Review
Chemistry, Multidisciplinary
Eleonora Macchia, Fabrizio Torricelli, Paolo Bollella, Lucia Sarcina, Angelo Tricase, Cinzia Di Franco, Ronald Osterbacka, Zsolt M. Kovacs-Vajna, Gaetano Scamarcio, Luisa Torsi
Summary: Nanometric bioelectronic transducing surfaces have been the main method for detecting single molecules, but are unsuitable for assays at concentrations below the nanomolar level. However, some large-area transistors have shown detection limits lower than femtomolar.
Article
Chemistry, Analytical
Qi Wang, Huaqian Xin, Zhou Wang
Summary: A label-free immunosensor for detecting carcinoembryonic antigen (CEA) was constructed with high electron conductivity and excellent antibody fixation ability, demonstrating balanced performance with a wide linear range, low detection limit, and satisfactory selectivity, repeatability, and stability.
Article
Chemistry, Analytical
Dexin Du, Jue Wang, Mingquan Guo, Jiangnan Shu, Wei Nie, Zhiping Bian, Di Yang, Hua Cui
Summary: Label-free electrochemiluminescence (ECL) immunoassays (lf-ECLIA) have become an attractive method for biomarker detection due to their simplicity, speed, and cost-effectiveness. This study investigated the interaction between biomarkers and analytical interfaces constructed using a nanoluminophore and antibody-functionalized gold nanoparticles. The results showed that positively charged biomarkers increased the ECL intensity, while negatively charged biomarkers decreased the ECL intensity. A charge-dependent lf-ECLIA method was established based on the ECL signal changes, which exhibited different responses to positively charged and negatively charged biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Francisco Martinez-Rojas, Erik Castaneda, Francisco Armijo
Summary: A label-free electrochemical immunosensor was developed for the detection of PSA using specific antibody immobilization, which showed excellent stability and sensitivity. The calibration curve displayed a linear range and low limits of detection and quantification, indicating the potential for clinical detection of PSA concentrations.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Peng Huang, Lingzhang Meng, Jun Pang, Haiting Huang, Jing Ma, Linlin He, Parnian Amani
Summary: This study successfully developed a label-free electrochemical immunosensor based on a metal-organic framework (MOF)-graphene oxide nanocomposite for the detection of carcinoembryonic antigen (CEA), a tumor marker in lung cancer for early cancer diagnosis. The immunosensor showed improved sensitivity, stability, and selectivity, and demonstrated satisfactory accuracy in detecting CEA in human serum samples. These findings suggest that the prepared CEA immunosensor has potential for clinical applications and analysis of human fluids.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Linlin Cao, Wen Zhang, Sumei Lu, Chengjie Guo, Peijun Wang, Dantong Zhang, Wanshan Ma
Summary: In this study, a snowflake Cu2S/Pd/CuO nanocomposite was developed to construct an original label-free electrochemical immunosensor for ultrasensitive detection of CEA levels. The immunosensor showed excellent detection performance and potential value for clinical application in detecting human serum samples.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Xing-Pei Liu, Jing-Shuai Chen, Chang-Jie Mao, Bao-Kang Jin
Summary: A label-free photoelectrochemical immunosensor based on a g-C3N4/CdSe nanocomposite was successfully established for detecting carcinoembryonic antigen (CEA). The sensor showed excellent selectivity and repeatability for CEA detection with a detection limit of 0.21 ng mL(-1) and the potential to detect other protein targets.
