Article
Cell Biology
Anouar Hafiane, Ioanna Gianopoulos, Mary G. Sorci-Thomas, Stella S. Daskalopoulou
Summary: The study found that adiponectin plays a protective role in cholesterol efflux, but the involvement of apoA-I residues in this process has not been studied. This study used macrophages and cell models to show that adiponectin requires specific residues of apoA-I and wild-type ABCA1 to restore cholesterol efflux. Adiponectin cannot rescue defective cholesterol efflux in apoA-I or ABCA1 mutant conditions, but it increases cholesterol efflux compared to apoA-I exposure alone in wild-type apoA-I conditions.
CELLULAR SIGNALLING
(2022)
Article
Multidisciplinary Sciences
Sachiko Fujiwara, Thanh Phuong Nguyen, Kyoko Furuse, Yugo Fukazawa, Tetsuhisa Otani, Mikio Furuse
Summary: The interaction of claudins with ZO proteins is not a prerequisite for tight junction formation at the most apical part of cell-cell contacts.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Ji Hwan Kim, Jonghu Jeon, Young Lee, Seung Min Kim, Miju Cheon, Jun Yup Kim
Summary: This study aimed to understand the development of dysphagia in patients with Parkinson's disease (PD) and identify potential target regions for neuromodulation by analyzing the distribution of dopamine transporter availability in the striatum. The results showed that decreased dopamine transporter availability in specific subregions of the striatum may explain the dysphagia in PD, particularly in the pharyngeal phase.
CLINICAL NUCLEAR MEDICINE
(2023)
Article
Medicine, General & Internal
Chao Wang, Cheng Zhou, Tao Guo, Yeerfan Jiaerken, Siyu Yang, Xiaopei Xu, Ling Hu, Peiyu Huang, Xiaojun Xu, Minming Zhang
Summary: This study found that current coffee consumption is associated with decreased striatal dopamine transporter availability in the caudate. However, the effects of caffeine on dopamine transporter may fade and disappear after quitting coffee consumption.
Article
Clinical Neurology
Han Soo Yoo, Seong Ho Jeong, Kyeong Taek Oh, Sangwon Lee, Young H. Sohn, Byoung Seok Ye, Mijin Yun, Phil Hyu Lee
Summary: Yoo et al. investigated the relationship between striatal dopamine depletion, brain metabolism, and cognition in patients with dementia with Lewy bodies. They found that striatal dopamine loss and brain hypometabolism are closely related, impacting cognition in a specific manner, and predicting cognitive decline. Brain hypometabolism was found to be more relevant to longitudinal cognitive outcomes than striatal dopaminergic degeneration in patients with Lewy body dementia.
Article
Biochemistry & Molecular Biology
Boglarka Zambo, Gergo Gogl, Bastien Morlet, Pascal Eberling, Luc Negroni, Herve Moine, Gilles Trave
Summary: This study comprehensively investigates the PDZ-PBM interactome of diacylglycerol kinases (DGKs) using interactomic approaches and evolutionary analysis, revealing functional differences between PBMs of different types of DGKs.
Article
Psychiatry
Ivan Milenkovic, Lucie Bartova, Konstantinos Papageorgiou, Siegfried Kasper, Tatjana Traub-Weidinger, Dietmar Winkler
Summary: Diagnosing parkinsonian syndromes in patients with severe depression can be challenging due to overlapping clinical phenomena. [I-123]FP-CIT-SPECT is a useful method for detecting degenerative parkinsonian disorders but drugs may influence the results. Consultation with a movement disorder specialist prior to dopamine transporter imaging is recommended.
FRONTIERS IN PSYCHIATRY
(2021)
Article
Neurosciences
Melissa M. Conti Mazza, Ashley Centner, David F. Werner, Christopher Bishop
Summary: L-DOPA is the standard treatment for Parkinson's disease, but chronic treatment leads to L-DOPA-induced dyskinesia. This study found that dopamine depletion and L-DOPA treatment alter the expression and function of dopamine and serotonin transporters, suggesting their potential as biomarkers and therapeutic targets for dyskinesia in Parkinson's disease patients.
