Article
Chemistry, Multidisciplinary
Ning Yang, Wenhao Hui, Sizhe Dong, Xuming Zhang, Liyang Shao, Yanwei Jia, Pui-In Mak, Rui Paulo da Silva Martins
Summary: A method for joint assessment of cell viability and vitality based on ECIS technology and environmental temperature control was proposed, providing accurate evaluation of cell health status. Compared to traditional methods, this approach is simpler, faster, and less dependent on sample conditions.
Article
Biochemistry & Molecular Biology
Chien-Chu Huang, Shao-Chih Chiu, Shih-Chi Chao, Heng-Yi Liao, Shiao-Pieng Lee, Chun-Chung Huang, Der-Yang Cho
Summary: CBD has a cytotoxic effect on oral cancer cells and induces apoptosis. It also affects the morphology and migration of OEC-M1 cells. ECIS analysis shows that CBD decreases the overall resistance and morphological parameters of the cells in a concentration-dependent manner. The study demonstrates that ECIS is more sensitive, functional, and comprehensive than traditional biochemical assays.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Giuseppina Bozzuto, Giuseppe D'Avenio, Maria Condello, Simona Sennato, Ezio Battaglione, Giuseppe Familiari, Agnese Molinari, Mauro Grigioni
Summary: This study compared the use of Electric Cell-substrate Impedance-Sensing (ECIS) with traditional cytotoxicity tests, finding that ECIS can provide continuous and noninvasive monitoring of cell behavior, potentially offering advantages in studying ZnO NPs toxicity. Furthermore, impedance curve trends were able to distinguish between different cell death mechanisms, offering cost and time advantages.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Yu-Sheng Hsiao, Edgar Daniel Quinones, Shih-Chieh Yen, Jiashing Yu, Ji-Tseng Fang, Peilin Chen, Ruey-Shin Juang
Summary: Electric cell-substrate impedance sensing (ECIS) is an innovative approach for the label-free and real-time detection of cell morphology, growth, and apoptosis. In this study, poly(3,4-ethylenedioxythiophene)-poly-(styrenesulfonate) (PEDOT:PSS) conducting polymer (CP)-based bioelectrodes were developed for the simultaneous drug release and real-time biosensing of cancer cell viability. This device can reliably monitor impedance signal changes correlated with cell viability while exhibiting excellent properties for both drug encapsulation and on-demand release.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Amina Ghorbel, Franck M. Andre, Lluis M. Mir, Tomas Garcia-Sanchez
Summary: This study explores a new method to improve the contact between conductive nanoparticles and the cell membrane using the effects of electric pulses. The results demonstrate that delivering an electric field prior to electroporation pulses can increase the accumulation of nanoparticles around the cell membrane, reducing the need for long incubations between cells and nanoparticles to observe enhanced electroporation.
BIOELECTROCHEMISTRY
(2021)
Article
Biophysics
Felix Hempel, Jessica Ka Yan Law, Thanh Chien Nguyen, Ruben Lanche, Anna Susloparova, Xuan Thang Vu, Sven Ingebrandt
Summary: The study investigated single-cell adhesion on organic electrochemical transistors using an impedimetric readout method, demonstrating single-cell resolution and potential future applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Viktoriya Sokolova, Jana-Fabienne Ebel, Sebastian Kollenda, Kai Klein, Benedikt Kruse, Claudia Veltkamp, Christian M. Lange, Astrid M. Westendorf, Matthias Epple
Summary: Ultrasmall gold nanoparticles are capable of penetrating intestinal cells, colorectal cancer cells, and related 3D structures, while dissolved dyes cannot be taken up by these cells or structures. The cellular uptake of ultrasmall gold nanoparticles is found to occur through different endocytosis pathways. The nanoparticles conjugated with the cytostatic drug doxorubicin exhibit enhanced cytotoxicity against tumor spheroids.
Review
Materials Science, Multidisciplinary
Xiu Liang, Ning Li, Runhao Zhang, Penggang Yin, Chenmeng Zhang, Nan Yang, Kang Liang, Biao Kong
Summary: Photochemical detection of bioactive molecules using carbon-based nanomaterials, such as carbon quantum dots, carbon nanotubes, graphene, and carbon-based core-shell nanostructures, has become a rapidly developing field in biomedical and biological engineering. These materials offer high sensitivity, abundant structural information, and potential applications in in situ analysis, quantitative analysis, and flexible photoelectric functional materials.
