Article
Microbiology
Kevin K. Takaki, Gabriel Rinaldi, Matthew Berriman, Antonio J. Pagan, Lalita Ramakrishnan
Summary: Schistosome eggs and beads can induce granulomatous response, but only mature Schistosoma mansoni eggs recruit macrophages to form granulomas, facilitating egg extrusion. This indicates the crucial role of the eggshell in immunity.
CELL HOST & MICROBE
(2021)
Article
Biochemistry & Molecular Biology
Risoleta Nogueira Soares, Eulalia Camelo Pessoa de Azevedo Ximenes, Sidcley Bernardino Araujo, Romildo Luciano da Silva, Valdenia Maria Oliveira de Souza, Luana Cassandra Breitenbach Barroso Coelho, Jose Lourenco de Freitas Neto, Pedro Jose Rolim Neto, Hallysson Douglas Andrade de Araujo, Andre de Lima Aires, Monica Camelo Pessoa de Azevedo Albuquerque
Summary: In this study, the in vivo effect of 8-lapachone complexed with methyl-8-cyclodextrin (M8CD) on the stages of Schistosoma mansoni in a murine model was evaluated. The results showed that 8-lap:M8CD significantly reduced the number of worms and the damage caused by eggs, possibly through reducing the granuloma size.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Infectious Diseases
Biruk Kassa, Michael B. Lee, Rahul H. Kumar, Claudia A. Mickael, Linda H. Sanders, Rubin A. Tuder, Margaret H. Mentink-Kane, Brian A. Graham
Summary: This study found that Schistosoma japonicum can also cause experimental pulmonary hypertension, but the severity is milder than that caused by Schistosoma mansoni. This difference may result from the distribution of eggs in the lungs and is independent of which species is used for sensitization. This finding is consistent with the clearer association between S. mansoni infection and the development of schistosomiasis-associated PAH in humans.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Microbiology
Yi-Ting Yeh, Danielle E. Skinner, Ernesto Criado-Hidalgo, Natalie Shee Chen, Antoni Garcia-De Herreros, Nelly El-Sakkary, Lawrence Liu, Shun Zhang, Adithan Kandasamy, Shu Chien, Juan C. Lasheras, Juan C. del Alamo, Conor R. Caffrey
Summary: This study reveals that the eggs of the blood fluke Schistosoma induce vascular endothelial cells (VECs) to encapsulate them and form membrane extensions for communication. Live eggs stimulate cell contractility and cause significant mechanical deformations during encapsulation. These findings improve our understanding of the interaction between the parasite and the host and its role in disease transmission.
Article
Biochemistry & Molecular Biology
Vinicius Gustavo de Oliveira, Vanessa Fernandes Rodrigues, Joao Marcelo Peixoto Moreira, Jailza Lima Rodrigues, Laura Maggi, Samira Diniz Resende, Deborah Negrao-Correa
Summary: The severity of chronic schistosomiasis is associated with the inflammation induced by egg-secreted antigens. Eosinophils play a role in the pathology of schistosomiasis, and their absence leads to changes in liver immune response and tissue damage.
Article
Biochemistry & Molecular Biology
Lucas M. N. Silva, Wilza W. M. Franca, Victor H. B. Santos, Renan A. F. Souza, Adriana M. Silva, Emily G. M. Diniz, Thierry W. A. Aguiar, Joao V. R. Rocha, Mary A. A. Souza, Wheverton R. C. Nascimento, Reginaldo G. Lima Neto, Iranildo J. Cruz Filho, Eulalia C. P. A. Ximenes, Hallysson D. A. Araujo, Andre L. Aires, Monica C. P. A. Albuquerque
Summary: This study evaluated the schistosomicidal potential of Plumbagin (PLUM) against Schistosoma mansoni and its pharmacokinetic parameters. The results showed that PLUM significantly reduced the worm and egg load, as well as demonstrated good pharmacokinetic profiles in silico.
Article
Parasitology
Clenio Silva Cruz, Wilza Wanessa Melo Franca, Hallysson Douglas Andrade de Arujo, Eulalia Camelo Pessoa Azevedo Ximenes, Valdenia Maria de Souza, Monica Camelo Pessoa Azevedo Albuquerque, Andre Lima Aires, Vlaudia Maria Assis Costa
Summary: Studies have shown that B. clausii can improve parasitological parameters in experimental Schistosoma mansoni infections, but it does not have a significant effect on killing adult worms.
