Microsatellite markers for the West Indian fruit fly (Anastrepha obliqua) and cross species amplification in related pest species

We isolated thirteen polymorphic microsatellite loci from Anastrepha obliqua using a dual-suppression polymerase chain reaction (PCR) technique. These loci provided microsatellite markers with polymorphism of two to eight alleles per locus within different Mexican populations. The expected and observed heterozygosities ranged from 0.125 to 0.820 and 0.095 to 0.783, respectively. These markers may be effective for analyzing population genetics and related study of A. obliqua. Cross-species amplification was noticed with ten out of thirteen microsatellite loci in four closely rated Anastrepha species.