Review
Cell & Tissue Engineering
Zeinab Shirbaghaee, Mohammad Hassani, Saeed Heidari Keshel, Masoud Soleimani
Summary: This article reviews the immunomodulatory features of mesenchymal stem cells (MSCs) and their potential applications as therapeutic agents in the treatment of critical limb ischemia (CLI). Despite advances in clinical research, MSC therapy is still considered experimental and not recommended for regular clinical use. The article provides recommendations to improve the efficacy of cell therapy for CLI patients.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Marta Rojas-Torres, Ma Carmen Duran-Ruiz
Summary: Critical limb ischemia is the most severe form of peripheral arterial disease, commonly caused by atherosclerosis leading to progressive blockage of arterial vessels. For patients not suitable for surgical intervention, angiogenic cell therapy has emerged as a promising alternative, showing potential for enhancing revascularization by promoting vessel formation and blood flow recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Alessia Scatena, Pasquale Petruzzi, Filippo Maioli, Francesca Lucaroni, Cristina Ambrosone, Giorgio Ventoruzzo, Francesco Liistro, Danilo Tacconi, Marianna Di Filippi, Nico Attempati, Leonardo Palombi, Leonardo Ercolini, Leonardo Bolognese
Summary: The study showed that treatment with autologous PBMNC implants in NO-CLI patients with DFUs significantly reduced amputation rate, improved survival, and promoted wound healing. Autologous PBMNC injection may effectively prevent amputations in patients with DFUs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Pegah Nammian, Seyedeh-Leili Asadi-Yousefabad, Sajad Daneshi, Mohammad Hasan Sheikhha, Seyed Mohammad Bagher Tabei, Vahid Razban
Summary: The study compared the effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) in the treatment of critical limb ischemia (CLI). The results indicated that BM-MSCs had a stronger effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared to AT-MSCs. This suggests that BM-MSC transplantation could be a more effective therapy for diseases with insufficient angiogenesis, such as hindlimb ischemia.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Elvira V. Sobolevskaya, Oleg A. Shumkov, Mikhail A. Smagin, Andrey E. Guskov, Alexandra V. Malysheva, Victor V. Atuchin, Vadim V. Nimaev
Summary: Chronic obliterating lesions of the arteries of lower extremities, caused by atherosclerosis, lead to chronic ischemia and increase the risk of limb loss and cardiovascular mortality. Percutaneous transluminal balloon angioplasty is a minimally invasive approach for limb revascularization, but restenosis is still possible. Early detection of molecular changes as markers of restenosis can help identify at-risk patients and find ways to inhibit this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Peripheral Vascular Disease
Michal Dubsky, Vladimira Fejfarova, Robert Bem, Alexandra Jirkovska, Andrea Nemcova, Karol Sutoris, Jitka Husakova, Jelena Skibova, Edward B. Jude
Summary: The study evaluated the factors associated with nonresponse to ACT in diabetic patients with NO-CLI, revealing that inherited thrombotic disorders, particularly Leiden mutation and MTHFR 677 mutation, were significant predictors of impaired response to the treatment.
Review
Medicine, Research & Experimental
Alessandra Magenta, Maria Cristina Florio, Massimo Ruggeri, Sergio Furgiuele
Summary: Cell therapy is becoming an attractive alternative for patients with critical limb ischemia who are not suitable for surgery, with benefits including therapeutic angiogenesis and neovascularization to increase blood flow and promote tissue regeneration. The study highlights the negative effects of diabetes on different sources of autologous cells used in cell therapy, as well as delays in wound healing capacity. Additionally, relevant clinical trials and challenges arising from cell therapy trials are discussed, along with the introduction of cell therapy as an adjuvant therapy for promoting wound healing in revascularized diabetic patients.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Surgery
Joel L. Ramirez, Greg J. Zahner, Shipra Arya, S. Marlene Grenon, Warren J. Gasper, Julie Ann Sosa, Michael S. Conte, James C. Iannuzzi
Summary: This study identified an association between depression and nonhome discharge (NHD) after revascularization for CLTI. The presence of depression was linked to an increased risk of NHD, especially in endovascular revascularization cases. There was a higher odds of NHD in men with depression compared to women with depression, suggesting a gender difference in this association.
JOURNAL OF VASCULAR SURGERY
(2021)
Article
Peripheral Vascular Disease
Zoe S. Terwilliger, Terence E. Ryan, Emma J. Goldberg, Cameron A. Schmidt, Dean J. Yamaguchi, Reema Karnekar, Patricia Brophy, Thomas D. Green, Tonya N. Zeczycki, Feilim Mac Gabhann, Brian H. Annex, Joseph M. McClung
Summary: CLI is the most severe manifestation of PAD with higher prevalence in Black patients. Differences in bioenergetic paradigm and alterations in specific pathways were observed in the limb muscles of Black individuals with CLI.
