期刊
BEILSTEIN JOURNAL OF NANOTECHNOLOGY
卷 1, 期 -, 页码 135-141出版社
BEILSTEIN-INSTITUT
DOI: 10.3762/bjnano.1.16
关键词
dye-linked L-proline dehydrogenase; electrocatalysis; electron transfer; multi-walled carbon nanotube
资金
- Scientific Research Foundation for Returned Overseas Chinese Scholars (SRF for ROCS), Ministry of Education, P.R. China
- Natural Science Foundation of Tianjin, Tianjin City, P.R. China [06YFJMJC15000]
- Tianjin Polytechnic University
- Japan Student Service Organization (JASSO)
A glassy carbon electrode (GC) was modified by multi-walled carbon nanotubes (MWCNTs). The modified electrode showed a pair of redox peaks that resulted from the oxygen-containing functional groups on the nanotube surface. A recombinant thermostable dye-linked L-proline dehydrogenase (L-proDH) from hyperthermophilic archaeon (Thermococcus profundus) was further immobilized by physical adsorption. The modified electrode (GC/MWCNTs/L-proDH) exhibited an electrocatalytic signal for L-proline compared to bare GC, GC/L-proDH and GC/MWCNTs electrodes, which suggested that the presence of MWCNTs efficiently enhances electron transfer between the active site of enzyme and electrode surface. The immobilized L-proDH showed a typical Michaelis-Menten catalytic response with lower apparent constant.
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