Article
Biotechnology & Applied Microbiology
Senthilkumar Muthusamy, Sathya Kannan, Marcus Lee, Vijayavenkataraman Sanjairaj, Wen Feng Lu, Jerry Y. H. Fuh, Gopu Sriram, Tong Cao
Summary: Bioprinting technology has made significant progress over the past decade, particularly in developing larger and more physiologic tissues. By precisely combining biological scaffold materials and cells in three-dimensional space, bioprinting has the potential to assist in locating endothelial cells in specific spatial positions to promote vessel formation in predefined areas.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Applied
Penghui Wang, Yajie Pu, Yanhan Ren, Rong Yang, Wenjie Zhang, Xiaoyan Tan, Wenliang Xue, Shuai Liu, Shuang Li, Bo Chi
Summary: This research introduces a novel hybrid hydrogel system based on sodium alginate and polyglutamic acid, with adjustable gelation time and mechanical strength, prolonged degradation time and reduced swelling rate, dynamic response characteristics, excellent self-healing and injectable ability. The system shows positive cytocompatibility and accelerated regulatory gene expression in cartilage tissue, indicating broad application prospects in future biomedical engineering.
CARBOHYDRATE POLYMERS
(2022)
Article
Biotechnology & Applied Microbiology
Simon F. Carroll, Conor T. Buckley, Daniel J. Kelly
Summary: This study investigated how factors such as cell source, environmental oxygen tension, and cell seeding density influence the local oxygen environment within engineered cartilaginous tissues, as well as their impact on cellular oxygen consumption rate and cartilage matrix synthesis. The findings suggest that promoting an oxygen consumption profile similar to that of chondrocytes may be a key factor in the success of stem cell-based cartilage tissue engineering strategies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Guanzhou Zhou, Ailing Tian, Xin Yi, Lufeng Fan, Wenchong Shao, Han Wu, Nianfeng Sun
Summary: The study demonstrated that Ad-MSCs from primary cultures exhibit stem cell characteristics, and self-assembled nanopeptide hydrogel is a good tissue engineering material. In the 3D-printed tissue model, Ad-MSCs grew well and retained strong differentiation ability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Tingting Yu, Lingyun Zhang, Xueyu Dou, Rushui Bai, Hufei Wang, Jie Deng, Yunfan Zhang, Qiannan Sun, Qian Li, Xing Wang, Bing Han
Summary: Development of artificial biomaterials that mimic the extracellular matrix of bone tissue is a promising strategy for bone regeneration. However, the current hydrogel substitutes have inhomogeneous networks and weak mechanics, which greatly hinder their clinical applications. In this study, a dual crosslinked gelling system with tunable architectures and mechanics was developed to promote osteogenic capacity. The hybrid hydrogel showed outstanding osteogenic potential both in vitro and in vivo, and a key epigenetic regulator of ten-eleven translocation 2 (Tet2) was discovered to play a significant role in promoting osteogenesis.
Review
Engineering, Biomedical
Xiaojie Liu, Tao Fang, Ting Shi, Yun Wang, Guanying Liu
Summary: The anatomical and physiological architecture of the craniofacial bone is complex, making precise management of osteogenesis crucial for regeneration of any deficiencies in this area. Stem-based tissue engineering approaches offer a safer and more cost-effective way to induce bone growth compared to traditional surgical intervention. Mesenchymal stem/stromal cells (MSCs) have pluripotent differentiation potential, anti-inflammatory and immunomodulatory properties that make them versatile in bone tissue therapies. Hydrogels, with their excellent swelling capabilities and similarity to natural extracellular matrices, are preferred choices for mediating cell growth and adapting to 3D environments. Due to their biocompatibility and ability to stimulate bone regeneration, hydrogels have become a topic of great interest in bone tissue engineering. This review explores the potential of MSC-based regenerative skeletal therapies and the application of hydrogel scaffolds as artificial bone microenvironments for stem cells in craniofacial bone tissue engineering.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2023)
Article
Engineering, Biomedical
P. Saez, C. Borau, N. Antonovaite, K. Franze
Summary: This study developed a model to predict local tissue mechanics, and found that considering individual tissue constituents alone cannot reproduce the mechanical properties of CNS tissue. The results suggest that the response of brain tissue to applied forces depends on the amount, stiffness, and assembly of the tissue constituents.