Article
Cell Biology
Yipeng Du, Xiaoting Li, Wenying Yan, Zhaohua Zeng, Dunzheng Han, Hong Ouyang, Xiudi Pan, Bihui Luo, Bohua Zhou, Qiang Fu, Dongfeng Lu, Zheng Huang, Zhiliang Li
Summary: The study utilized mutant MetRS to label proteomes of mesenchymal stem cells and demonstrated efficient incorporation of ANL in protein synthesis in ischemic hind limb. The transplanted MSCs significantly improved blood reperfusion and vessel density while reducing inflammation in hindlimb ischemia model. Proteomic analysis revealed differential protein expression in apoptosis and energy metabolism pathways between ischemic and sham groups.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Lucas Grove Vejlstrup Bager, Jeppe Kofoed Petersen, Eva Havers-Borgersen, Timothy Resch, Kim G. Smolderen, Carlos Mena-Hurtado, Jonas Eiberg, Lars Kober, Emil Loldrup Fosbol
Summary: This study aimed to describe the practice patterns of evidence-based medical therapy (EBM) and overall mortality in high-risk patients with critical limb-threatening ischaemia (CLTI), compared with patients with myocardial infarction (MI). The results showed that, despite similar treatment guidelines, patients with CLTI were undertreated with EBM and had a higher risk of death, compared with patients with MI.
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2023)
Article
Cell & Tissue Engineering
Zeinab Shirbaghaee, Saeed Heidari Keshel, Mehdi Rasouli, Majid Valizadeh, Seyed Saeed Hashemi Nazari, Mohammad Hassani, Masoud Soleimani
Summary: This study aimed to evaluate the feasibility of using placenta-derived mesenchymal stem cells (P-MSCs) for the treatment of CLI patients. The results showed that P-MSCs treatment was safe and well tolerated and could significantly improve physical performance and minimize inflammatory conditions in patients with CLI.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Ayman Elbadawi, Kirolos Barssoum, Michael Megaly, Devesh Rai, Ahmed Elsherbeeny, Hend Mansoor, Mehdi H. Shishehbor, Ahmed Abdel-Latif, Martha Gulati, Islam Y. Elgendy
Summary: In a nationwide analysis of CLI hospitalizations, women were older and less likely to undergo revascularization. Women had higher in-hospital mortality and bleeding complications compared to men. Sex-specific interventions are needed to reduce these disparities in this high-risk population.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Medicine, General & Internal
Yuta Suto, Wakana Sato, Takayuki Yamanaka, Mayu Unuma, Yuki Kobayashi, Mako Aokawa, Hiroyuki Watanabe
Summary: This study investigated the utility of superb microvascular imaging (SMI) for assessing foot perfusion in critical limb ischemia (CLI) patients. The results demonstrated that SMI-VI had high diagnostic performance for CLI and could visualize and quantify foot microcirculation based on the angiosome. This study suggests that SMI is a useful tool for assessing foot perfusion in CLI patients.
Article
Cardiac & Cardiovascular Systems
Andrea Panunzi, Fabiana Madotto, Elena Sangalli, Federica Riccio, Adriana Barbara Sganzaroli, Paolo Galenda, Amelia Bertulessi, Maria Francesca Barmina, Ornella Ludovico, Orazio Fortunato, Francesco Setacci, Flavio Airoldi, Davide Tavano, Laura Giurato, Marco Meloni, Luigi Uccioli, Antonino Bruno, Gaia Spinetti, Carlo Maria Ferdinando Caravaggi
Summary: This study observed the effects of PB-MNCs therapy in no-option CLTI diabetic patients. The results showed improved tissue perfusion, high rate of ulcer healing, and restoration of walking capability. Evaluating the frequency of circulating angiogenic cells and extracellular vesicles could help identify patients with better long-term clinical benefits.
CARDIOVASCULAR DIABETOLOGY
(2022)
Article
Transplantation
Luca De Nicola, Raffaele Serra, Michele Provenzano, Roberto Minutolo, Ashour Michael, Nicola Ielapi, Stefano Federico, Rosa Carrano, Vincenzo Bellizzi, Carlo Garofalo, Carmela Iodice, Silvio Borrelli, Giuseppe Grandaliano, Giovanni Stallone, Loreto Gesualdo, Paolo Chiodini, Michele Andreucci
Summary: This study compared the risk and determinants of end-stage kidney disease (ESKD) between kidney transplant recipients (KTR) and patients with native chronic kidney disease (CKD). The results showed that the risk of ESKD in KTR was lower in the first 5 years and higher later on compared to CKD patients. Traditional determinants of ESKD accounted for one-third of the variability of time-to-graft failure.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2023)
Article
Surgery
Marianna Sallustro, Antonio Peluso, Davide Turchino, Isidoro Maione, Flavia Vita, Eugenio Martelli, Raffaele Serra, Umberto Marcello Bracale
Summary: This study aimed to evaluate the safety and effectiveness of a new generation drug-coated balloon (DCB) for the treatment of femoropopliteal lesions. The results showed that patients treated with the DCB exhibited lower restenosis/reocclusion rate and improved primary patency compared to those treated with plain old balloon angioplasty (POBA).
