Article
Biochemistry & Molecular Biology
Natalia Nosalova, Alexandra Keselakova, Martin Kello, Miroslava Martinkova, Dominika Fabianova, Martina Bago Pilatova
Summary: Despite the decreasing trend in mortality from colorectal cancer, this study focused on the antiproliferative and pro-apoptotic effects of a compound on colon cancer cells. The compound was found to have concentration-dependent cytotoxic and antiproliferative effects, inducing both intrinsic and extrinsic apoptotic pathways and inhibiting cell migration. These findings suggest the potential of this compound as a future treatment for colon cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Ali Rafat, Khadijeh Dizaji Asl, Zeinab Mazloumi, Ali Akbar Movassaghpour, Mehdi Talebi, Dariush Shanehbandi, Raheleh Farahzadi, Babak Nejati, Hojjatollah Nozad Charoudeh
Summary: The study revealed that Telomerase Inhibition (TI) induced apoptosis through intrinsic, extrinsic, and p53 pathways and increased the expression of DNMT3a and TET2 epigenetic markers.
Article
Biology
Po-Fu Yueh, Yuan-Hao Lee, Chun-Yu Fu, Chun-Bin Tung, Fei-Ting Hsu, Keng-Li Lan
Summary: The study found that magnolol can inhibit proliferation and induce apoptosis in GBM cells, as well as suppress their invasion and migration abilities. The anti-GBM effect of magnolol may be associated with the inactivation of the PKC delta/STAT3 signaling pathway.
Article
Environmental Sciences
Chun-Hung Su, Shih-Pin Chen, Li-You Chen, Jiann-Jou Yang, Yi-Chia Lee, Shiuan-Shinn Lee, Hsin-Hung Chen, Yan-Yan Ng, Yu-Hsiang Kuan
Summary: 3-BrFlu induces cardio- and embryotoxicity in zebrafish through cytotoxicity in vascular endothelial cells, triggering apoptosis via caspase-dependent intrinsic and extrinsic pathways.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Jianye Wang, Simin Lu, Kang Zheng, Zhangping He, Weiwei Li, Jie Liu, Ningyuan Guo, Yafeng Xie, Dejun Chen, Man Xu, Yimou Wu
Summary: It has been discovered that Treponema pallidum can be phagocytosed by human polymorphonuclear neutrophils (hPMNs) in vitro, and it can significantly inhibit the apoptosis of these neutrophils. This effect is not influenced by bacterial viability and might be achieved through the production of the anti-apoptotic cytokine IL-8.
MOLECULAR IMMUNOLOGY
(2022)
Article
Oncology
Ahmed A. Abd-Rabou, Hanaa H. Ahmed, Mohamed S. Kishta
Summary: In this study, aptamer nanoparticles loaded with viramidine (VRM) were used to interfere with hepatocellular carcinoma (HCC) cells through NO-mediated extrinsic and intrinsic apoptosis.
Article
Food Science & Technology
Pei-Yu Chou, Ya-Ting Lu, Ming-Jyh Sheu
Summary: This study found that ethanol extract of Phellinus merrillii (EPM) can attenuate balloon injury-induced neointima formation by inducing apoptosis of vascular smooth muscle cells (VSMC). Western blotting analysis showed that EPM can induce VSMC apoptosis through intrinsic and extrinsic pathways.
FOOD SCIENCE & NUTRITION
(2023)
Article
Environmental Sciences
Wenna Liu, Yujin Gan, Yun Ding, Lina Zhang, Xiaojing Jiao, Lu Liu, Huixia Cao, Yue Gu, Lei Yan, Yanliang Wang, Limeng Wang, Song Chen, Fengmin Shao
Summary: This study reveals the involvement of GSDME-mediated pyroptosis in cobalt-induced acute kidney injury (AKI), furthering the understanding of the regulatory mechanism of GSDME cleavage.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Multidisciplinary Sciences
Nehal M. El-Deeb, Sara M. Khattab, Morsy A. Abu-Youssef, Ahmed M. A. Badr
Summary: In this study, gold nanoparticles (AuNPs) were synthesized using algal polysaccharides and their properties and applications were analyzed. The results showed that AuNPs exhibited significant antimicrobial effects against E. coli and demonstrated high safety in noncancerous cells. Furthermore, AuNPs showed promising anticancer activity against MCF-7 cells, inhibiting cell growth through gene expression regulation and cell cycle arrest.
