Article
Biology
Deepak Bhat, Samuel Hauf, Charles Plessy, Yohei Yokobayashi, Simone Pigolotti, Armita Nourmohammad
Summary: In this study, a new method was proposed to infer the dynamics of Replisomes from DNA abundance distribution. The method was applied to measure the DNA abundance distribution in Escherichia coli populations growing at different temperatures. The results showed that the speed of Replisomes increases with temperature and exhibits wave-like variations along the genome, which correlates with the variation of mutation rate. This study provides insights into the replication dynamics of bacteria.
Article
Multidisciplinary Sciences
Ann-Marie K. Shorrocks, Samuel E. Jones, Kaima Tsukada, Carl A. Morrow, Zoulikha Belblidia, Johanna Shen, Iolanda Vendrell, Roman Fischer, Benedikt M. Kessler, Andrew N. Blackford
Summary: The study reveals that the BTR complex contains multiple conserved motifs that interact cooperatively with the single-stranded DNA-binding protein RPA, facilitating BLM recruitment to stalled replication forks for promoting their restart after replication stress.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Liu He, Rebecca Lever, Andrew Cubbon, Muhammad Tehseen, Tabitha Jenkins, Alice O. Nottingham, Anya Horton, Hannah Betts, Martin Fisher, Samir M. Hamdan, Panos Soultanas, Edward L. Bolt
Summary: DNA strand breaks are repaired by DNA synthesis using a homologous DNA template. The DNA polymerase delta (Pol delta) catalyzes this DNA synthesis but can cause genome instability. However, the human protein HelQ inhibits DNA synthesis by Pol delta and promotes DNA strand annealing during repair. This mechanism involves the interaction between Pol delta and its POLD3 subunit with the intrinsically disordered region of HelQ.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Miri Stolovich-Rain, Ori Fridlich, Shira Azulai, Agnes Klochendler, Shira Anzi, Judith Magenheim, Ilan Stein, Fatima Mushasha, Benjamin Glaser, Eli Pikarsky, Danny Ben-Zvi, Yuval Dor
Summary: This research reveals that there is massive elimination of acinar cells during early postnatal growth of the pancreas. Measurements in the human pancreas show daily elimination of 0.7% of cells, offsetting 88% of cell formation over the first year of life. Using mouse models, it is found that cell death is associated with mitosis, where dividing cells fail to generate viable daughters. In p53-deficient mice, acinar cell death and proliferation are reduced, suggesting that p53-dependent developmental apoptosis triggers compensatory proliferation. The study suggests that excess cell turnover during organ growth allows for robustness to perturbations and supports tissue architecture maintenance.
Article
Biotechnology & Applied Microbiology
Michael A. Boemo
Summary: This paper introduces a new software, DNAscent v2, for high-throughput, single-molecule detection of DNA replication dynamics with fast and accurate detection of the thymidine analogue BrdU. The software excels in accuracy, speed, and versatility across different experimental protocols.
Article
Biochemistry & Molecular Biology
Kazutoshi Kasho, Gorazd Stojkovic, Cristina Velazquez-Ruiz, Maria Isabel Martinez-Jimenez, Mara Doimo, Timothee Laurent, Andreas Berner, Aldo E. Perez-Rivera, Louise Jenninger, Luis Blanco, Sjoerd Wanrooij
Summary: PrimPol interacts with PolDIP2 to enhance its processivity, increasing primer-template and dNTP binding affinities, and thereby enhancing nucleotide incorporation efficiency. This mechanism, involving a unique arginine cluster in PolDIP2, may be critical for PrimPol's function in tolerating DNA lesions at physiological nucleotide concentrations.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Christelle de Renty, Kelvin W. Pond, Mary K. Yagle, Nathan A. Ellis
Summary: BLM sumoylation plays a role in both normal and replication-stressed conditions. The study showed that cells with SUMO-mutant BLM exhibited defects in DNA replication, such as reduced fork restart and increased fork collapse. The SUMO-mutant BLM protein was found to be less dynamic and had a higher immobile fraction at collapsed replication forks. These findings provide important insights into the relationship between BLM sumoylation and replication stress.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Andrew Dolson, Safia Mahabub Sauty, Kholoud Shaban, Krassimir Yankulov
Summary: Dbf4-Dependent Kinase (DDK) plays an essential role in DNA replication and repair, although some of its activities are not essential for viability. Recent studies have shown that DDK also plays a key role in regulating genome-wide origin firing and replication-coupled chromatin assembly.