Article
Nanoscience & Nanotechnology
Vania I. B. Castro, Ana R. Araujo, Filipa Duarte, Antonio Sousa-Franco, Rui L. Reis, Iva Pashkuleva, Ricardo A. Pires
Summary: We developed biofunctional supramolecular hydrogels from an aromatic glycodipeptide using a bottom-up approach. The self-assembly of the glycopeptide was induced by temperature or solvent switch. The gels, triggered by salt in cell culture media, had different mechanical properties and promoted neural differentiation of human adipose derived stem cells. Glycosylation was found to be crucial for the biofunctionality of the hydrogels by capturing and preserving essential growth factors.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Applied
Xin Liu, Shuai Liu, Rong Yang, Penghui Wang, Wenjie Zhang, Xiaoyan Tan, Yanhan Ren, Bo Chi
Summary: A gradient hydrogel scaffold was designed based on the structure and composition distribution in biological cartilage tissue, showing excellent physical properties and cell compatibility. The scaffold can mimic the function of natural cartilage, making it a potential option for cartilage tissue engineering.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Multidisciplinary
Tina Arndt, Urmimala Chatterjee, Olga Shilkova, Juanita Francis, Johan Lundkvist, Daniel Johansson, Benjamin Schmuck, Gabriele Greco, Asa Ekblad Nordberg, Yan Li, Lars U. Wahlberg, Maud Langton, Jan Johansson, Cecilia Goetherstroem, Anna Rising
Summary: A recombinant spider silk protein-derived hydrogel that forms rapidly at 37 degrees C is developed as a drug release system and tissue engineering scaffold. The diffusion rate and mechanical properties of the hydrogel can be adjusted by changing the protein concentration. Encapsulated stem cells show high survival and viability, and bioactive molecules can be easily included in the gel and maintain their activity.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Mohammad Samiei, Elaheh Dalir Abdolahinia, Marziyeh Fathi, Jaleh Barar, Yadollah Omidi
Summary: In this study, chitosan-based hydrogels were used to improve the proliferation and differentiation of dental pulp stem cells (DPSCs), with CS/GN hydrogel showing better features in enhancing DPSCs viability and osteogenic differentiation.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Review
Polymer Science
Jian Yang, Haojie Yu, Li Wang, Jian Liu, Xiaowei Liu, Yichuan Hong, Yudi Huang, Shuning Ren
Summary: Adhesive hydrogels as emerging biomedical materials have attracted much attention for their potential applications in tissue engineering. They possess excellent adhesion, biocompatibility, degradability, and antibacterial properties, making them suitable for hemostatic agents, sealants, and other applications.
EUROPEAN POLYMER JOURNAL
(2022)
Review
Chemistry, Multidisciplinary
Xiang Lin, Jinglin Wang, Xiangyi Wu, Yuan Luo, Yongan Wang, Yuanjin Zhao
Summary: Marine organisms provide valuable resources for biomaterials, especially natural hydrogels, which have outstanding bioactivity and biocompatibility. These hydrogels can not only mimic tissue extracellular matrix but also be applied in biomedicine through various techniques. This review summarizes the classification and characteristics of marine-derived hydrogels, introduces their role in biomaterial development, and discusses the influence of hydrogel types on their functions in biomedical applications.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Ioannis Eugenis, Di Wu, Caroline Hu, Gladys Chiang, Ngan F. Huang, Thomas A. Rando
Summary: Volumetric muscle loss (VML) is an irreversible loss of skeletal muscle due to trauma or surgery. In this study, a macroporous dECM-methacrylate (dECM-MA) hydrogel was developed and found to promote cellularization, endothelialization, and establishment of a pro-regenerative immune microenvironment. The hydrogel also enhanced the proliferation of transplanted primary muscle stem cells, muscle tissue regeneration, and functional recovery.
Review
Polymer Science
Bernhard V. K. J. Schmidt
Summary: Hydrogels, with their soft and tissue-like properties and high water-content, are highly promising materials with a wide range of applications including tissue engineering and drug delivery. The properties of hydrogels can be tailored through crosslinking mechanisms, shape, and composition, with multicompartment hydrogels driving progress in complex and highly functional soft materials. This review highlights the latest developments in multicompartment hydrogels, focusing on various compartments and discussing their morphologies and applications. The further development of multicompartment hydrogels will have a significant impact on biomedicine and organic devices.