Article
Microbiology
Bruna Dias de Lima Fragelli, Ana Carolina Maragno Fattori, Elisandra de Almeida Montija, Joice Margareth de Almeida Rodolpho, Cynthia Aparecida de Castro, Krissia Franco de Godoy, Camila Tita Nogueira, Vanderlei Rodrigues, Edson Garcia Soares, Larissa Romanello, Juliana R. Torini, Humberto D'Muniz Pereira, Fernanda de Freitas Anibal
Summary: Schistosomiasis is a parasitic infection that affects over 230 million people worldwide, causing 200,000 deaths annually. The study evaluated the effects of recombinant enzymes in the immunotherapy of schistosomiasis in a murine model. The results showed that the enzyme HGPRT stimulated the production of IL-4 and reduced the number of granulomas in the liver, while the enzyme PNP and the MIX reduced the number of worms and eggs, and modulated eosinophil count.
Article
Biology
Kassia K. Malta, Thiago P. Silva, Cinthia Palazzi, Vitor H. Neves, Livia A. S. Carmo, Simone J. Cardoso, Rossana C. N. Melo
Summary: This article explores the hepatic Schistosoma mansoni granuloma as a real ecosystem, composed of cells, protein scaffolds, fibers, and chemical compounds, with complex energy and matter inputs and outputs. The granuloma is considered as a multifaceted cell community that undergoes progressive changes in its composition over time, highlighting the importance of understanding schistosomiasis.
BIOLOGICAL REVIEWS
(2021)
Article
Plant Sciences
Joao Gustavo Mendes Rodrigues, Paula Sibelly Veras Albuquerque, Johnny R. Nascimento, Jaianna Andressa Viana Campos, Andressa S. S. Godinho, Sulayne Janayna Araujo, Jefferson Mesquita Brito, Caroline M. Jesus, Guilherme Silva Miranda, Michelle C. Rezende, Deborah Aparecida Negrao-Correa, Claudia Q. Rocha, Lucilene Amorim Silva, Rosane N. M. Guerra, Flavia R. F. Nascimento
Summary: Chenopodium ambrosioides extract showed antiparasitic and immunomodulatory effects by increasing the mortality of schistosoma in vitro, reducing eggs in feces and liver in vivo, decreasing liver granulomas, and improving cellular immune response.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Immunology
Cindy Reinholdt, Franziska Winkelmann, Nicole Koslowski, Emil C. Reisinger, Martina Sombetzki
Summary: Long-term unisexual schistosome infection leads to an unpolarized Th1/Th2 immune response and organ inflammation. This study shows that single schistosomes can mate and lay eggs months after infection, resulting in Typ2 immune response and fibrosis. Therefore, infection status without positive egg detection is still important.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Parasitology
A. F. Atia, N. M. Abou-Hussien, D. M. Sweed, E. Sweed, N. A. Abo-khalil
Summary: This study assessed the effectiveness of auranofin in treating hepatic granuloma and fibrogenesis caused by Schistosoma mansoni eggs. The results showed that auranofin significantly reduced parasite burden and egg presence, decreased granuloma number and size, and alleviated fibrosis. Therefore, auranofin holds potential as a medication for the treatment of schistosomiasis.
JOURNAL OF HELMINTHOLOGY
(2023)
Article
Biochemical Research Methods
Katja R. Wiedemann, Alejandra Peter Ventura, Stefanie Gerbig, Martin Roderfeld, Thomas Quack, Christoph G. Grevelding, Elke Roeb, Bernhard Spengler
Summary: This study identified and studied infection markers in the livers of hamsters infected with the parasitic Schistosoma mansoni using LC-MS/MS technology, and matched these markers to specific anatomical structures using high-resolution MALDI MSI.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Infectious Diseases
Zhidan Li, Xiaoling Wang, Wei Zhang, Wenbin Yang, Bin Xu, Wei Hu
Summary: This study found that excretory/secretory products (ESPs) derived from schistosome eggs could effectively alleviate allergic airway inflammation, potentially through the upregulation of regulatory T cells (Tregs). Furthermore, potential therapeutic proteins against allergic inflammation were identified in these ESPs.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Laura Maggi, Genil Mororo Araujo Camelo, Izabella Chrystina Rocha, William Pereira Alves, Joao Marcelo Peixoto Moreira, Thiago Almeida Pereira, Wagner Luiz Tafuri, Elida Mara Leite Rabelo, Jr Ary Correa, Roselene Ecco, Deborah Aparecida Negrao-Correa
Summary: S. mansoni eggs induce cytokine release and granuloma formation, which is inhibited by IL-33/ST2 pathway. Disruption of this pathway results in disorganized granulomas and reduced collagen formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Pasi K. Korhonen, Liina Kinkar, Neil D. Young, Huimin Cai, Marshall W. Lightowlers, Charles Gauci, Abdul Jabbar, Bill C. H. Chang, Tao Wang, Andreas Hofmann, Anson Koehler, Junhua Li, Jiandong Li, Daxi Wang, Jiefang Yin, Huanming Yang, David J. Jenkins, Urmas Saarma, Teivi Laurimae, Mohammad Rostami-Nejad, Malik Irshadullah, Hossein Mirhendi, Mitra Sharbatkhori, Francisco Ponce-Gordo, Sami Simsek, Adriano Casulli, Houria Zait, Hripsime Atoyan, Mario Luiz de la Rue, Thomas Romig, Marion Wassermann, Sargis A. Aghayan, Hasmik Gevorgyan, Bicheng Yang, Robin B. Gasser
