Article
Multidisciplinary Sciences
Therese Woodring, Colin N. Dewey, Lucas Dos Santos Dias, Xin He, Hannah E. Dobson, Marcel Wuthrich, Bruce Klein
Summary: Using single cell transcriptome analysis, we identified transcriptionally diverse and distinctive antigen-specific T cells associated with protective vaccine-induced immunity. These cells exhibited a strong type I interferon response and high expression of the natural killer cell marker gene NKG7. We also observed differences in TCR gene use and Th1 and Th17 skewed responses after protective and nonprotective vaccination.
Review
Immunology
Vincent Dussupt, Kayvon Modjarrad, Shelly J. Krebs
Summary: The unprecedented Zika outbreak in the Americas from 2015-2016 led to global concern and accelerated the deployment of vaccine and therapeutic countermeasures. Several potent neutralizing antibodies against Zika and related flaviviruses have been discovered in recent years. Structure-based approaches have helped identify species-specific and cross-protective epitopes with therapeutic value.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Elisa Fanunza, Fabrizio Carletti, Marina Quartu, Nicole Grandi, Laura Ermellino, Jessica Milia, Angela Corona, Maria Rosaria Capobianchi, Giuseppe Ippolito, Enzo Tramontano
Summary: The viral protein NS2A suppresses the IFN signaling and promotes degradation of STAT1 and STAT2 to evade the innate immune system, helping Zika Virus escape host immune responses. Antagonism against IFN plays a crucial role in flavivirus virulence, and understanding how Zika Virus evades IFN response can pave the way for new strategies to attenuate the pathogenesis.
Article
Cell Biology
Taylor Schmit, Kai Guo, Jitendra Kumar Tripathi, Zhihan Wang, Brett McGregor, Mitch Klomp, Ganesh Ambigapathy, Ramkumar Mathur, Junguk Hur, Michael Pichichero, Jay Kolls, M. Nadeem Khan
Summary: This study identifies IFN-gamma-regulated CCR2(+) monocytes as the main driver of lung damage during IAV infection. IFN-gamma regulates the recruitment and inflammatory phenotype of CCR2(+) monocytes. CD8+ T cells are the main source of IFN-gamma in the infected lungs.
Article
Multidisciplinary Sciences
Xinrui Zhao, Songling Hu, Liang Zeng, Xinglong Liu, Yimeng Song, Yuhong Zhang, Qianping Chen, Yang Bai, Jianghong Zhang, Haowen Zhang, Yan Pan, Chunlin Shao
Summary: This study found that radiotherapy can activate CD8(+) T cells and enhance the antitumor abscopal effect in PD-L1-deficient mouse hosts. Autophagy inhibitors can increase the release of cytokines and further activate CD8(+) T cells, promoting the efficacy of anti-tumor treatment.
Article
Biochemistry & Molecular Biology
Alessia Furgiuele, Franca Marino, Emanuela Rasini, Massimiliano Legnaro, Alessandra Luini, Maria Giulia Albizzati, Alessia di Flora, Barbara Pacchetti, Marco Cosentino
Summary: This study investigated the effects of CBD on human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells, and found that CBD can affect cytokine production, cell proliferation, and the functional properties of regulatory T cells. However, the effects of CBD on cell proliferation and protein production were inconsistent, suggesting a complex activity that warrants further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Neurosciences
Lisa Johann, Ari Waisman
Summary: The study reveals a novel mechanism by which astrocytes maintain an anti-inflammatory state in the CNS. This mechanism involves IFNg-induced TRAIL expression on astrocytes, leading to effector T cell apoptosis and suppression of inflammation.
Article
Multidisciplinary Sciences
Louise Malle, Roosheel S. Patel, Marta Martin-Fernandez, O'Jay Stewart, Quentin Philippot, Sofija Buta, Ashley Richardson, Vanessa Barcessat, Justin Taft, Paul Bastard, Julie Samuels, Clotilde Mircher, Anne-Sophie Rebillat, Louise Maillebouis, Marie Vilaire-Meunier, Kevin Tuballes, Brad R. Rosenberg, Rebecca Trachtman, Jean-Laurent Casanova, Luigi D. Notarangelo, Sacha Gnjatic, Douglas Bush, Dusan Bogunovic
Summary: Individuals with Down's syndrome (DS) exhibit multiple impairments such as cardiac, neurocognitive, and growth issues. They are also prone to severe infections and autoimmune disorders. This study reveals an autoimmune-prone state in DS characterized by persistent elevation of cytokines, chronic activation of CD4 T cells, and ongoing B cell activation. Furthermore, auto-antibodies targeting various organs and systems were detected in DS individuals' plasma, suggesting a breach in immune tolerance.