NPG ASIA MATERIALS
(2021)
Article
Chemistry, Analytical
Jiating Cao, Meiting Xie, Xinyu Gao, Zhuoyong Zhang, Jianguo Wang, Wenjuan Zhou, Weijiang Guan, Chao Lu
Summary: The study explores a charge neutralization strategy using multicharged cationic nanocarriers to efficiently assemble with the pH-sensitive HPTS, leading to improved salt tolerance, satisfactory cell permeability, and dual-emission properties of the resulting nanoprobes. The fluorescence ratio demonstrates a linear response over the pH range of 3.0-9.0, and the probes successfully enable the determination and visualization of lysosomal pH variations in living cells, showing great potential for biosensing and bioimaging applications in living systems.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Yi-Jui Chen, Wen-Huei Chang, Chia-Ying Li, Yi-Chun Chiu, Chih-Chia Huang, Chun-Hung Lin
Summary: The study proposes a novel method for synthesizing a gold nanoparticle monolayer directly on the surface of SU-8, investigating the effects of different polymer surfaces and photoinitiator concentrations on photoreduction ability, as well as the influence of SU-8 initial curing time and reduction repetition times on nanoparticle sizes and densities.
MATERIALS & DESIGN
(2021)
Article
Chemistry, Physical
Hirak Chatterjee, Dorothy Bardhan, Sudip Kumar Pal, Keiji Yanase, Sujit Kumar Ghosh
Summary: A retrospective formalism has been designed through critical condensation of electromagnetic scattering theories, macroscopic mixing rules, and micromechanics at the metal-analyte interface, which can be adopted as a generalized model.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Multidisciplinary Sciences
Olivia L. Rose, Myra Arnold, Cerasela-Zoica Dinu
Summary: This study evaluates the impact of a zeolitic imidazole framework on lung epithelial cells and identifies potential toxicity. Real-time technology is used for comprehensive evaluation, demonstrating the benefits compared to other biochemical assays. The observed changes in cell behavior suggest that adjusting the physicochemical characteristics of MOFs can improve their safety and biomedical applications.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemistry & Molecular Biology
Beatriz Salesa, Patricia Ferrus-Manzano, Alberto Tunon-Molina, Alba Cano-Vicent, Marcelo Assis, Juan Andres, Angel Serrano-Aroca
Summary: Gold nanoparticles (AuNPs) are promising for biomedical applications due to their excellent biosafety. However, their cytotoxicity in human HaCaT cells is time-dependent, and concentrations above 300 μg/mL show toxic activity. Low concentrations of AuNPs do not exhibit significant proliferative or antiviral activity, and gene expression is not modified after exposure to AuNPs for 24 hours. These results indicate that AuNPs are stable inactive materials suitable for biomedical and clinical applications.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Engineering, Electrical & Electronic
D. Mathivanan, K. S. Shalini Devi, G. Sathiyan, Ankit Tyagi, V. A. O. P. da Silva, B. C. Janegitz, Jai Prakash, Raju Kumar Gupta
Summary: This study introduces an electrochemical sensor based on nanocomposites of polypyrrole, graphene oxide, and gold nanoparticles for efficient detection of hydrogen peroxide, showing high sensitivity and a wider linear response range. The sensor exhibited high stability and repeatability in practical applications.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Article
Biochemical Research Methods
Yitao Liang, Lin Ji, Tingting Tu, Shanshan Zhang, Bo Liang, Xuesong Ye
Summary: This study developed an on-site platform to monitor the invasion process of cancer cells, optimized parameters to demonstrate experimental results, indicating that changes in impedance magnitude reflect interactions between cells and the extracellular matrix. Further use of this platform could contribute to research on cell metastasis.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Surgery
Marianne. C. Kalff, Mark I. van Berge Henegouwen, Peter C. Baas, Renu R. Bahadoer, Eric J. T. Belt, Baukje Brattinga, Linda Claassen, Admira Cosovic, David Crull, Freek Daams, Annette. D. D. van Dalsen, Jan Willem T. Dekker, Marc. J. J. van Det, Manon Drost, Peter van Duijvendijk, Wietse. J. J. Eshuis, Stijn van Esser, Marcia. P. P. Gaspersz, Burak Gorgec, Richard P. R. Groenendijk, Henk H. Hartgrink, Erwin van der Harst, Jan Willem Haveman, Joos Heisterkamp, Richard van Hillegersberg, Wendy Kelder, B. Feike Kingma, Willem J. Koemans, Ewout A. Kouwenhoven, Sjoerd M. Lagarde, Frederik Lecot, Philip P. van der Linden, Misha D. P. Luyer, Grard A. P. Nieuwenhuijzen, Pim B. Olthof, Donald L. van der Peet, Jean-Pierre E. N. Pierie, E. G. J. M. Robert Pierik, Victor D. Plat, Fatih Polat, Camiel Rosman, Jelle P. Ruurda, Johanna W. van Sandick, Rene Scheer, Cettela A. M. Slootmans, Meindert N. Sosef, Odin Sosef, Wobbe O. de Steur, Hein B. A. C. Stockmann, Fanny J. Stoop, Daan M. Voeten, Guusje Vugts, Guy H. E. J. Vijgen, Viola B. Weeda, Marinus J. Wiezer, Martijn G. H. van Oijen, Suzanne S. Gisbertz
Summary: This study evaluated the nationwide trends in care and postoperative outcomes for patients with distal esophageal and gastro-esophageal junction cancer. The results showed that there was a transition towards more neo-adjuvant treatment, transthoracic esophagectomy and minimally invasive surgery over a 10-year period, accompanied by improved postoperative outcomes.
