Article
Biochemistry & Molecular Biology
Zhen Sun, Yuan Lin, Mohammed T. Islam, Richard Koche, Lin Hedehus, Dingyu Liu, Chang Huang, Thomas Vierbuchen, Charles L. Sawyers, Kristian Helin
Summary: Nuclear receptor-binding SET-domain protein 1 (NSD1) is a crucial methyltransferase involved in transcriptional regulation and is dysregulated in diseases like Sotos syndrome. NSD1 associates with H3K36me2 at regulatory elements, particularly enhancers. It promotes enhancer-dependent gene transcription by facilitating RNA polymerase II pause release, affecting cell fate transition and Sotos syndrome development.
Article
Biochemistry & Molecular Biology
Shusei Mori, Satoyo Oya, Mayumi Takahashi, Kazuya Takashima, Soichi Inagaki, Tetsuji Kakutani
Summary: Based on studies of the plant genome, it has been found that the demethylation of H3K4me2 plays a negative regulatory role in transcriptionally active genes. A previous study identified a key demethylase LDL3, which interacts with RNA polymerase II and RNA transcription elongation factors to demethylate H3K4me2. These findings suggest that H3K4me2 demethylation serves as a chromatin record of transcriptional activity in plants, ensuring robust gene control.
Article
Plant Sciences
Weijie Zhao, Xiaoyan Wang, Qian Zhang, Qian Zheng, Haitao Yao, Xiangyang Gu, Dongliang Liu, Xuemin Tian, Xiaoji Wang, Yongqing Li, Zhengge Zhu
Summary: This study reports the important role of histone demethylase JMJ710 in the response to drought stress in rice. JMJ710 negatively regulates the expression of MYB48-1 by interacting with MYB48-1 chromatin and demethylating H3K36me2, resulting in the activation of downstream drought-responsive genes and enhanced drought tolerance. JMJ710 is downregulated under drought stress, leading to increased expression of MYB48-1 and subsequent activation of drought-responsive genes.
Article
Multidisciplinary Sciences
Haipeng Guan, Pei Wang, Pei Zhang, Chun Ruan, Yutian Ou, Bo Peng, Xiangdong Zheng, Jianlin Lei, Bing Li, Chuangye Yan, Haitao Li
Summary: The Rpd3S complex recognizes and deacetylates histones H3 and H4 at transcribed regions, guided by H3K36me3 modifications. Cryo-electron microscopy structures of Rpd3S in free and H3K36me3 nucleosome-bound states reveal a unique architecture. Multivalent recognition of H3K36me3 marks, nucleosomal DNA, and linker DNAs positions the catalytic center for deacetylation. Combinatorial readout of unmethylated H3K4 and H3K36me3 directs specific histone H3 deacetylation.
Article
Genetics & Heredity
Shalini Guha, Sukesh R. Bhaumik
Summary: The cell has various mechanisms to protect the integrity of the genomic DNA, and transcription-coupled repair plays a crucial role in maintaining the integrity of the active genome.
Article
Biochemistry & Molecular Biology
Ryan J. Separovich, Mandy W. M. Wong, Tara K. Bartolec, Joshua J. Hamey, Marc R. Wilkins
Summary: This study comprehensively analyzed the phosphoregulation of the histone methylation network in Saccharomyces cerevisiae. The functional investigation revealed the important roles of phosphorylation sites on several enzymes in cellular stress responses.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Review
Genetics & Heredity
E. Lesage, T. Clouaire, G. Legube
Summary: DNA double-strand breaks are toxic lesions triggered by environmental sources and physiological processes, especially in transcriptionally active genomic loci. Recent research suggests that these breaks are repaired through specific pathways involving transcriptional repression, chromatin signaling, RNA species, and DSB mobility.
Article
Biochemistry & Molecular Biology
Haifen Chen, Bo Hu, Cynthia Horth, Eric Bareke, Phillip Rosenbaum, Sin Young Kwon, Jacinthe Sirois, Daniel N. Weinberg, Faith M. Robison, Benjamin A. Garcia, Chao Lu, William A. Pastor, Jacek Majewski
Summary: Epigenetic modifications on the chromatin do not occur in isolation, but form a highly interconnected network. Deletion of one component, such as the histone 3 lysine 36 methyltransferase NSD1, can cause a major reorganization of the entire system. This study highlights the importance of H3K36me2 as a determinant of the developmental epigenome and provides insights into the interactions within the H3K27-H3K36-DNA methylation subnetwork.
