Article
Biochemistry & Molecular Biology
Yang-Hsiang Lin, Meng-Han Wu, Yi-Chung Liu, Ping-Chiang Lyu, Chau-Ting Yeh, Kwang-Huei Lin
Summary: This study identified a novel lncRNA, LINC01348, significantly downregulated in HCC, which inhibited cell growth and metastasis by interacting with splicing factor 3b subunit 3 (SF3B3) to modulate EZH2 pre-mRNA AS. This novel regulatory association involving LINC01348/SF3B3/EZH2/JNK/c-Jun/Snail is a crucial determinant of metastasis in HCC cells, suggesting the potential utility of targeting LINC01348 as a therapeutic strategy for HCC.
Article
Biochemistry & Molecular Biology
Ofri Levi, Yoav S. Arava
Summary: The study identified a novel integrated pathway where Pus6 'writes' modifications on tRNA and mRNA, and both types of RNAs are 'read' by MetRS for translation regulation purposes.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Hung-Chieh Lee, Chuan-Yang Fu, Cheng-Yung Lin, Jia-Rung Hu, Ting-Ying Huang, Kai-Yin Lo, Hsin-Yue Tsai, Jin-Chuan Sheu, Huai-Jen Tsai
Summary: The study reveals that Endouc/ENDOU-1 can enhance CHOP mRNA translation by cleaving the huORF(chop) transcript to release its inhibitory effect on downstream ORF, leading to increased levels of CHOP protein.
Article
Multidisciplinary Sciences
Shiho Makino, Tomoko Kawamata, Shintaro Iwasaki, Yoshinori Ohsumi
Summary: The study demonstrates selective mRNA degradation by autophagy induced by rapamycin in yeast, with certain subsets of mRNAs encoding amino acid biosynthesis and ribosomal proteins being preferentially delivered to vacuoles for degradation. Autophagy-mediated mRNA degradation is closely linked with ribosomal translation, as suggested by genome-wide ribosome profiling, indicating a previously unappreciated role of autophagy in post-transcriptional gene regulation.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Dongming Lv, Shirong Ding, Li Zhong, Jian Tu, Hongbo Li, Hao Yao, Yutong Zou, Ziliang Zeng, Yan Liao, Xuesi Wan, Lili Wen, Xianbiao Xie
Summary: This study discovered that FTO may serve as a novel therapeutic target for osteosarcoma by promoting tumor growth and metastasis through reducing DACT1 expression and activating the Wnt signaling pathway. The research on the drug entacapone confirmed that the m(6)A-mediated FTO/DACT1 axis could be targeted for osteosarcoma treatment.
Article
Cell Biology
Sejeong Shin, Min-Joon Han, Mark P. Jedrychowski, Ziyang Zhang, Kevan M. Shokal, David R. Plas, Noah Dephoure, Sang-Oh Yoon
Summary: Cells change their proteomes according to their environment and needs, and the key regulator of this process is mTOR. Dysregulation of mTOR leads to changes in proteostasis and the progression of diseases, including cancer.
Article
Multidisciplinary Sciences
Larissa Dietz, Cara J. Ellison, Carlos Riechmann, C. Keith Cassidy, F. Daniel Felfoldi, Adan Pinto-Fernandez, Benedikt M. Kessler, Paul R. Elliott
Summary: Certain members of the inhibitor of apoptosis (IAP) family, including BIRC6, regulate cell death by inhibiting caspases, while SMAC antagonizes IAPs and promotes cell death. BIRC6 directly inhibits caspase-3 and-7 and ubiquitinates caspase-3,-7, and-9, working with noncanonical E1, UBA6. SMAC suppresses both mechanisms by competitively displacing caspases.
Article
Multidisciplinary Sciences
Ronak Lakhia, Harini Ramalingam, Chun-Mien Chang, Patricia Cobo-Stark, Laurence Biggers, Andrea Flaten, Jesus Alvarez, Tania Valencia, Darren P. Wallace, Edmund C. Lee, Vishal Patel
Summary: This study demonstrates that removing the miR-17 binding element can enhance the expression of PKD1 and PKD2 genes, thereby alleviating the symptoms of autosomal dominant polycystic kidney disease and improving cellular function and mitochondrial membrane potential.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Huayu Zhu, Jun Li, Yize Li, Zhao Zheng, Hao Guan, Hongtao Wang, Ke Tao, Jiaqi Liu, Yunchuan Wang, Wanfu Zhang, Chao Li, Jie Li, Lintao Jia, Wendong Bai, Dahai Hu
Summary: This study reveals the roles of glucocorticoid in cellular mechanotransduction and a novel lncRNA-dependent GMD mechanism, offering a potential strategy for early intervention in mechanical disorder-associated diseases.