Article
Immunology
Dalila Mele, Anna Calastri, Eugenia Maiorano, Antonella Cerino, Michele Sachs, Barbara Oliviero, Stefania Mantovani, Fausto Baldanti, Raffaele Bruno, Marco Benazzo, Alba Grifoni, Alessandro Sette, Mario U. Mondelli, Stefania Varchetta
Summary: Patients with SARS-CoV-2-induced OTD develop highly functional virus-specific CD4(+) and CD8(+) T cells during the symptomatic phase of the disease, suggesting that robust and coordinated T-cell responses provide protection against extension of COVID-19 to the lower respiratory tract.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Arianna Draghi, Mario Presti, Agnete W. P. Jensen, Christopher A. Chamberlain, Benedetta Albieri, Anne-Christine K. Rasmussen, Mads H. Andersen, Michael D. Crowther, Inge Marie Svane, Marco Donia
Summary: Our study demonstrates that exploiting tumor-specific cytotoxic CD4(+) TILs could help overcome resistance to ICB mediated by IFN gamma-signaling loss in MHCIIconst(+) melanomas.
CLINICAL CANCER RESEARCH
(2023)
Article
Immunology
Amelia K. Pinto, Mariah Hassert, Xiaobing Han, Douglas Barker, Trevor Carnelley, Emilie Branche, Tara L. Steffen, E. Taylor Stone, Elizabeth Geerling, Karla M. Viramontes, Cory Nykiforuk, Derek Toth, Sujan Shresta, Shantha Kodihalli, James D. Brien
Summary: This study investigates how polyclonal antibodies interact with Zika and dengue viruses in a mouse model, using intravenous hyperimmunoglobulin (IVIG) preparations. The research shows the unique dynamics of polyclonal antibodies in providing both enhancement and protection against flavivirus infection, with implications for antibody therapeutics and vaccine development.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Noemia S. Lima, Damee Moon, Samuel Darko, Rafael A. De La Barrera, Leyi Lin, Michael A. Koren, Richard G. Jarman, Kenneth H. Eckels, Stephen J. Thomas, Nelson L. Michael, Kayvon Modjarrad, Daniel C. Douek, Lydie Trautmann
Summary: The study found that prior immunization with JEV has a positive impact on CD4 T cell responses to the ZPIV vaccine, generating more durable responses primarily directed towards conserved epitopes. T cell receptor repertoire analysis revealed preferential expansion of cross-reactive clonotypes between JEV and ZIKV.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Bram W. van Os, Winnie G. Vos, Laura A. Bosmans, Claudia M. van Tiel, Myrthe den Toom, Linda Beckers, Merel Admiraal, Marten A. Hoeksema, Menno P. de Winther, Esther Lutgens
Summary: Inhibition of CD40L has shown beneficial effects in autoimmune disease and inflammation. CD40L deficiency in T cells reduces CD4(+) T-cell activation and specifically decreases IFN-gamma-producing Th1 cells. CD40L in T cells affects cell death and proliferation. The study also identifies the mediator of CD40L reverse signaling and highlights the importance of both CD40L forward and reverse signaling.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Fisheries
Belen Alvarez, Concepcion Revilla, Sara Moreno, Angeles Jimenez-Marin, Elena Ramos, Paloma Martinez de la Riva, Teresa Poderoso, Juan J. Garrido, Angel Ezquerra, Javier Dominguez
Summary: This report describes a new monoclonal antibody, 4H5CR4, against porcine CD9, which together with other antibodies can distinguish at least five CD4(+) T cell subsets. The analysis of CD45RA, CD27, CD29, CD95, CCR7, and SLA-DR markers on these subsets reveals a progressive model of CD4(+) T cell development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Choong-Hyun Koh, Suyoung Lee, Minkyeong Kwak, Byung-Seok Kim, Yeonseok Chung
Summary: CD8 T cells play crucial roles in immune defense, and emerging evidence suggests the existence of distinct subsets with unique functions and therapeutic potential. Understanding these subsets is important for understanding immune responses in different diseases, and developing immunotherapeutic approaches targeting specific CD8 T-cell subsets holds great promise.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Chemistry, Medicinal
Sharton V. A. Coelho, Naiara M. Rust, Lucas Vellasco, Michelle P. Papa, Aline S. G. Pereira, Matheus Ferreira da Silva Palazzo, Maria Aparecida Juliano, Simone M. Costa, Ada M. B. Alves, Marli T. Cordeiro, Ernesto T. A. Marques, Julio Scharfstein, Luciana B. de Arruda
Summary: The study suggests that activation of the contact/kallikrein-kinin system (KKS) may impair endothelial function in dengue virus infection, leading to enhanced DENV replication through BK-induced apoptosis reversal via B2R. Targeting B2R can reduce viral load in brain tissues in the mouse model of cerebral dengue infection.
