期刊
BIOMEDICAL OPTICS EXPRESS
卷 3, 期 9, 页码 1993-2003出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.3.001993
关键词
-
资金
- Army Research Office [W911NF-09-1-0101]
- National Science Foundation [ECCS0746296, CBET0643583]
- Vanderbilt Institute of Nanoscale Science and Engineering under NSF [ARI-R2 DMR-0963361]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [0963361] Funding Source: National Science Foundation
- Div Of Electrical, Commun & Cyber Sys
- Directorate For Engineering [0746296] Funding Source: National Science Foundation
A grating-coupled porous silicon (PSi) waveguide with an integrated polydimethylsiloxane (PDMS) flow cell is demonstrated as a platform for near real-time detection of chemical and biological molecules. This sensor platform not only allows for quantification of molecular binding events, but also provides a means to improve understanding of diffusion and binding mechanisms in constricted nanoscale geometries. Molecular binding events in the waveguide are monitored by angle-resolved reflectance measurements. Diffusion coefficients and adsorption and desorption rate constants of different sized chemical linkers and nucleic acid molecules are determined based on the rate of change of the measured resonance angle. Experimental results show that the diffusion coefficient in PSi is smaller than that in free solutions, and the PSi morphology slows the molecular adsorption rate constant by a factor of 10(2)-10(4) compared to that of flat surface interactions. Calculations based on simplified mass balance equations and COMSOL simulations give good agreement with experimental data. (c) 2012 Optical Society of America
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据