期刊
EXPERIMENTAL AND THERAPEUTIC MEDICINE
卷 3, 期 1, 页码 80-86出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2011.364
关键词
hepatocarcinoma; Chinese herbal formula; apoptosis; cell senescence
资金
- Science and Technology Commission of Shanghai Municipality [09JC1413600]
- State Clinical Research Center of TCM [LYTD-04]
- Key Discipline of State Administration of Traditional Chinese Medicine (Traditional Chinese Medicine in Oncology)
- Shanghai Shen Kang Platform [SHDC12007206]
Liver cancer ranks as the fifth most prevalent malignancy of all cancers worldwide. According to the principles of traditional Chinese medicine, liver Yin deficiency is a common clinical syndrome of liver cancer, and tonifying liver Yin is a common treatment method for liver cancer. However, no hepatocarcinoma-specific liver Yin tonifying formula has yet been established. In the present study, we established a liver cancer-specific combination of herbs, which we term liver Yin tonifying formula (LYTF). We found that LYTF inhibits the proliferation of Bel-7402 cells in a dose- and time-dependent manner. LYTF induces apoptosis in Bel-7402 cells, which is accompanied by activation of caspases-8, -9 and -3. Pan-caspase blocking completely abrogates LYTF-induced apoptosis and partially abrogates LYTF-induced proliferation inhibition. LYTF also induces cell senescence, as indicated by a large and flattened morphology, senescence-activated beta-galactosidase-positive staining and G0/G1 cell cycle arrest, accompanied by the up-regulation of p16 and p21 and the down-regulation of retinoblastoma protein phosphorylation. These findings suggest that LYTF is effective in inhibiting the growth and survival of hepatocarcinoma cells through the induction of apoptosis and cell senescence. Our study also provides insight into traditional Chinese medicine methods used for the treatment of liver cancer.
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