SCIENTIFIC REPORTS
(2022)
Review
Hematology
Emma C. Josefsson
Summary: This review focuses on the molecular regulation of intrinsic apoptosis in platelets, conditions associated with enhanced platelet death, ex vivo storage of platelets, and caveats related to the assessment of platelet apoptosis.
THROMBOSIS RESEARCH
(2023)
Article
Cell Biology
Chung-Yuan Lee, Yi-Hsuan Hsiao, Pei-Ni Chen, Heng-Hsiung Wu, Chih-Yun Lu, Shun-Fa Yang, Po-Hui Wang
Summary: This study investigated the effects and mechanism of action of CLEFMA, a synthetic derivative of curcumin, on cervical cancer cells. The results showed that CLEFMA negatively regulated cell viability and induced apoptosis in cervical cancer cells. Treatment with CLEFMA increased the expression of cleaved caspase-3, cleaved poly(adenosine diphosphate-ribose) polymerase, cleaved caspase-8, and cleaved caspase-9. Co-treatment with inhibitors of ERK1/2 and p38 significantly reduced the expression of cleaved caspase-8, -9, and -3. In conclusion, CLEFMA activates both extrinsic and intrinsic apoptotic pathways through ERK1/2 and p38 signal transduction in cervical cancer cells.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Goutham Venkata Naga Davuluri, Chia-Hsin Chan
Summary: TAMs can be converted between different functional states through the regulation of cytokines and metabolites in the microenvironment. Dysregulated metabolism plays a crucial role in TAM differentiation and function. Extracellular metabolites within the tumor microenvironment can regulate TAMs through competition for metabolite availability and direct signaling reactions.
Article
Plant Sciences
Xiudong Yang, Zi-Fan Bai, Yan Zhang, Hao Cui, Hong-Li Zhou
Summary: The study demonstrated that flavonoids-rich extract from Bidens bipinnata exhibited protective effects on H2O2-induced pancreatic beta cell damage by influencing the expression of apoptosis-related proteins and regulating cell signaling pathways.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Immunology
Hsin-Hung Lin, Hsiu-Ling Chen, Chang-Ching Weng, Rajendra Prasad Janapatla, Chyi-Liang Chen, Cheng-Hsun Chiu
Summary: The study revealed that Salmonella induces apoptosis in macrophages through caspase-3 activation, especially via the expression of SPI-1 structure proteins and effectors. This mechanism may help explain why Salmonella infections in children often present as mild bacteremia with less systemic inflammatory response.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Oncology
Hussain Mubarak Al-Aamri, Helen R. Irving, Christopher Bradley, Terri Meehan-Andrews
Summary: Daunorubicin induces apoptosis in leukemic cells through both extrinsic and intrinsic pathways, with variations in sensitivities and timing of apoptotic responses observed among different cell lines. The lack of functional p53 may contribute to these differences and necessitates further exploration of alternative signaling mechanisms involved in apoptosis induction.
Article
Biochemistry & Molecular Biology
Stefanie M. Bader, Simon P. Preston, Katie Saliba, Adam Lipszyc, Zoe L. Grant, Liana Mackiewicz, Andrew Baldi, Anne Hempel, Michelle P. Clark, Thanushi Peiris, William Clow, Jan Bjelic, Michael D. Stutz, Philip Arandjelovic, Jack Teale, Fashuo Du, Leigh Coultas, James M. Murphy, Cody C. Allison, Marc Pellegrini, Andre L. Samson
Summary: Caspase-8 mediates various responses including inflammation, cell proliferation, and cell death. Its function during embryogenesis is essential for maintaining systemic circulatory system, while in adulthood it is crucial for preserving gut vascular integrity. Lack of endothelial Caspase-8 leads to fatal hemorrhagic lesions in the small intestine, resulting from aberrant microbial sensing and tumor necrosis factor receptor signaling.
