Review
Pharmacology & Pharmacy
Patrick M. Perrigue, Richard A. Murray, Angelika Mielcarek, Agata Henschke, Sergio E. Moya
Summary: Nanoformulations offer advantages in enhancing drug solubility, biocompatibility, and bioavailability, reducing side effects. Despite limited research on stability and degradation of nanocarriers in biological environments, techniques exist for tracing the fate of nanocarriers, including their degradation and drug release intra- and extracellularly. Fluorescence techniques and nuclear imaging are commonly used to track nanocarriers in research on nanoformulations.
Article
Pharmacology & Pharmacy
Franziska M. Juergens, Fabian C. Herrmann, Sara M. Robledo, Thomas J. Schmidt
Summary: Arnica tincture is a traditional herbal medicine used for treating blunt injuries and is currently being investigated for its potential use in the treatment of cutaneous leishmaniasis. This study provides detailed information on the dermal absorption of Arnica tincture, particularly its bioactive constituents called sesquiterpene lactones (STLs). The results show that within 48 hours, a majority of the STLs from Arnica tincture were absorbed into the skin, but a significant portion could not be extracted from the skin and were irreversibly bound to skin proteins. Additionally, fluorescence microscopic images revealed the accumulation of a fluorescent derivative of STLs in the epidermis.
Article
Pharmacology & Pharmacy
Thirapit Subongkot, Natthan Charernsriwilaiwat, Rattathammanoon Chanasongkram, Kantawat Rittem, Tanasait Ngawhirunpat, Praneet Opanasopit
Summary: This study aimed to develop microemulsions using poloxamer 124 as a surfactant to improve the skin penetration of finasteride. The microemulsions were found to significantly increase the skin penetration of finasteride, mainly through the hair follicle pathway.
Review
Chemistry, Analytical
S. Bielfeldt, F. Bonnier, H. J. Byrne, I. Chourpa, Y. Dancik
Summary: The study of human skin is significant in various fields including dermatology, toxicology, pharmacology, and cosmetology. Current measurement methods do not provide chemically specific results regarding skin penetration and permeation. However, Raman microspectroscopy can serve as an alternative method for non-destructive, non-invasive, and chemically specific investigations.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Ankan Kumar Sarkar, Santanu Shaw, Himali Arora, Pankaj Seth, Nikhil R. R. Jana
Summary: Subcellular targeting of drugs can be enhanced by designing nanocarriers for direct cell membrane translocation, bypassing endosomal/lysosomal trafficking. This approach allows rapid and extensive delivery of drugs to the cell nucleus within a few minutes, without undergoing vesicular coating and lysosomal trafficking. This nonendocytic uptake method provides a potential strategy for nanocarrier-based subcellular targeting of drugs for more effective therapy.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Yanan Wang, Ke Wu, Shujing Li, Xiaozhen Li, Yifan He
Summary: Encapsulating glabridin with liposome technology can improve its bioavailability and application in cosmetics. Confocal Raman spectroscopy can be used to monitor dermal penetration of cosmetic actives. In this study, CRS was used to evaluate the penetration of glabridin and liposome components. The research demonstrated that liposome-encapsulated glabridin has good skin permeability and a gradual release effect, enhancing the dermal absorption and bioavailability of glabridin.
VIBRATIONAL SPECTROSCOPY
(2023)
Article
Engineering, Biomedical
Tijana Maric, Amalie Lovind, Zhongyang Zhang, Jiayue Geng, Anja Boisen
Summary: In this study, gold-coated mesoporous silica nanoparticles are developed as nanomotors driven by near-infrared light. These nanomotors can propel themselves through biofilms and disperse them through photothermal effect. The velocities of the nanomotors are found to increase with increasing laser power, and they show excellent capabilities to eradicate Pseudomonas aeruginosa biofilms even under short irradiation time. This study holds great promise for overcoming challenges in bacterial biofilm eradication.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Kiyoon Kwon, Youngmin Hwang, Junyoung Jung, Giyoong Tae
Summary: Mixing with adenosine tetraphosphate (ATP) greatly enhances the transport and permeation of a polymeric nanocarrier across the retina via intravitreal administration, showing potential for a new method of drug delivery to the retina.
Article
Engineering, Environmental
Wei Gao, Yongfeng Lin, Yong Liang, Yingjun Wang, Lu Jiang, Yawei Wang, Guibin Jiang
Summary: The experiment revealed that the penetration ability of CPs through the skin was correlated with their molecular structure, with short-chain and medium-chain CPs more easily penetrating the skin compared to long-chain CPs. Additionally, the study suggests that human dermal exposure to CPs could be a significant pathway for CPs intake in the environment, highlighting the importance of further research in this area.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Moritz Radbruch, Hannah Pischon, Fang Du, Rainer Haag, Fabian Schumacher, Burkhard Kleuser, Lars Mundhenk, Achim D. Gruber
Summary: The polymeric core-multishell nanocarrier (CMS) shows promise as a carrier for topical delivery of tacrolimus (TAC). However, in vivo, it does not increase TAC penetration into the skin compared to ointment. Nevertheless, the therapeutic efficacy of TAC delivered by CMS is equivalent to standard TAC ointment.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Pharmacology & Pharmacy
Sabrina Wiemann, Cornelia M. Keck
Summary: This study systematically investigated the influence of dispersed particles within a topical formulation on the dermal penetration efficacy of active compounds dissolved in the water phase. The results demonstrated that the addition of particles significantly increased the dermal penetration efficacy, whereas skin hydrating excipients had limited effects.
