Ten novel microsatellite markers for the western mosquitofish Gambusia affinis

We isolated and characterized 10 microsatellite loci from the western mosquitofish, Gambusia affinis. Loci were screened in 30 individuals from a single location from their native range in coastal Texas. The number of alleles per locus ranged from 4 to 25, observed heterozygosity ranged from 0.40 to 0.93, and the probability of identity values from 6.0 x 10(-3) to 2.7 x 10(-1), with an overall PI for all loci of 1.3 x 10(-13). These new loci provide tools for examining the genetic diversity, structure, and pedigree G. affinis populations.