Review
Oncology
Sharia Hernandez, Rossana Lazcano, Alejandra Serrano, Steven Powell, Larissa Kostousov, Jay Mehta, Khaja Khan, Wei Lu, Luisa M. Solis
Summary: Characterization of the tumor microenvironment through immunoprofiling is vital for understanding immune cell interactions and predicting immunotherapy response. The nanoString GeoMx (R) Digital Spatial Profiler (DSP) allows highplex profiling at the protein and RNA level, offering spatial and temporal assessment of limited tissue samples. This review discusses the technical details, advantages, and limitations of this platform and provides important considerations for translational immune-oncology research.
FRONTIERS IN ONCOLOGY
(2022)
Article
Gastroenterology & Hepatology
Korsuk Sirinukunwattana, Enric Domingo, Susan D. Richman, Keara L. Redmond, Andrew Blake, Clare Verrill, Simon J. Leedham, Aikaterini Chatzipli, Claire Hardy, Celina M. Whalley, Chieh-Hsi Wu, Andrew D. Beggs, Ultan McDermott, Philip D. Dunne, Angela Meade, Steven M. Walker, Graeme Murray, Leslie Samuel, Matthew Seymour, Ian Tomlinson, Phil Quirke, Timothy Maughan, Jens Rittscher, Viktor H. Koelzer
Summary: This study utilized deep learning to analyze histological images of colorectal cancer tissue samples and successfully predicted consensus molecular subtypes, addressing the issue of molecular classification. The image-based classification method accurately identified samples that were unclassifiable by RNA expression analysis, showing potential for biological stratification.
Review
Multidisciplinary Sciences
Jose M. Ayuso, Maria Virumbrales-Munoz, Joshua M. Lang, David J. Beebe
Summary: Precision oncology plays a significant role in patient treatment. Microfluidic systems have potential applications in cancer precision medicine. However, there are challenges that need to be addressed before these technologies can be implemented in the clinic.
NATURE COMMUNICATIONS
(2022)
Review
Oncology
Mohammad Amin Kerachian, Marjan Azghandi, Sina Mozaffari-Jovin, Alain R. Thierry
Summary: Methylation analysis of circulating cell-free DNA (cirDNA) has significant potential in advancing cancer detection, prognosis, and treatment, as well as genetic disorders. The choice of pre-analytical variables can impact cirDNA methylation identification, while different techniques yield varying results. Despite efforts to simplify methylation analysis with new technologies, the process remains complex and lacks a standard method for pre-analytical processing of methylated cirDNA.
CLINICAL EPIGENETICS
(2021)
Review
Oncology
Deborah Plana, Adam C. Palmer, Peter K. Sorger
Summary: Combination therapies are more effective in treating cancer than monotherapy, addressing tumor heterogeneity. The model of independent drug action provides multiple opportunities for benefit from at least one drug, and personalized, targeted combination therapy can increase therapeutic benefits.
Review
Cell Biology
Ulrike Pfohl, Alina Pflaume, Manuela Regenbrecht, Sabine Finkler, Quirin Graf Adelmann, Christoph Reinhard, Christian R. A. Regenbrecht, Lena Wedeken
Summary: Cancer is a multifactorial disease with increasing incidence, posing a huge challenge to treatment. Tumor heterogeneity demands personalized therapy, and precision oncology is evolving beyond genetic variants with the integration of multi-omics technologies.
Review
Biochemistry & Molecular Biology
Gianluca Businello, Valentina Angerilli, Paola Parente, Stefano Realdon, Edoardo Savarino, Fabio Farinati, Federica Grillo, Alessandro Vanoli, Francesca Galuppini, Silvia Paccagnella, Gianmaria Pennelli, Luca Mastracci, Luca Saragoni, Matteo Fassan
Summary: This review describes the landscape of molecular alterations in gastric pre-invasive lesions and discusses their potential use in diagnostic and therapeutic decision-making processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Blake Salfer, Daisy Havo, Skyllar Kuppinger, David T. W. Wong, Feng Li, Liying Zhang
Summary: Liquid biopsy using cell-free DNA (cfDNA) has emerged as a valuable tool for cancer screening and monitoring. While blood-based liquid biopsy has been extensively studied, other body fluids such as saliva offer advantages. Saliva is noninvasive, easily repeatable, and may contain cfDNA from specific types of cancer. However, the lack of standardized pre-analytical procedures for saliva-based testing is a concern. In this study, we assessed pre-analytical variables impacting cfDNA stability in saliva samples. We found that the use of Novosanis's UAS preservative helped stabilize cfDNA in saliva for up to one week at room temperature. Our findings provide valuable insights for the improvement of saliva collection devices and preservation methods.
