Article
Multidisciplinary Sciences
Carmen Aguilar, Mindaugas Pauzuolis, Malvika Pompaiah, Ehsan Vafadarnejad, Panagiota Arampatzi, Mara Fischer, Dominik Narres, Mastura Neyazi, Ozge Kayisoglu, Thomas Sell, Nils Bluethgen, Markus Morkel, Armin Wiegering, Christoph-Thomas Germer, Stefan Kircher, Andreas Rosenwald, Antoine-Emmanuel Saliba, Sina Bartfeld
Summary: In this study, it was found that Helicobacter pylori prefers to attach to differentiated cells in the pit region of gastric units. Organoid models were used to validate this phenomenon, and it was discovered that differentiated pit cells with high levels of GKN1, GKN2 and PSCA were more susceptible to H. pylori attachment and CagA translocation. It was also found that attachment was unrelated to the expression of MUC5AC or PSCA, but relied on bacterial chemotaxis towards host cell-released urea, which was proportional to the size of the host cell.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Microbiology
Agustina Taglialegna
Summary: In this study, Sharafutdinov et al. report the identification of a single nucleotide polymorphism in the gene encoding the HtrA protease of Helicobacter pylori that is linked to gastric cancer.
NATURE REVIEWS MICROBIOLOGY
(2023)
Review
Health Care Sciences & Services
Vyshnavy Balendra, Chiara Amoroso, Barbara Galassi, Josephine Esposto, Claudia Bareggi, Jennie Luu, Lucia Scaramella, Michele Ghidini
Summary: High-salt diet and H. pylori infection are important factors contributing to gastric cancer development, with their combined impact increasing the risk of gastric adenocarcinoma. Mechanisms such as disruption of mucosal barriers and induction of inflammatory responses play a role in the development of gastric cancer. Therefore, reducing salt intake and using antibacterial therapy may potentially lower the susceptibility to gastric cancer.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Multidisciplinary Sciences
Tomohiko Yasuda, Hyun Seok Lee, Su Youn Nam, Hiroto Katoh, Yuko Ishibashi, Somay Yamagata Murayama, Hidenori Matsui, Hiroki Masuda, Emiko Rimbara, Nobuyuki Sakurazawa, Hideyuki Suzuki, Hiroshi Yoshida, Yasuyuki Seto, Shumpei Ishikawa, Seong Woo Jeon, Masahiko Nakamura, Sachiyo Nomura
Summary: Genetic analysis and culturing techniques for gastric non-Helicobacter pylori Helicobacter (NHPH) are advancing, with NHPH reported to accompany certain gastric conditions. A study on Korean gastric cancer patients found a low percentage positive for NHPH, suggesting it may play a role in gastric cancer development, albeit with lower pathogenicity compared to H. pylori.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Gurdeep Chahal, Medea Padra, Mattias Erhardsson, Chunsheng Jin, Macarena Quintana-Hayashi, Vignesh Venkatakrishnan, Janos Tamas Padra, Helen Stenback, Anders Thorell, Niclas G. Karlsson, Sara K. Linden
Summary: This study found that Helicobacter pylori infection in the stomach results in increased diversity and variation in glycan structures, primarily influenced by the binding ability of the BabA protein to fucosylated structures.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Medicine, General & Internal
Yoshiaki Usui, Yukari Taniyama, Mikiko Endo, Yuriko N. Koyanagi, Yumiko Kasugai, Isao Oze, Hidemi Ito, Issei Imoto, Tsutomu Tanaka, Masahiro Tajika, Yasumasa Niwa, Yusuke Iwasaki, Tomomi Aoi, Nozomi Hakozaki, Sadaaki Takata, Kunihiko Suzuki, Chikashi Terao, Masanori Hatakeyama, Makoto Hirata, Kokichi Sugano, Teruhiko Yoshida, Yoichiro Kamatani, Hidewaki Nakagawa, Koichi Matsuda, Yoshinori Murakami, Amanda B. Spurdle, Keitaro Matsuo, Yukihide Momozawa
