Article
Oncology
Simon F. Merz, Philipp Jansen, Ricarda Ulankiewicz, Lea Bornemann, Tobias Schimming, Klaus Griewank, Zuelal Cibir, Andreas Kraus, Ingo Stoffels, Timo Aspelmeier, Sven Brandau, Dirk Schadendorf, Eva Hadaschik, Gernot Ebel, Matthias Gunzer, Joachim Klode
Summary: This study utilized LSFM technology to comprehensively analyze SLNs of melanoma patients, revealing metastases undetectable by routine histopathological assessment and improving the accuracy of detection and localization of metastatic cancer, potentially providing additional therapeutic options for patients.
EUROPEAN JOURNAL OF CANCER
(2021)
Article
Biotechnology & Applied Microbiology
Xuesong Li, Yicong Wu, Yijun Su, Ivan Rey-Suarez, Claudia Matthaeus, Taylor B. B. Updegrove, Zhuang Wei, Lixia Zhang, Hideki Sasaki, Yue Li, Min Guo, John P. P. Giannini, Harshad D. D. Vishwasrao, Jiji Chen, Shih-Jong J. Lee, Lin Shao, Huafeng Liu, Kumaran S. S. Ramamurthi, Justin W. W. Taraska, Arpita Upadhyaya, Patrick La Riviere, Hari Shroff
Summary: Enhanced axial resolution for 3D SIM is achieved with deep learning or four-beam interference. These methods allow for higher spatial frequency content and near-isotropic imaging with resolutions of around 120 nm in both lateral and axial directions. The deep learning method can also be combined with denoising for volumetric imaging. Various cellular samples were imaged to demonstrate the potential of these advancements.
NATURE BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Maha K. Rahim, Jinghui Zhao, Hinesh V. Patel, Hauna A. Lagouros, Rajesh Kota, Irma Fernandez, Enrico Gratton, Jered B. Haun
Summary: Fluorescence lifetime-based multiplexing allows simultaneous detection of multiple probes, and the phasor approach provides a simple solution for lifetime analysis. This study demonstrates the ability of phasor analysis to resolve and quantify exogenous fluorescent probes based on lifetime information alone. It also shows that lifetime-based quantitation accuracy can be improved through intensity matching. The results highlight the potential of fluorescence lifetime imaging and phasor analysis as a powerful tool for simultaneous detection of multiple biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Chudai Zeng, Zhuohui Chen, Haojun Yang, Yishu Fan, Lujing Fei, Xinghang Chen, Mengqi Zhang
Summary: Imaging technology, as an important method for diagnosing strokes and studying their pathological mechanisms, has undergone significant development and plays a vital role in stroke research.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Vinit Sheth, Xuxin Chen, Evan M. Mettenbrink, Wen Yang, Meredith A. Jones, Ons M'Saad, Abigail G. Thomas, Rylee S. Newport, Emmy Francek, Lin Wang, Alex N. Frickenstein, Nathan D. Donahue, Alyssa Holden, Nathan F. Mjema, Dixy E. Green, Paul L. DeAngelis, Joerg Bewersdorf, Stefan Wilhelm
Summary: Super-resolution microscopy was used to visualize the distribution of nanoparticles inside mammalian cells. By exploiting the light scattering properties of nanoparticles, label-free imaging of intracellular nanoparticles was achieved. This technology can be widely used to study the intracellular fate of nanoparticles and inform the development of safer and more effective nanomedicines.
Article
Optics
Cristina Polonschii, Mihaela Gheorghiu, Sorin David, Szilveszter Gaspar, Sorin Melinte, Hassaan Majeed, Mikhail E. Kandel, Gabriel Popescu, Eugen Gheorghiu
Summary: A novel multimodal functional imaging instrument has been developed in this study, enabling high-resolution mapping of optical path differences and electrical impedance variations. By combining phase imaging with electrical modulation, the system can achieve submicron spatial resolution for retrieving electro-optical maps with high spatial and temporal resolutions. This innovative optoelectrochemical method shows promise for a wider range of electrically modulated optical methods in measuring the electric field locally.
LIGHT-SCIENCE & APPLICATIONS
(2021)
Article
Biochemical Research Methods
Xiang Hao, Edward S. Allgeyer, Dong-Ryoung Lee, Jacopo Antonello, Katherine Watters, Julianne A. Gerdes, Lena K. Schroeder, Francesca Bottanelli, Jiaxi Zhao, Phylicia Kidd, Mark D. Lessard, James E. Rothman, Lynn Cooley, Thomas Biederer, Martin J. Booth, Joerg Bewersdorf
Summary: The combination of adaptive optics with an improved isoSTED nanoscope allows imaging of cells and tissues with sub-50-nm isotropic resolution. The advanced adaptive optics strategy used in the improved nanoscope enables sub-50-nm isotropic resolution of structures such as neuronal synapses and ring canals previously inaccessible in tissue.