Article
Medicine, General & Internal
Anum Kazmi, Zia Abbas, Zohra Saleem, Safdar Haider, Waqas Ahmed Farooqui, Shaheen Ahmed
Summary: The objective of this study was to evaluate the levels of salivary matrix metalloproteinases (MMP-8) in oral submucous fibrosis (OSF) and oral squamous cell carcinoma (OSCC) for early stage diagnosis using a non-invasive method. The study found that MMP-8 levels were underexpressed in OSCC and OSF patients compared to controls, indicating an inverse relationship between MMP-8 levels and OSCC and OSF.
Article
Multidisciplinary Sciences
Marlies Gijs, Sinthuja Arumugam, Nienke van de Sande, Carroll A. B. Webers, Swaminathan Sethu, Arkasubhra Ghosh, Rohit Shetty, Jelle Vehof, Rudy M. M. A. Nuijts
Summary: Tear fluid has potential as a non-invasive biomarker source for ocular and systemic conditions, but standardized methods for collecting and processing tear fluid are needed to improve the accuracy of tear protein quantification. This study investigated sample handling factors that may affect tear protein biomarker profiles. Differences in wetting and migration length were observed between Schirmer's strips from different manufacturers and protein-free and protein-rich solutions. The study highlights the importance of standardization in Schirmer's strip manufacturing, tear fluid processing, and analyte concentration normalization.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
William Butler, Lingfan Xu, Yinglu Zhou, Qing Cheng, J. Spencer Hauck, Yiping He, Robert Marek, Zachary Hartman, Liang Cheng, Qing Yang, Mu-En Wang, Ming Chen, Hong Zhang, Andrew J. Armstrong, Jiaoti Huang
Summary: Neuroendocrine (NE) cells represent a small percentage of epithelial cells in the prostate, but they become more abundant in hormonally treated and castration-resistant prostate cancer (CRPC). In some cases, hormonally treated tumors can recur as small cell NE carcinoma (SCNC), which are composed entirely of NE cells. NE cells are resistant to hormonal therapy due to their lack of androgen receptors (ARs). A study has found that glypican-3 (GPC3) is specifically expressed by NE cells in prostate cancer and plays a critical role in their viability and differentiation.
JOURNAL OF PATHOLOGY
(2023)
Article
Oncology
Benjamin Lin, Julia Ziebro, Erin Smithberger, Kasey R. Skinner, Eva Zhao, Timothy F. Cloughesy, Zev A. Binder, Donald M. O'Rourke, David A. Nathanson, Frank B. Furnari, C. Ryan Miller
Summary: This article discusses the unique biology of EGFR in GBM, the challenges in treatment, and how they have influenced past and present EGFR-targeted therapeutic design and clinical trials. It also explores the adjustments needed to exploit EGFR in this disease.
Editorial Material
Oncology
Aakash Desai, Vivek Subbiah
Summary: Immunotherapy has shown benefits in some cancer cases, but most patients still face cancer progression despite treatment. Precision-driven patient selection is proposed to identify successful combinations of immunotherapy for improved outcomes.
Article
Oncology
Nida Zahid, Russell Seth Martins, Wajeeha Zahid, Wardah Khalid, Iqbal Azam, Shireen Shehzad Bhamani, Khabir Ahmad, AdnanAbdul Jabbar, Muhammad Shahzad Shamim, Rashid Jooma Khan, Gohar Javed, Ehsan Bari, Nargis Asad, Syed Ather Enam
Summary: This study found that resilience in brain tumor patients is closely related to familial/household role and working status, and demonstrates the protective effect of resilience on the mental health of brain tumor patients in Pakistan.