Article
Surgery
Hylke J. F. Brenkman, Linda Claassen, Gerjon Hannink, Leonie R. van der Werf, Jelle P. -H. Ruurda, Grard A. P. Nieuwenhuizen, Misha D. P. Luyer, Ewout A. Kouwenhoven, Marc J. van Det, Mark I. van Berge Henegouwen, Suzanne S. Gisbertz, Jan H. M. B. Stoot, Karel W. E. Hulsewe, Frans van Workum, Richard van Hillegersberg, Camiel Rosman
Summary: This study evaluated the learning curve of laparoscopic gastrectomy (LG) in the Netherlands and found that there was no significant learning curve effect for anastomotic leakage, but there was a learning curve effect for overall complications and conversion rate.
Article
Oncology
Merel Hermus, Berend J. van der Wilk, Rebecca T. H. Chang, Gerlise Collee, Bo J. Noordman, Peter-Paul L. O. Coene, Jan Willem T. Dekker, Henk H. Hartgrink, Joos Heisterkamp, Grard A. P. Nieuwenhuijzen, Camiel Rosman, Liesbeth Timmermans, Bas P. L. Wijnhoven, Charlene J. van der Zijden, Jan J. Busschbach, J. Jan B. van Lanschot, Sjoerd M. Lagarde, Leonieke W. Kranenburg
Summary: Active surveillance may be a safe and effective treatment option for patients with oesophageal cancer after neoadjuvant chemoradiotherapy. Patient preferences and coping styles play a role in treatment decisions, and our study provides insights that can aid healthcare professionals in understanding and addressing patient preferences.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Surgery
Henk-Thijs Brandsma, Birgitta ME. Hansson, Theo J. Aufenacker, Nienke de Jong, Klaas CA. Engelenburg, Chander Mahabier, Rogier Donders, Pascal Steenvoorde, Tammo de Vries S. Reilingh, Hendrik Leendert van Westreenen, Marinus J. Wiezer, Johannes H. W. de Wilt, Maroeska Rovers, Camiel Rosman, Dutch Prevent Study Grp
Summary: The aim of this study was to determine the effectiveness, safety, and cost-effectiveness of prophylactic mesh placement in preventing parastomal hernia formation in the long term. The results showed that while the procedure did not reduce the incidence of parastomal hernia after 5 years of follow-up, it did delay its onset without significant differences in morbidity, mortality, or quality of life. Additionally, it was found to be a cost-effective option.
Article
Gastroenterology & Hepatology
Laura F. C. Fransen, Rob H. A. Verhoeven, Thijs H. J. B. Janssen, Marc J. van Det, Suzanne S. Gisbertz, Richard van Hillegersberg, Bastiaan Klarenbeek, Ewout A. Kouwenhoven, Grard A. P. Nieuwenhuijzen, Camiel Rosman, Jelle P. Ruurda, Mark van Berge Henegouwen, Misha D. P. Luyer
Summary: This study assesses the association between post-esophagectomy complications and long-term overall survival. The results show that overall postoperative complication rate, anastomotic leakage, and cardiopulmonary complications are associated with a decreased long-term survival after an esophagectomy.