CELL DEATH AND DIFFERENTIATION
(2023)
Review
Medicine, General & Internal
Jessica L. Fairley, Mandana Nikpour, Heather G. Mack, Maria Brosnan, Amanda M. Saracino, Marc Pellegrini, Ian P. Wicks
Summary: Hydroxychloroquine (HCQ) and chloroquine (CQ) were originally used as antimalarial drugs but are now widely used in rheumatology, dermatology, and immunology for the treatment of autoimmune diseases. HCQ plays a key role in the management of connective tissue diseases and inflammatory arthritis, and is considered to have a better long-term safety profile compared to CQ. The controversy surrounding the toxicity of HCQ in COVID-19 has led to discussions about its potential adverse effects.
INTERNAL MEDICINE JOURNAL
(2023)
Article
Hematology
Amanda E. Au, Jason Corbin, Marion Lebois, Pradnya Gangatirkar, Fatme Yassinson, Stephanie R. Hyslop, Ping Cannon, Kylie D. Mason, Connie S. N. Li-Wai-Suen, Alexandra L. Garnham, Diane Moujalled, Luisa Cimmino, Warren S. Alexander, Emma C. Josefsson
Article
Biochemistry & Molecular Biology
Zilu Wang, Huimin Hu, Luuk Heitink, Kelly Rogers, Yue You, Tao Tan, Connie Li Wai Suen, Alex Garnham, Hao Chen, Elizabeth Lieschke, Sarah T. T. Diepstraten, Catherine Chang, Diane Moujalled, Kate Sutherland, Guillaume Lessene, Oliver M. M. Sieber, Jane Visvader, Gemma L. L. Kelly, Andreas Strasser
Summary: APR-246 can kill malignant cells irrespective of their TP53/TRP53 status. It induces various programmed cell death pathways, such as apoptosis, necroptosis, and ferroptosis. Therefore, the clinical testing of APR-246 should be expanded to cancers expressing wild-type TP53 or TP53-deficient.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Sebastian A. Hughes, Meng Lin, Ashley Weir, Bing Huang, Liya Xiong, Ngee Kiat Chua, Jiyi Pang, Jascinta P. Santavanond, Rochelle Tixeira, Marcel Doerflinger, Yexuan Deng, Chien-Hsiung Yu, Natasha Silke, Stephanie A. Conos, Daniel Frank, Daniel S. Simpson, James M. Murphy, Kate E. Lawlor, Jaclyn S. Pearson, John Silke, Marc Pellegrini, Marco Herold, Ivan K. H. Poon, Seth L. Masters, Mingsong Li, Qin Tang, Yuxia Zhang, Maryam Rashidi, Lanlan Geng, James E. Vince
Summary: This study reveals that genetic mutations in X-linked inhibitor of apoptosis (XIAP) can lead to cell death-associated inflammatory diseases. The findings show that patients with XIAP deficiency-associated inflammatory bowel disease have increased inflammatory IL-1 beta maturation and cell death-associated caspase-8 and Gasdermin D (GSDMD) processing, which can be reduced by patient treatment.
Review
Hematology
Emma C. Josefsson
Summary: This review focuses on the molecular regulation of intrinsic apoptosis in platelets, conditions associated with enhanced platelet death, ex vivo storage of platelets, and caveats related to the assessment of platelet apoptosis.
THROMBOSIS RESEARCH
(2023)
Article
Cell Biology
Simon P. Preston, Cody C. Allison, Jan Schaefer, William Clow, Stefanie M. Bader, Sophie Collard, Wasan O. Forsyth, Michelle P. Clark, Alexandra L. Garnham, Connie S. N. Li-Wai-Suen, Thanushi Peiris, Jack Teale, Liana Mackiewicz, Sophia Davidson, Marcel Doerflinger, Marc Pellegrini
Summary: This study investigated the role of RIPK3 and MLKL in chronic LCMV infection and found that during the acute and early stages of infection, MLKL and RIPK3 did not affect the immune response and viral clearance. However, RIPK3 was found to promote immune dysfunction and prevent control of infection at later stages of chronic LCMV disease.
