Article
Chemistry, Multidisciplinary
Ye Zhou, Jannik Nedergaard Pedersen, Jacob Nedergaard Pedersen, Nykola C. Jones, Soren Vronning Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Renjun Gao, Zheng Guo
Summary: This study focuses on the anionization of a carboxylate-enriched mutant of Bacillus subtilis lipase A (8M) to produce biofluids with enhanced hydrolytic activity. The resulting biofluids show a 2.5-fold increase in activity compared to those based on anionic polymer surfactants. Additionally, the use of anion-type biofluids with Myoglobin (Mb) is evaluated, revealing partial recovery of the active alpha-helix level in the biofluids. These highly active solvent-free liquid enzymes exhibit increased thermal stability and open up new possibilities in solvent-free liquid protein research.
Article
Chemistry, Physical
Helena D. D. Tjornelund, Jesper Brask, John M. M. Woodley, Gunther H. J. Peters
Summary: In this study, an optimized protocol for controlling the initial water activity in organic reaction mixtures with soluble lipase was proposed. The protocol ensures reproducible and consistent enzyme activity measurements. The importance of consistent stirring and volume correction to compensate for solvent evaporation was highlighted. The study also tested the protocol on soluble and immobilized lipase and found similar water activity profiles and the highest enzyme activity in hexane. This optimized protocol expands the possibilities for studying enzyme kinetics.
Article
Biotechnology & Applied Microbiology
Priyanka V. Jawale, Bhalchandra M. Bhanage
Summary: The study focused on analyzing reaction kinetics and mechanism for synthesizing propyl benzoate in solvent-free conditions, immobilizing lipase on HPMC and PVA polymer blend, with CCL showing excellent activity. Optimizing reaction conditions and calculating kinetic parameters revealed that propyl benzoate synthesis using immobilized CCL followed the ternary complex model.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Chemistry, Applied
Siyu Zhang, Joseph R. Hyatt, Casimir C. Akoh
Summary: In this study, a multi-functional galloylated structured lipid DOGG was successfully synthesized using immobilized food grade Candida antarctica lipase B. The reaction conditions were optimized and the structure of the product was confirmed. The enzyme showed good reusability and DOGG has potential applications as an antioxidant and nutraceutical.
Review
Polymer Science
Hong Wang, Hongpeng Li, Chee Keong Lee, Noreen Suliani Mat Nanyan, Guan Seng Tay
Summary: This article presents the reaction modes of enzyme-catalyzed polyester synthesis and the mechanism of lipase-catalyzed polymerization. It also discusses the advantages and disadvantages of enzyme-catalyzed polyester synthesis under different solvent systems. Furthermore, it examines the challenges for further industrialization of this method.
Article
Biology
Supajit Sraphet, Bagher Javadi
Summary: This paper uses a machine learning approach to study the structural stability and evolution of Amycolatopsis eburnea lipases. By analyzing the solvent-accessible surface area (SASA) and conserved structural features, the lipases are clustered into three groups, revealing the importance of certain residues in enzyme stability.
Article
Biotechnology & Applied Microbiology
Neerja Yadav, Lata Nain, Sunil Kumar Khare
Summary: Polylactic acid (PLA) is a key polymer due to its ecofriendly, biocompatible, and biodegradable properties, with potential to replace conventional polymers in various applications. Efforts to accelerate PLA degradation aim to enhance its commercial appeal, while the development of a bioprocess for high molecular weight PLA using enzymatic polymerization technique shows promise for future applications.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Food Science & Technology
Xiaosan Wang, Yu Zhao, Cong Jiang, Ming Chang, Jianhua Huang, Dan Xie
Summary: Bornyl linoleate was synthesized by enzymatic esterification of borneol with linoleic acid in a solvent-free system. The optimal conditions included 8% AYS lipase as catalyst, a reaction temperature of 45 degrees C, a molar ratio of borneol to linoleic acid of 1:2, and a 12-hour reaction time, resulting in a yield of 92.62% and a purity of 99.02%. The final product was identified through mass spectrometry and nuclear magnetic resonance as bornyl linoleate.
