Article
Optics
Jiajia Chen, Zhenhong Du, Chenhao Xu, Xiao Xiao, Wei Gong, Ke Si
Summary: Here we report an ultrafast 3D histological imaging scheme based on optimized selective plane illumination microscopy (mSPIM), a minutes-time scale clearing method (FOCM), and a deep learning-based image enhancement algorithm (SRACNet) to realize histological preparation and imaging of clinical tissues. Our scheme enables fast imaging at micron-level resolution and demonstrates detailed 3D morphological connections that are difficult to identify in conventional 2D histology.
Article
Optics
Alejandro Gimeno-Gomez, Seyedeh Parisa Dajkhosh, Cong Tuan Son Van, Juan Carlos Barreiro, Chrysanthe Preza, Genaro Saavedra
Summary: A new approach for 3D structured illumination microscopy (SIM) based on a quasi-monochromatic extended source illuminating a Wollaston prism has been designed and implemented to improve robustness, light efficiency, and flexibility. The proposed tunable structured illumination microscopy (TSIM) system shows advantages over traditional SIM systems in terms of enhanced optical sectioning capability and improved OTF compact support. The additional tunability of the 3D SI pattern in the TSIM system provides a unique opportunity for OTF engineering.
Article
Automation & Control Systems
Jose Ramirez-Senent, Jaime H. Garcia-Palacios, Ivan M. Diaz
Summary: This article presents the practical application of dynamics inversion techniques to the force control of electrodynamic proof-mass actuators used as active vibration absorption systems. A novel vibration control algorithm called velocity feedback with dynamics inversion is proposed and its performance is evaluated through experimental tests on a glass fiber reinforced polymer footbridge. The results show that the suggested method outperforms the classical velocity feedback approach in reducing vibrations.
CONTROL ENGINEERING PRACTICE
(2023)
Article
Chemistry, Multidisciplinary
Yeon Ui Lee, Clara Posner, Zhaoyu Nie, Junxiang Zhao, Shilong Li, Steven Edward Bopp, Gde Bimananda Mahardika Wisna, Jeongho Ha, Chengyu Song, Jin Zhang, Sui Yang, Xiang Zhang, Zhaowei Liu
Summary: The resolution capability of SIM is crucial in various fields, but achieving resolutions beyond 40 nm at visible frequencies remains a challenge. This study introduces a low-loss natural organic hyperbolic material (OHM) that can support high spatial-frequency modes beyond 50k(0) at visible frequencies. It demonstrates imaging resolution at 30 nm scales with enhanced photo stability, biocompatibility, ease of use, and cost-effectiveness.
Article
Chemistry, Multidisciplinary
Yeon Ui Lee, Zhaoyu Nie, Shilong Li, Charles-Henri Lambert, Junxiang Zhao, Fan Yang, G. Bimananda M. Wisna, Sui Yang, Xiang Zhang, Zhaowei Liu
Summary: Researchers have fabricated high-quality ultrathin layered hyperbolic metamaterials (HMMs) for the use in super-resolution microscopy. By using this ultrathin layered HMM-assisted illumination, they achieved a 14-fold improvement in imaging resolution, which was verified through experiments.
Article
Optics
Xiaomin Zheng, Jie Zhou, Lei Wang, Meiting Wang, Wenshuai Wu, Jiajie Chen, Junle Qu, Bruce Zhi Gao, Yonghong Shao
Summary: Structured Illumination Microscopy (SIM) has shown remarkable progress in resolution, imaging depth, and technological advancements in recent years, making it a powerful tool in biomedical research. Its flexibility allows for effective combination with other methods, offering enhanced imaging capabilities.
Article
Computer Science, Information Systems
Yuichiro Takeuchi, Kunihiko Nagamine
Summary: Integral imaging, commonly used in glasses-free 3D displays, has evolved into integral illumination for programmable lighting effects. By controlling plenoptic light fields, integral illumination devices can replicate real and imaginary light sources with high fidelity for various applications. This technology offers possibilities for ambient lighting, photography/videography, artistic installations, and more, with potential for further improvements and extensions.
