Review
Neurosciences
Qing Liu, Yang Wu, Huadong Wang, Fan Jia, Fuqiang Xu
Summary: This review summarizes the recent advancements in viral tools for neural circuit tracing, discusses the key principles of using viral tools in neuroscience research, and highlights innovations in developing and optimizing viral tools for neural circuit tracing across diverse animal species, including nonhuman primates.
NEUROSCIENCE BULLETIN
(2022)
Article
Biology
Ayumu Inutsuka, Sho Maejima, Hiroyuki Mizoguchi, Ryosuke Kaneko, Rei Nomura, Keiko Takanami, Hirotaka Sakamoto, Tatsushi Onaka
Summary: Transgenic animals expressing fluorescent proteins are commonly used to label specific cells and proteins. In this study, a new Cre recombinase dependent on red fluorescent protein (RFP) was created, allowing selective targeting and tracing of RFP-dependent gene expression in the mouse and rat brain.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Feng Xiong, Hong Yang, Yi-Ge Song, Hai-Bin Qin, Qing-Yang Zhang, Xian Huang, Wei Jing, Manfei Deng, Yang Liu, Zhixiang Liu, Yin Shen, Yunyun Han, Youming Lu, Xiangmin Xu, Todd C. Holmes, Minmin Luo, Fei Zhao, Min-Hua Luo, Wen-Bo Zeng
Summary: The H129(Amp) tracer system is a powerful tool for tracing neuronal connectomes. It allows for fast and bright monosynaptic tracing with low toxicity through the use of an HSV amplicon-based system.
NATURE COMMUNICATIONS
(2022)
Article
Anatomy & Morphology
Edith Sanchez-Jaramillo, Gabor Wittmann, Judit Menyhert, Praful Singru, Gabriela B. Gomez-Gonzalez, Eduardo Sanchez-Islas, Nashiely Yanez-Recendis, Jaime Arturo Pimentel-Cabrera, Martha Leon-Olea, Balazs Gereben, Csaba Fekete, Jean-Louis Charli, Ronald M. Lechan
Summary: This study reveals the role of hypothalamic TRH neurons in regulating energy homeostasis through their projection to the tuberomammillary nucleus, which may be mediated by influencing histamine neurons.
BRAIN STRUCTURE & FUNCTION
(2022)
Article
Neurosciences
Matthias S. Krockenberger, Tatianna O. Saleh-Mattesich, Henry C. Evrard
Summary: This study examined the spatial relationship between different architectonic subareas in the insula and found the presence of dysgranular architectonic stripes. This has significant implications for understanding interoceptive processing in humans and other primates.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Biology
Jyoti Tiwari, Sayantan Sur, Anupama Yadav, Raj Kumar, Niraj Rai, Sangeeta Rani, Shalie Malik
Summary: The study investigated the molecular regulation of sleep in migratory redheaded buntings. It was found that during migration, there was increased night-time activity and reduced daytime activity. The recordings showed increased night-time active wakefulness with reduced sleep during migratory phase. Gene expression studies revealed increased expression of glucocorticoid receptor and pro-inflammatory cytokines in pre-migratory and migratory states, and upregulation of Ca2+/calmodulin-dependent protein kinase 2 in the migratory state. Melatonin receptors were increased in the pre-migratory state, while growth hormone-releasing hormone was reduced during migration.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Endocrinology & Metabolism
Marc Schneeberger, Nicola L. Brice, Kyle Pellegrino, Luca Parolari, Jordan T. Shaked, Keith J. Page, Francois Marchildon, Douglas W. Barrows, Thomas S. Carroll, Thomas Tolpiko, Victoria M. Mulligan, Robert Newman, Kevin Doyle, Roland Burli, Daniel F. Barker, Angela Glen, Maria Jose Ortuno, Alexander R. Nectow, Nicolas Renier, Paul Cohen, Mark Carlton, Nathaniel Heintz, Jeffrey M. Friedman
Summary: Food intake and body weight are regulated by specific neurons in the brain. Activating glutamatergic neurons in the brainstem can suppress food intake and increase locomotion. These neurons project to the lateral hypothalamus, which is involved in decreasing food intake. Chronic activation of these neurons can reduce weight in leptin-deficient and diet-induced obese mice. The orexin 1 receptor is highly expressed in these neurons and can be targeted with an antagonist to reduce feeding and body weight. This potential therapy for obesity may also apply to humans.