Article
Biochemistry & Molecular Biology
Emilie Aponte, Marie Lafitte, Audrey Sirvent, Valerie Simon, Maud Barbery, Elise Fourgous, Mariano Maffei, Florence Armand, Romain Hamelin, Julie Pannequin, Philippe Fort, Miquel Pons, Serge Roche, Yvan Boublik
Summary: This study reveals the important role of the unique domain ULBR in Src tyrosine kinase in malignant cell transformation. The ULBR is involved in membrane anchoring, MAPK signaling, and phosphorylation of specific membrane-localized tyrosine kinases needed for Src oncogenic signaling.
Article
Neurosciences
L. Duart-Castells, N. Nadal-Gratacos, M. Muralter, B. Puster, X. Berzosa, R. Estrada-Tejedor, M. Niello, S. Bhat, D. Pubill, J. Camarasa, H. H. Sitte, E. Escubedo, R. Lopez-Arnau
Summary: The study highlights the role of amino terminal substitution in the pharmacological profile of novel synthetic cathinones, including their potency in inhibiting dopamine uptake and inducing psychostimulant and rewarding effects in mice. The results demonstrate a significant correlation between predicted binding affinities by molecular docking and affinity constants for human dopamine transporter, indicating the importance of amino substitutions in determining the biological activity of these compounds.
Article
Multidisciplinary Sciences
Ailen S. Cervino, Mariano G. Collodel, Ivan A. Lopez, Carolina Roa, Daniel Hochbaum, Neil A. Hukriede, M. Cecilia Cirio
Summary: The nephron is the functional unit of the vertebrate kidney, and studying the Xenopus laevis pronephric kidney provides a simplified model for nephrogenesis. This study identified the interaction between single-stranded DNA binding protein 2 (Ssbp2) and the Ldb1-Lhx1 complex, which plays a crucial role in kidney organogenesis.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Michelle America, Naguissa Bostaille, Marie Eubelen, Maud Martin, Didier Y. R. Stainier, Benoit Vanhollebeke
Summary: This study reveals the molecular mechanisms underlying the interactions between Gpr124 and Frizzled in zebrafish and mammals, and provides insights into the evolution of Gpr124/Reck function in vertebrates.
Article
Biochemistry & Molecular Biology
Elek Telek, Kristof Karadi, Jozsef Kardos, Andras Kengyel, Zsuzsanna Fekete, Henriett Halasz, Miklos Nyitrai, Beata Bugyi, Andras Lukacs
Summary: The Myo16Tail protein region plays a crucial role in neuronal functioning, cell cycle regulation, and phosphoinositide 3-kinase signaling. It interacts with the N-terminal ankyrin domain of myosin 16, demonstrating high structural flexibility and intrinsic disorder. These features suggest that Myo16Tail may act as a flexible display site crucial in post-translational modifications and regulatory functions.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Louise Laursen, Raviteja Inturi, Soren Ostergaard, Per Jemth
Summary: This study investigated the interaction between the PSG supramodule and other proteins in the post-synaptic density using bioinformatics and biophysical methods. The results showed that the PDZ3 domain had specific binding with peptides, and mutations in PDZ3 could affect the affinity and specificity of the interaction. Additionally, the study found that the ADGRB1 protein formed liquid droplets with the PSG supramodule, providing new insights into PSD formation.
Article
Neurosciences
Riccardo Viaro, Francesco Longo, Fabrizio Vincenzi, Katia Varani, Michele Morari
Summary: Previous studies have demonstrated that L-DOPA can enhance dopamine release through SCH23390-sensitive D1 receptors and potentially promote dopamine efflux via conversion to dopamine and reversal of the DA transporter.
Article
Clinical Neurology
Marco Moller, Simon Fredholm, Mathias E. Jensen, Gitta Wortwein, Julie R. Larsen, Tina Vilsboll, Niels Odum, Anders Fink-Jensen
Summary: The study aimed to evaluate the relationship between the therapeutic effects of glucagon-like peptide-1 receptor agonist liraglutide and cytokines. The results showed that prediabetic patients treated with clozapine or olanzapine had increased levels of inflammatory cytokines in their serum, further confirming the link between inflammation and type 2 diabetes. However, treatment with liraglutide did not affect these cytokines.