Article
Engineering, Chemical
Concepcion Paz, Adrian Cabarcos, Jesus Vence, Christian Gil
Summary: In this paper, an algorithm based on an active contour model is proposed to extract soot projections from uneven illumination TEM imaging. The proposed method involves pre-processing with a median filter and size normalization, and uses a level set formulation with local region fitting for noisy image segmentation. Statistical comparisons and evaluations show that the algorithm can accurately extract soot regions under uneven illumination with excellent agreement.
Article
Nanoscience & Nanotechnology
Ruslan Rohrich, A. Femius Koenderink
Summary: This article introduces a new structured illumination microscopy technique that extends the capabilities of SIM by combining ideas from illumination engineering and nanophotonics. Experimental results demonstrate the potential for resolution enhancements beyond regular SIM, validated through simulations and experiments. However, there are still obstacles to overcome in the implementation of experiments for artifact-free super-resolution imaging.
Article
Optics
Shun Zhou, Jiaji Li, Jiasong Sun, Ning Zhou, Habib Ullah, Zhidong Bai, Qian Chen, Chao Zuo
Summary: Optical diffraction tomography (ODT) is a promising label-free three-dimensional microscopic method for measuring the refractive index distribution of optically transparent samples. Non-interferometric ODT techniques have gained attention, but suffer from low-frequency missing problems in high numerical aperture systems. Researchers have proposed transport-of-intensity Fourier ptychographic diffraction tomography (TI-FPDT) to address this issue. TI-FPDT combines ptychographic angular diversity with transport of intensity measurements to overcome reconstruction quality deterioration and refractive index underestimation in conventional FPDT.
Article
Optics
Tristan C. Paul, Guy Hagen
Summary: A new technique called Spinning Disk Structured Illumination Microscopy (SD-SIM) was investigated, which combines Spinning Disk microscopy with Structured Illumination Microscopy to achieve better background removal and detect cellular structures that were previously undetectable with either method alone.
Article
Multidisciplinary Sciences
Yanquan Mo, Fan Feng, Heng Mao, Junchao Fan, Liangyi Chen
Summary: Despite the wide application of structured illumination microscopy (SIM) in live-cell super-resolution imaging, aberrations caused by various sources remain a challenge. This study focused on investigating the impact of sample thickness on aberrations due to scattering of excitation light in SIM imaging, finding that thicker samples led to more severe aberrant interference light and degraded image reconstruction. The findings can guide future algorithm development to suppress SIM artefacts caused by scattering in thick samples.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
(2021)
Article
Optics
Ankit Butola, Sebastian Acuna, Daniel Henry Hansen, Krishna Agarwal
Summary: We propose a novel approach to achieve super-resolution structured illumination microscopy using an alignment non-critical illumination system and a reconstruction algorithm that does not require illumination information. This method simplifies instrumentation and allows for system adaptability to different resolutions without recalibration.
Article
Optics
Zhijie Hua, Xu Zhang, Dawei Tu
Summary: This paper proposes a high-precision autofocus method with laser split-image (LSA) for microscopic measurement. The LSA method improves focusing accuracy and robustness by actively incorporating image features. A lightweight comparative framework model for LSA, called split-image comparison model (SCM), is introduced to further enhance focusing performance. The experiment shows that LSA outperforms natural-image-based methods, and SCM achieves significant improvements in accuracy and robustness.
Article
Optics
Zhiqiang Fu, Qiang Geng, Jialong Chen, Li-An Chu, Ann-Shyn Chiang, Shih-Chi Chen
Summary: This study introduces a light sheet-based structured light illumination light field microscopy system for fast 3D imaging, utilizing a digital micromirror device to generate sinusoidal patterns rapidly. By combining deconvolution and HiLo algorithms, fast volumetric imaging with improved optical cross-sectioning capability is achieved. Imaging experiments on various samples show that this new method presents a fast 3D imaging solution with significantly enhanced optical cross-sectioning capability compared to standard light sheet-based LFM.