Article
Biochemical Research Methods
Maryam Jozghorbani, Mojtaba Fathi, Sayed Habib Kazemi, Navid Alinejadian
Summary: A label-free electrochemical immunosensor for detecting CEA was developed, which showed promising results for clinical screening through studying the electrochemical behavior of antibody-antigen immune complexes.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Minghua Deng, Jinhua Li, Bichen Xiao, Zhanpeng Ren, Ziqin Li, Haiyang Yu, Jiashen Li, Jianying Wang, Zhaowei Chen, Xianbao Wang
Summary: In this study, highly sensitive label-free DNA biosensors were developed using carbon quantum dot-functionalized solution-gated graphene transistors, which showed a low detection limit, wide linear range, and good specificity. The sensor can effectively distinguish one-base mismatched target DNA, providing promising perspectives for biosensor applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Malik Saddam Khan, Hafsa Ameer, Yuwu Chi
Summary: A novel and simple label-free electrochemiluminescence immunosensor was proposed using Ce2Sn2O7 nanocubes as ECL emitters, which were functionalized with gold nanoparticles to enhance ECL emission. The immunosensor showed improved ECL performance for detecting CEA concentrations, with outstanding stability, reproducibility, and specificity, making it an innovative method for sensitive analysis of biomarkers in real samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Electrical & Electronic
Dongchen Zhu, Grant A. Cathcart, Satoshi Ihida, Hiroshi Toshiyoshi, Agnes Tixier-Mita, Yasuyuki Sakai, Kikuo Komori
Summary: A label-free immunosensor using TFT-mu EAs with a large sensing area and transparent ITO electrodes was developed, successfully detecting HSA by recording changes in electric double layer capacitance. Optimization of ionic strength and pH value resulted in a linear range of 0.3-300 pM for HSA detection. This technology allows for label-free multiple immunosensing by patterning antibodies on its surface.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2021)
Article
Biophysics
Sian-Hong Ciou, Ao-Ho Hsieh, Yu-Xiu Lin, Jhao-Liang Sei, Mani Govindasamy, Chang-Fu Kuo, Chi-Hsien Huang
Summary: Alzheimer's disease (AD) can be diagnosed using advanced imaging, but recent research suggests early screening using biomarkers in peripheral blood is feasible. Plasma tau proteins phosphorylated at specific sites, such as p-tau217, show promising potential as biomarkers. However, a biosensor with high sensitivity and specificity for detecting p-tau217 has not been reported. In this study, a label-free solution-gated field effect transistor (SGFET)-based biosensor was developed, which showed good linear electrical response to p-tau217 protein concentrations in the range of 10 fg/ml to 100 pg/ml. The biosensor exhibited high sensitivity and specificity, as well as stability.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Gulam Rabbani, Mohammad Ehtisham Khan, Ejaz Ahmad, Mohsin Vahid Khan, Abrar Ahmad, Anwar Ulla Khan, Wahid Ali, Mazin A. Zamzami, Abdullateef H. Bashiri, Waleed Zakri
Summary: C-reactive protein (CRP) is a biomarker that can be used for the accurate diagnosis of cardiovascular risk, type-2 diabetes, metabolic syndrome, hypertension, and various types of cancers. This study successfully developed a highly sensitive and selective carbon nanotube field-effect transistor (CNT-FET) immunosensor for detecting CRP, with a wide dynamic range, rapid response time, and low variation. The use of this immunosensor can provide a low-cost and rapid clinical detection technology for the early diagnosis of coronary heart disease (CHD) and replace traditional expensive laboratory-based procedures.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Akanksha Roberts, Subhasis Mahari, Deepshikha Shahdeo, Sonu Gandhi
Summary: A novel immunosensor for detecting the spike protein of the coronavirus was reported, showing high sensitivity and stability under optimized conditions for detecting very low levels of the virus protein in standard buffer. This immunosensor could serve as a rapid, specific, and sensitive diagnostic tool for detecting traces of the coronavirus spike antigen in clinical samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Hedyeh Rahmani, Samira Mansouri Majd, Abdollah Salimi, Foad Ghasemi
Summary: Measuring CA 19-9 antigen level plays a critical role in early diagnosis, treatment monitoring, and disease recurrence prediction of pancreatic cancer. This research assesses the application of few-layered TiS3 nanoribbons as a channel material in an immunosensor for rapid detection of CA 19-9 antigen. The results show the potential of the developed immunosensor for cancer diagnosis and therapeutic monitoring.
Article
Chemistry, Analytical
Sho Hideshima, Mai Saito, Keisuke Fujita, Yoshitaka Harada, Mika Tsuna, Satoshi Sekiguchi, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Instruments & Instrumentation
Ke Si, Shanshan Cheng, Sho Hideshima, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka
SENSORS AND MATERIALS
(2018)
Article
Multidisciplinary Sciences
Sho Hideshima, Hiroki Hayashi, Hiroshi Hinou, Shunsuke Nambuya, Shigeki Kuroiwa, Takuya Nakanishi, Toshiyuki Momma, Shin-Ichiro Nishimura, Yoshihiro Sakoda, Tetsuya Osaka
SCIENTIFIC REPORTS
(2019)
Article
Electrochemistry
Ryota Saito, Yusuke Sato, Daisuke Takimoto, Sho Hideshima, Wataru Sugimoto
Article
Electrochemistry
Yusuke Sato, Sho Makino, Dai Mochizuki, Sho Hideshima, Wataru Sugimoto
Review
Chemistry, Multidisciplinary
Olga Guselnikova, Hyunsoo Lim, Hyun-Jong Kim, Sung Hyun Kim, Alina Gorbunova, Miharu Eguchi, Pavel Postnikov, Takuya Nakanishi, Toru Asahi, Jongbeom Na, Yusuke Yamauchi
Summary: This article reviews recent fabrication methods for surface-enhanced Raman spectroscopy (SERS) substrates, focusing on advanced nanoarchitecture based on noble metals, nanolayered 2D materials, and nanoarchitecturing with organic molecules. The use of SERS has been extensively investigated for its enhanced signal intensity and the ability to detect a very small number of molecules. Nanoarchitecture strategies are crucial in the development of SERS substrates. The article discusses recent achievements in the morphology of metallic nanostructures and outlines future directions for reproducible preparation and well-controlled nanoarchitecture. The use of nanolayered 2D materials as an alternative to noble metals and the hybridization with organic molecules to improve stability and selectivity of SERS sensing are also described.