Article
Clinical Neurology
Luiz Ricardo C. Vasconcellos, Leticia Martimiano, Danillo Pereira Dantas, Filipe Mota Fonseca, Hilton Mata-Santos, Leonardo Travassos, Rosalia Mendez-Otero, Marcelo Torres Bozza, Pedro Moreno Pimentel-Coelho
Summary: The study established a model in mice for intrastriatal heme injection, causing lipid peroxidation, neuroinflammation, and sensorimotor deficits. Heme induced brain damage and reactive astrogliosis, along with behavior deficits. NLRP3 was found to play a role in heme-induced behavior deficits in this model.
Article
Biochemistry & Molecular Biology
Carla Andreia Abreu, Leandro Coelho Teixeira-Pinheiro, Rafael Lani-Louzada, Almir Jordao Silva-Junior, Juliana F. Vasques, Fernanda Gubert, Gabriel Nascimento-dos-Santos, Ronaldo Mohana-Borges, Eduardo de Souza Matos, Pedro Moreno Pimentel-Coelho, Marcelo Felippe Santiago, Rosalia Mendez-Otero
Summary: The study found that knockout mice lacking GD3 synthase show a deficiency in b-series gangliosides and an accumulation of a-series gangliosides in the retina, leading to reduced RGC density, light responsiveness, and visual acuity.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Cell Biology
Mariana A. Antunes, Cassia L. Braga, Taina B. Oliveira, Jamil Z. Kitoko, Ligia L. Castro, Debora G. Xisto, Mariana S. Coelho, Nazareth Rocha, Rodrigo P. Silva-Aguiar, Celso Caruso-Neves, Eduarda G. Martins, Clara Fernandes Carvalho, Antonio Galina, Daniel J. Weiss, Jose R. Lapa e Silva, Miqueias Lopes-Pacheco, Fernanda F. Cruz, Patricia R. M. Rocco
Summary: The study compared MSCs from healthy and emphysematous donors, and observed their therapeutic effects in a mouse model of severe emphysema. MSCs from emphysematous donors exhibited gene downregulation and mitochondrial abnormalities, leading to inferior therapeutic outcomes compared to MSCs from healthy donors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Renata Moll-Bernardes, Andrea Silvestre de Sousa, Ariane V. S. Macedo, Renato D. Lopes, Narendra Vera, Luciana C. R. Maia, Andre Feldman, Guilherme D. A. S. Arruda, Mauro J. C. Castro, Pedro M. Pimentel-Coelho, Denilson C. de Albuquerque, Thiago Ceccatto de Paula, Thyago A. B. Furquim, Vitor A. Loures, Karla G. D. Giusti, Nathalia M. de Oliveira, Fabio A. De Luca, Marisol D. M. Kotsugai, Rafael A. M. Domiciano, Mayara Fraga Santos, Olga Ferreira de Souza, Fernando A. Bozza, Ronir Raggio Luiz, Emiliano Medei
Summary: The study identified that interleukin-10 and interleukin-12 (p70) levels, in combination with clinical variables at hospital admission, are key biomarkers associated with an increased risk of disease progression in hypertensive patients with COVID-19.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
C. T. Azevedo, A. C. Cotias, A. C. S. Arantes, T. P. T. Ferreira, M. A. Martins, P. C. Olsen
Summary: Regulatory T cells (Tregs) play important roles in regulating responses to innocuous antigens and controlling Th2 response, and the frequencies of Treg cell subtypes are different among isogenic mouse strains with susceptibility to experimental asthma. A/J mice have higher frequency of CD4(+)IL-10(+) T cells, while C57BL/6 mice have higher frequency of CD4(+)CD25(+)Foxp3(+) Treg cells in response to allergen challenge.
