Article
Microbiology
Fengjiao Du, Aiying Xing, Zihui Li, Liping Pan, Hongyan Jia, Boping Du, Qi Sun, Rongrong Wei, Zhongquan Liu, Zongde Zhang
Summary: The Rpfs-TLA culture method is an accurate, rapid, cheap, and easy technique for the quick detection of Mycobacterium tuberculosis in pleural fluid.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Sarah M. Glenn, Obolbek Turapov, Vadim Makarov, Douglas B. Kell, Galina V. Mukamolova
Summary: Tuberculosis (TB) is a deadly disease, claiming the lives of nearly 1.5 million people each year. Current treatment involves a combination of drugs administered for at least 6 months. However, a study has shown that adding an immunomodulatory drug to the standard treatment can eliminate difficult-to-eradicate bacteria, known as differentially culturable bacteria (DCB), within 8 weeks, potentially improving TB treatment and reducing its duration.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Geethu S. Kumar, Amit Dubey, Siva Prasad Panda, Maha M. Alawi, Anees A. Sindi, Esam I. Azhar, Vivek Dhar Dwivedi, Sharad Agrawal
Summary: Tuberculosis infection is a global public health concern with increasing mortality rates. Resuscitation of Mycobacterium tuberculosis (Mtb) from dormant state is a major factor contributing to the spread of tuberculosis, especially latent tuberculosis infection (LTBI). RpfB, responsible for resuscitation, is a potential target for developing tuberculosis drugs. In this study, a computational screening of antibiotics against the ligand binding region of RpfB protein was carried out, and three antibiotics (Amikacin hydrate, Isepamicin sulphate, and Bekanamycin) were selected as potential inhibitors based on their binding free energy values. Molecular dynamic simulations and analysis confirmed the stability of these antibiotics with RpfB protein. In vivo and in vitro studies are needed to validate their therapeutic activity against tuberculosis infection.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Microbiology
Catherine Vilcheze, Steven A. Porcelli, John Chan, William R. Jacobs
Summary: Study found that specific strains of Mycobacterium tuberculosis can alter their bactericidal properties through leucine and pantothenate auxotrophy, rather than arginine auxotrophy. These triple-auxotrophic strains are crucial for understanding the bacterial and host factors leading to persistent infection of M. tuberculosis as well as for vaccine development studies.
Article
Microbiology
Robin Smyth, Stefania Berton, Nusrah Rajabalee, Therese Chan, Jim Sun
Summary: Tuberculosis is a deadly infectious lung disease caused by Mycobacterium tuberculosis. Strategies that boost host immunity to restrict intracellular replication of Mtb hold promise to overcome antimicrobial resistance in TB therapy. Protein Kinase R represents a novel and attractive host target for the development of alternative host-directed therapies for TB.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Manikuntala Kundu, Joyoti Basu
Summary: Mycobacterium tuberculosis can survive in its host for extended periods, remaining dormant within granulomas. Understanding the mechanisms of entry into and exit from dormancy is crucial for developing new tuberculosis treatment strategies. Transcriptomic and proteomic approaches have revealed key genes and pathways involved in dormancy and reactivation of M. tuberculosis.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Elena G. Salina, Vadim Makarov
Summary: Both latent and active TB infections are caused by a heterogeneous population of mycobacteria, and understanding the mechanisms underlying dormant and resuscitation phases of M. tuberculosis can help control latent infection and prevent transmission.
