Article
Multidisciplinary Sciences
Sophie Hallakou-Bozec, Micheline Kergoat, Pascale Fouqueray, Sebastien Bolze, David E. Moller
Summary: Imeglimin, a novel drug candidate, enhances glucose-stimulated insulin secretion in islets isolated from rodents with Type 2 diabetes through increasing the NAD(+) pool, raising ATP levels, and promoting cADPR production. This unique mode of action suggests a potential link between elevated NAD(+) and improved glucose-induced Ca2+ mobilization, leading to enhanced insulin secretion and providing a new approach for treating patients with Type 2 diabetes.
Article
Endocrinology & Metabolism
Aaron L. Glieberman, Benjamin D. Pope, Douglas A. Melton, Kevin Kit Parker
Summary: Diabetes is a disease of insulin insufficiency, where islet transplantation offers a potential therapy, but is still limited by the availability of human islets and reliable function testing. Stem cell technologies may fill the shortage of transplantable cells, yet better methods are needed to predict transplantation outcomes.
Article
Endocrinology & Metabolism
Alicia White, Jane Stremming, Brit H. Boehmer, Eileen I. Chang, Sonnet S. Jonker, Stephanie R. Wesolowski, Laura D. Brown, Paul. J. Rozance
Summary: After a 1-week infusion of IGF-1 LR3, late gestation fetal sheep showed lower plasma insulin and glucose concentrations, reduced fetal glucose-stimulated insulin secretion, and decreased fractional insulin secretion from isolated fetal islets without changes in pancreatic insulin content.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biotechnology & Applied Microbiology
Patricia Wu Jin, Nassim Rousset, Andreas Hierlemann, Patrick M. Misun
Summary: A new generation microfluidic hanging-drop-based islet perifusion platform was developed to study dynamic insulin secretion from individual pancreatic islets with high temporal resolution. The platform was redesigned to increase experimental throughput and simplify operation, achieving continuous insulin release observation with reproducible results. This device will be valuable in islet and diabetes research for studying alterations in insulin secretion dynamics.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Estifanos N. Habtemichael, Don T. Li, Joao Paulo Camporez, Xavier O. Westergaard, Chloe Sales, Xinran Liu, Francesc Lopez-Giraldez, Stephen G. DeVries, Hanbing Li, Diana M. Ruiz, Kenny Y. Wang, Bhavesh S. Sayal, Sofia Gonzalez Zapata, Pamela Dann, Stacey N. Brown, Sandro Hirabara, Daniel F. Vatner, Leigh Goedeke, William Philbrick, Gerald Shulman, Jonathan S. Bogan
Summary: Insulin-stimulated TUG cleavage pathway regulates gene expression to promote lipid oxidation and thermogenesis, linking glucose uptake to organismal energy expenditure. Attenuation of this mechanism may promote obesity as it contributes to the thermic effect of food.
Article
Multidisciplinary Sciences
Morgana Barroso Oquendo, Estela Lorza-Gil, David Juarez-Lopez, Robert Wagner, Andreas L. Birkenfeld, Susanne Ullrich, Felicia Gerst
Summary: Inflammatory cytokines and non-esterified fatty acids (NEFAs) that are associated with obesity can disrupt insulin secretion. This study found that an overload of NEFA/cytokines in pancreatic adipose tissue may impact insulin secretion.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
Bin Yang, Vasily M. Gelfanov, Diego Perez-Tilve, Barent DuBois, Rebecca Rohlfs, Jay Levy, Jonathan D. Douros, Brian Finan, John P. Mayer, Richard D. DiMarchi
Summary: Chemical optimization of truncated glucagon fragments led to the identification of a potent antagonist peptide that successfully reversed glucagon-induced glucose elevation in animals. Substitution of specific amino acids further improved receptor binding and inhibitory potency, demonstrating enhanced antagonist effects. A palmitoylated analogue displayed sustained in vivo action, highlighting the potential for long-lasting glucose-lowering effects.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Maysam Mansouri, Preetam Guha Ray, Nik Franko, Shuai Xue, Martin Fussenegger
Summary: The development of novel strategies to program cellular behaviors is a central goal in synthetic biology. In this study, we have developed a programmable protease-mediated post-translational switch (POSH) control platform that allows rapid protein secretion on demand. Different stimuli, including plant-derived chemicals, clinically compatible chemicals, and remotely controllable inducers, can program protein secretion in engineered mammalian cells. This platform has potential applications in various fields, such as drug delivery and tissue engineering.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Endocrinology & Metabolism
Han-Chow E. Koh, Stephan van Vliet, Gretchen A. Meyer, Richard Laforest, Robert J. Gropler, Samuel Klein, Bettina Mittendorfer
Summary: The study aimed to explore the relationships among muscle fibre type composition, perfusion, and insulin-stimulated glucose uptake rates in healthy lean individuals and those with obesity. Results showed that obesity-associated insulin resistance is generalized across all major muscles and is not influenced by alterations in muscle fibre type composition or perfusion. Additionally, insulin-stimulated whole-body glucose disposal relative to fat-free mass serves as a reliable indicator of muscle glucose uptake rate.