Review
Oncology
Manja Meggendorfer, Vaidehi Jobanputra, Kazimierz O. Wrzeszczynski, Paul Roepman, Ewart de Bruijn, Edwin Cuppen, Reinhard Buttner, Carlos Caldas, Sean Grimmond, Charles G. Mullighan, Olivier Elemento, Richard Rosenquist, Anna Schuh, Torsten Haferlach
Summary: Interrogating the tumor genome through whole-genome sequencing (WGS) has the potential to impact cancer diagnostics, prognostication, and therapy selection. This review emphasizes the requirements for implementing, validating, and maintaining a clinical WGS pipeline to obtain high-quality patient-specific data.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Oncology
C. Cruz, M. Castroviejo-Bermejo, S. Gutierrez-Enriquez, A. Llop-Guevara, Y. H. Ibrahim, A. Gris-Oliver, S. Bonache, B. Morancho, A. Bruna, O. M. Rueda, Z. Lai, U. M. Polanska, G. N. Jones, P. Kristel, L. de Bustos, M. Guzman, O. Rodriguez, J. Grueso, G. Montalban, G. Caratu, F. Mancuso, R. Fasani, J. Jimenez, W. J. Howat, B. Dougherty, A. Vivancos, P. Nuciforo, X. Serres-Creixams, I. T. Rubio, A. Oaknin, E. Cadogan, J. C. Barrett, C. Caldas, J. Baselga, C. Saura, J. Cortes, J. Arribas, J. Jonkers, O. Diez, M. J. O'Connor, J. Balmana, V Serra
ANNALS OF ONCOLOGY
(2018)
Meeting Abstract
Oncology
Christos Sotiriou, Francoise Rothe, Marion Maetens, Debora Fumagalli, David Norman Brown, Roberto Salgado, Ian Bradbury, Lajos Pusztai, Nadia Harbeck, Michail Ignatiadis, Severine Sarp, Lorena de la Pena, Evandro de Azambuja, Jens Huober, Paolo Nuciforo, Jose Baselga, Martine Piccart, Sherene Loi, David Venet
Article
Medicine, Research & Experimental
Isabel Puig, Stephan P. Tenbaum, Irene Chicote, Oriol Arques, Jordi Martinez-Quintanilla, Estefania Cuesta-Borras, Lorena Ramirez, Pilar Gonzalo, Atenea Soto, Susana Aguilar, Cristina Eguizabal, Ginevra Caratu, Aleix Prat, Guillem Argiles, Stefania Landolfi, Oriol Casanovas, Violeta Serra, Alberto Villanueva, Alicia G. Arroyo, Luigi Terracciano, Paolo Nuciforo, Joan Seoane, Juan A. Recio, Ana Vivancos, Rodrigo Dienstmann, Josep Tabernero, Hector G. Palmer
JOURNAL OF CLINICAL INVESTIGATION
(2018)
Correction
Multidisciplinary Sciences
Amaia Zabala-Letona, Amaia Arruabarrena-Aristorena, Natalia Martin-Martin, Sonia Fernandez-Ruiz, James D. Sutherland, Michelle Clasquin, Julen Tomas-Cortazar, Jose Jimenez, Ines Torres, Phong Quang, Pilar Ximenez-Embun, Ruzica Bago, Aitziber Ugalde-Olano, Ana Loizaga-Iriarte, Isabel Lacasa-Viscasillas, Miguel Unda, Veronica Torrano, Diana Cabrera, Sebastiaan M. van Liempd, Ylenia Cendon, Elena Castro, Stuart Murray, Ajinkya Revandkar, Andrea Alimonti, Yinan Zhang, Amelia Barnett, Gina Lein, David Pirman, Ana R. Cortazar, Leire Arreal, Ludmila Prudkin, Ianire Astobiza, Lorea Valcarcel-Jimenez, Patricia Zuniga-Garcia, Itziar Fernandez-Dominguez, Marco Piva, Alfredo Caro-Maldonado, Pilar Sanchez-Mosquera, Mireia Castillo-Martin, Violeta Serra, Naiara Beraza, Antonio Gentilella, George Thomas, Mikel Azkargorta, Felix Elortza, Rosa Farras, David Olmos, Alejo Efeyan, Juan Anguita, Javier Munoz, Juan M. Falcon-Perez, Rosa Barrio, Teresa Macarulla, Jose M. Mato, Maria L. Martinez-Chantar, Carlos Cordon-Cardo, Ana M. Aransay, Kevin Marks, Jose Baselga, Josep Tabernero, Paolo Nuciforo, Brendan D. Manning, Katya Marjon, Arkaitz Carracedo
Article
Oncology
Jaume Capdevila, Ignacio Matos, Francesco M. Mancuso, Carmela Iglesias, Paolo Nuciforo, Carles Zafon, Hector G. Palmer, Zighereda Ogbah, Laura Muinos, Jorge Hernando, Guillermo Villacampa, Carol E. Pena, Josep Tabernero, Marcia S. Brose, Martin Schlumberger, Ana Vivancos
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Oncology
