Article
Chemistry, Multidisciplinary
Sebastian Dengler, Celine Douat, Ivan Huc
Summary: In this study, hybrid sequences consisting of a peptide with multiple Cys residues and an aromatic foldamer helix with multiple chloroacetamide functions were synthesized. The reactive sites on the structurally defined helix were strategically placed to selectively stitch the peptide to form different macrocycles, resulting in mostly one preferred product. The reaction trails, involving identical reaction sites successively due to precise positioning in space, can be predicted based on structural considerations and the assumption that smaller macrocycles form faster.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Tanya Ju-Ngam, Nichanun McMillan, Mamoru Yoshimizu, Hisae Kasai, Ratree Wongpanya, Prapansak Srisapoome
Summary: This research conducted molecular characterization and biofunctional analyses of Hsp40 and Hsp90 genes in giant river prawns under various stress conditions. The highest similarity scores of these genes were found with crustaceans. Expression patterns of Mr-hsp40 and Mr-hsp90 were different under Aeromonas hydrophila challenge and heat-cold shock conditions, highlighting their significant roles in stress responses.
Review
Biochemistry & Molecular Biology
Siarhei A. Dabravolski, Vasily N. Sukhorukov, Vladislav A. Kalmykov, Nikolay A. Orekhov, Andrey V. Grechko, Alexander N. Orekhov
Summary: Cardiovascular diseases are a major cause of death worldwide, with molecular chaperones like HSP90 playing a crucial role in heart protection and aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biology
Xun Fu, Jiang Liu, Xin Yan, Michael E. DiSanto, Xinhua Zhang
Summary: Prostate cancer is the second most common cancer in aging men globally, but its exact pathogenesis is not fully understood. The heat shock protein (HSP) family plays a significant role in promoting tumor growth and protecting cancer cells from death. HSP molecules are closely involved in important biological processes such as cell differentiation, epithelial-mesenchymal transition, and fibrosis. Agents and inhibitors targeting the critical structure of HSP molecules have been developed for their potent antitumor effects. However, only a small number of them have been clinically tested. Further clinical studies are needed to establish the relationship between HSP70 family, HSP90 molecule, and prostate cancer treatment.
Article
Oncology
Enzo M. Scutigliani, Yongxin Liang, Marloes IJff, Hans Rodermond, Xionge Mei, Miriam P. Korver, Vaneesha S. Orie, Ron A. Hoebe, Daisy Picavet, Arlene Oei, Roland Kanaar, Przemek M. Krawczyk
Summary: Summary Hyperthermia can improve the effects of radio- and chemotherapy, but its efficacy is limited by the heat stress response (HSR). In this study, we evaluated the HSP90 inhibitor ganetespib in promoting the effects of radiotherapy or cisplatin combined with hyperthermia. Our results showed that ganetespib could enhance the efficacy of hyperthermia when combined with radiation.
Article
Multidisciplinary Sciences
Tsukumi Miwa, Hideki Taguchi
Summary: Research has shown that IbpA down-regulates the expression of multiple heat shock proteins (Hsps) by repressing the translation of the heat shock transcription factor sigma(32), and plays a crucial role in shutting down the heat shock response. These findings reveal an unknown role of IbpA in regulating the heat shock response at the translational level.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Veterinary Sciences
Samuel A. Atkin, Abu Saleh Md Moin, Stephen L. Atkin, Alexandra E. Butler
Summary: This study investigated the effects of hypoglycemia on the heat shock-related protein pathway. The results showed that hypoglycemia significantly decreased the expression of HSP90, 70, 27, and ubiquitin proteins, which are important for the protective response against heat stress.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Medicine, Research & Experimental
Ho Jin Lee, Hye-Young Min, Young-Sik Yong, Jihyae Ann, Cong Truong Nguyen, Minh Thanh La, Seung Yeob Hyun, Huong Thuy Le, Hyewon Kim, Hyukjin Kwon, Gibeom Nam, Hyun-Ju Park, Jeewoo Lee, Ho-Young Lee
Summary: This study revealed that Hsp90 inhibitors that block the N-terminal ATP-binding pocket activate STAT3, leading to the transcriptional upregulation of Wnt ligands and promoting cell survival in NSCLC. NCT-80 effectively reduced NSCLC cell viability, migration, and CSC-like phenotypes, and inhibited the growth of patient-derived xenograft tumors.