ANNALS OF VASCULAR SURGERY
(2023)
Review
Biochemistry & Molecular Biology
Davide Costa, Michele Andreucci, Nicola Ielapi, Giuseppe Filiberto Serraino, Pasquale Mastroroberto, Umberto Marcello Bracale, Raffaele Serra
Summary: Chronic Venous Disease (CVD) is a complex and multifactorial condition involving genetic, proteomic, and cellular mechanisms that result in structural and functional changes to the veins of lower limbs. The expression of certain genes and alterations in the extracellular matrix play a role in the susceptibility to CVD. This article provides an up-to-date overview of the molecular determinants of CVD, including genetic and hormonal influences, ECM imbalance, histopathology, and the role of endothelial dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Giuseppe Filiberto Serraino, Federica Jiritano, Davide Costa, Nicola Ielapi, Domenica Battaglia, Umberto Marcello Bracale, Pasquale Mastroroberto, Michele Andreucci, Raffaele Serra
Summary: This systematic review aims to explore the role of matrix metalloproteinases (MMPs) in routine cardiac operations, especially cardiopulmonary bypass (CPB). The results show that MMP levels are significantly correlated with CPB and the gelatinases MMP-2 and MMP-9 are highly released during cardiac surgery with CPB. Increased MMP levels are associated with adverse post-operative outcomes. Future large-scale prospective multicenter randomized clinical trials are needed to investigate the impact of mechanical and pharmacological strategies on the inflammatory response and postoperative outcomes in cardiac surgery and CPB.
Review
Surgery
Davide Costa, Michele Andreucci, Nicola Ielapi, Giuseppe Filiberto Serraino, Pasquale Mastroroberto, Umberto Marcello Bracale, Raffaele Serra
Summary: This review examines the biomolecular and cellular processes that contribute to the formation and progression of arterial aneurysms, with a focus on vascular remodeling and the role of genetic alterations and extracellular matrix imbalance.
ANNALS OF VASCULAR SURGERY
(2023)
Article
Cardiac & Cardiovascular Systems
Roberto Minici, Raffaele Serra, Nicola De Rosi, Salvatore Ciranni, Marisa Talarico, Maria Petulla, Giuseppe Guzzardi, Federico Fontana, Domenico Lagana
Summary: This study analyzed the feasibility, safety, and efficacy of retrograde crossing of femoro-popliteal occlusions with tibial access. Results showed that this approach is a viable, effective, and safe alternative to the failed antegrade approach.
CATHETERIZATION AND CARDIOVASCULAR INTERVENTIONS
(2023)
Article
Biochemistry & Molecular Biology
Antonella La Russa, Danilo Lofaro, Alberto Montesanto, Daniele La Russa, Gianluigi Zaza, Simona Granata, Michele Di Dio, Raffaele Serra, Michele Andreucci, Renzo Bonofiglio, Anna Perri
Summary: In this study, the association of functional variants of NLRP3 pathway-related genes (NLRP3-rs10754558, CARD8-rs2043211) with CKD susceptibility was investigated for the first time. Logistic regression analyses revealed significantly higher frequencies of the G allele of NLRP3 variant (67.3%) and the T allele of CARD8 variant (70.8%) among cases compared to controls (35.9% and 31.2%, respectively). The results suggest that NLRP3 rs10754558 and CARD8 rs2043211 variants may be associated with susceptibility to CKD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Health Care Sciences & Services
Roberto Minici, Raffaele Serra, Claudio Maglia, Giuseppe Guzzardi, Marco Spinetta, Federico Fontana, Massimo Venturini, Domenico Lagana
Summary: This study evaluated the efficacy and safety of the new chitosan-based hemostatic dressing, Axiostat (R), in assisting manual compression closure of the femoral arterial access site in patients undergoing endovascular treatments, and compared the outcomes with manual compression alone and vascular closure devices. The results showed that Axiostat (R) was effective and safe in achieving hemostasis of the femoral arterial access site in patients undergoing endovascular treatment with a 4-8 Fr introducer sheath.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Social Sciences, Interdisciplinary
Davide Costa, Nicola Ielapi, Egidio Bevacqua, Salvatore Ciranni, Lucia Cristodoro, Giuseppina Torcia, Raffaele Serra
Summary: Several factors, known as social determinants of health (SDHs), including gender, SES, and ethnicity, contribute to health inequality. This study analyzed inequality in vascular diseases (VDs) such as PAD, CVD, AAA, and CS. Through a systematic review, the study provided a comprehensive overview of the evidence on SDHs in VDs. Findings showed that gender, SES, and ethnicity play important roles in VDs, highlighting specific issues in this context. A model based on action items for SDHs associated with VDs was proposed.