News Item
Optics
Enrico Allaria, Giovanni De Ninno
Summary: The demonstration of a low-loss diamond mirror cavity that can temporally store X-ray pulses brings hope for a future generation of X-ray free electron lasers.
Article
Biochemistry & Molecular Biology
Daniela Remonatto, Nubia Santaella, Lindomar Alberto Lerin, Juliana Cristina Bassan, Marcel Otavio Cerri, Ariela Veloso de Paula
Summary: This study aimed to synthesize medium-long-medium (MLM) triacylglycerols with nutraceutical applications through enzymatic acidolysis in a fluidized bed reactor. The optimal conditions were found to be a 1:4 oil/acid molar ratio and 80 cycles, resulting in a degree of incorporation of about 40 mol%.
Article
Chemistry, Medicinal
Marzena Wyskocka-Gajda, Lukasz Przypis, Monika Olesiejuk, Tomasz Krawczyk, Anna Kuznik, Krzysztof Nawara, Masafumi Minoshima, Fuminori Sugihara, Kazuya Kikuchi, Nikodem Kuznik
Summary: Investigation into iron(III) chelates with N,O-aminophenol showed high versatility and tuning potential, with functionalization with galactose providing a bioresponsive character sensitive to enzyme activity. The resulting complexes exhibited extremely high relaxivity and could change relaxivity by over 80% through enzymatic hydrolysis. MRI and cytotoxicity studies confirmed the bioresponsive character and good biocompatibility of these iron-based chelates.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Plant Sciences
Qamar Uz Zaman, Muhammad Rashid, Rab Nawaz, Afzal Hussain, Kamran Ashraf, Maria Latif, Abdihakim Osman Heile, Faisal Mehmood, Sughra Salahuddin, Yinglong Chen
Summary: The study found that foliar applications of silicate chemicals can enhance leaf gas exchange attributes and yield traits, while reducing cadmium accumulation in rice organs.
Article
Energy & Fuels
Francesco Pantano, Flavia Tramontana, Michele Iuliani, Giulia Leanza, Sonia Simonetti, Alessandra Piccoli, Annalisa Paviglianiti, Alessio Cortellini, Gian Paolo Spinelli, Umile Giuseppe Longo, Rocky Strollo, Bruno Vincenzi, Giuseppe Tonini, Nicola Napoli, Daniele Santini
Summary: Direct esterification between glycerol and carboxylic acids allows for the design of glycerides for different applications. Immobilized lipase B from Candida antarctica demonstrated higher selectivity and yield at lower temperatures. The decrease in solubility between carboxylic acid and glycerol favored the formation of monoglycerides. The reaction rate decreased with increasing molecular weight of the carboxylic acid and the introduction of unsaturation.
Article
Chemistry, Applied
Siyu Zhang, Joseph R. Hyatt, Casimir C. Akoh
Summary: A novel diacylglycerol-based galloyl structured lipid, 1,2-dipalmitoylgalloylglycerol (DPGG), was synthesized through enzymatic transesterification process, and the structure of the reaction product was elucidated. The reaction conditions affecting the yield of DPGG were optimized in the study.
Article
Materials Science, Multidisciplinary
Abderrahime Sekkat, Houssein El Housseiny, Eleni Poupaki, Benjamin Breig, Diane Samelor, Jerome Esvan, Olivier Marsan, Cedric Charvillat, Alessandro Pugliara, Nicolas Causse, Hugues Vergnes, Marc Ternisien, Cedric Renaud, Georges Zissis, Brigitte Caussat
Summary: Organic light-emitting diodes (OLEDs) are emerging technologies for lighting and display applications. Researchers have investigated the use of poly(3,4-ethylenedioxythiophene) (PEDOT) thin films as a potential substitute for indium tin oxide (ITO) in OLEDs. The deposition of PEDOT thin films using a single-step dry method demonstrated improved performance compared to ITO.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Engineering, Biomedical
Peter A. Johnson, Sara Menegatti, Adam C. Chambers, Dominic Alibhai, Tracey J. Collard, Ann C. Williams, Hagan Bayley, Adam W. Perriman
Summary: This study describes the development of a high-throughput bioprinted colorectal cancer (CRC) spheroid platform that is highly automated and provides rich information content, with low cell number requirement. By using a hydrogel bioink with a compressive Young's modulus similar to that of colonic tissue, spheroids from various CRC cell lines can grow exponentially. The study also found that bioprinted CRC spheroids exhibit higher levels of chemoresistance compared to cell monolayers.