Review
Oncology
Jiaxin Liu, Jia-nan Li, Hongyu Wu, Panpan Liu
Summary: Epigenetic regulation plays a crucial role in lymphoma development and is closely associated with various malignancies. Understanding these epigenetic mechanisms can guide the development of clinical treatments.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Marit E. Geijer, Di Zhou, Kathiresan Selvam, Barbara Steurer, Chirantani Mukherjee, Bastiaan Evers, Simona Cugusi, Marvin van Toorn, Melanie van der Woude, Roel C. Janssens, Yannick P. Kok, Wenzhi Gong, Anja Raams, Calvin S. Y. Lo, Joyce H. G. Lebbink, Bart Geverts, Dalton A. Plummer, Karel Bezstarosti, Arjan F. Theil, Richard Mitter, Adriaan B. Houtsmuller, Wim Vermeulen, Jeroen A. A. Demmers, Shisheng Li, Marcel A. T. M. van Vugt, Hannes Lans, Rene Bernards, Jesper Q. Svejstrup, Arnab Ray Chaudhuri, John J. Wyrick, Jurgen A. Marteijn
Summary: Correct transcription is crucial for life, and cells have intricate mechanisms to counteract transcription-blocking lesions. The elongation factor ELOF1 plays an important role in the transcription stress response following DNA damage, protecting the transcription machinery via two distinct mechanisms.
NATURE CELL BIOLOGY
(2021)
Review
Cell Biology
Aline Marnef, Gaelle Legube
Summary: R-loops have dual consequences for gene expression and genome stability; they accumulate near DSBs and may affect DSB repair processes.
NATURE CELL BIOLOGY
(2021)
Article
Plant Sciences
Han Chen, Yujie Fang, Wenrui Song, Haidong Shu, Xi Li, Wenwu Ye, Yuanchao Wang, Suomeng Dong
Summary: In this study, a highly induced PsKMT3 gene was found to play an important role in regulating reproductive development and pathogenicity in Phytophthora sojae. The deletion of PsKMT3 resulted in asexual development and pathogenicity defects. The study also revealed that histone H3K36 trimethylation was significantly reduced and involved in the activation of RxLR effector genes.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Cell Biology
Toshiro Iwagawa, Masaya Fukushima, Shigeru Takeuchi, Yuichi Kawamura, Yuko Aihara, Manabu Ozawa, Nayuta Yakushiji-Kaminatsui, Makoto Aihara, Haruhiko Koseki, Yutaka Suzuki, Sumiko Watanabe
Summary: This study investigated the role of histone H3K36 methylation in retinal development by analyzing the loss-of-function of H3K36me1/2 demethylases Fbxl10 and Fbxl11. The results showed that knockout of Fbxl10 in the developing retina did not result in developmental abnormalities, while knockout of Fbxl11 in the developing retina increased apoptosis, suppressed the proliferation of retinal progenitor cells, and resulted in microphthalmia. The study also found that Fbxl11 knockout altered gene splicing and the distribution of H3K36me2/3. Overall, Fbxl11 plays a pivotal role in the development of late-born retinal cell types and regulates H3K36 methylation during retinal development.
Review
Biochemistry & Molecular Biology
Xiancan Wang, Xianghai Kong, Xin Feng, Ding-Sheng Jiang
Summary: Ferroptosis is a type of programmed cell death characterized by increased levels of ferrous ions and lipid peroxidation. It has been found to play a role in various organ injuries and degenerative diseases, while insufficient ferroptosis has been linked to tumorigenesis. The regulatory mechanisms of ferroptosis involve iron metabolism, redox systems, and epigenetic mechanisms. This review provides a critical analysis of the molecular mechanisms and regulatory networks of ferroptosis, with a focus on the role of DNA, RNA, and protein methylation. The unanswered questions and debated findings in this field are also discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Oncology
Hsiangyu Hu, Nirmalya Saha, Yuting Yang, Ejaz Ahmad, Lauren Lachowski, Uttar Shrestha, Vidhya Premkumar, James Ropa, Lili Chen, Blaine Teahan, Sierrah Grigsby, Rolf Marschalek, Zaneta Nikolovska-Coleska, Andrew G. Muntean
Summary: This study reveals the importance of the YEATS domain in MLL-ENL fusion protein-mediated leukemogenesis. The YEATS domain is essential for leukemic cell growth and for the binding of the MLL-ENL fusion protein to pro-leukemic genes. Targeting the YEATS domain with inhibitors could be a potential therapeutic strategy for t(11;19) patients.