Article
Instruments & Instrumentation
Chin-Ping Kung, Bruno C. Sil, Yanling Zhang, Jonathan Hadgraft, Majella E. Lane, Bhumik Patel, Renee McCulloch
Summary: Amitriptyline, currently used for neuropathic pain and migraine, is being explored as a potential topical analgesic. Initial studies suggest that formulations containing isopropyl myristate (IPM) and isopropyl alcohol (IPA) promote higher permeation of amitriptyline through the skin, while those containing propylene glycol (PG) also show promise for dermal delivery. Further optimization is needed for clinical investigational studies.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Keerthana Rajes, Karolina A. Walker, Sabrina Hadam, Fatemeh Zabihi, Fiorenza Rancan, Annika Vogt, Rainer Haag
Summary: A synthetic route for redox-sensitive and non-sensitive core multi-shell (CMS) carriers with sizes below 20 nm was established. The study demonstrated the reducibility and drug release effect of reduction-sensitive CMS nanocarrier, showing significant release of model dye Nile red within 24 hours. Results also showed that the redox-sensitive CMS nanocarrier had higher drug release efficiency and encapsulation performance in ex vivo human skin experiments.
Article
Medicine, Research & Experimental
Hazel Garvie-Cook, Magdalena Hoppel, Richard H. Guy
Summary: This article illustrates the application of confocal Raman microspectroscopy and imaging to investigate the performance of topical and transdermal drug products at the formulation-skin interface. These techniques provide qualitative and potentially semi-quantitative insights into the interaction between the delivery system and the skin, drug transfer mechanisms, changes in drug solubility, and drug absorption and clearance within the skin.
MOLECULAR PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Jamal Alyoussef Alkrad, Reinhard H. H. Neubert
Summary: In this study, insulin was successfully loaded into nano-sized non-ionic colloidal carrier systems for transdermal drug delivery. The CCS exhibited excellent rheological properties and drug release rate. The insulin-loaded CCS showed high transdermal flux through rat's skin and epidermis, and induced a delayed therapeutic effect.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Eszter L. Kiss, Szilvia Berko, Attila Gacsi, Anita Kovacs, Gabor Katona, Judit Soos, Erzsebet Csanyi, Ilona Grof, Andras Harazin, Maria A. Deli, Gyorgy T. Balogh, Maria Budai-Szucs
Article
Pharmacology & Pharmacy
Anita Kovacs, Dora Peter-Hederi, Katalin Perei, Maria Budai-Szucs, Attila Leber, Attila Gacsi, Erzsebet Csanyi, Szilvia Berko
Article
Respiratory System
Maria Budai-Szucs, Szilvia Berko, Anita Kovacs, Pongsiri Jaikumpun, Rita Ambrus, Adrien Halasz, Piroska Szabo-Revesz, Erzsebet Csanyi, Akos Zsembery
Summary: This study investigated the effect of hypertonic salt solutions on CF sputum viscoelasticity in vitro, using a rheometer to measure the rheological properties of samples. The results showed significant changes in gel strength when different additives were used at varying ratios, with NaHCO3 having the most pronounced effect. The study highlights the importance of individual evaluation of sputum samples based on their rheological properties for effective treatment.
BMC PULMONARY MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Lu Cui, Judit Rebeka Molnar, Maria Budai-Szucs, Maria Szecsenyi, Katalin Burian, Peter Valyi, Szilvia Berko, Bela Pukanszky
Summary: The study found that the composition of fibers changes during preparation, with limited solubility of the drug and components leading to formation of multiple phases and drug precipitation. Drug release occurs in a burst-like step, with Amox located among the fibers exhibiting antimicrobial activity.
Article
Pharmacology & Pharmacy
Anita Kovacs, Stella Zsiko, Fanni Falusi, Erzsebet Csanyi, Maria Budai-Szucs, Ildiko Csoka, Szilvia Berko
Summary: This study compared permeation across innovative synthetic membranes and human epidermis, with the Strat-M membrane showing the greatest similarity to HSE. Good correlations were found between the permeation data of different formulations across different membranes, allowing the prediction of permeation across HSE using synthetic membranes. Strat-M and Skin PAMPA membranes have the potential to select and differentiate dermal formulations containing diclofenac sodium as an early screening model.
