Article
Chemistry, Multidisciplinary
Yueming Wu, Yaofa Lin, Zihao Cong, Kang Chen, Ximian Xiao, Xue Wu, Longqiang Liu, Yunrui She, Shiqi Liu, Ruiyi Zhou, Gang Yin, Xiaoyan Shao, Yidong Dai, Haodong Lin, Runhui Liu
Summary: The PMMA@polymer, designed as a synthetic mimic of host defense peptide, shows potent activity against MRSA, superior therapeutic efficacy in vivo, and low toxicity, suggesting great potential as an effective treatment for MRSA-infected chronic osteomyelitis.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Food Science & Technology
Anai Leon Madrazo, Maira Rubi Segura Campos
Summary: This study evaluated the potential of chia-derived peptides as alternative preservatives in food. The peptides showed antibacterial activity and could prevent biofilm formation. One peptide (KKLLKI) exhibited promising antibacterial effects against Salmonella spp. on refrigerated pork meat samples.
JOURNAL OF FOOD SCIENCE
(2023)
Article
Infectious Diseases
Emanuela Roscetto, Rosa Bellavita, Rossella Paolillo, Francesco Merlino, Nicola Molfetta, Paolo Grieco, Elisabetta Buommino, Maria Rosaria Catania
Summary: This study confirmed the antimicrobial activity of two analogues of Temporin L against carbapenemase-producing K. pneumoniae, with peptide C showing stronger antimicrobial properties and bactericidal activity. The results highlight the great potential of antimicrobial peptides as a new treatment option for difficult-to-treat infections and as a new strategy for drug-resistance control.
Article
Biophysics
Barbara Claro, Antonio Peon, Eva Gonzalez-Freire, Erik Goormaghtigh, Manuel Amorin, Juan R. Granja, Rebeca Garcia-Fandino, Margarida Bastos
Summary: Antimicrobial D,L-alpha-cyclic peptides have shown to form complex macromolecular assemblies in the presence of membranes, depending on the negatively charged content of the membrane and lipid/peptide ratio. Experimental techniques and simulations suggest that CPs can self-assemble at the lipid surface into nanotubes or micellar aggregates, revealing possible mechanisms of action involving membrane segregation or disintegration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Biochemistry & Molecular Biology
Linlin Kang, Tingting Han, Hailin Cong, Bing Yu, Youqing Shen
Summary: With the rapid development of molecular biology and biochemical technology, significant progress has been made in the study of peptides. Peptides have various functions and applications, making the efficient preparation and application of bioactive peptides a research focus.
Article
Microbiology
Zhenheng Lai, Hongyu Chen, Xiaojie Yuan, Jiahui Tian, Na Dong, Xingjun Feng, Anshan Shan
Summary: The rapid development of antimicrobial resistance and slow progress in developing new antibiotics have led to a serious threat of multidrug-resistant bacterial infections. Antimicrobial peptides (AMPs) offer a promising alternative, but further research is needed to address their limitations. In this study, we designed a series of novel double-site lipidated peptide amphiphiles with a broad spectrum of antimicrobial activities. One specific peptide, WL-C-6, exhibited strong inhibition of multidrug-resistant bacteria without significant toxicity towards mammalian cells. Furthermore, WL-C-6 maintained its antibacterial properties even at physiological salt ion concentrations and remained intact after exposure to different proteases. The study also revealed that WL-C-6 rapidly disrupts bacterial membranes, leading to bacterial death. Overall, WL-C-6 shows great promise as a substitute for conventional antibiotics in combating the growing threat of multidrug-resistant bacterial infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tania Vanzolini, Michela Bruschi, Andrea C. Rinaldi, Mauro Magnani, Alessandra Fraternale
Summary: This review focuses on synthetic peptides and their multifaceted mechanisms of action as antiviral, antibacterial, and antifungal agents. Synthetic peptides have been shown to affect pathogen structures, disrupt microbial communities, and influence viral life cycles, making them promising tools against emerging infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Kan Jiang, Ximing Chen, Wei Zhang, Yehong Guo, Guangxiu Liu