Summary: This study reveals that certain genetic variants associated with Helicobacter pylori infection can increase the risk of gastric cancer. Individuals carrying these genetic variants and infected with H. pylori have an even higher risk of developing gastric cancer.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Medicine, Research & Experimental
Anne-Sophie Fischer, Stefanie Muellerke, Alexander Arnold, Julian Heuberger, Hilmar Berger, Manqiang Lin, Hans-Joachim Mollenkopf, Jonas Wizenty, David Horst, Frank Tacke, Michael Sigal
Summary: The stomach corpus epithelium exhibits organized cellular differentiation controlled by RSPO3 during homeostasis and in response to chronic Helicobacter pylori infection, but its absence promotes pit cell differentiation. RSPO3 plays a crucial role in the recovery of corpus glands after injury, but in the context of chronic H. pylori infection, it leads to severe glandular hyperproliferation and the development of premalignant metaplasia.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Oncology
Mahjerin Nasrin Reza, Shahin Mahmud, Nadim Ferdous, Ishtiaque Ahammad, Mohammad Uzzal Hossain, Md. Al Amin, A. K. M. Mohiuddin
Summary: In this study, two siRNA molecules were designed to silence the CagA and VacA genes of H. pylori, which are significantly involved in gastric cancer development. These designed siRNAs should effectively silence the CagA and VacA genes during siRNA mediated treatment in gastric cancer.
Article
Cell Biology
Qiuhua Deng, Yifei Xu, Yuanzun Zhong, Liyao Tang, Si Du, Jiongming Yang, Lingping Wu, Shaoju Guo, Bin Huang, Hongying Cao, Ping Huang
Summary: Persistent Helicobacter pylori infection can evade clearance by inhibiting xenophagy through miRNA regulation of key autophagy-related genes. miR-30c-1-3p and miR-30c-5p can reduce the expression of ATG14, ULK1, and ATG12, resulting in decreased intracellular elimination of H. pylori.
CELLULAR MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Silvia Salvatori, Irene Marafini, Federica Laudisi, Giovanni Monteleone, Carmine Stolfi
Summary: H. pylori infection contributes to the onset and development of gastric cancer through the mechanisms of chronic inflammation and DNA damage to gastric epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Lusheng Song, Minkyo Song, Charles S. Rabkin, Stacy Williams, Yunro Chung, Jennifer Van Duine, Linda M. Liao, Kailash Karthikeyan, Weimin Gao, Jin G. Park, Yanyang Tang, Jolanta Lissowska, Ji Qiu, Joshua LaBaer, M. Constanza Camargo
Summary: This study evaluated humoral responses to a nearly complete H. pylori immunoproteome among GC cases and controls, finding higher prevalence of certain antibodies in controls and lower seroprevalence in GC cases, suggesting immune protection and potential changes in specific proteins.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Gastroenterology & Hepatology
Emily Taillieu, Chloe De Witte, Heiko De Schepper, Wouter Van Moerkercke, Sophie Rutten, Stijn Michiels, Yuna Arnst, Sofie De Bruyckere, Sven Francque, Frauke van Aert, Christophe George, Emma Callewaert, Tiene Callewaert, Glenn Vanneste, Erik Vanderstraeten, Nina Van Heddegem, Margaux Vansteelant, Koen Chiers, Freddy Haesebrouck, Christophe Van Steenkiste
Summary: This study reveals that gastric non-Helicobacter pylori Helicobacter (NHPH) species naturally associated with animals are linked with gastric disease in human patients. The findings suggest that routine PCR testing for zoonotic gastric NHPHs should be conducted on patients with gastric complaints to diagnose potential pathogens.