Article
Pharmacology & Pharmacy
Fanni Falusi, Maria Budai-Szucs, Erzsebet Csanyi, Szilvia Berko, Tamas Spaits, Ildiko Csoka, Anita Kovacs
Summary: Dermal foams are promising drug delivery systems for treating skin conditions, and the QbD approach plays a crucial role in ensuring their quality during development. Different types of polymers have varying effects on foam properties, with polymer content being a key factor.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Nikolett Kis, Maria Gunnarsson, Szilvia Berko, Emma Sparr
Summary: The skin provides an attractive alternative to conventional drug administration routes, but the stratum corneum (SC) presents challenges as an efficient barrier to most compounds. This study investigates the molecular effects of three types of glycols in the SC, aiming to understand their influence on molecular mobility and structure, as well as their efficiency as permeation enhancers. The results show that all three glycols increase lipid mobility in the SC and have permeation enhancing effects. There is also a non-monotonic response in permeation enhancement with respect to glycol concentration.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Pharmacology & Pharmacy
Nikolett Kis, Anita Kovacs, Maria Budai-Szucs, Gabor Eros, Erzsebet Csanyi, Szilvia Berko
Summary: This study investigated the effect of non-invasive dermal electroporation (EP) on the barrier function of the skin and the permeation of a macromolecule. The results showed that EP can temporarily decrease the skin's barrier function and the structure of the skin is restored shortly after treatment. EP significantly increased the permeation rate of the macromolecule, but the composition of the formulations had a great influence on the permeation.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Reka Szoleczky, Maria Budai-Szucs, Erzsebet Csanyi, Szilvia Berko, Peter Tonka-Nagy, Ildiko Csoka, Anita Kovacs
Summary: The aim of this study was to design an effective in vitro release test method using USP apparatus IV with a semi-solid adapter for diclofenac sodium hydrogel, by applying the analytical quality by design approach. The analytical target profile and critical method attributes were defined, and an initial risk assessment was conducted. High-risk parameters were identified and a test protocol was developed for the hydrogel system.
Article
Chemistry, Medicinal
Szabolcs Laszlo, Istvan Z. Batai, Szilvia Berko, Erzsebet Csanyi, Agnes Dombi, Gabor Pozsgai, Kata Bolcskei, Lajos Botz, Odon Wagner, Erika Pinter
Summary: In this study, the release properties, skin penetration, and analgesic effect of a modified silicone-polymer-based transdermal therapeutic system (TTS) loaded with low-dose capsaicin were investigated. The TTS exhibited controlled, zero-order kinetic release of capsaicin and effectively penetrated into the epidermis and dermis of human skin. It was concluded that this modified TTS is suitable for relieving traumatic and inflammatory pain.
Article
Pharmacology & Pharmacy
Diana Balogh-Weiser, Alexandra Molnar, Gergo D. Toth, Gabor Koplanyi, Jozsef Szemes, Balazs Decsi, Gabor Katona, Maryana Salamah, Ferenc Ender, Anita Kovacs, Szilvia Berko, Maria Budai-Szucs, Gyorgy T. Balogh
Summary: The application of enzyme-based therapies has gained attention in drug development. This study developed polymeric nanofibrous matrices to entrap lipases and nadifloxacin for topical skin treatment. The electrospinning process significantly enhanced the enzyme activity of lipases and facilitated the delivery of nadifloxacin to the human epidermis.
Review
Biochemistry & Molecular Biology
Rabia Ashfaq, Akhtar Rasul, Sajid Asghar, Anita Kovacs, Szilvia Berko, Maria Budai-Szucs
Summary: Nano-scale bioactive colloidal carrier systems, such as lipid nanoparticles (LNPs), have shown great potential in overcoming challenges associated with disease treatments. LNPs not only improve the pharmacokinetics and stability of encapsulated compounds, but also provide efficient targeting and reduced toxicity. However, the poor solubility, stability, and absorption of natural therapeutic molecules have hindered research in the nutraceutical sector. Loading these molecules into LNPs offers a promising solution by utilizing their ability to accommodate both hydrophilic and hydrophobic molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Boglarka Szalai, Orsolya Jojart-Laczkovich, Anita Kovacs, Szilvia Berko, Gyorgy Tibor Balogh, Gabor Katona, Maria Budai-Szucs
Summary: This study aims to develop in situ gelling mucoadhesive ophthalmic preparations that can improve the bioavailability of eye drops. The results show that formulations based on thermosensitive polymers and mucoadhesive polymers can increase the residence time and penetration of the active pharmaceutical ingredient, thereby enhancing the bioavailability of the eye drops.
Article
Polymer Science
Fanni Falusi, Szilvia Berko, Anita Kovacs, Maria Budai-Szucs
Summary: Foams are becoming increasingly popular in dermatology due to their advantages in terms of easy spreading, good skin sensation, and applicability in special skin conditions. This study aimed to improve the stability of dermal foams by using different hydrogel-forming polymers. The combination of xanthan gum and dexpanthenol was found to be ideal for both foam formation and stability.