Article
Cell Biology
Rudian Zhang, Wenzhi Feng, Suhong Qian, Fei Wang
Summary: Autophagy plays an important role in regulating the meiosis-specific RNA-binding protein Rim4 and its associated transcripts in yeast meiosis. Rim4 enters the nucleus using a nuclear localization signal and loads its mRNA substrates before nuclear export. Active autophagy selectively spares the Rim4-mRNA complex in the cytoplasm, while degrading Rim4 during meiotic divisions in an Atg11-dependent manner, coinciding with the release of Rim4-bound mRNAs for translation. The conserved RNA recognition motifs (RRMs) of Rim4 are involved in stimulating Atg1 kinase and facilitating selective autophagy.
Article
Multidisciplinary Sciences
Marketa Sasinkova, Petr Herman, Ales Holoubek, Dita Strachotova, Petra Otevrelova, Dana Grebenova, Katerina Kuzelova, Barbora Brodska
Summary: Nucleophosmin mutations causing export from the nucleoli to the cytoplasm are common in AML, leading to altered functions. NSC348884 was studied as a potential inhibitor of NPM oligomerization, but was found to not inhibit oligomer formation, with cytotoxicity instead linked to cell adhesion signaling.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Maria E. Cardona, Jorma Hinkula, Kristin Gustafsson, Birger Christensson, Britta Wahren, Abdalla J. Mohamed, C. I. Edvard Smith, H. Jose Arteaga
Summary: Treatment with RNAi against HIV-1 transcripts can effectively inhibit viral replication but also leads to the selection of escape mutants. Blocking both viral and host transcripts improves the antiviral effect. The study demonstrates that short hairpin RNA (shRNA) targeting CCR5 provides more sustained protection than shRNA targeting HIV-1 rev. Partial reduction in CCR5 expression significantly decreases HIV-1 infection, and shCCR5 performs better than shRev in mixed treated and untreated cultures.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Multidisciplinary Sciences
Claire Maudet, Marouane Kheloufi, Sylvain Levallois, Julien Gaillard, Lei Huang, Charlotte Gaultier, Yu-Huan Tsai, Olivier Disson, Marc Lecuit
Summary: This study investigates the mechanisms by which pathogens access the brain and cause infections of the central nervous system. The researchers discovered that a bacterial surface protein, InIB, protects infected monocytes from immune cell-mediated death, thereby prolonging the lifespan of infected cells and promoting the entry of the pathogen into the brain.
Article
Multidisciplinary Sciences
Claire Maudet, Marouane Kheloufi, Sylvain Levallois, Julien Gaillard, Lei Huang, Charlotte Gaultier, Yu-Huan Tsai, Olivier Disson, Marc Lecuit
Summary: This study reveals a specific mechanism by which the bacterial pathogen Listeria monocytogenes confers an increased lifespan to infected cells by rendering them resistant to cell-mediated immunity, facilitating its persistence within the host, dissemination to the central nervous system, and transmission.
Article
Biochemistry & Molecular Biology
Juan B. Rodriguez-Molina, Francis J. O'Reilly, Holly Fagarasan, Eleanor Sheekey, Sarah Maslen, J. Mark Skehel, Juri Rappsilber, Lori A. Passmore
Summary: In this study, it was found that the Mpe1 subunit directly interacts with the polyadenylation signal sequence and promotes the activity of CPF, controlling polyadenylation. The Cft2 subunit of CPF antagonizes the stable configuration of Mpe1. The depletion or mutation of Mpe1 leads to widespread transcription termination defects and interference with neighboring genes.
Article
Genetics & Heredity
Jung-Ho Park, Munjin Kwon, Yoshihiro Yamaguchi, Bonnie L. Firestein, Ji-Young Park, Jieun Yun, Jeong-Ook Yang, Masayori Inouye
Article
Medicine, Legal
Jung-Ho Park, Jae-Woo Lee, Hoonsung Choi, Sun Keun Jung, Jeom Sun Kim, Kyung-Woon Kim, Keon Bong Oh, Hyeon Yang, Sung June Byun
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Hyeok-Won Lee, Jung-Ho Park, Hee-Suk Lee, Chun-Suk Kim, Jin-Gyeom Lee, Won-kyo Kim, Kyoung-Hwa Ryu, Jung-Oh Ahn, Eun-Gyo Lee, Seon-Won Kim, Jong-Min Jeon, Yung-Hun Yang, Eui-Sung Choi, Hong-Weon Lee
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2019)
Article
Biochemistry & Molecular Biology
Hyeok-Won Lee, Jung-Ho Park, Won-Kyo Kim, Jin-Gyeom Lee, Ju-Seok Lee, Jung-Oh Ahn, Eun-Gyo Lee, Hong-Weon Lee
Article
Microbiology
Wonho Choi, Yoshihiro Yamaguchi, Ji-Young Park, Sang-Hyun Park, Hyeok-Won Lee, Byung-Kwan Lim, Michael Otto, Masayori Inouye, Min-Ho Yoon, Jung-Ho Park
Summary: Agrobacterium tumefaciens utilizes a toxin-antitoxin system to control T-DNA transfer for producing genetically modified plants. The toxin MazF-at inhibits protein synthesis by decreasing cellular mRNA stability, while co-expression with antitoxin MazE-at restores normal cell growth. These findings suggest MazF-at as a potential ribosome-independent endoribonuclease for further applications.