CELL DEATH & DISEASE
(2023)
Article
Medicine, General & Internal
Georgia Deliyannis, Nicholas A. Gherardin, Chinn Yi Wong, Samantha L. Grimley, James P. Cooney, Samuel J. Redmond, Paula Ellenberg, Kathryn C. Davidson, Francesca L. Mordant, Tim Smith, Marianne Gillard, Ester Lopez, Julie McAuley, Chee Wah Tan, Jing J. Wang, Weiguang Zeng, Mason Littlejohn, Runhong Zhou, Jasper Fuk-Woo Chan, Zhi-wei Chen, Airn E. Hartwig, Richard Bowen, Jason M. Mackenzie, Elizabeth Vincan, Joseph Torresi, Katherine Kedzierska, Colin W. Pouton, Tom P. Gordon, Lin-fa Wang, Stephen J. Kent, Adam K. Wheatley, Sharon R. Lewin, Kanta Subbarao, Amy W. Chung, Marc Pellegrini, Trent Munro, Terry Nolan, Steven Rockman, David C. Jackson, Damian F. J. Purcell, Dale I. Godfrey
Summary: Researchers have developed a protein subunit vaccine to address the variants of the SARS-CoV-2 virus. The vaccine induces strong neutralizing antibody responses and provides durable immunity against upper and lower airway infections. It has the potential to complement existing vaccines and is currently in a phase I clinical trial.
Article
Multidisciplinary Sciences
Tomalika R. Ullah, Matt D. Johansen, Katherine R. Balka, Rebecca L. Ambrose, Linden J. Gearing, James Roest, Julian P. Vivian, Sunil Sapkota, W. Samantha N. Jayasekara, Daniel S. Wenholz, Vina R. Aldilla, Jun Zeng, Stefan Miemczyk, Duc H. Nguyen, Nicole G. Hansbro, Rajan Venkatraman, Jung Hee Kang, Ee Shan Pang, Belinda J. Thomas, Arwaf S. Alharbi, Refaya Rezwan, Meredith O'Keeffe, William A. Donald, Julia I. Ellyard, Wilson Wong, Naresh Kumar, Benjamin T. Kile, Carola G. Vinuesa, Graham E. Kelly, Olivier F. Laczka, Philip M. Hansbro, Dominic De Nardo, Michael P. Gantier
Summary: The small molecule idronoxil inhibits the signalling of TBK1/IKK epsilon, a key signalling component involved in antiviral activities and pro-inflammatory responses. This finding highlights the critical role of TBK1/IKK epsilon signalling in SARS-CoV-2 hyper-inflammation.
NATURE COMMUNICATIONS
(2023)
Review
Infectious Diseases
Stephen Muhi, Joshua Osowicki, Daniel O'Brien, Paul D. R. Johnson, Sacha Pidot, Marcel Doerflinger, Julia L. L. Marshall, Marc Pellegrini, James McCarthy, Timothy P. P. Stinear
Summary: Critical knowledge gaps on Mycobacterium ulcerans infection have hindered development of new therapies and vaccines for Buruli ulcer. We review host-pathogen interactions and immune protection and propose a controlled human infection model. We also discuss safety considerations and strain selection.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Cell Biology
Philip Arandjelovic, Youry Kim, James P. Cooney, Simon P. Preston, Marcel Doerflinger, James H. Mcmahon, Sarah E. Garner, Jennifer M. Zerbato, Michael Roche, Carolin Tumpach, Jesslyn Ong, Dylan Sheerin, Gordon K. Smyth, Jenny L. Anderson, Cody C. Allison, Sharon R. Lewin, Marc Pellegrini
Summary: This study suggests that inhibiting host pro-survival proteins may help eliminate HIV-1 infected cells that persist during ART treatment, delaying viral rebound.
CELL REPORTS MEDICINE
(2023)
Article
Hematology
Emma C. Josefsson, Sofia Ramstrom, Johannes Thaler, Marie Lordkipanidze, COAGAPO Study Grp
Summary: The study aimed to identify a set of markers and/or methods to detect and distinguish procoagulant platelets from apoptotic platelets. Using flow cytometry, a combination of three surface markers, including P-selectin (CD62P), phosphatidylserine (recognized by annexin V), and platelet-specific receptor GPIX (CD42a) or αIIb integrin (CD41, GPIIb), was recommended for differentiation. Procoagulant platelets are positive for all three markers, while apoptotic platelets are positive for annexin V and platelet-specific surface receptor(s) but negative for P-selectin.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)