Summary: In this study, a strain of Streptomyces agglomeratus 5-1-3 with strong resistance to MRSA was isolated from soil in the Qinghai-Tibet Plateau. Three NRP compounds with antibacterial activity were isolated from this strain, and echinomycin showed excellent anti-MRSA activity with a MIC of 2.0 μg/mL.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Victor H. O. Munhoz, Carolina S. Ferreira, Lucio O. Nunes, Talita L. Santos, Christopher Aisenbrey, Regina Adao, Antonio Flavio de Carvalho Alcantara, Mariana T. Q. de Magalhaes, Dorila Pilo-Veloso, Jarbas M. Resende, Burkhard Bechinger, Rodrigo M. Verly
Summary: This study found that epimers of peptides containing D-amino acids may exhibit more effective antimicrobial activity compared to their L-amino acid epimers, with D-epimers showing higher activity against bacteria like Staphylococcus aureus and Xanthomonas coconopodis. This is attributed to specific aromatic interactions involving the phenylalanine side chains of D-epimers, which enhance amphipathicity and anchoring in the hydrophobic core of the phospholipid bilayer, indicating a crucial role of the N-terminus in enhancing interaction with the membrane interface.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Plant Sciences
Yana Ilieva, Georgi Momekov, Maya Margaritova Zaharieva, Teodor Marinov, Zlatina Kokanova-Nedialkova, Hristo Najdenski, Paraskev T. Nedialkov
Summary: Two new bicyclo[3.3.1]nonane type bicyclic polyprenylated acylphloroglucinol derivatives (BPAPs), olympiforin A and B, as well as three known prenylated phloroglucinols, were isolated from Hypericum olympicum L. and their structures were determined by spectral techniques. The compounds showed significant in vitro cytotoxicity against human tumor cell lines and selectivity towards tumor cells. They also exhibited antibacterial and antibiofilm activity, particularly against Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA). ADME and drug likeness tests were performed on the compounds.
Article
Biotechnology & Applied Microbiology
Zhou Jiale, Jiao Jian, Tan Xinyi, Xie Haoji, Huang Xueqin, Wang Xiao
Summary: A mutant peptide 1018M was designed in this study with enhanced antibacterial and biofilm activity against MRSA. 1018M showed better stability and higher antimicrobial efficacy compared to the parental peptide IDR-1018.
Article
Chemistry, Multidisciplinary
Paolo Dognini, Talhat Chaudhry, Giulia Scagnetti, Michele Assante, George S. M. Hanson, Kehinde Ross, Francesca Giuntini, Christopher R. Coxon
Summary: This study presents a method for peptide cyclisation using polyfluorinated aromatic reagents and thiolates through nucleophilic aromatic substitution (SNAr). The resulting peptide scaffolds offer new possibilities for 3D peptide architectures. The method demonstrates chemoselectivity and wide applicability, allowing for stapling and multicyclisation of non-protected peptides. Additionally, a porphyrin-templated stapled peptide with skin cell penetrating ability and intrinsic fluorescence was developed.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Medicinal
Sen Li, Meng Wang, Shengcong Chen, Maxwell Ampomah-Wireko, Chen Gao, Ziwei Xia, Lauraine Nininahazwe, Shangshang Qin, En Zhang
Summary: The development of bacterial resistance to conventional antibiotics has posed challenges in treating bacterial infections. In this study, a series of cationic compounds with different linkers were designed and synthesized, showing high antibacterial activity against Gram-negative and Gram-positive bacteria. The best compound, 3e, demonstrated excellent antibacterial activity and low hemolytic toxicity. Mechanism studies revealed its potential bactericidal mechanism and its ability to effectively reduce the load of methicillin-resistant Staphylococcus aureus (MRSA). These findings highlight the significance of divergent linkers in antimicrobial peptide mimetics for treating bacterial infections.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Qi An Ang, Giovinna Arfan, Chu Yang Fann Ong, Fui Mee Ng, Esther H. Q. Ong, Cheng San Brian Chia
Summary: Staphylococcus aureus, especially methicillin-resistant MRSA, is a major cause of human skin infections. A novel cationic nonapeptide, nonapeptide 11, has been designed with potent anti-MRSA activity and rapid bactericidal action, showing potential as a mupirocin replacement candidate for treating skin infections caused by MRSA.