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Immunology
Yvonne L. Latour, Johanna C. Sierra, Kara M. McNamara, Thaddeus M. Smith, Paula B. Luis, Claus Schneider, Alberto G. Delgado, Daniel P. Barry, Margaret M. Allaman, Wade Calcutt, Kevin L. Schey, M. Blanca Piazuelo, Alain P. Gobert, Keith T. Wilson
Summary: Colonization by Helicobacter pylori is associated with gastric diseases, and the interplay of the host response and the pathogen affects the outcome. Polyamines have been found to regulate H. pylori-induced inflammation. Specific deletion of the ODC gene in gastric epithelial cells can reduce gastritis, attenuate epithelial proliferation, and downregulate the expression of immune mediators induced by H. pylori.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Oncology
Yu Song, Dong Guo, Jia-Fei Liu, Li-Na Ge, Peng Liu, Ye-Min Qu, Hai-Yan Cong, Tian Li, Xin Chang, Yi-Ran Wang, Li-Ying Shao, Zong-Jun Du, Ming-Yi Wang
Summary: This study found that Helicobacter pylori (H. pylori) infection is closely related to the development of gastric cancer (GC), but the association between aberrant microRNA (miRNA) expression and H. pylori-induced GC is not well understood. miRNA sequencing revealed that miR-7 and miR-153 were significantly decreased in CagA positive GC tissues, and this was confirmed in a chronic infection model. Functional experiments showed that miR-7 and miR-153 can promote apoptosis and autophagy, inhibit proliferation and inflammatory response in GES-1/HP cells. Downregulation of miR-7 and miR-153 may be useful in diagnosing H. pylori (CagA+)-induced GC.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2023)
Review
Microbiology
Ling Zhang, Ming Zhao, Xiangsheng Fu
Summary: Helicobacter pylori (H. pylori) infection is a common cause of gastric disease, but the increase in antibiotic resistance has made it challenging to eradicate. The stomach has a unique microecology that can have positive, negative, or neutral effects on microbial fitness and function. This article reviews the imbalance of gastric microecology and its relationship with H. pylori, as well as potential methods for modulating gastric microecology to facilitate H. pylori eradication, such as probiotics, prebiotics, postbiotics, synbiotics, and microbiota transplantation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Kodai Abe, Yasuaki Kabe, Susumu Uchiyama, Yuka W. Iwasaki, Hirotsugu Ishizu, Yoshifumi Uwamino, Toshiki Takenouchi, Shunsuke Uno, Makoto Ishii, Takahiro Maruno, Masanori Noda, Mitsuru Murata, Naoki Hasegawa, Hideyuki Saya, Yuko Kitagawa, Koichi Fukunaga, Masayuki Amagai, Haruhiko Siomi, Makoto Suematsu, Kenjiro Kosaki
Summary: Recent clinical trial showed 3CL(pro) inhibitor significantly reduces risk of hospitalization/death, while SARS-CoV-2 sub-lineage with reduced 3CL(pro) enzymatic activity exhibits milder clinical course. Structural perturbation surrounding substrate-binding region of mutant 3CL(pro) may explain the decreased activity.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Hiromi Yamada, Kazumichi M. Nishida, Yuka W. Iwasaki, Yosuke Isota, Lumi Negishi, Mikiko C. Siomi
Summary: Siwi promotes the phosphorylation of Papi, enhancing its binding ability to Siwi-bound piRNA precursors. Papi mutants lacking the Tudor and auxiliary domains escape coordinated regulation by Siwi and Par-1, while another mutant lacking the auxiliary domain fails to bind piRNA precursors.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Ryuichiro Abe, Yukihiro Akeda, Noriko Sakamoto, Dan Takeuchi, Yo Sugawara, Norihisa Yamamoto, Anusak Kerdsin, Yuki Matsumoto, Daisuke Motooka, Warawut Laolerd, Pitak Santanirand, Masato Suzuki, Keigo Shibayama, Kazunori Tomono, Tetsuya Iida, Shigeyuki Hamada
Summary: The study revealed a high prevalence of Enterobacteriaceae isolates carrying bla(NDM-1) and bla(NDM-5) in clinical samples from Thailand. The dissemination of carbapenem-resistant Enterobacteriaceae (CRE) in Thailand was found to be mainly due to clonal spread of specific E. coli and Klebsiella pneumoniae clones carrying bla(NDM) genes.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Tomomi Hishinuma, Tatsuya Tada, Mari Tohya, Masaki Shintani, Masato Suzuki, Masahiro Shimojima, Teruo Kirikae
