期刊
CELL MOTILITY AND THE CYTOSKELETON
卷 65, 期 8, 页码 652-661出版社
WILEY-LISS
DOI: 10.1002/cm.20291
关键词
troponin; vascular smooth muscle; intestinal smooth muscle
类别
资金
- NCI NIH HHS [P01 CA056666, CA56666] Funding Source: Medline
- NHLBI NIH HHS [R01 HL074184, R01 HL057461, HL63926, R29 HL057461, HL57461, HL03985, HL74184, P01 HL063926, P01 HL063926-05] Funding Source: Medline
It is generally believed that proteins of the troponin complex are not expressed in smooth muscle. We have directly assayed for expression of troponin transcripts in mouse vascular smooth muscle and found that troponin sequences normally associated with fast twitch skeletal muscle (fTnT, tTnI, fTnC) were present at significant levels in the thoracic aorta. In situ hybridization experiments demonstrated that fTnT, fTnI and fTnC transcripts were expressed in the smooth muscle layer of mouse blood vessels of all sizes. Protein blot analysis using rat tissue showed that at least two members of the troponin complex, Troponin T and Troponin I, were translated in vascular smooth muscle of the aorta. Finally, immuno-fluorescence microscopy of rat aortic smooth muscle revealed that TnT and TnI are localized in a unique pattern, coincident with the distribution of tropomyosin. It seems likely therefore, that a complete troponin complex is expressed in vascular smooth muscle and is associated with the contractile machinery of the cell. These observations raise the possibility that troponins play a role in regulation of smooth muscle function.
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