MACROMOLECULAR RAPID COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Wenguang Wang, Haiheng Xu, Qingsong Ye, Feng Tao, Ian Wheeldon, Ahu Yuan, Yiqiao Hu, Jinhui Wu
Summary: Flagellated bacteria coated with antigen-adsorbing polymer nanoparticles and injected into tumours in mice after radiotherapy elicit systemic antitumour effects by transporting antigens to the tumour's periphery. This finding highlights the potential of leveraging bacteria to enhance dendritic cell activation and improve in situ cancer vaccination strategies.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Brian Lupish, Jordan Hall, Cory Schwartz, Adithya Ramesh, Clifford Morrison, Ian Wheeldon
Summary: Yarrowia lipolytica is a yeast strain of interest for metabolic engineering due to its ability to metabolize various renewable carbon sources. Through a genome-wide CRISPR screen, we identified the Delta RAS2 mutant as the only one that inhibits hyphal formation without affecting lycopene production. This suggests that the Delta RAS2 mutant could be useful for engineering the production of carotenoids and other biochemicals.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Nanoscience & Nanotechnology
Arpita Iddya, Piotr Zarzycki, Ryan Kingsbury, Chia Miang Khor, Shengcun Ma, Jingbo Wang, Ian Wheeldon, Zhiyong Jason Ren, Eric M. Hoek, David Jassby
Summary: This study demonstrates a nanocomposite ion-exchange membrane material that enables a reverse-selective transport mechanism with high selectivity. By incorporating hydrous manganese oxide nanoparticles into the membrane, the phosphate flux is significantly increased and the selectivity for phosphate ions over other ions is greatly enhanced.
NATURE NANOTECHNOLOGY
(2022)
Article
Materials Science, Biomaterials
Marvin Mecwan, Reihaneh Haghniaz, Alireza Hassani Najafabadi, Kalpana Mandal, Vadim Jucaud, Johnson V. John, Ali Khademhosseini
Summary: Researchers have successfully engineered a thermoresponsive shear-thinning hydrogel, composed of a thermoresponsive polymer and hemostatic silicate nanodisks, as an injectable hemostat. This hydrogel demonstrates rapid mechanical recovery and temperature-dependent blood coagulation, effectively preventing blood loss. It shows comparable efficacy to a commercially available hemostat and can be easily removed using a cold saline wash.
BIOMATERIALS SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Gang Ge, Kalpana Mandal, Reihaneh Haghniaz, Mengchen Li, Xiao Xiao, Larry Carlson, Vadim Jucaud, Mehmet Remzi Dokmeci, Ghim Wei Ho, Ali Khademhosseini
Summary: Adhesive materials have been receiving increasing attention for their excellent sealing ability. However, existing adhesives typically have weak adhesion strength and deteriorate in harsh environments. In this study, a novel ionogel with tunable mechanical properties is developed using a deep eutectic solvent as the medium for photopolymerization. The ionogel demonstrates fast gelation and bonding without external pressure, high adhesion in various environments, and wide applications in packaging, marine engineering, medical adhesives, and electronic assembly.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cell & Tissue Engineering
Pauline Cheng, Ahmad Rashad, Ankit Gangrade, Natan Roberto de Barros, Ali Khademhosseini, Jonathan Tam, Padmini Varadarajan, Devendra K. Agrawal, Finosh G. Thankam
Summary: Myocardial infarction leads to the loss of cardiomyocytes, but stem cell therapy shows promise in restoring cardiac function. This article discusses the application of different stem cell phenotypes and strategies to promote their differentiation into cardiomyocytes.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Engineering, Biomedical
Hossein Montazerian, Alireza Hassani Najafabadi, Elham Davoodi, Rasoul Seyedmahmoud, Reihaneh Haghniaz, Avijit Baidya, Wei Gao, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Mussel-inspired catechol-functionalization of degradable natural biomaterials is proposed as a potential approach to achieve bioadhesion for sutureless wound closure. This approach involves a simple oxidative polymerization step before conjugation of catechol-carrying molecules to amplify catechol function in bioadhesion. The modified gelatin with poly(l-DOPA) moieties shows improved wound control and enhanced cohesion, surpassing commercial sealants, and also possesses photothermal responsiveness and antibacterial activity, making it an effective biomaterial design strategy for wound closure applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biology
Adithya Ramesh, Varun Trivedi, Sangcheon Lee, Aida Tafrishi, Cory Schwartz, Amirsadra Mohseni, Mengwan Li, Stefano Lonardi, Ian Wheeldon
Summary: acCRISPR is a method that corrects the inaccuracies in CRISPR screening results caused by variability in sgRNA cutting efficiency by computing a fitness score for each targeted gene. It is used to identify essential genes and their fitness effects in CRISPR screens. The study applied acCRISPR to identify essential genes for growth under glucose and salt tolerance conditions in the yeast Yarrowia lipolytica. This work presents an experimental-computational framework for CRISPR-based functional genomics studies in non-conventional organisms.