Article
Neurosciences
Kyle B. Fischer, Hannah K. Collins, Yan Pang, Dheeraj S. Roy, Ying Zhang, Guoping Feng, Shu-Jing Li, Adam Kepecs, Edward M. Callaway
Summary: Identification of synaptic partners is a fundamental task, but reliable techniques for whole brain labeling of downstream partners in a cell-type-dependent and monosynaptic manner are lacking. In this study, the authors developed a novel anterograde tracing system based on genetic manipulation of a virus, resulting in reliable labeling of postsynaptic partners in a brain-wide fashion. This system provides a valuable tool for studying neural circuits.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Anatomy & Morphology
Fillan S. Grady, Shantelle A. Graff, Georgina M. Aldridge, Joel C. Geerling
Summary: This study presents a pipeline for labeling synaptic output of neurons using genetic tracing methods and introduces a detector called BoutonNet to automatically identify labeled presynaptic boutons. The technique achieves high sensitivity and low background noise, enabling quantitative analysis of the fundamental unit of the brain on a whole-brain scale.
BRAIN STRUCTURE & FUNCTION
(2022)
Article
Neurosciences
Coralie Ebert, Knarik Bagdasarian, Sebastian Haidarliu, Ehud Ahissar, Avner Wallach
Summary: This study used artificial whisking in rats to compare the encoding of proprioceptive whisking and exteroceptive touch signals in various brainstem nuclei. The findings show that different nuclei have different neuronal encoding patterns, with Pa5 nucleus containing a complex combination of whisking and touch information, including a significant fraction of neurons inhibited by active touch, a response previously only observed in the thalamus.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Peripheral Vascular Disease
Darius A. Gerlach, Jorge Manuel, Alex Hoff, Hendrik Kronsbein, Fabian Hoffmann, Karsten Heusser, Heimo Ehmke, Jens Jordan, Jens Tank, Florian Beissner
Summary: The study utilized high-resolution functional magnetic resonance imaging to reveal the hypothalamic and medullary responses to acute hypoxia in healthy men, providing insights into the central pathways integrating chemoreflexes and the potential for studying cardiovascular disease and responses to chemoreceptor modulation.
Article
Behavioral Sciences
Jane J. Bartonjo, Robert F. Lundy
Summary: The study found that somatostatin neurons in the central nucleus of the amygdala can project to the nucleus of the solitary tract, parabrachial nucleus, or lateral hypothalamus, indicating a highly heterogeneous population in terms of synaptic targets and likely functions.
Article
Neurosciences
Safiye Cavdar, Busra Kose, Damlasu Altinoz, Mazhar Ozkan, Yasin Celal Gunes, Oktay Algin
Summary: This study investigates the afferent and efferent connections of the supplementary motor area (SMA) with brainstem structures and clarifies its relationship with the corticospinal tract (CST). The results show that the SMA has both afferent and efferent connections with various brainstem structures, which contribute to its motor and non-motor functions. These findings provide new insights into the role of the SMA in brainstem networks.
NEUROSCIENCE LETTERS
(2023)
Article
Clinical Neurology
Jeryn Chang, Thomas B. Shaw, Cory J. Holdom, Pamela A. McCombe, Robert D. Henderson, Jurgen Fripp, Markus Barth, Christine C. Guo, Shyuan T. Ngo, Frederik J. Steyn
Summary: This study found that lower hypothalamic volume is associated with lower and higher BMI in ALS patients, unlike AD patients and controls. Hypothalamic volume is not related to loss of appetite or hypermetabolism. In ALS patients, lower hypothalamic volume with lower BMI is associated with weight loss and earlier death.
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Review
Veterinary Sciences
Ai Namioka, Takahiro Namioka, Masanori Sasaki, Jeffery D. Kocsis, Osamu Honmou
Summary: The study developed a new focal basilar artery occlusion model in rats that causes specific stroke symptoms. This procedure is relatively stable, offers higher survival rates and extended observation periods compared to other brainstem ischemia models.