Carmine De Angelis, Chandandeep Nagi, Cliff C. Hoyt, Linying Liu, Kristin Roman, Chichung Wang, Yi Zheng, Jamunarani Veeraraghavan, Vidyalakshmi Sethunath, Paolo Nuciforo, Tao Wang, Anna Tsimelzon, Sufeng Mao, Susan G. Hilsenbeck, Meghana V. Trivedi, Maria Letizia Cataldo, Anne Pavlick, Antonio C. Wolff, Britta Weigelt, Jorge S. Reis-Filho, Aleix Prat, Carolina Gutierrez, Charles Kent Osborne, Mothaffar F. Rimawi, Rachel Schiff
CLINICAL CANCER RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Serena Di Cosimo, Valentina Appierto, Sara Pizzamiglio, Marco Silvestri, Jose Baselga, Martine Piccart, Jens Huober, Miguel Izquierdo, Lorena de la Pena, Florentine S. Hilbers, Evandro de Azambuja, Michael Untch, Lajos Pusztai, Kathleen Pritchard, Paolo Nuciforo, Anne Vincent-Salomon, Fraser Symmans, Giovanni Apolone, Filippo G. de Braud, Marilena Iorio, Paolo Verderio, Maria Grazia Daidone
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Fara Braso-Maristany, Gaia Griguolo, Tomas Pascual, Laia Pare, Paolo Nuciforo, Antonio Llombart-Cussac, Begona Bermejo, Mafalda Oliveira, Serafin Morales, Noelia Martinez, Maria Vidal, Barbara Adamo, Olga Martinez, Sonia Pernas, Rafael Lopez, Montserrat Munoz, Nuria Chic, Patricia Galvan, Isabel Garau, Luis Manso, Jesus Alarcon, Eduardo Martinez, Sara Gregorio, Roger R. Gomis, Patricia Villagrasa, Javier Cortes, Eva Ciruelos, Aleix Prat
NATURE COMMUNICATIONS
(2020)
Article
Oncology
Alfonso Garcia-Valverde, Jordi Rosell, Garazi Serna, Claudia Valverde, Joan Carles, Paolo Nuciforo, Jonathan A. Fletcher, Joaquin Arribas, Oliver Politz, Cesar Serrano
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Oncology
Nuria Chic, Stephen J. Luen, Paolo Nuciforo, Roberto Salgado, Debora Fumagalli, Florentine Hilbers, Yingbo Wang, Evandro de Azambuja, Istvan Lang, Serena Di Cosimo, Cristina Saura, Jens Huober, Aleix Prat, Sherene Loi
Summary: The CelTIL score has been identified as a promising biomarker in early-stage HER2-positive breast cancer, showing potential to guide the escalation or deescalation of systemic therapy.
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
(2022)
Article
Oncology
Helena Verdaguer, Tamara Sauri, Daniel Alejandro Acosta, Magdalena Guardiola, Alexandre Sierra, Jorge Hernando, Paulo Nuciforo, Josep M. Miquel, Cristina Molero, Sandra Peiro, Queralt Serra-Camprubi, Guillermo Villacampa, Susana Aguilar, Ana Vivancos, Josep Tabernero, Rodrigo Dienstmann, Teresa Macarulla
Summary: Cholangiocarcinomas are highly heterogeneous at the molecular level. The classification of actionable molecular alterations according to ESCAT is associated with survival in cholangiocarcinoma patients.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Zhen Shi, Julia Wulfkuhle, Malgorzata Nowicka, Rosa Gallagher, Cristina Saura, Paolo G. Nuciforo, Isabel Calvo, Jay Andersen, Jose Luis Passos-Coelho, Miguel J. Gil-Gil, Begona Bermejo, Debra A. Pratt, Eva M. Ciruelos, Patricia Villagrasa, Matthew J. Wongchenko, Emanuel F. Petricoin, Mafalda Oliveira, Steven J. Isakoff
Summary: This study demonstrates the association between high baseline pAKT levels, mutations in PI3K/AKT pathway components, and the enriched benefit of ipatasertib in TNBC.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
P. Nuciforo, T. Pascual, J. Cortes, A. Llombart-Cussac, R. Fasani, L. Pare, M. Oliveira, P. Galvan, N. Martinez, B. Bermejo, M. Vidal, S. Pernas, R. Lopez, M. Munoz, I. Garau, L. Manso, J. Alarcon, E. Martinez, V. Rodrik-Outmezguine, J. C. Brase, P. Villagrasa, A. Prat, E. Holgado
ANNALS OF ONCOLOGY
(2018)
Meeting Abstract
Oncology
R. Fasani, L. Comerma, F. Pagliuca, S. Thyparambil, V. Peg, J. Jimenez, F. Cecchi, T. Hembrough, J. Perez, J. Arribas, J. Cortes, M. Scaltriti, C. Saura, P. Nuciforo
EUROPEAN JOURNAL OF CANCER
(2018)
Meeting Abstract
Oncology
S. Simonetti, N. Dominguez, F. Pepe, M. Nacchio, F. Conticelli, U. Malapelle, L. Sanchez, X. Guardia, L. Insabato, P. Nuciforo, M. A. Carrasco
EUROPEAN JOURNAL OF CANCER
(2018)