CHEMICAL BIOLOGY & DRUG DESIGN
(2021)
Article
Chemistry, Organic
Dessy Yulyani Kurnia, Rani Maharani, Ace Tatang Hidayat, Jamaludin Al-Anshori, Ika Wiani, Tri Mayanti, Nurlelasari, Desi Harneti, Unang Supratman
Summary: Xylapeptide B, derived from the fungus of the Chinese medicinal plant Sophora tonkinensis, was successfully synthesized using a combination of solid- and solution-phase methods. The synthesis involved the use of HBTU/HOBt coupling reagents and D-Ala as the C-terminal.
JOURNAL OF HETEROCYCLIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Dianna Truong, Nelson Y. S. Lam, Meder Kamalov, Mie Riisom, Stephen M. F. Jamieson, Paul W. R. Harris, Margaret A. Brimble, Nils Metzler-Nolte, Christian G. Hartinger
Summary: The study presents a novel approach for selectively anchoring transition metal half-sandwich complexes onto peptides with high purity using solid support synthesis. The metalated peptides were evaluated for their anticancer properties against human cancer cell lines, showing no cytotoxic activity but potential for site-selective peptide labelling and ligand design in transition metal catalysts.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Daniel Obitz, Karmel S. Gkika, Marvin Heller, Tia E. Keyes, Nils Metzler-Nolte
Summary: A lanthanide(iii) complex connected to a light harvesting antenna was synthesized and tested as a potential photosensitizer in photodynamic therapy. The complex showed significant phototoxic activity on cancer cells upon irradiation, with ROS production identified as the compound's phototoxic mode of action. In cell viability assays, a 10-fold decrease in IC50 value was observed compared to the dark control.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Marion Graf, Jasmine Ochs, Nils Metzler-Nolte, Peter Mayer, Hans-Christian Bottcher
Summary: This paper describes the synthesis and characterization of six new organometallic half-sandwich iridium(III) compounds with modified bipyridine ligands. The cytotoxic properties of the compounds against two cancer cell lines were evaluated, and compound 3 showed the most remarkable biological activity with significantly enhanced toxicity compared to cisplatin.
ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
(2023)
Article
Chemistry, Multidisciplinary
Paramita Sarkar, Kathakali De, Malvika Modi, Geetika Dhanda, Richa Priyadarshini, Julia E. Bandow, Jayanta Haldar
Summary: Resistance to vancomycin requires developing alternative therapeutics. In this study, vancomycin derivatives with mechanisms beyond d-Ala-d-Ala binding were reported. The lead molecule, VanQAmC(10), inhibits bacterial cell division, which is a previously unknown property for glycopeptide antibiotics.
Article
Biochemical Research Methods
Boudewijn Hollebrands, Jos A. Hageman, Jasper W. van de Sande, Bauke Albada, Hans-Gerd Janssen
Summary: Peptides play a significant role in determining the taste of food products, with their taste impressions depending on the amino acids present and their sequence. Identifying short peptides in foods is challenging, but a method was developed to accurately predict retention times of short peptides using reversed phase separation. By training a model using a combination of peptide descriptors and a newly derived set of amino acid index derived sequence-specific peptide descriptors, the model achieved a high accuracy in predicting retention times and elution order for 93% of the short peptides.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Bauke Albada
Summary: Bioconjugation of chemical entities to biologically active proteins has provided valuable insights into cellular processes and yielded innovative therapeutic agents. However, a current challenge lies in efficiently generating uniform conjugates of native proteins in their natural environment. By combining various features of protein-modifying enzymes, artificial constructs have been developed. This review evaluates the current status of this approach, with a focus on the protein-binding anchor, modification chemistry, linker, and suggests the incorporation of trigger-responsive switches for regulating protein modification.