COMMUNICATIONS BIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Hossein Montazerian, Shameek Mitra, Alireza Hassani Najafabadi, Rasoul Seyedmahmoud, Yuting Zheng, Mehmet Remzi Dokmeci, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Inspired by mussel adhesion, catechol functionalization has been widely used to impart adhesion to biomaterials. However, the bioadhesion capacity of catechol motifs in hydrogels that crosslink through free-radical-based systems is debated. This study reevaluates the bioadhesion efficacy of catechol functionalized gelatin biomolecules in methacryloyl-modified photo-cross-linkable biomaterials, and investigates the effects of catechol content on adhesion strength and hydrogel integrity. The cytotoxic and immunostimulatory effects of catechol groups are also evaluated for clinical applications.
ACS MATERIALS LETTERS
(2023)
Article
Biotechnology & Applied Microbiology
Sarah Thorwall, Varun Trivedi, Eva Ottum, Ian Wheeldon
Summary: The emergence of next-generation sequencing (NGS) technologies has enabled the identification of metabolic engineering targets in microbial populations. This study used NGS data and bioinformatics tools to identify genomic signatures for improving phenazine biosynthesis in Pseudomonas chlororaphis. By sequencing and analyzing different Pseudomonas isolates, the study identified several genes that significantly influenced phenazine production. Overall, this work demonstrates the value of population genomics in identifying targets for metabolic engineering in bioproduction hosts.
METABOLIC ENGINEERING
(2023)
Article
Chemistry, Analytical
Safoora Khosravi, Saeid Soltanian, Amir Servati, Ali Khademhosseini, Yangzhi Zhu, Peyman Servati
Summary: Wearable sweat biosensors embedded in textile substrates have gained attention for noninvasive monitoring of health parameters. A flexible electrochemical glucose sensor that can be screen-printed onto a textile substrate is demonstrated, achieving a linear response in the range of 20-1000μM of glucose concentration with high sensitivity and stability. These textile-based biosensors show high selectivity towards glucose and have the potential to impact the next generation of wearable devices.
Review
Biotechnology & Applied Microbiology
Varun Trivedi, Adithya Ramesh, Ian Wheeldon
Summary: The multifaceted nature of CRISPR screens has driven advancements in functional genomics. However, the application of CRISPR screens in non-conventional yeasts and the lack of accurate bioinformatic algorithms for analyzing microbial screening data have been limitations. This mini-review explores the potential and methods for using CRISPR screens in non-conventional microbes and highlights the importance of developing accurate algorithms for data analysis.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Review
Automation & Control Systems
Baishali Kanjilal, Yangzhi Zhu, Vaishali Krishnadoss, Janitha M. M. Unagolla, Parnian Saemian, Alessia Caci, Danial Cheraghali, Iman Dehzangi, Ali Khademhosseini, Iman Noshadi
Summary: Ionic liquids (ILs) have unique properties that make them promising candidates for biomedical applications, but their cytocompatibility limitations are enhanced by using bioionic liquids (BILs) derived from biological molecules. BILs can be synthesized and immobilized onto biopolymers, and their functionalization enables the design of responsive actuators and sensors. The cytocompatibility of BIL-functionalized polymers also makes them suitable for power storage and implantable devices. This review focuses on the recent advances of BILs in biomedical applications, specifically their use as functionalization agents for biopolymers and solvents for supermolecular ionic networks.
ADVANCED INTELLIGENT SYSTEMS
(2023)
Article
Chemistry, Multidisciplinary
Bruna Gregatti Carvalho, Aya Nakayama, Hiromi Miwa, Sang Won Han, Lucimara Gaziola de la Torre, Dino Di Carlo, Junmin Lee, Han-Jun Kim, Ali Khademhosseini, Natan Roberto de Barros
Summary: A novel mRNA-releasing matrix based on GelMA microporous annealed particle scaffolds is reported. The sustained release of mRNA complexes achieves indirect intracellular delivery, while direct intracellular delivery is achieved by cell adhesion on the mRNA-releasing scaffolds. This hybrid system demonstrates efficient protein expression, offering potential for mRNA-releasing biomaterials in tissue engineering.
Review
Chemistry, Multidisciplinary
Hossein Montazerian, Elham Davoodi, Avijit Baidya, Maryam Badv, Reihaneh Haghniaz, Arash Dalili, Abbas S. Milani, Mina Hoorfar, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: This review provides a biomacromolecular design roadmap for the development of tough adhesive surgical sealants. The intrinsic toughness and elasticity of polymers are achieved through the introduction of strong and dynamic inter- and intramolecular interactions, either through polymer chain design or the use of crosslink regulating additives. Efforts have also been made to promote underwater adhesion through covalent/noncovalent bonds and micro/macro-interlock mechanisms. The measurement and reporting requirements for fair comparisons of different materials and their properties are discussed.
CHEMICAL SOCIETY REVIEWS
(2022)