DISEASES OF THE ESOPHAGUS
(2023)
Article
Gastroenterology & Hepatology
J. C. H. B. M. Luijten, M. H. P. Verstegen, F. van Workum, G. A. P. Nieuwenhuijzen, M. I. van Berge Henegouwen, S. S. Gisbertz, B. P. L. Wijnhoven, R. H. A. Verhoeven, C. Rosman
Summary: It is unclear whether Ivor Lewis (IL) or McKeown (McK) esophagectomy is preferred in patients with potentially curable esophageal or gastro-esophageal junction (GEJ) cancer. A study in the Netherlands found similar survival rates and complication rates between patients who underwent IL or McK esophagectomy. Therefore, both IL and McK esophagectomy can be considered for patients with mid to distal esophageal and GEJ cancer.
DISEASES OF THE ESOPHAGUS
(2023)
Article
Gastroenterology & Hepatology
Sander Ubels, Bastiaan Klarenbeek, Moniek Verstegen, Stefan Bouwense, Ewen A. Griffiths, Frans van Workum, Camiel Rosman, Gerjon Hannink
Summary: This study aimed to develop a prediction model for postoperative mortality in patients with Anastomotic leak (AL) after esophagectomy. The developed model combines patient-related and leak-related factors to accurately predict postoperative mortality and can be useful for clinicians during counselling of patients and their families.
DISEASES OF THE ESOPHAGUS
(2023)
Article
Oncology
Sander Ubels, Eric Matthee, Moniek Verstegen, Bastiaan Klarenbeek, Stefan Bouwense, Mark I. van Berge Henegouwen, Freek Daams, Jan Willem T. Dekker, Marc J. van Det, Stijn van Esser, Ewen A. Griffiths, Jan Willem Haveman, Grard Nieuwenhuijzen, Peter D. Siersema, Bas Wijnhoven, Gerjon Hannink, Frans van Workum, Camiel Rosman, Joos Heisterkamp, Fatih Polat, Jeroen Schouten, Pritam Singh
Summary: This study aimed to assess the differences in failure to rescue (FTR) after anastomotic leak (AL) between different centers. The results showed that high-volume centers had lower FTR, which could be explained by lower leak severity, lower secondary ICU readmission rate, and higher availability of therapeutic modalities.
Article
Nanoscience & Nanotechnology
Nils L. Liebe, Ingo Mey, Loan Vuong, Fadi Shikho, Burkhard Geil, Andreas Janshoff, Claudia Steinem
Summary: The creation of artificial lipid bilayers on planar supports allows for a controlled study of membrane-confined processes. In this study, actomyosin networks were bound to supported planar lipid bilayers doped with phosphatidylinositol-4,5-bisphosphate (PtdIns[4,5]P2) via the membrane-actin linker ezrin. By analyzing this membrane system using high-resolution fluorescence microscopy, the researchers were able to understand the connectivity and contractility of the actomyosin network. They found that the network's architecture and dynamics depend not only on the PtdIns[4,5]P2 concentration, but also on the presence of negatively charged phosphatidylserine (PS).
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Silan Toy, Joern Dietz, Peter Naumann, Janina Trothe, Franziska Thomas, Ulf Diederichsen, Claudia Steinem, Andreas Janshoff
Summary: Cell adhesion molecules, such as E-cadherin, play a crucial role in various biological processes. Researchers synthesized E-cadherin mimetic peptides and attached them to solid-supported membranes on colloidal probes to identify this molecule on cell surfaces. Two different functionalization strategies were established, and binding studies showed successful attachment of the peptides to the membrane surface. The presence of E-cadherin on living cells was confirmed using colloidal probes functionalized with HAV-peptides.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Gastroenterology & Hepatology
Jobbe Lemmens, Bastiaan Klarenbeek, Moniek Verstegen, Frans van Workum, Gerjon Hannink, Sander Ubels, Camiel Rosman
Summary: This study aimed to assess the performance of a consensus-based algorithm for diagnosing anastomotic leak after minimally invasive esophagectomy. The algorithm identified patients suspected of anastomotic leak based on clinical signs and laboratory tests, and further diagnostic tests were used to confirm the leak. The algorithm showed poor performance with low sensitivity on initial assessment, indicating the need for repeated assessment to confirm leaks.