Article
Biochemistry & Molecular Biology
Marta San Martin Alonso, Sylvie M. Noordermeer
Summary: R-loops, RNA:DNA hybrids, play crucial roles in DNA repair processes, but excessive R-loop formation is linked to genetic instability. The multifaceted BRCA1 protein is recruited at DNA double-strand breaks to regulate R-loop removal, ensuring faithful repair processes.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Camille Henry, Gurleen Kaur, Megan E. Cherry, Sarah S. Henrikus, Nina J. Bonde, Nischal Sharma, Hope A. Beyer, Elizabeth A. Wood, Sindhu Chitteni-Pattu, Antoine M. van Oijen, Andrew Robinson, Michael M. Cox
Summary: The bacterial RecF, RecO, and RecR proteins form a closely related group involved in loading RecA protein into post-replication gaps. The targeting mechanism that brings these proteins to the appropriate gaps is unclear. However, it is proposed that RecF may interact directly with DnaN and this interaction may be involved in the targeting process. Over-expression of RecF, but not RecO or a RecF ATPase mutant, leads to replicosome destabilization and various negative effects, including loss of replicosomes, increased recombination, plasmid loss, and SOS induction. RecF is found to interact with DnaN beta-clamp and DnaG primase, and these interactions may explain the replisome effects. In vitro studies show that physiological levels of RecF can trigger post-replication gap formation. The interactions of RecF, especially with DnaN, may provide insights into how the RecFOR system is targeted to rare lesion-containing post-replication gaps, avoiding potentially deleterious RecA loading onto other replication gaps.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Oncology
Pamela S. VanderVere-Carozza, Navnath S. Gavande, Shadia I. Jalal, Karen E. Pollok, Elmira Ekinci, Joshua Heyza, Steve M. Patrick, Andi Masters, John J. Turchi, Katherine S. Pawelczak
Summary: RPA plays important roles in lung cancer as a negative prognostic biomarker and as a predictive marker for chemotherapy response. Second-generation RPA inhibitors show increased cellular permeability and exhibit anticancer activity. Chemical RPA inhibition enhances the activity of various cancer therapeutics.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Grazia Pellicano, Mohammed Al Mamun, Dolores Jurado-Santiago, Sara Villa-Hernandez, Xingyu Yin, Michele Giannattasio, Michael C. Lanz, Marcus B. Smolka, Joseph Yeeles, Katsuhiko Shirahige, Miguel Garcia-Diaz, Rodrigo Bermejo
Summary: DNA polymerase e (Pole) plays a key role in nascent strand resection, which can be prevented and stalled replication forks stabilized through checkpoint kinase regulation.
Article
Biochemistry & Molecular Biology
Anjali Mann, Miguel Angel Ramirez-Otero, Anna De Antoni, Yodhara Wijesekara Hanthi, Vincenzo Sannino, Giorgio Baldi, Lucia Falbo, Anna Schrempf, Sara Bernardo, Joanna Loizou, Vincenzo Costanzo
Summary: POLO plays an important role in repairing DNA double-strand breaks in HR-defective tumors. It is found that POLO processes stalled Okazaki fragments, preventing the accumulation of ssDNA gaps on lagging strands in the absence of RAD51. Inhibition of POLO's DNA polymerase activity leads to unprotected fork gaps, which are cleaved by the MRE11-NBS1-CtIP endonuclease, causing asymmetric single-ended DSBs that impede the survival of BRCA2-defective cells.
Article
Multidisciplinary Sciences
Sabrina F. Mansilla, Agostina P. Bertolin, Sofia Venerus Arbilla, Bryan A. Castano, Tiya Jahjah, Jenny K. Singh, Sebastian O. Siri, Maria Victoria Castro, Maria Belen de la Vega, Annabel Quinet, Lisa Wiesmueller, Vanesa Gottifredi
Summary: Recent studies have shown that the choice of a DNA damage tolerance pathway involves competition between PrimPol-mediated repriming and fork reversal. Pol t plays a unique role in regulating this pathway choice, with Pol t deficiency leading to PrimPol-dependent repriming and accelerated DNA replication. However, this excessive participation of PrimPol in DNA elongation can trigger chromosome instability and replication stress signals in Pol t-depleted cells, revealing an unexpected role of Pol t in protecting genome stability.