Article
Plant Sciences
Quanquan Shen, Qian Chen, Yang Liu, Xiang Xue, Xiaogang Shen, Qiang He, Guokun Wang, Fei Han
Summary: Aspirin can partially alleviate vascular smooth muscle cell (VSMC) calcification by regulating the expression of heat shock protein 70 (HSP70) and heat shock protein 90 (HSP90). Blocking HSP70 or HSP90 inhibitors can partially weaken the anti-calcification effect of aspirin.
PHARMACEUTICAL BIOLOGY
(2022)
Review
Plant Sciences
Yunzhuan Zhou, Fuxiang Xu, Yanan Shao, Junna He
Summary: As global warming intensifies, the earth's average temperature continues to rise. Plants have developed two main mechanisms, the heat stress response and the thermomorphogenesis response, to cope with elevated temperatures. This review summarizes recent progress in understanding these heat-responsive molecular regulatory pathways in plants and discusses future perspectives in this field.
Review
Microbiology
Seongjoon Moon, Soojeong Ham, Juwon Jeong, Heechan Ku, Hyunhee Kim, Changhan Lee
Summary: Temperature is critical for the survival of all living organisms. Bacteria, being unicellular organisms, have evolved sensitive mechanisms to sense and defend against temperature changes. This review focuses on the molecular perspective of cellular changes and bacterial responses, particularly in Escherichia coli, when subjected to temperature shifts.
JOURNAL OF MICROBIOLOGY
(2023)
Article
Parasitology
Desmond O. Agwunobi, Tongxuan Wang, Meng Zhang, Tianhong Wang, Qingying Jia, Miao Zhang, Xinyue Shi, Zhijun Yu, Jingze Liu
Summary: In this study, genes of hsp70 and hsp90 cDNA were cloned and characterized from the tick Dermacentor silvarum, and their functions in cold stress were evaluated. Results showed that Dshsp70 and Dshsp90 play essential roles in the low-temperature adaptation of ticks. This study contributes to understanding the survival and acclimatization of overwintering ticks.
PARASITES & VECTORS
(2021)
Article
Environmental Sciences
Shao-Shuai Bi, Milton Talukder, Xue-Tong Sun, Mei-Wei Lv, Jing Ge, Cong Zhang, Jin-Long Li
Summary: This study assessed the neurotoxic effects of chronic cadmium (Cd) exposure on the chicken cerebellum and investigated the expression of genes related to the heat-shock response. The results showed that Cd exposure led to clinical symptoms of ataxia in the chickens, decreased the number of Purkinje cells, and induced degeneration of Purkinje cells. Cd exposure also affected the expression of heat-shock factors and proteins. These findings shed light on the toxic effects of Cd on the cerebellum and provide evidence for the health risks posed by Cd exposure.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Plant Sciences
Yunxie Wei, Hongqiu Zeng, Wen Liu, Xiao Cheng, Binbin Zhu, Jingru Guo, Haitao Shi
Summary: This study systematically analyzed the correlation between HSP90 and autophagy signaling in cassava, and investigated their effects on plant disease response and underlying mechanisms through functional genomics and genetic phenotype assay. The study found that the potential MeHSP90.9-MeSGT1-MeRAR1 chaperone complex interacts with MeATGs and triggers autophagy signaling, enhancing disease resistance to cassava bacterial blight.
Article
Biochemistry & Molecular Biology
Raquel Alves, Diogo Santos, Joana Jorge, Ana Cristina Goncalves, Steve Catarino, Henrique Girao, Joana Barbosa Melo, Ana Bela Sarmento-Ribeiro
Summary: Heat shock protein 90 (HSP90) plays a crucial role in stabilizing and protecting client proteins. Alvespimycin, an HSP90 inhibitor, has shown potential in overcoming imatinib resistance in chronic myeloid leukemia (CML) cell lines. This study found that Alvespimycin effectively reduced metabolic activity and induced cell death, apoptosis, and cell cycle arrest in imatinib-sensitive and imatinib-resistant CML cells. It also increased HSP70 expression as a marker of HSP90 inhibition. These findings suggest that Alvespimycin could be a promising therapeutic approach for CML treatment, even in cases of imatinib resistance.