DISEASES OF THE ESOPHAGUS
(2023)
Article
Oncology
Tiuri E. Kroese, Hanneke W. M. van Laarhoven, Sebastian F. Schoppman, Pieter R. A. J. Deseyne, Eric van Cutsem., Karin Haustermans., Philippe. Nafteux, Melissa Thomas, Radka Obermannova, Hanna R. Mortensen, Marianne Nordsmark, Per Pfeiffer, Anneli Elme, Antoine Adenis, Guillaume Piessen, Christiane J. Bruns, Florian Lordick, Ines Gockel, Markus Moehler, Cihan Gani, Theodore Liakakos, John Reynolds, Alessio G. Morganti, Riccardo Rosati, Carlo Castoro, Francesco Cellini, Domenico D'Ugo, Franco Roviello, Maria Bencivenga, Giovanni de Manzoni, Mark I. van Berge Henegouwen, Maarten C. C. M. Hulshof, Jolanda van Dieren, Marieke Vollebergh, Johanna W. van Sandick, Paul Jeene, Christel T. Muijs, Marije Slingerland, Francine E. M. Voncken, Henk Hartgrink, Geert-Jan Creemers, Maurice J. C. van der Sangen, Grard Nieuwenhuijzen, Maaike Berbee, Marcel Verheij, Bas Wijnhoven, Laurens V. Beerepoot, Nadia H. Mohammad, Stella Mook, Jelle P. Ruurda, Piotr Kolodziejczyk, Wojciech P. Polkowski, Lucjan Wyrwicz, Maria Alsina, Manuel Pera, Tania F. Kanonnikoff, Andres Cervantes, Magnus Nilsson, Stefan Monig, Anna D. Wagner, Matthias Guckenberger, Ewen A. Griffiths, Elizabeth Smyth, George B. Hanna, Sheraz Markar, M. Asif Chaudry, Maria A. Hawkins, Edward Cheong, Richard van Hillegersberg, Peter S. N. van Rossum
Summary: This study aimed to develop a multidisciplinary European consensus statement on the definition, diagnosis and treatment of oligometastatic oesophago-gastric cancer. Through a series of discussions and expert evaluations, a consensus was reached, which can be used for future research in clinical trials.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Pathology
Maria J. Valkema, Anne-Marie Vos, Rachel S. van der Post, Ariadne H. A. G. Ooms, Lindsey Oudijk, Ben M. Eyck, Sjoerd M. Lagarde, Bas P. L. Wijnhoven, Bastiaan R. Klarenbeek, Camiel Rosman, J. Jan B. van Lanschot, Michail Doukas
Summary: Oesophageal adenocarcinomas may exhibit different histopathological patterns, such as excessive acellular mucin pools, signet-ring cells, and poorly cohesive cells. These components have been suggested to correlate with poor outcomes after neoadjuvant chemoradiotherapy. However, their independent effects and potential confounders have not been thoroughly studied. In this study, we evaluated the presence of extracellular mucin, signet-ring cells, and poorly cohesive cells before and after treatment, and their association with pathological response and prognosis. The findings suggest that the presence of these factors is not related to pathological response, but a pre-treatment presence of ≥10% poorly cohesive cells and a post-treatment presence of ≥1% signet-ring cells may indicate inferior prognosis.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2023)
Correction
Surgery
Bastiaan R. Klarenbeek, Hitoshi Fujiwara, Mirre Scholte, Maroeska Rovers, Atsushi Shiozaki, Camiel Rosman
BRITISH JOURNAL OF SURGERY
(2023)
Article
Oncology
Danielle J. M. Adriaans, Fanny B. M. Heesakkers, Joep A. W. Teijink, Angelique T. M. Dierick-van Daele, Jan Willem Haveman, Meindert N. Sosef, Jan Willem van den Berg, Marc J. van Det, Henk H. Hartgrink, Walther J. B. M. Jansen, Camiel Rosman, Sjoerd M. Lagarde, Stijn van Esser, Erwin van der Harst, Hanneke W. M. van Laarhoven, Grard A. P. Nieuwenhuijzen
Summary: This study aimed to gain consensus on healthcare professionals' perspectives on the self-management support information needs of esophageal cancer patients during the preoperative phase. A survey was created based on literature, clinical consultations, and patient information leaflets. A total of 33 important topics were identified, and consensus was reached on the preferred sources of support, which were face-to-face contact and a case manager.
EUROPEAN JOURNAL OF CANCER CARE
(2023)