Article
Biochemistry & Molecular Biology
Elena A. Kouzminova, Andrei Kuzminov
Summary: Ultraviolet radiation induces chromosome fragmentation and pyrimidine dimer formation in bacteria, while RNase H deficiency may lead to the formation of R-loop-aTECs, potentially inhibiting DNA synthesis.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Engineering, Biomedical
Jung Min Yun, Sung Jin Hwang, Sung Yong Anh, Seung Min Lee, Peter Kang, Jeong Eun Lee, Eun Sil Yoon, Jin Wook Choi, Sung-Hye Park, Jung-Wook Seo, Peom Park
BIO-MEDICAL MATERIALS AND ENGINEERING
(2015)
Article
Medicine, General & Internal
Allison Baer Alley, Jeong-Wook Seo, Sung-Tae Hong
JOURNAL OF KOREAN MEDICAL SCIENCE
(2015)
Article
Cardiac & Cardiovascular Systems
Seung-Pyo Lee, Eun Seong Lee, Hongyoon Choi, Hyung-Jun Im, Youngil Koh, Min-Ho Lee, Ji-Hyun Kwon, Jin Chul Paeng, Hyung-Kwan Kim, Gi Jeong Cheon, Yong-Jin Kim, Inho Kim, Sung-Soo Yoon, Jeong-Wook Seo, Dae-Won Sohn
JACC-CARDIOVASCULAR IMAGING
(2015)
Article
Medicine, General & Internal
Ajay Chaurasia, Sung-Hye Park, Jeong-Wook Seo, Chul-Kee Park
JOURNAL OF KOREAN MEDICAL SCIENCE
(2016)
Article
Multidisciplinary Sciences
Jeong-Wook Seo, Hosik Chung, Jungmin Yun, Jin Young Park, Eunsun Park, Yuri Ahn
Article
Cardiac & Cardiovascular Systems
Jae Gun Kwak, Jeong-Wook Seo, Sam Se Oh, Sang Yun Lee, Eui Keun Ham, Woong-Han Kim, Soo-Jin Kim, Eun Jung Bae, Cheoung Lim, Chang-Ha Lee, Cheul Lee
CARDIOVASCULAR PATHOLOGY
(2014)
Article
Medicine, General & Internal
Hwa Jin Cho, Hanna Kim, Yoon Jin Kwak, Jeong Wook Seo, Sun Ha Paek, Chul-Ho Sohn, Jung Min Yun, Da Seu Ran Kim, Peter Kang, Peom Park, Sung-Hye Park
JOURNAL OF KOREAN MEDICAL SCIENCE
(2014)
Article
Medicine, General & Internal
So-Ryoung Lee, Youngjin Cho, Myung-jin Cha, Eue-Keun Choi, Jeong-Wook Seo, Seil Oh
JOURNAL OF KOREAN MEDICAL SCIENCE
(2018)
Article
Cardiac & Cardiovascular Systems
Myung-Jin Cha, Jeong-Wook Seo, Hak Jae Kim, Moo-kang Kim, Hye-sun Yoon, Seong Won Jo, Seil Oh, Ji Hyun Chang
Summary: High-dose cardiac radioablation induced mononuclear cell-related intracellular and extracellular edema, diffuse vacuolization, and widened intercalated discs in rat hearts within 1 month. ECG showed intracardiac conduction delay and significant ST-segment changes. These findings suggest that early antiarrhythmic effects following cardiac radioablation are due to conduction disturbances and membrane potential alterations, rather than necrosis.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Cardiac & Cardiovascular Systems
Mi Kyoung Song, Sang Yoon Lee, Soon Ho Yoon, Jeong-Wook Seo
Summary: This study investigated the clinical value of patient-specific 3D models in multidisciplinary discussions for patients with complex congenital heart defects (CHDs). The results showed that the use of 3D-printed models improved the understanding of the disease, facilitated multidisciplinary discussions, and enhanced surgical decision-making.
JOURNAL OF CARDIAC SURGERY
(2023)
Article
Medicine, General & Internal
Sangjoon Choi, SangHan Lee, Jeong-Wook Seo, Min-ju Kim, Yo Han Jeon, Ji Hyun Park, Jong Kyu Lee, Nam Seok Yeo
Summary: Autopsy findings of a 22-year-old man who died 7 hours after developing chest pain 5 days post BNT162b2 mRNA vaccine showed isolated atrial myocarditis with no evidence of microthrombosis or infection in the heart and other organs. Histological examination revealed necrosis of myocytes and extensive contraction band necrosis. The primary cause of death was determined to be myocarditis causally-associated with the BNT162b2 vaccine.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2021)
Review
Communication
Jeong-Wook Seo, Hosik Chung, Tae-Sul Seo, Youngim Jung, Eun Seong Hwang, Cheol-Heui Yun, Hyungsun Kim
Meeting Abstract
Hematology
H. Park, J-W Kim, Y. Koh, J-O Lee, K. H. Kim, S-M Bang, I Kim, J. Lee, S-S Yoon, J-W Seo, D-W Sohn, S. Park
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2015)
Article
Communication
Viera Wardhani, Sam Mathew, Jeong-Wook Seo, Komang G. Wiryawan, Vivi Setiawaty, Burmaajav Badrakh
Review
Cardiac & Cardiovascular Systems
Il-Young Oh, Myung-Jin Cha, Tae-Hui Lee, Jeong-Wook Seo, Seil Oh
KOREAN CIRCULATION JOURNAL
(2018)