Summary: The study aimed to investigate the significance of a tandem duplicate of bla(VIM-24) in Pseudomonas aeruginosa ST1816 isolates. Thirteen isolates carrying bla(VIMs) were collected from medical settings in Japan, and four of them had a tandem duplicate of bla(VIM-24). These plasmids conferred resistance to cefepime and transferred to PAO1. Western blotting analysis showed higher production of VIM-24 metallo-beta-lactamase in strains with the tandem duplicate. This suggests that the tandem duplicate of bla(VIM-24) contributes to the proliferation of ST1816 strains in Japanese hospitals.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Microbiology
Samiratu Mahazu, Isaac Prah, Yusuke Ota, Takaya Hayashi, Yoko Nukui, Masato Suzuki, Yoshihiko Hoshino, Yukihiro Akeda, Toshihiko Suzuki, Tomoko Ishino, Anthony Ablordey, Ryoichi Saito
Summary: IncX3 and IncL plasmids play important roles in the dissemination of antibiotic resistance genes. Clinical samples from a Ghanaian hospital were used to investigate the genetic characteristics of bla(OXA-48)- and bla(OXA-181)-containing Klebsiella pneumoniae, Klebsiella quasipneumoniae, and Enterobacter cloacae. The study found that these plasmids were highly transferable and enhanced the competitive ability of host cells.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yusuke Hashimoto, Masato Suzuki, Sae Kobayashi, Yuki Hirahara, Jun Kurushima, Hidetada Hirakawa, Takahiro Nomura, Koichi Tanimoto, Haruyoshi Tomita
Summary: Linear plasmids with high self-transmissibility and the ability to acquire and maintain antimicrobial resistance (AMR) genes, particularly through transposition with IS1216E, have been identified in clinical multidrug-resistant enterococci. These plasmids play a crucial role in the spread and maintenance of AMR genes among enterococci.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Masaki Shintani, Haruo Suzuki, Hideaki Nojiri, Masato Suzuki
Summary: This study reevaluates previously published data based on the article titled 'IncP-type plasmids carrying genes for antibiotic resistance or aromatic compound degradation are prevalent in sequenced Aromatoleum and Thauera strains' by Lo et al. This correspondence aims to clarify misconceptions about plasmids classified under incompatibility (Inc) groups IncP-1 and IncP-11.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Noriko Sakamoto, Warawut Laolerd, Yukihiro Akeda, Yo Sugawara, Daisuke Motooka, Norihisa Yamamoto, Dan Takeuchi, Rathina Kumar Shanmugakani, Isao Nishi, Masato Suzuki, Keigo Shibayama, Tetsuya Iida, Pitak Santanirand, Kazunori Tomono, Shigeyuki Hamada
Summary: This study analyzed the whole-genome sequencing data of carbapenemase-producing Klebsiella pneumoniae (CPKP) isolated from a tertiary-care hospital in Bangkok, Thailand. The findings revealed the molecular epidemiology of CPKP in terms of genetic, sequence type, and phylogenetic relationships. The study identified a temporal shift in the predominant carbapenemase genes and the emergence of CPKP co-harboring multiple carbapenemase genes, indicating significant changes in CPE types in Thailand and potentially in Southeast Asian countries.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Hoang Thi An Ha, Phuong Thi Lan Nguyen, Tran Thi Mai Hung, Le Anh Tuan, Bui Thanh Thuy, Tran Hoang My Lien, Pham Duy Thai, Nguyen Ha Thanh, Vu Thi Ngoc Bich, Tran Hai Anh, Ngo Thi Hong Hanh, Nguyen Thi Minh, Duy Pham Thanh, Si-Nguyen T. Mai, Hao Chung The, Nguyen Vu Trung, Nguyen Hoai Thu, Tran Nhu Duong, Dang Duc Anh, Pham Thi Ngoc, Anne-Laure Banuls, Marc Choisy, H. Rogier van Doorn, Masato Suzuki, Tran Huy Hoang
Summary: This study investigates the prevalence of optrA positive E. faecalis (OPEfs) in 6 reservoirs in farms in Ha Nam province, Vietnam, and its associated factors, as well as explores the genetic relationship of OPEfs isolates. The highest prevalence of OPEfs was found in flies (46.8%), followed by chickens (37.3%), dogs (33.3%), humans (18.7%), wastewater (16.4%), and pigs (11.3%). The presence of OPEfs was associated with the total feeding area and total livestock unit of the farm in chickens, flies, and wastewater. Moreover, 86% of OPEfs strains were resistant to the last resort antibiotic linezolid.