MEDIATORS OF INFLAMMATION
(2021)
Article
Cell Biology
Luiz Ricardo da Costa Vasconcellos, Pedro Moreno Pimentel-Coelho
NEURAL REGENERATION RESEARCH
(2022)
Article
Clinical Neurology
Daiane Aparecida Spiess, Raquel Maria Pereira Campos, Luciana Conde, Nadine Didwischus, Johannes Boltze, Rosalia Mendez-Otero, Pedro Moreno Pimentel-Coelho
Summary: This study investigated the therapeutic effects of AMD3100 on neonatal hypoxic-ischemic encephalopathy (HIE) and found that it did not improve structural and functional outcomes in a rat HIE model.
Article
Immunology
Hernandez Moura Silva, Jamil Zola Kitoko, Camila Pereira Queiroz, Lina Kroehling, Fanny Matheis, Katharine Lu Yang, Bernardo S. Reis, Christine Ren-Fielding, Dan R. Littman, Marcelo Torres Bozza, Daniel Mucida, Juan J. Lafaille
Summary: Perivascular macrophages play a role in tissue development and disease processes, although their key features are not yet clear. Lack of the transcription factor c-MAF in perivascular macrophages leads to different effects in various organs and has significant impacts on metabolic parameters.
SCIENCE IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Tanira Giara Mello, Paulo Henrique Rosado-de-Castro, Juliana Ferreira Vasques, Carolina Pinhao, Tayna Monteiro Santos, Renata Rodrigues de Lima, Bernd Uwe Foerster, Fernando Fernandes Paiva, Rosalia Mendez-Otero, Pedro Moreno Pimentel-Coelho
Summary: Intravenous injection of human umbilical cord-derived mesenchymal stromal cells (hUC-MSC) shows limited efficacy in the treatment of intracerebral hemorrhage (ICH), especially in severe cases. Hyperacute treatment with hUC-MSC does not significantly improve the survival rate, motor function, or residual hematoma volume in severe ICH. Further optimization of hUC-MSC-based therapies is needed.
Article
Multidisciplinary Sciences
V. R. S. Monteiro, C. B. V. Andrade, H. R. Gomes, M. W. Reginatto, G. E. Imperio, K. N. Fontes, D. A. Spiess, W. S. Rangel-Junior, V. M. O. Nascimento, C. O. S. Lima, R. P. C. Sousa, F. F. Bloise, S. G. Matthews, E. Bloise, P. M. Pimentel-Coelho, T. M. Ortiga-Carvalho
Summary: Limited information is available about the effect of mid-pregnancy viral infections on placental expression and offspring behavior. This study found that maternal exposure to a synthetic double-stranded RNA viral mimic disrupted placental cell turnover and impaired offspring behavior in adult life.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Leticia Lintomen, Luciana M. Kluppel, Jamil Z. Kitoko, Elena Montes-Cobos, Vinicius M. Vidal, Luis B. Tan, Jose Nazioberto de Farias, Heitor S. de de Souza, Priscilla C. Olsen, Marcelo T. Bozza
Summary: Macrophage migration inhibitory factor (MIF) is highly expressed in BALF and serum of asthmatic patients and contributes to asthmatic pathogenesis in mice. This study investigated the role of MIF in a more complex and relevant asthma model using house dust mite (HDM) extract. Results showed that MIF deficiency reduced airway hyperresponsiveness, eosinophil infiltration, mucus hypersecretion, and subepithelial fibrosis in HDM-challenged mice. Additionally, MIF blockade prevented the increase of inflammatory cytokines IL-4, IL-5, and IL-13 in the lungs while preserving CCL11 levels. These findings suggest that MIF may be a promising target for asthma therapy.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Integrative & Complementary Medicine
Mariana Freire Campos, Leopoldo Clemente Baratto, Vinicius Mendes Vidal, Ivana Ventura Nascimento, Brendo Araujo Gomes, Genes de Lima Martins Neto, Priscilla Christina Olsen, Rodrigo Ribeiro Tarjano Leo, Lilian Oliveira Moreira
Summary: The leaf extract of M. tomentosa Benth. showed antibacterial and anti-inflammatory properties by inhibiting bacterial cell growth, promoting biofilm formation, and inhibiting cytokine and NO production induced by LPS.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Meeting Abstract
Cell & Tissue Engineering
M. C. Lourenco Filho, M. A. Antunes, C. L. Braga, T. B. Oliveira, J. Z. Kitoko, L. L. Castro, D. G. Xisto, M. S. Coelho, N. Rocha, E. G. Martins, L. R. P. de Carvalho, A. Galina, D. J. Weiss, J. Lapa e Silva, F. F. Cruz, P. R. M. Rocco