Summary: This article presents advances in the study of the genomic organization of cystic echinococcosis, including the development of vaccines and exploration of their functions. A chromosomal-scale genome for the parasite was assembled, and the EG95 gene family members were localized within this genome. The conservation of the EG95 vaccine molecule was evaluated. These findings have significant implications for the improvement of diagnosis and chemotherapy for cystic echinococcosis.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Medicinal
Shahid Iqbal, Fang Ge, Fuyi Li, Tatsuya Akutsu, Yuanting Zheng, Robin B. Gasser, Dong-Jun Yu, Geoffrey Webb, Jiangning Song
Summary: PROST is a sequence-based predictor for protein stability changes caused by single-point missense mutations. It utilizes various sequence-based features, physicochemical properties, evolutionary information, and predicted structural features to accurately predict the protein stability changes. The performance of PROST is evaluated on multiple datasets and compared with state-of-the-art predictors, demonstrating its superiority.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Biochemistry & Molecular Biology
Xindong Xu, Yifeng Wang, Changhong Wang, Gangqiang Guo, Xinyu Yu, Yang Dai, Yaobao Liu, Guiying Wei, Xiaohui He, Ge Jin, Ziqiu Zhang, Qingtian Guan, Arnab Pain, Shengyue Wang, Wenbao Zhang, Neil D. Young, Robin B. Gasser, Donald P. McManus, Jun Cao, Qi Zhou, Qingfeng Zhang
Summary: This study reports a chromosome-level reference genome for Schistosoma japonicum, shedding light on the evolution of sex chromosomes and providing insights into the sex determination and differentiation of this species. The study also identified W-linked genes and female-preferentially transcribed genes, which play critical roles in the maturation of female worms.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Ecology
Joshua A. Thia, Pasi K. Korhonen, Neil D. Young, Robin B. Gasser, Paul A. Umina, Qiong Yang, Owain Edwards, Tom Walsh, Ary A. Hoffmann
Summary: Genomic data provides insights into pest management, resistance evolution, pest invasions, and population dynamics. The first reference genome for the redlegged earth mite, Halotydeus destructor, was assembled to study pesticide adaptation and population demographics using whole-genome pool-seq data. Genetic mutations have been identified that contribute to organophosphate and pyrethroid resistance. Population genomic analyses reveal regional structuring in H. destructor populations, while demographic analyses indicate reduced genetic diversity and smaller effective population sizes in eastern populations. These findings have important implications for genetic markers, understanding resistance mechanisms, and predictive modeling of resistance evolution and spread.
JOURNAL OF EVOLUTIONARY BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Liina Kinkar, Pasi K. Korhonen, Urmas Saarma, Tao Wang, Xing-Quan Zhu, Ivon Harliwong, Bicheng Yang, J. Lynn Fink, Daxi Wang, Bill C. H. Chang, Galina N. Chelomina, Anson V. Koehler, Neil D. Young, Robin B. Gasser
Summary: Clonorchis sinensis shows significant genetic variation between populations in China and Far East Russia, which may have distinct epidemiology, pathogenicity, and chemotherapeutic responsiveness.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Food Science & Technology
Barana Hewa Nadugala, Rick Hantink, Tom Nebl, Jacinta White, Charles N. Pagel, C. S. Ranadheera, Amy Logan, Jared K. Raynes
Summary: To explore the functions of glycosylation in bovine milk, the glycosylated and unglycosylated forms of kappa-Casein B were purified and analyzed for their amyloid fibril formation, morphology, oligomerisation states, and protein structure. It was found that the two forms of kappa-Casein B had similar size aggregates, but the glycosylated form had a higher amyloid fibril formation rate and different fibril morphology compared to the unglycosylated form. Glycosylation did not affect the polarity or interactions between protein molecules in the native form, but it did influence the protein folding patterns and fluorescence intensity. Overall, these results highlight the importance of glycosylation in the modulation of aggregation states and formation of amyloid fibrils from intrinsically disordered proteins.