Article
Engineering, Electrical & Electronic
Naeem Ullah, Muhammad Idrees, Muhib Ullah, Bakth Amin Bacha, Arif Ullah, Muhammad Haneef
Summary: Theoretical investigation of two-dimensional atomic microscopy using a unique configuration of three probe coherences and three standing-wave fields in a five-level atomic system reveals significantly improved localization behavior and multiple localized peaks in a single wavelength domain. High-resolution and high-precision 2D atomic microscopy with 100% localization probability in a specific region of 2D space is theoretically achieved in this proposed atomic system, which may have important implications in laser cooling, trapping of neutral atoms, and nano-lithography.
OPTICAL AND QUANTUM ELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Ying Chen, Jhih-Heng Yang, Ya-Ting Chang, I-Ming Lin, Chien-Nan Hsiao, Yeo-Wan Chiang, Chien-Chun Chen
Summary: Block copolymer-based multicomponent materials have attracted attention due to their tunable properties. Electron tomography through TEM is an established method for the 3D imaging of stained block copolymers. STEM with an ADF detector has emerged as a method for the 3D structural analysis of unstained block copolymers. However, the lack of electron contrast has limited the resolution of 3D reconstructions for light elements. In this study, the first 3D structural analysis of unstained double-gyroid block copolymers was achieved using Cs-corrected STEM at a resolution of 8.6 nm.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Physical
Huipeng Ma, Feng Lin, Shibo Cheng, Jin-Dou Huang
Summary: In this study, the electronic structures and conducting properties of BN-embedded perylene diimides (B2N2-PDIs) were systematically investigated. The substitution of C-C units with B-N units in the pi-electron core was found to significantly affect the reorganization energies, ionization potential, and electron affinity of the PDIs. It was also revealed that B2N2-PDIs have the potential to be developed as high-efficiency n-type organic semiconductor materials. Additionally, the three-dimensional angular resolution anisotropic mobility of 6-CH, 6-EH, and 6-DIPP was simulated for the first time, and their charge carrier-transport properties were characterized.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Oncology
Laura Furia, Simone Pelicci, Federica Perillo, Maddalena M. Bolognesi, Pier Giuseppe Pelicci, Federica Facciotti, Giorgio Cattoretti, Mario Faretta
Summary: In situ multiplexing analysis and in situ transcriptomics are revolutionary tools for understanding the molecular basis of cancer and advancing personalized medicine. To achieve the best resolution and sensitivity, continuous interplay between different technologies is required. We propose a new experimental pipeline that alternates between automated and manual procedures to optimize the cost-to-benefit ratio in multiplexing experiments.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Maik Kahnt, Lukas Grote, Dennis Brueckner, Martin Seyrich, Felix Wittwer, Dorota Koziej, Christian G. Schroer
Summary: Ptychographic X-ray microscopy is ideal for observing chemical processes in situ, but the thickness of samples can limit lateral resolution in traditional reconstruction models. By adopting a multi-slice reconstruction approach, higher spatial resolution can be achieved.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Natalie Aroha Holroyd, Claire Walsh, Lucie Gourmet, Simon Walker-Samuel
Summary: Episcopic imaging techniques, such as HREM, have enabled three-dimensional visualization of biological samples. This systematic review analyzes trends in quantitative analysis of episcopic images and discusses the need for further research. The analysis highlights the increasing use of quantitative processing and manual image annotation. Further development in tools and methods, as well as automation and standardization of annotation and quantification, are identified as important for the future of quantitative episcopic imaging.
Article
Engineering, Mechanical
Wujun Yin, Fabien Briffod, Haoyu Hu, Takayuki Shiraiwa, Manabu Enoki
Summary: In this study, high-resolution digital image correlation and laser scanning confocal microscopy were used to investigate the deformation behaviors of polycrystalline austenitic stainless steels at the sub-grain scale. The results showed that slip and mechanical twinning activities occurred at the early stage of yielding and increased with the applied load. The presence of nanotwins hindered slip glide, resulting in twin-slip interaction and grain boundary strengthening. Furthermore, the observation of out-of-plane roughness provided insights into the significant deformation gradient near boundaries.