Article
Cardiac & Cardiovascular Systems
Yen Chin Koay, Kelly Stanton, Vivian Kienzle, Mengbo Li, Jean Yang, David S. Celermajer, John F. O'Sullivan
Summary: This study examined the metabolic adaptation to an 80-day exercise intervention in healthy young male adults with controlled lifestyle factors. The results showed a significant shift in global metabolism, with decreases in fatty acids and ketone bodies in response to increased aerobic fitness. A variety of metabolic substrates experienced significant changes, indicating the extensive impact of exercise on metabolism.
CARDIOVASCULAR RESEARCH
(2021)
Article
Agronomy
Charles L. Cantrell, Mohamed A. Zaki, Amber Reichley, Matt Sink, Seong J. Kim, Abbas Ali
Summary: The study discovered that a series of ester analogs of undecanoic acid and dodecanoic acid showed promising biting deterrent effects against the yellow fever mosquito. These compounds could potentially become new sources of mosquito-repelling compounds and warrant further investigation.
PEST MANAGEMENT SCIENCE
(2021)
Article
Neurosciences
Ben Stocks, Stephen P. Ashcroft, Sophie Joanisse, Linda C. Dansereau, Yen Chin Koay, Yasir S. Elhassan, Gareth G. Lavery, Lake-Ee Quek, John F. O'Sullivan, Ashleigh M. Philp, Gareth A. Wallis, Andrew Philp
Summary: Acute supplementation of nicotinamide riboside (NR) does not affect substrate metabolism or NAD(+)-sensitive signaling pathways in human skeletal muscle during or after endurance exercise. Although NR supplementation does not increase skeletal muscle NAD(+) concentration, it does affect the concentration of NAD(+) metabolome, demonstrating skeletal muscle bioavailability of NR supplementation.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Multidisciplinary Sciences
Jason Behary, Nadia Amorim, Xiao-Tao Jiang, Anita Raposo, Lan Gong, Emily McGovern, Ragy Ibrahim, Francis Chu, Carlie Stephens, Hazem Jebeili, Vincenzo Fragomeli, Yen Chin Koay, Miriam Jackson, John O'Sullivan, Martin Weltman, Geoffrey McCaughan, Emad El-Omar, Amany Zekry
Summary: The study demonstrates that patients with NAFLD-HCC have dysbiosis in the gut microbiota, leading to modulation of peripheral immune responses and the induction of a T cell immunosuppressive phenotype.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Stephen T. Vernon, Owen Tang, Taiyun Kim, Adam S. Chan, Katharine A. Kott, John Park, Thomas Hansen, Yen C. Koay, Stuart M. Grieve, John F. O'Sullivan, Jean Y. Yang, Gemma A. Figtree
Summary: The study identified novel associations between four metabolites and CAD plaque phenotypes, as well as two metabolic pathways strongly associated with CAD independently of traditional risk factors. These findings provide new insights and directions for further research in the field.
Article
Multidisciplinary Sciences
Taiyun Kim, Owen Tang, Stephen T. Vernon, Katharine A. Kott, Yen Chin Koay, John Park, David E. James, Stuart M. Grieve, Terence P. Speed, Pengyi Yang, Gemma A. Figtree, John F. O'Sullivan, Jean Yee Hwa Yang
Summary: A new computational workflow is developed to remove unwanted data variation while preserving biologically relevant information in large scale metabolomics studies.
NATURE COMMUNICATIONS
(2021)
Article
Cardiac & Cardiovascular Systems
Shashwati Dutta, Desmond Li, Andy Wang, Mark Ishak, Kristina Cook, Melissa Farnham, Hasthi Dissanayake, Peter Cistulli, Imre Hunyor, Renping Liu, Ian Wilcox, Yen Chin Koay, Jean Yang, Sean Lal, John F. O'Sullivan
Summary: Metabolites were found to be associated with cardiac function, LV wall thickness, and mass, while nicotine metabolite cotinine was associated with increased time spent with a sleep oxygen saturation less than 90%, indicating a significant hazard for smokers with sleep apnoea.