Article
Multidisciplinary Sciences
Hidenori Matsu, Emiko Rimbara, Masato Suzuki, Kengo Tokunaga, Hidekazu Suzuki, Masaya Sano, Takashi Ueda, Hitoshi Tsugawa, Sohachi Nanjo, Akira Takeda, Makot Sakshi, Shuich Terao, Tsuyosh Suda, Sae Aoki, Keigo Shibayama, Hiroyosh Ota, Katsuhiro Mabe
Summary: Helicobacter suis, the most common gastric non-Helicobacter pylori Helicobacter species found in humans, has been suggested to cause many cases of gastric disease, but the transmission route from hogs remains unclear. Diagnostic methods for H. suis infection often yield negative results, making it difficult to diagnose without gastric biopsy specimens. This study introduces the use of whole-bacterial cell ELISA to simultaneously assess H. suis and H. pylori infections, with high accuracy and sensitivity.
Letter
Immunology
Masaya Sano, Emiko Rimbara, Masato Suzuki, Hidenori Matsui, Miwa Hirai, Sae Aoki, Tsuyoshi Kenri, Keigo Shibayama, Hidekazu Suzuki
EMERGING INFECTIOUS DISEASES
(2023)
Article
Veterinary Sciences
Md Amirul Hasan, Masato Suzuki, Kouji Sakai, Md Humayun Kabir, Yu Miyaoka, Hakimullah Hakim, Chisaki Kadota, Dany Shoham, Kazuaki Takehara
Summary: Full genome sequencing of two bovine RVA strains isolated in Japan in 2019 revealed rare genotype constellations and suggested their evolution through multiple reassortment events from Japanese cattle RVA strains. The emergence of these RVA strains and their reassortment with locally circulating atypical RVAs could have implications for current vaccination strategies.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2023)
Article
Biochemical Research Methods
Hidenori Matsui, Masato Suzuki, Sae Aoki, Keigo Shibayama, Kengo Tokunaga, Hidekazu Suzuki, Katsuhiro Mabe, Tsuyoshi Kenri, Emiko Rimbara
Summary: This article presents a protocol for detecting Helicobacter suis infection, including sample collection, DNA preparation, PCR, bacterial culture, and whole-genome sequencing.
Article
Medicine, Research & Experimental
Yuki Hara, Mitsutaka Iguchi, Nobuyuki Tetsuka, Hiroshi Morioka, Aki Hirabayashi, Masato Suzuki, Yuka Tomita, Keisuke Oka, Tetsuya Yagi
Summary: Carbapenemase-producing Enterobacterales (CPE) have raised concerns about infectious disease treatment options and infection control. A multicenter study conducted in Aichi Prefecture from 2015 to 2019 revealed the molecular epidemiology of CPE. The study found that CPE incidence was 0.12%, with Klebsiella pneumoniae and Enterobacter cloacae complex as the main species. The predominance of ST78 in E. cloacae complex and the spread of ST517 and ST592 in carbapenemase-producing K. pneumoniae indicate the need for strengthened infection control programs.
NAGOYA JOURNAL OF MEDICAL SCIENCE
(2022)
Article
Infectious Diseases
Ryuichiro Abe, Yukihiro Akeda, Dan Takeuchi, Noriko Sakamoto, Yo Sugawara, Norihisa Yamamoto, Anusak Kerdsin, Yuki Matsumoto, Daisuke Motooka, Warawut Leolerd, Pitak Santanirand, Masato Suzuki, Keigo Shibayama, Kazunori Tomono, Tetsuya Iida, Shigeyuki Hamada
Summary: There is a nationwide dissemination of K. pneumoniae ST16 isolates carrying bla(NDM-1) and bla(OXA-232) in Thailand, and these isolates show clonal dissemination with similar plasmids and virulence factors to highly invasive clones reported in Brazil.
JAC-ANTIMICROBIAL RESISTANCE
(2022)