Article
Biochemistry & Molecular Biology
Emily Esakov, Neha Nandedkar-Kulkarni, Ali G. Al-Dieri, Hannah Hafner, Brigid Gregg, Marcia F. McInerney
Summary: The newly established CD3FLAG-mIR transgenic mouse model shows phenotypic and metabolic changes in islets, characterized by enlargement, disorganization, increased serum insulin, and glucose intolerance. Transplantation of transgenic spleen cells into another mouse model also resulted in similar changes in islets, indicating the important impact of T cells on islet organization and function.
Article
Endocrinology & Metabolism
Shee Chee Ong, Alessia Belgi, Allanah L. Merriman, Carlie A. Delaine, Bianca van Lierop, Sofianos Andrikopoulos, Andrea J. Robinson, Briony E. Forbes
Summary: The mechanisms of how insulin activates its receptor and promotes metabolic processes and cellular growth are still unclear. This study investigates novel insulin analogues with different chemical compositions to understand signaling bias and finds that analogues with an A6-A11 cis-dicarba bond have low mitogenic potency. These findings will assist in developing insulin analogues for diabetes treatment with low mitogenic activity and reduced risk of promoting cancer.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Paola A. Ramos, Kelli A. Lytle, Danae Delivanis, Soren Nielsen, Nathan K. LeBrasseur, Michael D. Jensen
Summary: The study found that there were no defects in insulin receptor signaling at the Akt/AS160 level in the muscles of obese individuals, indicating uncertainties in the mechanism of muscle insulin resistance in obesity.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Review
Endocrinology & Metabolism
John T. Walker, Diane C. Saunders, Marcela Brissova, Alvin C. Powers
Summary: This review discusses the structure, function, and pathophysiology of human pancreatic islets, as well as the changes in islets in response to physiological challenges and diabetes. It also outlines current and future interventions for protecting, restoring, or replacing human islets. Additionally, the review highlights unresolved questions about human islets and areas needing further research.
Article
Biochemical Research Methods
Emma L. Vanderlaan, Joshua Sexton, Carmella Evans-Molina, Adrian Buganza Tepole, Sherry L. Voytik-Harbin
Summary: This study presents a novel three-dimensional microphysiological system (MPS) that improves the health and function of pancreatic beta cells. By incorporating a polymerizable collagen scaffold, the MPS restores biophysical support and mechanobiological cues to the islets, leading to increased viability and function. Experimental results demonstrate the effectiveness of the MPS compared to traditional suspension culture formats.
Article
Environmental Sciences
Xiaoyu Duan, Weijie Sun, Hongwen Sun, Lianying Zhang
Summary: The study revealed that PFASs adversely affect insulin secretion, with exposure to PFOS inhibiting SIRT1 activity and leading to impairment of GSIS.
ENVIRONMENTAL POLLUTION
(2021)
Editorial Material
Medicine, General & Internal
T. Sawano, M. Takita, Y. Senoo, Y. Nishikawa, A. Crump, M. Tsubokura
QJM-AN INTERNATIONAL JOURNAL OF MEDICINE
(2023)
Article
Multidisciplinary Sciences
Kana Yamamoto, Morihito Takita, Masahiro Kami, Masaharu Tsubokura, Tetsuya Tanimoto, Toshio Kitamura, Yoshinobu Takemoto
Summary: Anemia is prevalent among adolescent students in sports clubs, with both iron deficiency and excessive training contributing to the risk. Physicians should take exercise into account when evaluating anemia in clinical practice.
Article
Immunology
Makoto Yoshida, Yurie Kobashi, Takeshi Kawamura, Yuzo Shimazu, Yoshitaka Nishikawa, Fumiya Omata, Tianchen Zhao, Chika Yamamoto, Yudai Kaneko, Aya Nakayama, Morihito Takita, Naomi Ito, Moe Kawashima, Sota Sugiura, Kenji Shibuya, Shingo Iwami, Kwangsu Kim, Shoya Iwanami, Tatsuhiko Kodama, Masaharu Tsubokura
Summary: This retrospective cohort study aimed to investigate factors associated with hesitancy towards receiving a third dose of COVID-19 vaccine. Younger age and higher antibody levels were found to be positively associated with hesitancy. Rapid increase in booster dose rate is necessary to control the pandemic, and specific approaches should be taken to address hesitancy in certain groups.