Review
Hematology
Denis F. Noubouossie, Brandi N. Reeves, Brian D. Strahl, Nigel S. Key
Article
Biochemistry & Molecular Biology
Katherine I. Albanese, Mackenzie W. Krone, Christopher J. Petell, Madison M. Parker, Brian D. Strahl, Eric M. Brustad, Marcey L. Waters
ACS CHEMICAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Hashem A. Meriesh, Andrew M. Lerner, Mahesh B. Chandrasekharan, Brian D. Strahl
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Cell & Tissue Engineering
Michael J. Chen, Edroaldo Lummertz da Rocha, Patrick Cahan, Caroline Kubaczka, Phoebe Hunter, Patricia Sousa, Nathaniel K. Mullin, Yuko Fujiwara, Minh Nguyen, Yuqi Tan, Yi Zhou, Trista E. North, Leonard Zon, George Q. Daley, Thorsten M. Schlaeger
Article
Chemistry, Medicinal
Anne-Marie W. Turner, Raghuvar Dronamraju, Frances Potjewyd, Katherine S. James, Daniel K. Winecoff, Jennifer L. Kirchherr, Nancie M. Archin, Edward P. Browne, Brian D. Strahl, David M. Margolis, Lindsey James
ACS INFECTIOUS DISEASES
(2020)
News Item
Biochemistry & Molecular Biology
Kanishk Jain, Brian D. Strahl
Summary: High-throughput biochemical and biological analyses of disease-associated histone mutations reveal key residues in globular cores that affect chromatin remodeling, nucleosome stability, and stem cell pluripotency.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrew R. Yoblinski, Seoyoung Chung, Sophie B. Robinson, Kaitlyn E. Forester, Brian D. Strahl, Raghuvar Dronamraju
Summary: In yeast cells, Dma1 and Dma2 work redundantly and catalytically to maintain genome stability, influencing the choice of repair pathways. They localize to transcribed regions of the genome and increase in abundance after DNA damage.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Paul Enriquez, Krzysztof Krajewski, Brian D. Strahl, Scott B. Rothbart, Robert H. Dowen, Robert B. Rose
Summary: This study explores the function of Bromodomains in SWI/SNF chromatin-remodeling complexes, demonstrating the recognition of acetylated lysine 14 on histones by the SMARCA4 BD. The findings suggest essential roles for the SWI/SNF bromodomain in vivo and reveal potential redundancy in epigenetic readers regulating chromatin remodeling. Implications include the development of small-molecule BD inhibitors for cancer and disease treatment.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Jibo Zhang, Aakanksha Gundu, Brian D. Strahl
Summary: In this study, Zhang et al. investigate how transcription programs rapidly adjust to changing metabolic and cellular cues, with a focus on the role of the Yaf9 component in maintaining timely transcription of metabolic genes across the yeast metabolic cycle. They find that Yaf9 recruits chromatin regulatory complexes to deposit H2A.Z and acetylate H4, promoting transcriptional initiation during the oxidative phase. These findings suggest a dynamic chromatin and transcription initiation factor signature is necessary for proper regulation of metabolic gene transcription during the yeast metabolic cycle, with unique regulatory mechanisms at distinct metabolic states.
GENES & DEVELOPMENT
(2021)
Article
Biology
Christopher J. Petell, Kathyrn Randene, Michael Pappas, Diego Sandoval, Brian D. Strahl, Joseph S. Harrison, Joshua P. Steimel
Summary: The METRIS assay leverages the concept of friction to measure protein-protein interaction affinities, offering high resolution and sensitivity. It allows for the measurement of a wide range of affinities with a small amount of reagents, providing new insights into epigenetic recognition.
Article
Genetics & Heredity
Aaron T. Crain, Stephen Klusza, Robin L. Armstrong, Priscila Santa Rosa, Brenda R. S. Temple, Brian D. Strahl, Daniel J. McKay, A. Gregory Matera, Robert J. Duronio
Summary: KMT5A and Set8 enzymes have functional conservation in Drosophila development, and the N-terminal domain of Set8 has a specific role in eye development, which is distinct from H4K20me1.
Editorial Material
Cell Biology
Kanishk Jain, Brian D. Strahl
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Letter
Cell Biology
Zhenzhen Chen, Taylor Lundy, Zhongliang Zhu, Victoria E. Hoskins, Jiahai Zhang, Xuebiao Yao, Brian D. Strahl, Chao Xu
Correction
Cell Biology
Zhenzhen Chen, Taylor Lundy, Zhongliang Zhu, Victoria E. E. Hoskins, Jiahai Zhang, Xuebiao Yao, Brian D. D. Strahl, Chao Xu
Article
Multidisciplinary Sciences
Adam H. Tencer, Khan L. Cox, Gregory M. Wright, Yi Zhang, Christopher J. Petell, Brianna J. Klein, Brian D. Strahl, Joshua C. Black, Michael G. Poirier, Tatiana G. Kutateladze
NATURE COMMUNICATIONS
(2020)
