期刊
CONSERVATION GENETICS RESOURCES
卷 6, 期 2, 页码 341-344出版社
SPRINGER
DOI: 10.1007/s12686-013-0089-0
关键词
Microsatellite; Saccharina japonica; Saccharina longissima; Next generation DNA sequencing
资金
- National High-Tech R&D Program of China [2012AA10A406]
- National Key Technology R&D Support Program of China [2012BAD55G01]
Twenty-seven polymorphic trinucleotide microsatellite DNA markers were developed from Saccharina japonica, through paired-end Illumina sequencing data. Sixty-three gametophyte clones of the species, preserved indoor as germplasm resources were genotyped using these markers. The number of alleles ranged from 2 to 7 with an average 4.7 per locus. The gene diversity and expected heterozygosity varied from 0.171 to 0.774 and from 0.174 to 0.793, respectively. Sixteen loci could be successfully amplified in Saccharina longissima with 12 loci being polymorphic. These novel microsatellite DNA markers will be extremely useful in germplasm resource conservation and management of S. japonica and also in its related species S. longissima.
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