INTERNATIONAL JOURNAL OF PLASTICITY
(2023)
Article
Biochemical Research Methods
Weikai Xue, Jonas Ogien, Pavel Bulkin, Anne-Lise Coutrot, Arnaud Dubois
Summary: This study aimed to develop a more compact and lighter LC-OCT device that can acquire images faster without significant degradation of resolution. The device is based on a Mirau interferometer using a single objective and incorporates a galvanometer scanner for lateral scanning of the illumination line. The optimized device can acquire B-scans at an unprecedented rate of 17 fps and produce 3D images of human skin at cellular resolution.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Article
Biochemistry & Molecular Biology
Viviane Lutz-Bueno, Ana Diaz, Tingting Wu, Gustav Nystroem, Thomas Geiger, Carlo Antonini
Summary: This study demonstrates the potential of X-ray scattering imaging for hierarchical characterization of biopolymer materials at different scales, including the macroscopic, nanoscopic, and molecular scales.
Article
Materials Science, Multidisciplinary
T. Chommaux, P. O. Renault, D. Thiaudiere, P. Godard, F. Paumier, T. Girardeau, S. Hurand, P. H. Goudeau
Summary: The electrical and mechanical properties of four different ITO films were investigated. It was found that these films have low elastic anisotropy and are very brittle during straining. The introduction of oxygen flow and decrease in deposition temperature delayed the onset of cracking.
Article
Chemistry, Multidisciplinary
Sebastian Weber, Ana Diaz, Mirko Holler, Andreas Schropp, Mikhail Lyubomirskiy, Ken L. Abel, Maik Kahnt, Arno Jeromin, Satishkumar Kulkarni, Thomas F. Keller, Roger Glaser, Thomas L. Sheppard
Summary: This study utilizes in situ 2D hard X-ray ptychography (XRP) and 3D ptychographic X-ray computed tomography (PXCT) to investigate the synthesis process of hierarchically porous materials. The XRP allows for the monitoring of textural changes, while PXCT provides quantitative information on pore structures. The findings are corroborated with traditional pore characterization methods, leading to a better understanding of the synthesis pathways of porous materials.
Article
Pharmacology & Pharmacy
Arto Merivaara, Jere Kekkonen, Julia Monola, Elle Koivunotko, Marko Savolainen, Tuomo Silvast, Kirsi Svedstrom, Ana Diaz, Mirko Holler, Ossi Korhonen, Marjo Yliperttula, Sami Valkonen
Summary: Near-infrared spectroscopy can be used to monitor the residual water content of freeze-dried products, and our predictive model accurately predicts the residual water content of different biomaterial aerogels. This is important for the freeze-drying of biomaterials and aerogel fabrication.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Optics
Frederic Zolla, Pierre Godard
Summary: This tutorial introduces in a natural fashion the propagation equations system governing the scattering of an electromagnetic wave by a nonlinear medium. It aims to obtain the equations showing commonly observed nonlinear effects with minimum assumptions. The tutorial discusses the energy transfer between fields emitted at different frequencies and provides numerical examples of scattering by nonlinear materials.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2022)
Article
Biochemistry & Molecular Biology
Isabella Silva Barreto, Maria Pierantoni, Malin Hammerman, Elin Tornquist, Sophie Le Cann, Ana Diaz, Jonas Engqvist, Marianne Liebi, Pernilla Eliasson, Hanna Isaksson
Summary: The structural response of collagen fibrils in Achilles tendons during load to rupture, cyclic loading, and stress relaxation was investigated using in situ loading and small-angle X-ray scattering. The fibrils exhibited lower strain than the applied tissue strain, and showed linear strain in the elastic region of the tissue. During cyclic loading, the fibrils demonstrated increased stretchability and recovery, while during tissue stress relaxation, they showed relaxation of fibril strain. Fibril strain heterogeneity increased with applied loads and became increasingly disordered after the toe region.