Article
Endocrinology & Metabolism
Melinda Cservenak, David Keller, Viktor Kis, Emese A. Fazekas, Hanna Oelloes, Andras H. Leko, Eva R. Szabo, Eva Renner, Ted B. Usdin, Miklos Palkovits, Arpad Dobolyi
Article
Neurosciences
Janos Barna, Eva Renner, Antonia Arszovszki, Melinda Cservenak, Zsolt Kovacs, Miklos Palkovits, Arpad Dobolyi
NUTRITIONAL NEUROSCIENCE
(2018)
Review
Neurosciences
Julianna Kardos, Laszlo Heja, Katalin Jemnitz, Richard Kovacs, Miklos Palkovits
PROGRESS IN NEUROBIOLOGY
(2017)
Article
Chemistry, Analytical
Georgi Shilyashki, Helmut Pfuetzner, Martin Palkovits, Andreas Windischhofer, Markus Giefing
Review
Biochemistry & Molecular Biology
Dasiel O. Borroto-Escuela, Manuel Narvaez, Patrizia Ambrogini, Luca Ferraro, Ismel Brito, Wilber Romero-Fernandez, Yuniesky Andrade-Talavera, Antonio Flores-Burgess, Carmelo Millon, Belen Gago, Jose Angel Narvaez, Yuji Odagaki, Miklos Palkovits, Zaida Diaz-Cabiale, Kjell Fuxe
Article
Immunology
Nellie A. Martin, Viktor Molnar, Gabor T. Szilagyi, Maria L. Elkjaer, Arkadiusz Nawrocki, Justyna Okarmus, Agnieszka Wlodarczyk, Eva K. Thygesen, Miklos Palkovits, Ferenc Gallyas, Martin R. Larsen, Hans Lassmann, Eirikur Benedikz, Trevor Owens, Asa F. Svenningsen, Zsolt Illes
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Cell Biology
Adrienn Palvolgyi, James Simpson, Ibolya Bodnar, Judit Biro, Miklos Palkovits, Tamas Radovits, Paul Skehel, Ferenc A. Antoni
CELLULAR SIGNALLING
(2018)
Article
Multidisciplinary Sciences
Nellie A. Martin, Arkadiusz Nawrocki, Viktor Molnar, Maria L. Elkjaer, Eva K. Thygesen, Miklos Palkovits, Peter Acs, Tobias Sejbaek, Helle H. Nielsen, Zoltan Hegedus, Finn Sellebjerg, Tihamer Molnar, Eudes G. Barbosa, Nicolas Alcaraz, Ferenc Gallyas, Asa F. Svenningsen, Jan Baumbach, Hans Lassmann, Martin R. Larsen, Zsolt Illes
Article
Biochemistry & Molecular Biology
Fanni Dora, Eva Renner, David Keller, Miklos Palkovits, Arpad Dobolyi
Summary: This study revealed extensive gene expressional alterations in the dorsomedial prefrontal cortex (DMPFC) related to suicidal behavior. Genes associated with depressive disorder, schizophrenia, and impaired cognition were found to be overexpressed, while pathways related to cytokine receptor signaling were downregulated and glutamatergic synaptic signaling were upregulated in suicide victims.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eva Renner, Fanni Dora, Erzsebet Oszwald, Arpad Dobolyi, Miklos Palkovits
Summary: GLP-1 activates GLP-1R in the PVNmc, and the expression level of GLP-1R in the PVNmc is elevated in T2DM patients. GLP-1 fiber terminals are closely apposed to oxytocin neurons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Bobana Samardzija, Maja Jukovic, Beti Zaharija, Eva Renner, Miklos Palkovits, Nicholas J. Bradshaw
Summary: The study investigates the aggregation of proteins in the brains of individuals with mental illness. Multiple proteins, including CRMP1, DISC1, NPAS3, and TRIOBP-1, are found to form aggregates, but it is unclear whether they aggregate independently or interact with each other. The results show that while some proteins can co-aggregate, it is more common for them to aggregate in a parallel manner through independent mechanisms.
Meeting Abstract
Clinical Neurology
N. A. Martin, V. Molnar, G. T. Szilagyi, M. L. Elkjaer, A. Nawrocki, J. Okarmus, A. Wlodarczyk, E. K. Thygesen, M. Palkovits, F. Gallyas, M. R. Larsen, H. Lassmann, E. Benedikz, T. Owens, A. F. Svenningsen, Z. Illes
MULTIPLE SCLEROSIS JOURNAL
(2017)
Meeting Abstract
Clinical Neurology
N. A. Martin, A. Nawrocki, V. Molnar, M. L. Elkjaer, E. K. Thygesen, M. Palkovits, P. Acs, T. Sejbaek, H. H. Nielsen, Z. Hegedus, F. Sellebjerg, E. G. V. Barbosa, N. Alcaraz, F. Gallyas, A. F. Svenningsen, J. Baumbach, H. Lassmann, M. R. Larsen, Z. Illes
MULTIPLE SCLEROSIS JOURNAL
(2017)
Article
Clinical Neurology
Monika Albert, Alonso Barrantes-Freer, Melanie Lohrberg, Jack P. Antel, John W. Prineas, Miklos Palkovits, Joachim R. Wolff, Wolfgang Brueck, Christine Stadelmann
Meeting Abstract
Neurosciences
Yogesh Dwivedi, Bhaskar Roy, Qingzhong Wang, Miklos Palkovits, Gabor Faludi
BIOLOGICAL PSYCHIATRY
(2017)