Article
Immunology
Ying Luo, Ying Xue, Liyan Mao, Qun Lin, Guoxing Tang, Huijuan Song, Wei Liu, Shutao Tong, Hongyan Hou, Min Huang, Renren Ouyang, Feng Wang, Ziyong Sun
Summary: The study demonstrated that HLA-DR on MTB-specific cells is a potentially useful biomarker for accurately discriminating between ATB and LTBI individuals.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Muhammad Junaid, Cheng-Dong Li, Jiayi Li, Abbas Khan, Syed Shujait Ali, Syed Baber Jamal, Shah Saud, Arif Ali, Dong-Qing Wei
Summary: The study focused on the resistance mechanisms offered by mutations Q10P, D12A, and G97D in Pyrazinamidase. It was found that Q10P and D12A mutations disrupt the communication among the catalytic triad, affecting the formation of the oxyanion hole and reducing the binding affinity to the drug. These mutations destabilize the interaction between Fe2+ ion and specific residues, ultimately impacting the binding pocket volume and PZA binding. The study provides insight for the design of new and effective PZase inhibitors.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Immunology
Saad H. Alotaibi
Summary: In this study, the compound DFMBT was synthesized and evaluated for its activity against Mycobacterium tuberculosis. Results showed that DFMBT exhibited bacteriostatic and bactericidal effects on M.tb H37RV. It had synergistic interactions with INH, RIF, and AMK, and an additive interaction with LVX. DFMBT also showed a decent post antibiotic effect, mutation frequency, and mutant prevention concentration. It may be developed as an effective agent for anti-TB therapy.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Carlos A. Guerrero-Bustamante, Rebekah M. Dedrick, Rebecca A. Garlena, Daniel A. Russell, Graham F. Hatfull
Summary: Research suggests using bacteriophages may be a promising strategy for treating tuberculosis, with a five-phage cocktail effectively killing Mycobacterium tuberculosis strains without affecting antibiotic effectiveness. This offers new possibilities for treating tuberculosis and combating antibiotic resistance.
Article
Microbiology
Chenling Xu, Yan Yue, Sidong Xiong
Summary: When infecting macrophages, the upregulation of Rv0928 protein by Mycobacterium tuberculosis (Mtb) enhances the immune defense response of macrophages, leading to increased apoptosis and inflammation, ultimately resulting in a dramatic reduction in intracellular bacterial load.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Mariateresa Coppola, Rachel P-J. Lai, Robert J. Wilkinson, Tom H. M. Ottenhoff
Summary: This study fills the knowledge gap regarding pulmonary expression of Mtb genes, revealing high concordance in the in vivo expressed genes among different datasets and species. It suggests that the consistently and highly expressed genes in vivo could be novel targets for future TB vaccine and drug development.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Akanksha Verma, Antara Ghoshal, Ved Prakash Dwivedi, Ashima Bhaskar
Summary: Mycobacterium tuberculosis is an intracellular pathogen that induces dormancy, counteracting host stress responses, and can resuscitate when conditions are favorable. Understanding key bacterial proteins can lead to novel therapies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Melissa D. Chengalroyen, Germar M. Beukes, Kennedy Otwombe, Bhavna G. Gordhan, Neil Martinson, Bavesh Kana
Summary: This study describes how DCTB assays can uncover differentially culturable tubercle bacilli in TB-infected individuals, leading to the discovery of mixed infections. Beijing mixed infections are associated with a higher bacterial load and reduced reliance on growth stimulatory factors. These findings have important implications for identifying mixed infections and hetero-resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Lei Cao, Fengfeng Mao, Zheng Pang, Yao Pi, Feng Qiu, Ruiguang Tian, Qingling Meng, Zhiyuan Jia, Shengli Bi
MOLECULAR MEDICINE REPORTS
(2015)
Article
Microbiology
Wenhui He, Bijie Ren, Fengfeng Mao, Zhiyi Jing, Yunfei Li, Yang Liu, Bo Peng, Huan Yan, Yonghe Qi, Yinyan Sun, Ju-Tao Guo, Jianhua Sui, Fengchao Wang, Wenhui Li
Article
Virology
Wenhui He, Zhiliang Cao, Fengfeng Mao, Bijie Ren, Yunfei Li, Dan Li, Huiyu Li, Bo Peng, Huan Yan, Yonghe Qi, Yinyan Sun, Fengchao Wang, Jianhua Sui, Wenhui Li