Article
Cardiac & Cardiovascular Systems
Desmond K. Li, Lauren E. Smith, Alexander W. Rookyard, Shivanjali J. Lingam, Yen C. Koay, Holly P. McEwen, Stephen M. Twigg, Anthony S. Don, John F. O'Sullivan, Stuart J. Cordwell, Melanie Y. White
Summary: The study investigates the impact of high-fat diet and streptozotocin on the metabolome and proteome in diabetic hearts, revealing changes in metabolism and protein expression that contribute to poor cardiovascular outcomes in type 2 diabetes.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Pathology
Paul Bonnitcha, David Sullivan, Michael Fitzpatrick, Andrea Ireland, Van Long Nguyen, Yen Chin Koay, John O'Sullivan
Summary: In this study, a hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-LC-MS/MS) method was developed for the simultaneous quantification of ADGV, ADMA, and SDMA. The method showed stability and excellent performance under routine laboratory conditions, making it suitable for investigating cardiovascular disease and NAFLD.
Article
Cell Biology
Travis Ruan, Dylan Harney, Yen Chin Koay, Lipin Loo, Mark Larance, Leslie Caron
Summary: Skeletal muscle weakness is associated with negative health outcomes. Current research lacks adequate cellular models, but this study developed a scalable monolayer system to differentiate human embryonic stem cells into mature skeletal muscle cells within 26 days. The combination treatment of testosterone, follistatin, and a cocktail of myokines further enhanced muscle cell differentiation and fusion. This cellular model provides a powerful tool for studying muscle development and drug discovery.
Article
Cardiac & Cardiovascular Systems
Mark Dennis, Sashie Howpage, Margaret McGill, Shashwati Dutta, Yen Koay, Lisa Nguyen-Lal, Sean Lal, Ted Wu, Martin Ugander, Alexandra Wang, Phillip A. Munoz, Jencia Wong, Maria I. Constantino, John F. O'Sullivan, Stephen M. Twigg, Rajesh Puranik
Summary: This study aims to identify biomarkers of cardiomyopathy in T2DM patients and explore the associations between functional status, metabolomic profile, and myocardial fibrosis. The results show that patients with well-controlled T2DM exhibit myocardial fibrosis, which is associated with reduced exercise tolerance and specific metabolites.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alison W. S. Luk, Lachlan Mitchell, Yen Chin Koay, John F. O'Sullivan, Helen O'Connor, Daniel A. Hackett, Andrew Holmes
Summary: This pilot study explored the dynamics between diet, exercise, and the gut microbiome in bodybuilders. The study found that bodybuilders successfully reduced fat mass and maintained lean mass during competition preparation through diet and exercise. Individual food choices were associated with gut microbiome composition, and post-competition dietary changes were associated with changes in gut microbiome diversity.
Article
Biochemistry & Molecular Biology
Yen Chin Koay, Adelle C. F. Coster, Daniel L. Chen, Brad Milner, Amani Batarseh, John F. O'Sullivan, Jerry R. Greenfield, Dorit Samocha-Bonet
Summary: This study identified diverse insulin resistance and adiposity phenotypes in men and women with obesity, which may enable targeted treatments for better health outcomes.
Article
Immunology
Benita C. Y. Tse, Angela L. Ferguson, Yen Chin Koay, Georges E. Grau, Anthony S. Don, Scott N. Byrne
Summary: The ability of UV radiation to suppress the immune system has both beneficial and detrimental effects. This study identified six lipids that are increased in UV-exposed mice and preferentially located in T cell areas. These lipids were found to play an immune regulatory role by inhibiting T cell proliferation. This research is important in understanding the mechanisms of UV-mediated immune suppression.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Endocrinology & Metabolism
Jibran A. Wali, Annabelle J. Milner, Alison W. S. Luk, Tamara J. Pulpitel, Tim Dodgson, Harrison J. W. Facey, Devin Wahl, Melkam A. Kebede, Alistair M. Senior, Mitchell A. Sullivan, Amanda E. Brandon, Belinda Yau, Glen P. Lockwood, Yen Chin Koay, Rosilene Ribeiro, Samantha M. Solon-Biet, Kim S. Bell-Anderson, John F. O'Sullivan, Laurence Macia, Josephine M. Forbes, Gregory J. Cooney, Victoria C. Cogger, Andrew Holmes, David Raubenheimer, David G. Le Couteur, Stephen J. Simpson
Summary: In this study, Simpson and colleagues investigate the impact of carbohydrate quality on protein-diluted diets in mice. They find that the type and digestibility of carbohydrates have a profound effect on metabolic outcomes, nutrient processing, and gut microbiota in mice. Low-protein, high-carbohydrate diets with resistant starch promote healthier metabolic responses, while a 50:50 mixture of fructose and glucose leads to the worst outcomes.