Article
Biotechnology & Applied Microbiology
Y. Tsukuda, N. Mizuhara, Y. Usuki, Y. Yamaguchi, A. Ogita, T. Tanaka, K. Fujita
Summary: Anethole, a phenylpropanoid compound, shows synergistic antifungal activity when combined with other drugs, with potency being influenced by the length of the p-alkyl chain and the methoxy group of anethole. The presence of a double bond in the propenyl group did not significantly impact antifungal potency. Production of reactive oxygen species and inhibition of drug efflux also played roles in the antifungal activities observed.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Aritra Roy Choudhury, Ji-Young Park, Do Young Kim, Jeongyun Choi, Satabdi Acharya, Jung-Ho Park
Summary: This study evaluates the gut bacterial diversity of rainbow trout exposed to oxy-tetracycline and predicts the metabolic functioning of the bacterial community. The results show that antibiotic treatment leads to changes in gut bacterial community composition and affects certain metabolic processes.
Article
Agronomy
Yazhini Gunasekaran, Subramaniam Thiyageshwari, Manikandan Ariyan, Aritra Roy Choudhury, Jung-Ho Park, Duraisamy Selvi, Lakshmanan Chithra, Rangasamy Anandham
Summary: This study isolated exopolysaccharide-producing sodic tolerant bacteria (EPS-STB) and investigated their role in alleviating sodic stress in rice. The results showed that four selected bacterial isolates had the ability to promote rice growth under sodic conditions.
Article
Biochemistry & Molecular Biology
Wonho Choi, Jae-hui Kim, Ju Seok Lee, Jung-Ho Park
Summary: This study aimed to identify the TA system of Pseudomonas cichorii JBC1 genome and evaluate the mRNA expression levels under various stressful growth conditions. Two toxins, PCH70-01,410 and PCH70-16,540, were found to cause cell growth arrest and death in E. coli, and their toxicity was neutralized by their cognate antitoxins. Moreover, the mRNA expression levels of the TA systems differed under oxidative, acidic, and heat shocks.
MOLECULAR & CELLULAR TOXICOLOGY
(2023)
Article
Plant Sciences
Aritra Roy Choudhury, Pankaj Trivedi, Munusamy Madhaiyan, Jeongyun Choi, Wonho Choi, Jung-Ho Park, Denver I. Walitang, Tongmin Sa
Summary: This study evaluates the efficacy of ACC deaminase producing methylotrophic bacteria in enhancing cell viability of rice roots under salt stress. The results show that Methylobacterium oryzae strain CBMB20 enhances cell viability by regulating ethylene emission pathway. Additionally, the study reveals that senescence-associated protein (SAP) mRNA expression and enzyme activity are coordinated with major phytohormones. This research provides a mechanistic understanding of the plant-microbe interactions important for stress alleviation, where Methylobacterium spp. plays a central role in enhancing plant biomass through restriction of apoptosis via regulation of ethylene emission.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Anoth Maharjan, Jung-Ho Park
Summary: Cell-free protein synthesis (CFPS) system is an innovative technology that has a wide range of applications in the biomedical industry and can provide solutions to various environmental and health issues. Despite the obstacles that need to be overcome, its ease of use and versatility make it a vital tool in synthetic biology research and industry.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
So-Hee Kim, Ha-Hyeon Shin, Jin-Ho Kim, Jung-Ho Park, Eun-Seok Jeon, Byung-Kwan Lim
Summary: AKT2 plays a crucial role in CVB3-mediated myocarditis, as its knockout leads to cardiac damage, inflammation, and dysfunction, while suppressing the innate immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Mao Shaku, Jung-Ho Park, Masayori Inouye, Yoshihiro Yamaguchi
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Wonho Choi, Min-Ho Yoon, Jung-Ho Park
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2018)