Review
Chemistry, Multidisciplinary
Ning Gao, Stephen Mann
Summary: Complex coacervate microdroplets derived from associative phase separation have shown great potential in constructing membraneless protocells. However, the lack of an enclosing membrane limits their functionality. In this Account, we present chemical strategies for the membranization of coacervate microdroplets and discuss their potential applications as functional materials. We hope that this Account will inspire further research and development in this field.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Charles de Kergariou, Frederic Demoly, Adam Perriman, Antoine Le Duigou, Fabrizio Scarpa
Summary: 4D printing technology enables the rapid development of multifunctional structures by combining smart materials, stimuli, and additive manufacturing. Hygromorphs, a type of smart material, exhibit properties and morphing capabilities that are dependent on humidity. However, a comprehensive design process for producing hygromorphs using multiple 4D printing techniques is currently unavailable.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Qi-Zhi Zhong, Joseph J. J. Richardson, Yuan Tian, Haijiang Tian, Jiwei Cui, Stephen Mann, Frank Caruso
Summary: Metal-quinone networks (MQNs) have been developed as structurally tunable, universally adhesive, hydrophilic, and pH-degradable materials by coordinating metal ions with quinones through metal-acetylacetone coordination bonds. MQNs exhibit bidirectional pH-responsive disassembly and can be assembled into various forms such as particles, tubes, capsules, and films. Leveraging the tunable release ability of MQNs and the medicinal properties of quinones, MQN prodrugs with high drug loading are engineered for anti-cancer therapy and inhibition of the main protease in the SARS-CoV-2 virus.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biophysics
Jennifer C. Brookes, Eleanor R. Gray, Colleen N. Loynachan, Michelle J. Gut, Benjamin S. Miller, Alex P. S. Brogan, Rachel A. McKendry
Summary: Protein-protein interactions are crucial for life processes. This study used qualitative and quantitative experimental and computational approaches to investigate the contribution of entropy and enthalpy in nanobodies binding to HIV-1 capsid protein p24. The results showed that differences in entropy between amino acids and water molecules can cause orders of magnitude differences in affinity.
BIOPHYSICAL JOURNAL
(2023)
Article
Materials Science, Multidisciplinary
Charles de Kergariou, Antoine Le Duigou, Adam Perriman, Fabrizio Scarpa
Summary: This work explores the design space of bio-based and humidity-triggered 4D printed shape-changing biocomposites through fabrication, modelling, and testing. The aim is to enhance the understanding of control actuation through printing path tailoring and unlock new applications for biomaterials and autonomous actuator design. The composites, made of flax yarns and polylactic acid matrix filaments, demonstrate moisture-induced actuation. Different composite architectures, including cross-ply configurations, are developed to achieve the desired actuation curvature. A finite element model is used to capture the complexity of the printed hygromorphs.
MATERIALS & DESIGN
(2023)
Article
Chemistry, Multidisciplinary
Omar Rifaie-Graham, Jonathan Yeow, Adrian Najer, Richard Wang, Rujie Sun, Kun Zhou, Tristan N. Dell, Christopher Adrianus, Chalaisorn Thanapongpibul, Mohamed Chami, Stephen Mann, Javier Read de Alaniz, Molly M. Stevens
Summary: This study describes a non-equilibrium nanosystem that utilizes light-gated chemical communication, controllable feedback, and macroscopic oscillations. The system allows for communication between nanoreactors and induces chemical mechanical oscillations in a hydrogel using feedback loops.
Article
Chemistry, Multidisciplinary
Ali Heidari, Oya I. I. Sentuerk, Shuo Yang, Alex Joesaar, Pierangelo Gobbo, Stephen Mann, Tom F. A. de Greef, Seraphine V. V. Wegner
Summary: The authors developed a method to control the communication between sender-receiver pairs in a synthetic cell community through red and blue light illumination. They used photoswitchable membrane adhesion to self-assemble sender and receiver cells into different multicellular structures under red and blue light, allowing specific communication without interference. This work demonstrates the potential for designing orthogonal chemical communication networks in diverse synthetic cell communities.
