hofbauer, Franz Jakob
STEM CELL RESEARCH
(2015)
Article
Cell & Tissue Engineering
Andre F. Steinert, Manuela Kunz, Patrick Prager, Sascha Goebel, Ludger Klein-Hitpass, Regina Ebert, Ulrich Noeth, Franz Jakob, Frank Gohlke
STEM CELL RESEARCH & THERAPY
(2015)
Article
Anesthesiology
Sascha Goebel, Andre Steinert, Christina Vierheilig, Hermann Faller
CLINICAL JOURNAL OF PAIN
(2013)
Article
Biotechnology & Applied Microbiology
Conrad Weber, Nicole Armbruster, Carsten Scheller, Florian Kreppel, Stefan Kochanek, Axel Rethwilm, Andre F. Steinert
JOURNAL OF GENE MEDICINE
(2013)
Article
Biotechnology & Applied Microbiology
Nicole Armbruster, Conrad Weber, Tatiana Wictorowicz, Axel Rethwilm, Carsten Scheller, Andre F. Steinert
JOURNAL OF GENE MEDICINE
(2014)
Article
Orthopedics
P. Niemeyer, S. Andereya, P. Angele, A. Ateschrang, M. Aurich, M. Baumann, P. Behrens, U. Bosch, C. Erggelet, S. Fickert, J. Fritz, H. Gebhard, K. Gelse, D. Guenther, A. Hoburg, P. Kasten, T. Kolombe, H. Madry, S. Marlovits, N. M. Meenen, P. E. Mueller, U. Noeth, J. P. Petersen, M. Pietschmann, W. Richter, B. Rolauffs, K. Rhunau, B. Schewe, A. Steinert, M. R. Steinwachs, G. H. Welsch, W. Zinser, D. Albrecht
ZEITSCHRIFT FUR ORTHOPADIE UND UNFALLCHIRURGIE
(2013)
Article
Multidisciplinary Sciences
Jakob T. Sieker, Maximilian Rudert, Andre F. Steinert
Article
Orthopedics
Malin Meier, Sumesh Zingde, Raymond Best, Lennart Schroeder, Johannes Beckmann, Andre Friedrich Steinert
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2020)
Article
Chemistry, Multidisciplinary
Mike Wagenbrenner, Konrad Poker, Tizian Heinz, Marietta Herrmann, Konstantin Horas, Regina Ebert, Susanne Mayer-Wagner, Boris M. Holzapfel, Maximilian Rudert, Andre F. Steinert, Manuel Weissenberger
Summary: This study compared the in vitro chondrogenic potential of MSC-like cells from different tissue sources and found that regardless of the cell source, all MSC-like cells showed multipotent differentiation potential. This provides insight into the suitability of various tissue origins for cell-based cartilage therapies and tissue engineering, leading to increased flexibility in cell availability.
APPLIED SCIENCES-BASEL
(2022)
Article
Surgery
K. Fehske, A. Steinert, H. -J. Eichhorn