期刊
ACS MEDICINAL CHEMISTRY LETTERS
卷 2, 期 9, 页码 698-702出版社
AMER CHEMICAL SOC
DOI: 10.1021/ml200120m
关键词
Diversity-oriented synthesis; screening collection; diabetes; beta cell; apoptosis
资金
- NIGMS [P50 GM069721]
- NIH [RL1CA133834, RL1HG004671, RL1GM084437, UL1DE019585]
- NIH-MLPCN [1 U54 HG005032-1]
- NIH-NIDDK
The synthesis of a stereochemically diverse library of medium-sized rings accessible via a build/couple/pair strategy is described. Key aspects of the synthesis include SNAr cycloetherification of a linear amine template to afford eight stereoisomeric eight-membered lactams and subsequent solid-phase diversification of these scaffolds to yield a 6488-membered library. Screening of this compound collection in a cell-based assay for the suppression of cytokine-induced beta-cell apoptosis resulted in the identification of a small-molecule suppressor capable of restoring glucose-stimulated insulin secretion in a rat beta-cell line. The presence of all stereoisomers in the screening collection enabled preliminary determination of the structural and stereochemical requirements for cellular activity, while efficient follow-up chemistry afforded BRD0476 (probe ML187), which had an approximately 3-fold increase in activity. These results demonstrate the utility of diversity-oriented synthesis to probe discovery using cell-based screening and the importance of including stereochemical diversity in screening collections for the development of stereo/structure-activity relationships.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据