Article
Chemistry, Multidisciplinary
Johannes A. M. Damen, Jorge Escorihuela, Han Zuilhof, Floris L. van Delft, Bauke Albada
Summary: The rates of reaction between strained cycloalkynes and cycloalkenes with 1,2-quinone were studied using stopped flow UV-Vis spectroscopy and computational analysis. The results showed that strained alkyne BCN OH 3 reacted over 150 times faster than strained alkene TCO-OH 5, and the introduction of a carbamate derivative reduced the rate constant by almost half. Additionally, the 8-membered strained alkyne BCN OH 3 reacted 16 times faster than the more strained 7-membered THS 2. Thermodynamic activation parameters were determined using the linearized Eyring equation, providing valuable insights into these rapid click reactions.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Medicinal
Irina Kollmorgen, Nathalie Bachmann, Michael Dal Molin, Carsten Degenhart, Eldar Zent, Vikram Pareek, Uwe Koch, Jan Rybniker, Nils Metzler-Nolte, Raphael Stoll, Bert Klebl, Julia Elisabeth Bandow, Jurgen Scherkenbeck
Summary: Due to increasing resistances, there is a need for antibacterial compounds with unique modes of action. One such compound, moiramide B, shows strong activity against gram-positive bacteria but weaker activity against gram-negative bacteria. The structure-activity relationship of moiramide B presents a challenge for optimization strategies, while the fatty acid tail is considered as a transport vehicle. However, the sorbic acid unit is found to be relevant for ACC inhibition and allows the development of moiramide derivatives with altered antibacterial profiles.
Article
Chemistry, Organic
Jasper W. van de Sande, Bauke Albada
Summary: The researchers reported the total synthesis of post-translationally modified peptides, including beta-1,2-linked L-arabinosylated Fmoc-protected hydroxyproline building blocks and sulfated tyrosine and hydroxyproline, which are important regulating molecules for living organisms. Clean glycopeptides were obtained by removing acetyl groups from the L-arabinose and neopentyl-protected sulfated tyrosine before deprotection.
Article
Biochemical Research Methods
Melissa Vazquez-Hernandez, Stephanie L. Leedom, Kenneth C. Keiler, Julia Elisabeth Bandow
Summary: Trans-translation is the most effective ribosome rescue system in bacteria, but Bacillus subtilis possesses two alternative ribosome rescue mechanisms. In this study, the proteomes of B. subtilis 168 and a ssrA mutant were compared using quantitative proteomics. The mutant strain showed lower growth rate and protein synthesis rates, altered motility and chemotaxis phenotype, and alterations in the abundance of ribosomal proteins and proteins related to amino acid biosynthesis, reflecting slow ribosome recycling.
Article
Infectious Diseases
Leonie Sagurna, Sascha Heinrich, Lara-Sophie Kaufmann, Christian Rueckert-Reed, Tobias Busche, Alexander Wolf, Jan Eickhoff, Bert Klebl, Joern Kalinowski, Julia E. Bandow
Summary: Bacteria of the genus Streptomyces produce various specialized metabolites. Single biosynthetic gene clusters (BGCs) can give rise to different products with varying biological activities. In this study, alnumycin and 1,6-dihydro 8-propylanthraquinone were isolated from a Streptomyces strain containing the alnumycin BGC. Both compounds exhibited antimicrobial activity, with 1,6-dihydro 8-propylanthraquinone showing higher activity against E. coli & UDelta;tolC compared to alnumycin. The modes of action of these compounds are worth further investigation.
Review
Biochemistry & Molecular Biology
Jaccoline Zegers, Maartje Peters, Bauke Albada
Summary: This article summarizes the importance of stabilizing guanine quadruplexes (G4s) for cancer treatment and explores the methods and techniques for stabilizing G4s using metal complexes. By analyzing the G4-stabilizing capacity and bioactivity data of 74 metal-based ligands, guidelines for future studies in this field are proposed.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Tim Dirks, Marco Krewing, Katharina Vogel, Julia E. Bandow
Summary: Cold atmospheric pressure plasmas have a significant impact on protein aggregation, and Hsp33 plays an important role in preventing protein aggregation. It has been found that over-production of Hsp33 can increase bacterial resistance to cold plasmas.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Multidisciplinary Sciences
Tim Dirks, Abdulkadir Yayci, Sabrina Klopsch, Marco Krewing, Wuyuan Zhang, Frank Hollmann, Julia E. Bandow
Summary: This study demonstrates that immobilization universally protects enzymes under plasma-operating conditions and improves their activity, opening up new possibilities for emerging applications.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Correction
Chemistry, Multidisciplinary
Christine N. Morrison, Kathleen E. Prosser, Ryjul W. Stokes, Anna Cordes, Nils Metzler-Nolte, Seth M. Cohen
Summary: The study introduces a novel approach using metallofragments as 3D scaffolds for fragment-based drug discovery. This method has great potential in the field of medicinal chemistry and provides a new direction for drug development.