SOCIAL SCIENCES-BASEL
(2023)
Review
Materials Science, Biomaterials
Davide Costa, Michele Andreucci, Nicola Ielapi, Giuseppe Filiberto Serraino, Pasquale Mastroroberto, Umberto Marcello Bracale, Raffaele Serra
Summary: This review provides the most updated evidence on the pathogenesis, prevention, diagnosis, and treatment of vascular graft or endograft infection. Multiple microorganisms can cause infections, with Staphylococcus aureus being the most common. Diagnosis requires a combination of clinical, surgical, radiologic, and laboratory criteria. Surgery and antimicrobial therapy are the main treatments, with alternative options available for patients who cannot undergo surgery.
Article
Medicine, General & Internal
Davide Costa, Nicola Ielapi, Roberto Minici, Antonio Peluso, Umberto Marcello Bracale, Michele Andreucci, Raffaele Serra
Summary: This study aims to evaluate risk factors for bleeding varicose veins (VVs) in patients with chronic venous disease (CVD). The findings suggest that advanced clinical stage of CVD, advanced age, living alone, cardiovascular comorbidities, certain drugs affecting blood coagulation, psychotropic medication, specific venous reflux patterns, and lack of previous assessment and treatment for CVD may predispose patients to a high risk of bleeding VVs.
MEDICINA-LITHUANIA
(2023)
Article
Social Sciences, Interdisciplinary
Davide Costa, Raffaele Serra
Summary: This study is the first to explore the dietary regime in a total institution and focuses on the gender differences in mental health patients' diets. Using archival and documentary evidence, the study examines the diets of mental health patients in Girifalco asylum during the 19th century. The evidence analyzed explores whether these dietary regimes align with the principles of moral treatment.
SOCIAL SCIENCES-BASEL
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Roberto Minici, Michele Mercurio, Giuseppe Guzzardi, Massimo Venturini, Federico Fontana, Luca Brunese, Pasquale Guerriero, Raffaele Serra, Filippo Piacentino, Marco Spinetta, Lorenzo Zappia, Davide Costa, Andrea Coppola, Stephan MGJR Res Team, Olimpio Galasso, Domenico Lagana
Summary: This study compares the outcomes of angioembolization for the treatment of pelvic traumatic arterial injury in patients with hemodynamic stability vs. hemodynamic instability. The results show that angioembolization is an effective and safe option for managing traumatic pelvic arterial lesions, even in hemodynamically unstable patients.
Article
Medicine, General & Internal
Roberto Minici, Pasquale Guerriero, Federico Fontana, Massimo Venturini, Giuseppe Guzzardi, Filippo Piacentino, Andrea Coppola, Marco Spinetta, Agostino Siciliano, Raffaele Serra, Davide Costa, Nicola Ielapi, Rita Santoro, Domenico MGJR Res Team, Luca Brunese, Domenico Lagana
Summary: This multicenter study evaluated the effectiveness and safety of endovascular treatment of visceral pseudoaneurysms using NALEAs. The results showed that both ruptured and unruptured VAPs can be effectively and safely treated with NALEA-based endovascular embolization. The use of NALEAs is especially recommended in specific clinical cases with coagulopathy, fragile vessels, and challenging embolization targets.
MEDICINA-LITHUANIA
(2023)
Article
Medicine, General & Internal
Cristina Vocca, Gianmarco Marciano, Vincenzo Rania, Luca Catarisano, Caterina Palleria, Salvatore Ciranni, Giuseppina Torcia, Raffaele Serra, Francesco Monea, Giuseppe Spaziano, Giovambattista De Sarro, Rita Citraro, Luca Gallelli
Summary: KTS is a rare genetic disease characterized by venous and capillary malformations and lymphatic malformation. This article describes a case where a severe skin wound in a KTS patient was unresponsive to common treatment but showed improvement with a combination therapy of oxygen-ozone therapy, pulsed magnetic fields, and topical medication. This suggests a potential multistep approach for treating rare and severe diseases.
