期刊
INTERFACE FOCUS
卷 3, 期 3, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsfs.2012.0092
关键词
surface-enhanced Raman scattering; surface-enhanced Raman scattering tag; dimer; hot spot; cancer cell; imaging
类别
资金
- NIH [DP1 OD000798]
- NCI [R01 CA13852701]
- NSF [ECS-0335765]
Surface-enhanced Raman scattering (SERS) tags have been actively explored as a multiplexing platform for sensitive detection of biomolecules. Here, we report a new type of SERS tags that was fabricated by sequentially functionalizing dimers made of 50 nm Ag nanospheres with 4-mercaptobenzoic acid as the Raman reporter molecule, silica coating as a protective shell and antibody as a targeting ligand. These dimer-based tags give highly enhanced and reproducible Raman signals owing to the presence of a well-defined SERS hot spot at the junction between two Ag nanospheres in the dimer. The SERS enhancement factor (EF) of an individual dimer tag supported on a glass slide can reach a level as high as 4.3 x 10(6). In comparison, the EFs dropped to 2.8 x 10(5) and 8.7 x 10(5), respectively, when Ag nanospheres and nanocubes with sizes similar to the spheres in the dimer were used to fabricate the tags using similar procedures. The SERS signals from aqueous suspensions of the dimer-based tags also showed high intensity and good stability. Potential use of the dimer-based tags was demonstrated by imaging cancer cells overexpressing HER2 receptors with good specificity and high sensitivity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据