CURRENT RESEARCH IN FOOD SCIENCE
(2023)
Article
Biochemical Research Methods
Yan Liu, Guo Wei, Chen Li, Long-Chen Shen, Robin B. Gasser, Jiangning Song, Dijun Chen, Dong-Jun Yu
Summary: Single-cell RNA sequencing (scRNA-seq) has greatly facilitated the characterization of cell lineages and types in complex tissues and organisms. Cell-type annotation is important in scRNA-seq analysis, but manual annotation can be time-consuming and subjective. To address these challenges, we propose a new pipeline called TripletCell for cross-species, cross-protocol, and cross-sample cell-type annotation. Experimental results demonstrate that TripletCell outperforms existing methods and can accurately annotate different cell types regardless of protocols or species.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Plant Sciences
Chen Zhang, Kah Yean Lum, Aya C. Taki, Robin B. Gasser, Joseph J. Byrne, Luis J. Montaner, Ian Tietjen, Vicky M. Avery, Rohan A. Davis
Summary: The study focused on the purification and synthesis of a bioactive compound derived from Eremophila microtheca. A library of carbamate-based compounds was generated using the synthesized compound as a scaffold, and their activities against parasites, malaria, and HIV were evaluated. The results showed promising antiparasitic, antimalarial, and antiviral activities of certain compounds.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Nidhish Francis, Reza Sanaei, Babatunde A. Ayodele, Neil M. O'Brien-Simpson, David P. Fairlie, Lakshmi C. Wijeyewickrema, Robert N. Pike, Eleanor Jean Mackie, Charles Neil Pagel
Summary: The study found that administration of PAR(2) antagonist GB88 can reduce periodontitis and alveolar bone resorption caused by Porphyromonas gingivalis, possibly by inhibiting inflammatory response.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biology
Fuyi Li, Xudong Guo, Yue Bi, Runchang Jia, Miranda E. Pitt, Shirui Pan, Shuqin Li, Robin B. Gasser, Lachlan JM. Coin, Jiangning Song
Summary: A deep learning-based approach called Digerati was developed for the rapid and accurate identification of PE and PPE family proteins. Digerati achieved a significantly better performance (-18-20%) than alignment-based methods. It is expected to facilitate high-throughput identification and analysis of PE/PPE family members.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Yuanting Zheng, Neil D. Young, Jiangning Song, Robin B. Gasser
Summary: Biodiversity within the animal kingdom is associated with extensive molecular diversity. The expansion of genomic, transcriptomic, and proteomic data sets for invertebrate groups necessitates reliable in silico tools for molecular identification. This study established a sequence- and structure-based workflow system to identify and annotate proteases and protease inhibitors of a highly pathogenic parasitic roundworm. The results provide a solid foundation for further exploration of these molecules and their potential as drug targets. Rating: 8/10.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lucas G. Huggins, Vito Colella, Neil D. Young, Rebecca J. Traub
Summary: Apicomplexan haemoparasites cause significant morbidity and mortality, and traditional diagnostic methods have limitations. The nanopore sequencing method can accurately detect multiple pathogens, improving taxonomic resolution.
MOLECULAR ECOLOGY RESOURCES
(2023)
Editorial Material
Parasitology
Bernd H. Kalinna
Summary: The study identified genomic safe harbour (GSH) sites in Schistosoma mansoni and inserted a transgene using CRISPR/Cas9-assisted homology-directed repair, providing a promising strategy for functional genomics research on this neglected tropical parasite.
TRENDS IN PARASITOLOGY
(2023)
Article
Biochemical Research Methods
Tong Pan, Chen Li, Yue Bi, Zhikang Wang, Robin B. Gasser, Anthony W. Purcell, Tatsuya Akutsu, Geoffrey Webb, Seiya Imoto, Jiangning Song
Summary: PFresGO is an attention-based deep-learning approach that incorporates hierarchical structures in Gene Ontology (GO) graphs and natural language processing algorithms for functional annotation of proteins, achieving superior performance compared to existing methods.