JOURNAL OF VIROLOGY
(2016)
Article
Biology
Dan Li, Wenhui He, Ximing Liu, Sanduo Zheng, Yonghe Qi, Huiyu Li, Fengfeng Mao, Juan Liu, Yinyan Sun, Lijing Pan, Kaixin Du, Keqiong Ye, Wenhui Li, Jianhua Sui
Article
Urology & Nephrology
Yuxin Li, Li Xue, Qing Miao, Fengfeng Mao, Liping Yao, Jianlin Yuan, Weijun Qin, Yufeng Zhao, Hang Sun, Fei Liu, He Wang
Article
Biochemistry & Molecular Biology
Lei Zhang, Hai Zhang, Yong Zhao, Fengfeng Mao, Jing Wu, Bing Bai, Zhikai Xu, Ying Jiang, Changhong Shi
DNA AND CELL BIOLOGY
(2012)
Article
Medical Laboratory Technology
Caiqin Zhang, Xiaoqin Song, Yong Zhao, Hai Zhang, Shanmin Zhao, Fengfeng Mao, Bing Bai, Shaoping Wu, Changhong Shi
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2015)
Article
Multidisciplinary Sciences
Shanmin Zhao, Yong Zhao, Fengfeng Mao, Caiqin Zhang, Bing Bai, Hai Zhang, Changhong Shi, Zhikai Xu
Article
Biochemistry & Molecular Biology
Fengfeng Mao, Teng Liu, Xinfeng Hou, Hanqing Zhao, Wenhui He, Cong Li, Zhiyi Jing, Jianhua Sui, Fengchao Wang, Xiaohui Liu, Jun Han, Christoph H. Borchers, Jian-She Wang, Wenhui Li
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Gastroenterology & Hepatology
Fengfeng Mao, Meng-Xuan Wang, Xinfeng Hou, Zhongmin Zhou, Yan-Yan Yan, Ling-Juan Fang, Zexi Tan, Wei-Yuan Fang, Teng Liu, Wenhui He, Cong Li, Xin-Bao Xie, Shi-Qi Lu, Jianhua Sui, Fengchao Wang, Jun Han, Jian-She Wang, Wenhui Li
Summary: NTCP is a bile acid transporter and serves as an entry receptor for hepatitis B virus and hepatitis D virus. Genetic variation in NTCP can cause hypercholanemia in neonates. Gallbladder abnormalities are found in mice and human infants with NTCP variants.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Chemistry, Medicinal
Yang Liu, Hanying Ruan, Ying Li, Guoliang Sun, Xiao Liu, Wenhui He, Fengfeng Mao, Miaomiao He, Liwei Yan, Guocai Zhong, Huan Yan, Wenhui Li, Zhiyuan Zhang
Summary: The analogue 27A, derived from natural cyclosporine A, shows potent inhibition of HBV/HDV infection by blocking viral engagement to NTCP, without immunosuppressive activity. It serves as a promising lead for novel anti-HDV/HBV agents.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Xueying Wang, Huabin Ruan, Zhaoyun Zong, Fengfeng Mao, Yusong Wang, Yupei Jiao, Lina Xu, Tao Yang, Wenhui Li, Xiaohui Liu
Summary: This study introduces a simulation assisted strategy for analyzing fatty acids, surpassing the limitations of using chemical standards, while also highlighting the importance of background interference and quantifiable serum volume in fatty acid analysis.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Virology
Rui Song, Xiaoyou Chen, Baoliang Li, Hongbin Zhang, Xiaodi Guo, Zhe Liu, Liangfeng Zou, Xiao Liang, Cong Lei, Fengfeng Mao, Jianhua Sui, Wenhui Li, Ronghua Jin
Summary: HH-120, an IgM-like ACE2 fusion protein, has shown promising antiviral efficacy in SARS-CoV-2-infected subjects as a nasal spray. In a single-arm trial, participants receiving the HH-120 nasal spray had significantly shorter viral clearance time compared to the control group. The HH-120 nasal spray demonstrated a favorable safety profile with mild, transient adverse events. Further large-scale randomized controlled clinical trials are needed to confirm the efficacy and safety of the HH-120 nasal spray.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Juan Liu, Fengfeng Mao, Jianhe Chen, Shuaiyao Lu, Yonghe Qi, Yinyan Sun, Linqiang Fang, Man Lung Yeung, Chunmei Liu, Guimei Yu, Guangyu Li, Ximing Liu, Yuansheng Yao, Panpan Huang, Dongxia Hao, Zibing Liu, Yu Ding, Haimo Liu, Fang Yang, Pan Chen, Rigai Sa, Yao Sheng, Xinxin Tian, Ran Peng, Xue Li, Junmian Luo, Yurui Cheng, Yule Zheng, Yongqing Lin, Rui Song, Ronghua Jin, Baoying Huang, Hyeryun Choe, Michael Farzan, Kwok-Yung Yuen, Wenjie Tan, Xiaozhong Peng, Jianhua Sui, Wenhui Li
Summary: Researchers have developed HH-120, an inhalable molecule with high avidity, which can neutralize all known variants of SARS-CoV-2 and significantly reduce viral loads and lung pathology scores. This study represents a meaningful advancement in inhalation delivery of large biologics and demonstrates the efficacy of HH-120 against SARS-CoV-2 variants.
NATURE COMMUNICATIONS
(2023)