Article
Medicine, General & Internal
Ryusei Otsuka, Chika Miyagi-Shiohira, Kazuho Kuwae, Kai Nishime, Yoshihito Tamaki, Tasuku Yonaha, Mayuko Sakai-Yonaha, Ikuo Yamasaki, Misaki Shinzato, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi
Summary: Pancreatic preservation with alvelestat improves islet yield and graft function, and may serve as a novel clinical strategy for improving the outcome of islet transplantation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Editorial Material
Medicine, General & Internal
Hirofumi Noguchi
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Natsumi Ibano, Emi Inada, Shinji Otake, Yuki Kiyokawa, Kensuke Sakata, Masahiro Sato, Naoko Kubota, Hirofumi Noguchi, Yoko Iwase, Tomoya Murakami, Tadashi Sawami, Yoshito Kakihara, Takeyasu Maeda, Miho Terunuma, Yutaka Terao, Issei Saitoh
Summary: This study aimed to elucidate the role of genetically modified human feeder cells expressing growth factors in maintaining the integrity of primary cultured human deciduous dental pulp cells. The results showed that co-culturing enhanced cell proliferation, promoted differentiation, and maintained stemness properties.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Immunology
Moe Kawashima, Hiroaki Saito, Takamitsu Nishiuchi, Hiroki Yoshimura, Masatoshi Wakui, Yuta Tani, Yoshitaka Nishikawa, Fumiya Omata, Morihito Takita, Tianchen Zhao, Chika Yamamoto, Yurie Kobashi, Takeshi Kawamura, Akira Sugiyama, Aya Nakayama, Yudai Kaneko, Toyoaki Sawano, Kenji Shibuya, Junichiro Kazama, Ryuzaburo Shineha, Masaharu Tsubokura
Summary: Intensive vaccination is recommended for vulnerable populations to COVID-19, but data on dialysis patients' immunity after vaccination are lacking. This study examined antibody levels, neutralizing activity, and interferon gamma levels in dialysis patients after receiving the BNT162b2 or mRNA-1273 vaccine. The study found that most dialysis patients developed both humoral and cellular immunity against SARS-CoV-2.
Letter
Public, Environmental & Occupational Health
T. Uchiyama, M. Takita, H. Yonemura, M. Tsubokura, K. Shibuya
Article
Immunology
Yuta Tani, Morihito Takita, Yurie Kobashi, Masatoshi Wakui, Tianchen Zhao, Chika Yamamoto, Hiroaki Saito, Moe Kawashima, Sota Sugiura, Yoshitaka Nishikawa, Fumiya Omata, Yuzo Shimazu, Takeshi Kawamura, Akira Sugiyama, Aya Nakayama, Yudai Kaneko, Tetsuhiko Kodama, Masahiro Kami, Masaharu Tsubokura
Summary: A study in Fukushima, Japan, evaluated the proportion of individuals with cellular immunity after booster vaccination using the T-SPOT.COVID test. It was found that 64.3% of participants had reactive cellular immunity, with age<40 and adverse reactions after vaccination being independent predictors of cellular immunity. Notably, a significant proportion of participants with high antibody titers did not have reactive cellular immunity.
Letter
Geriatrics & Gerontology
Yuna Uchi, Erika Yamashita, Masahiro Kami, Morihito Takita
ARCHIVES OF GERONTOLOGY AND GERIATRICS
(2023)
Article
Medicine, General & Internal
Fumiue Harada, Morihito Takita, Kana Yamamoto, Yosuke Tachiya, Chika Yamamoto, Hiroaki Saito, Masahiro Kami, Ryuzaburo Shineha
Summary: Audio-only encounters can help diagnose herpes zoster if follow-up examinations are performed adequately. Audio-only encounters preserve the flexibility of telemedicine when video communication cannot be prepared promptly.
CLINICAL MEDICINE INSIGHTS-CASE REPORTS
(2023)
Article
Biochemical Research Methods
Hirofumi Noguchi, Yoshiki Nakashima, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara, Issei Saitoh, Chika Miyagi-Shiohira
Summary: This study presents a protocol to reprogram human hepatocytes into induced tissue-specific liver stem cells, which continuously express hepatocyte-specific markers and do not form teratomas.
Letter
Medicine, General & Internal
Yuki Murata, Morihito Takita, Masahiro Kami
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Letter
Medicine, General & Internal
Yuki Murata, Morihito Takita, Masahiro Kami
Summary: This study demonstrates that a closed-loop insulin-delivery system is superior to glucose sensor-augmented insulin-pump therapy for glycemic control in children aged 1 to 7 with type 1 diabetes. However, it should be noted that unscheduled contacts with trial staff are more frequent during the closed-loop period.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Medicine, General & Internal
Erika Yamashita, Morihito Takita, Masahiro Kami
Summary: This study investigates the variation in death reports after COVID-19 vaccination among the older population in Japan, the US, and European countries. The results show an early increase in death reports on Day 2 after vaccination in all three regions. There is also a certain degree of time-dependence in the female-to-male ratio in Japan, but not in the US and European countries.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2022)