Article
Chemistry, Multidisciplinary
Hannah Taylor, Ning Gao, Stephen Mann
Summary: We fabricate Janus-like calcium alginate hydrogel microspheres with spatially segregated populations using microfluidics. These microspheres serve as integrated platforms for domain-mediated chemical communication and programmable protocell-matrix dynamics. Our results offer new possibilities for the design and construction of spatially organized cytomimetic consortia capable of endogenous chemical processing and protocell-environment interactivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Thomas R. Harrison, Vijai Kumar Gupta, Parvez Alam, Adam Willis Perriman, Fabrizio Scarpa, Vijay Kumar Thakur
Summary: The recognition of the harm caused by conventional and fossil-based materials, particularly single-use and non-biodegradable plastics, is increasing. Therefore, addressing climate change and plastic waste pollution requires a more responsible approach to sourcing raw materials and using less destructive end-of-life pathways. This review assesses recent studies on manure-based cellulose materials, examining their material characteristics and implications for sustainability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Yanwen Zhang, Zefeng Wang, Mei Li, Can Xu, Ning Gao, Zhuping Yin, Kemin Wang, Stephen Mann, Jianbo Liu
Summary: The design and construction of synthetic protocells capable of stimuli response and homeostatic regulation is an important challenge for synthetic protobiology. In this study, we demonstrate a reconfigurable model protocell based on osmotic reconfiguration of lipid-coated coacervate droplets into multicompartmentalized coacervate vesicles that can exhibit hypotonic stress-induced volume response and enhance enzyme reactions. The increased nitric oxide (NO) within the swollen vesicles can induce blood vessel vasodilation, showing potential applications in biomedicine, cellular diagnostics, and bioengineering.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Multidisciplinary
Chengying Yin, Xingyu Jiang, Stephen Mann, Liangfei Tian, Bruce W. Drinkwater
Summary: The high throughput deposition of microscale objects with precise spatial arrangement is crucial in microfabrication technology. The utilization of dynamic acoustic fields offers a contactless approach to real-time reconfigurable miniaturized systems, with potential applications in various fields. This article discusses the physical interactions of microscale objects in an acoustic pressure field, the fabrication of acoustic trapping devices, methods to tune the spatial arrangement, and potential applications in different disciplines.
Article
Chemistry, Multidisciplinary
Patrick J. Grimes, Mary Jenkinson-Finch, Henry E. Symons, Wuge H. Briscoe, Sebastien Rochat, Stephen Mann, Pierangelo Gobbo
Summary: The synthesis of a novel photocleavable, poly(ethylene) glycol-based crosslinker enables the realization of light-induced disassembly, pattern generation and membrane permeability modulation in protocells, opening up new avenues for the engineering of organized protocell networks and micro-compartmentalized systems for information storage and release.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Zhuping Yin, Ning Gao, Can Xu, Mei Li, Stephen Mann
Summary: This article describes a self-driven, endosymbiotic-like pathway where proteinosomes are captured within membranized alginate/silk fibroin coacervate vesicles by guest-mediated reconfiguration. The interchange of coacervate vesicle and droplet morphologies through proteinosome-mediated activity produces discrete nested communities with integrated catalytic activity and selective disintegration.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Materials Science, Biomaterials
Naomi Akiyama, Kapil D. Patel, Eun Jo Jang, Mark R. Shannon, Rajkumar Patel, Madhumita Patel, Adam Willis Perriman
Summary: The composition, morphology, and mechanical performance of nanomaterials are crucial for tissue engineering. Tubular nanomaterials (TNs), including carbon nanotubes, titanium oxide nanotubes, halloysite nanotubes, silica nanotubes, and hydroxyapatite nanotubes, have shown great potential in various applications due to their high surface area, versatile surface chemistry, well-defined mechanical properties, excellent biocompatibility, and monodispersity. This review focuses on the recent developments in TN-based biomaterials for structural tissue engineering, with a strong emphasis on bone tissue regeneration. It includes a detailed literature review on TN-based orthopedic coatings for metallic implants and composite scaffolds to enhance in vivo bone regeneration.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)