Review
Pharmacology & Pharmacy
Mette Kruse Klausen, Morgane Thomsen, Gitta Wortwein, Anders Fink-Jensen
Summary: Drug, alcohol and tobacco use disorders are a global burden. GLP-1 receptor agonists have potential as anti-addiction treatment. Animal and clinical studies have shown a reduction in alcohol and drug abuse with GLP-1 treatment.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Solveig G. Schmidt, Ulrik Gether, Claus J. Loland
Summary: Sodium-coupled neurotransmitter transporters are important in terminating synaptic neurotransmission, and research into their molecular transport mechanisms has been enlightening. Despite their structural differences, studies on neurotransmitter: sodium symporters and excitatory amino acid transporters are crucial in understanding their ion-dependence and transport kinetics. Single-molecule studies have been instrumental in expanding the understanding of protein-level transport across membranes.
NEUROCHEMICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Sigurd Friis Truelsen, Julie Winkel Missel, Kamil Gotfryd, Per Amstrup Pedersen, Pontus Gourdon, Kresten Lindorff-Larsen, Claus Helix-Nielsen
Summary: The study reveals a unique gating mechanism of hAQP10 by simulating three protonation states of H80, showing a specific water coordination pattern at neutral pH and the influence of H80 protonation on the loop conformation and water coordination. The residue F85 plays a stabilizing role in the loop by flipping into the channel during loop movements.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Behavioral Sciences
Anna Molander, Ditte Dencker Thorbek, Christian Lysne, Pia Weikop, Anders Fink -Jensen, Gitta Wortwein
Summary: This study found that mice lacking M4 mAChRs exhibit disinhibited behavior, which is positively correlated with alcohol preference and relapse after abstinence. These data highlight the potential role of M4 mAChRs in improving treatment strategies for alcohol use disorder.
BEHAVIOURAL BRAIN RESEARCH
(2022)
Article
Multidisciplinary Sciences
Aske L. Ejdrup, Matthew D. Lycas, Niels Lorenzen, Ainoa Konomi, Freja Herborg, Kenneth L. Madsen, Ulrik Gether
Summary: This study reports a relative enrichment method for colocalization analysis in both 2D and 3D of single-molecule localization microscopy (SMLM) data. The method uses a function to describe the relative enrichment of one molecular species on the density distribution of a reference species, and visualizes the colocalization directly in images. The application of this method on simulated data and cultured neurons demonstrates its functionality and comparison with current alternative measures.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Anders Wiuf, Jonas Hyld Steffen, Eva Ramos Becares, Christina Gronberg, Dhani Ram Mahato, Soren G. F. Rasmussen, Magnus Andersson, Tristan Croll, Kamil Gotfryd, Pontus Gourdon
Summary: This study investigates the mechanism of zinc transport by ZIP proteins and reveals the structure of metal-free BbZIP. With accompanying experiments, the study suggests an elevator-type transport mechanism and discovers an important ninth transmembrane segment.
Article
Biology
Carmen Klein Herenbrink, Jonatan Fullerton Stoier, William Dalseg Reith, Abeer Dagra, Miguel Alejandro Cuadrado Gregorek, Reto B. Cola, Tommaso Patriarchi, Yulong Li, Lin Tian, Ulrik Gether, Freja Herborg
Summary: This study develops dopamine sensor cell lines expressing fluorescent reporters, which can be used to detect endogenous dopamine release, determine dopamine tissue content, and measure dopamine uptake and efflux. This versatile tool has a wide range of applications.
COMMUNICATIONS BIOLOGY
(2022)
Article
Neurosciences
Mona El-Sayed Hervig, Chiara Toschi, Anders Petersen, Signe Vangkilde, Ulrik Gether, Trevor W. Robbins
Summary: This study investigated the role of dopamine and noradrenaline in attentional processing using the TVA model and the 5-choice serial reaction time task in rats. The results showed that different drugs had varying effects on attention, with some slowing down response speed and impairing visual processing while others speeding up response speed and improving attention. These findings have important implications for understanding and treating attention deficits and psychiatric disorders.
PSYCHOPHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Basavraj Khanppnavar, Julian Maier, Freja Herborg, Ralph Gradisch, Erika Lazzarin, Dino Luethi, Jae-Won Yang, Chao Qi, Marion Holy, Kathrin Jaentsch, Oliver Kudlacek, Klaus Schicker, Thomas Werge, Ulrik Gether, Thomas Stockner, Volodymyr M. Korkhov, Harald H. Sitte
Summary: This study reports the structure of human organic cation transporter 3 (OCT3) and provides insight into its inhibition by two specific inhibitors, decynium-22 and corticosterone. Understanding OCT3 polymorphisms is important, and this research sheds light on their impact.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Carolin Hoefner, Tenna Holgersen Bryde, Celina Pihl, Sylvia Naiga Tiedemann, Sophie Emilie Bresson, Hajira Ahmed Hotiana, Muhammad Saad Khilji, Theodore Dos Santos, Michele Puglia, Paola Pisano, Mariola Majewska, Julia Durzynska, Kristian Klindt, Justyna Klusek, Marcelo J. Perone, Robert Bucki, Per Marten Hagglund, Pontus Emanuel Gourdon, Kamil Gotfryd, Edyta Urbaniak, Malgorzata Borowiak, Michael Wierer, Patrick Edward MacDonald, Thomas Mandrup-Poulsen, Michal Tomasz Marzec
Summary: Apart from known functions, such as chaperoning and disulfide bond formation, the complete molecular sequence of proinsulin folding remains unclear. In this study, we investigated the role of FKBP2, an ER-resident proline isomerase, in proinsulin folding. Through experiments and analysis, we found that FKBP2 depletion resulted in misfolding of proinsulin and increased apoptosis in pancreatic beta-cells. Additionally, we observed an overexpression of FKBP2 mRNA in beta-cells from pancreatic islets of type 2 diabetes patients. These findings suggest that FKBP2 plays a crucial role in the correct folding of proinsulin.
Article
Biochemistry & Molecular Biology
Aske L. Ejdrup, Joel Wellbourne-Wood, Jakob K. Dreyer, Nina Guldhammer, Matthew D. Lycas, Ulrik Gether, Benjamin J. Hall, Gunnar Sorensen
Summary: A fundamental concept in neuroscience is the transmission of information between neurons via neurotransmitters, modulators, and peptides. The gold standard for measuring neurochemicals in awake animals has been microdialysis (MD), but genetically encoded fluorescence-based biosensors and in vivo optical techniques have introduced technologically distinct means of measuring neurotransmission. Comparing MD and fiber photometry (FP), the study found that although there were temporal differences, the readouts of extracellular dopamine (DA) correlated well within mice. Down-sampling of FP data showed temporal correlation to MD, with less variance observed using FP. The study also discovered periodic low levels of DA fluctuations and rapid predrug DA dynamics measured with FP that correlated to the subsequent pharmacodynamics of amphetamine as measured with MD and FP.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Cell Biology
Sarah Falk, Jonas Petersen, Charlotte Svendsen, Cesar R. Romero-Leguizamon, Soren Heide Jorgensen, Nathalie Krauth, Mette Q. Ludwig, Kathrine Lundo, Urmas Roostalu, Grethe Skovbjerg, Duy Anh Gurskov Nielsen, Aske Lykke Ejdrup, Tune H. Pers, Oksana Dmytriyeva, Jacob Hecksher-Sorensen, Ulrik Gether, Kristi A. Kohlmeier, Christoffer Clemmensen
Summary: GLP-1R agonists can promote nicotine avoidance and combined treatment with nicotine and GLP-1R agonist can enhance the anti-obesity effects. GLP-1R agonism increases the excitability of hypothalamic POMC neurons and VTA dopaminergic neurons to suppress nicotine-induced dopamine release, resulting in weight loss. These findings support the use of GLP-1R-based therapies for nicotine dependence and further evaluation of combined treatment.
Article
Medicine, Research & Experimental
Rita C. Andersen, Jan H. Schmidt, Joscha Rombach, Matthew D. Lycas, Nikolaj R. Christensen, Viktor K. Lund, Donnie S. Stapleton, Signe S. Pedersen, Mathias A. Olsen, Mikkel Stoklund, Gith Noes-Holt, Tommas Te Nielsen, Mark P. Keller, Anna M. Jansen, Rasmus Herlo, Massimo Pietropaolo, Jens B. Simonsen, Ole Kjaerulff, Birgitte Holst, Alan D. Attie, Ulrik Gether, Kenneth L. Madsen
Summary: The BAR domain protein PICK1 plays a role in the formation and release of secretory granules, and coding variants associated with diabetes have been identified. The coding variants may cause dominant-negative effects by increasing the ability to induce membrane fission.
JOURNAL OF CLINICAL INVESTIGATION
(2022)