Article
Nanoscience & Nanotechnology
Daisuke Takimoto, Keisuke Suzuki, Ryusuke Futamura, Taku Iiyama, Sho Hideshima, Wataru Sugimoto
Summary: In this study, the micropore-confinement effect is utilized to reduce the activation barrier of quinone-based redox-active molecules adsorption, resulting in a zero-overpotential redox reaction. This technique has the potential to guide the development of high-performance, rapid charging/discharging electrodes for energy-storage devices such as batteries, supercapacitors, and hybrid devices using organic materials.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Electrical & Electronic
Sho Hideshima, Hiroki Hayashi, Ryo Takeuchi, Shofarul Wustoni, Shigeki Kuroiwa, Takuya Nakanishi, Toshiyuki Momma, Tetsuya Osaka
Summary: This study investigates the effect of reduced graphene oxide (rGO) coating on the gate insulator of FET biosensors and demonstrates that the rGO coating can enhance the stability of the sensor in aqueous solution. Graphene prevents cations in the electrolyte from invading the gate insulator of the FET, and functionalizing the rGO-coated FET surface enables specific detection of target molecules.
MICROELECTRONIC ENGINEERING
(2022)
Article
Electrochemistry
Daisuke Takimoto, Keisuke Suzuki, Sho Hideshima, Wataru Sugimoto
Summary: Pseudocapacitive flowable electrodes with redoxactive organic materials confined in micropores of activated carbon exhibit bellshaped cyclic voltammograms and show a charge-transfer-controlled mechanism, which inhibits the fast charging and discharging of the electrode.
Article
Electrochemistry
Daisuke Takimoto, Keisuke Suzuki, Sho Hideshima, Wataru Sugimoto
Summary: Redox-active organic materials have advantages such as low cost and flexible design, making them promising alternatives to inorganic electrode materials in electrochemical devices. However, their electrochemical reactions are typically slow due to slow diffusion of organic materials in electrolyte. This study reports an approach to achieve an adsorption-controlled redox reaction of quinone-based molecules and analyzes their reaction environment.
Article
Materials Science, Multidisciplinary
Moeka Inada, Tatsuhiro Horii, Toshinori Fujie, Takuya Nakanishi, Toru Asahi, Kei Saito
Summary: Debonding-on-demand (DoD) adhesives, with the ability to repeatedly adhere and release in response to external stimuli, are attracting attention as sustainable functional materials. This study presents a DoD adhesive system based on a polymer fabricated by the reversible cycloaddition reaction of coumarin-terminated four-arm siloxane monomers. The thermophysical property analysis confirms that photoinduced fluidization is achieved through reversible crosslinking and decrosslinking reactions of the monomers. This simple siloxane-based reversible cycloaddition system exhibits significant potential as a room temperature DoD adhesive.
MATERIALS ADVANCES
(2023)
Article
Chemistry, Analytical
Sho Hideshima, Hiroki Hayashi, Sayoko Saito, Hiroaki Tateno, Toshiyuki Momma, Tetsuya Osaka
Summary: A non-destructive method using FET biosensors was developed to monitor the differentiation status of stem cells by detecting the glycoprotein biomarker podocalyxin. The FETs successfully detected the intrinsic charges of podocalyxin without labeling and monitored the decrease in its concentration during the differentiation process. This technique shows promise for simple and rapid monitoring of hiPSCs differentiation status.
ANALYSIS & SENSING
(2023)
Article
Chemistry, Analytical
Sho Hideshima, Shofarul Wustoni, Masumi Kobayashi, Hiroki Hayashi, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka
SENSING AND BIO-SENSING RESEARCH
(2018)