Article
Chemistry, Physical
Ezgi Yildirim, Raj Kumar Ramamoorthy, Rohan Parmar, Pierre Roblin, Jorge A. Vargas, Valeri Petkov, Ana Diaz, Stefano Checchia, Isaac Rodriguez Ruiz, Sebastien Teychene, Lise -Marie Lacroix, Guillaume Viau
Summary: Currently, there is a strong focus on studying ultrasmall metal nanoparticles due to their unique properties. The synthesis of monodisperse nanoparticles of a very small size and well-defined structure requires a good understanding of the crystallization process, which can be achieved by conducting in situ studies. This article describes the synthesis of ultrasmall gold nanoparticles using reduction methods and reveals the competition between nucleation and crystallization processes. By adjusting reaction conditions, such as concentration and temperature, monodisperse gold nanoparticles with icosahedral structure can be obtained.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Multidisciplinary Sciences
Shiva Shirani, Ana Cuesta, Alejandro Morales-Cantero, Isabel Santacruz, Ana Diaz, Pavel Trtik, Mirko Holler, Alexander Rack, Bratislav Lukic, Emmanuel Brun, Ines R. Salcedo, Miguel A. G. Aranda
Summary: Despite decades of research, our understanding of cement dissolution and precipitation processes at early stages is still limited due to the lack of suitable imaging methods. In this study, near-field ptychographic nanotomography was used to visualize the hydration of commercial Portland cement in a thick capillary. The results show the formation of a porous C-S-H gel shell with a thickness of 500 nm after 19 hours, covering all the alite grains with a water gap. The dissolution rate of smaller alite grains was found to be approximately four times faster than that of larger grains in the deceleration stage. The study also mapped out the development of etch pits and measured particle size distributions over time. This work lays the foundation for mechanistic studies of dissolution-precipitation processes in cement using advanced nanoimaging techniques.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Pierre Godard, Mariana Verezhak, Tarik Sadat, Florent Mignerot, Vincent L. R. Jacques, David Le Bolloc'h, Carsten Richter, Felisa Berenguer, Ana Diaz, Steven Van Petegem, Pierre-Olivier Renault, Ludovic Thilly
Summary: Micropillar compression is a useful method for understanding mechanics on a small scale. This article explores the use of diffraction with a coherent X-ray beam to study the behavior of InSb micropillars during compression. The experiments show the ability to determine the plastic regime and study the spatially resolved plastic strain fields. However, limitations arise in quantitatively measuring the elastic strain field due to the similar diffraction angles from the pedestal and micropillar.
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2023)
Article
Materials Science, Multidisciplinary
Yen Fred Woguem, Pierre Godard, Julien Durinck, Sandrine Brochard
Summary: The study investigates the role of twin boundaries in enhancing the properties of materials, focusing on Sigma 3{111} coherent twin boundaries (CTBs) and Sigma 3{112} symmetric incoherent twin boundaries (ITBs) in gold. Multiple atomistic simulations and density functional theory are employed to characterize these defects. The results reveal that the excess volumes induced by twin boundaries are highly dependent on the interatomic potentials used. The formation energy and elastic deformation of the resulting 9R phase are also analyzed to provide a comprehensive understanding of the twin boundary effects.
Article
Engineering, Biomedical
Maria Pierantoni, Isabella Silva Barreto, Malin Hammerman, Vladimir Novak, Ana Diaz, Jonas Engqvist, Pernilla Eliasson, Hanna Isaksson
Summary: This study investigates the effects of reduced loading on the structural properties and mechanical response of rat Achilles tendons. The results show that reduced loading leads to more crimped and less organized fibers, which could explain the altered mechanical response.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Applied
Ines Kutzli, Viviane Lutz-Bueno, Massimo Bagnani, Ana Diaz, Hamed Almohammadi, Reed A. Nicholson, Stefan K. Baier, Raffaele Mezzenga
Summary: This study investigates the fabrication of core-shell microfibers using microfluidic spinning of pectin and beta-lactoglobulin. The addition of amyloid fibrils as the protein core improved the mechanical properties of the fibers, but increasing the protein core flow rate had a negative effect. Overall, full length amyloid fibrils showed better mechanical properties than shortened amyloid fibrils.
FOOD HYDROCOLLOIDS
(2023)
Article
Pharmacology & Pharmacy
Martina Olsson, Rydvikha Govender, Ana Diaz, Mirko Holler, Andreas Menzel, Susanna Abrahmsen-Alami, Matthew Sadd, Anette Larsson, Aleksandar Matic, Marianne Liebi
Summary: This paper presents a correlative, multiscale imaging methodology combining PXCT and S/WAXS to visualize and quantify the morphology of solid dosage forms. The method allows characterization of structures from the nanometre to millimetre regime. The methodology is demonstrated using a hot-melt extruded solid dispersion of carbamazepine in ethyl cellulose. The 3D morphology was visualized through PXCT, revealing an oriented structure of crystalline drug domains. Scanning S/WAXS showed similarity in the nanostructure across the cross section of the extruded filament.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemical Research Methods
Raj Kumar Ramamoorthy, Ezgi Yildirim, Isaac Rodriguez-Ruiz, Pierre Roblin, Lise-Marie Lacroix, Ana Diaz, Rohan Parmar, Sebastien Teychene, Guillaume Viau
Summary: We present a continuous microreactor platform that allows sub-millisecond homogeneous reagent mixing for the synthesis of ultra-small gold nanoparticles. By utilizing high frequency time-resolved information, we observed the presence of Au(iii)/Au(i) prenucleation clusters and the transient formation of an Au(i) lamellar phase. Two different reaction pathways associated with the presence or absence of the Au(i) lamellar phase are proposed and modeled.