Review
Cardiac & Cardiovascular Systems
Han Jiang, Lihua Li, Lili Zhang, Guangyao Zang, Zhen Sun, Zhongqun Wang
Summary: This study reviews the pathogenesis of vascular calcification, the role of endothelial cells, and the regulatory factors of EndMT, in order to provide new concepts for the prevention and treatment of VC.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Medicine, General & Internal
Daniel Neunhauserer, Alessandro Patti, David Niederseer, Bernhard Kaiser, Janne Cadamuro, Bernd Lamprecht, Andrea Ermolao, Michael Studnicka, Josef Niebauer
Summary: This study demonstrated that exercise training has beneficial effects on exercise capacity, systemic/eosinophilic inflammation, and endothelial function in nonhypoxemic COPD patients. Patients with endothelial dysfunction showed a significant reduction in white blood cells after exercise training.
AMERICAN JOURNAL OF MEDICINE
(2021)
Review
Cell Biology
Jenita Immanuel, Sanguk Yun
Summary: The physiological functions of endothelial cells play a crucial role in maintaining a healthy vascular system. However, various cardiovascular diseases are characterized by endothelial cell dysfunction triggered by external stimuli such as disturbed flow, hypoxia, growth factors, and cytokines. This review focuses on the main proinflammatory stimuli and their effects on endothelial cell function, as well as the impact of endothelial cell dysfunction on vascular diseases and potential targets for intervention.
Article
Cell & Tissue Engineering
Faiza Farhan, Manjari Trivedi, Priscilla Di Wu, Wei Cui
Summary: This study found that HLCs cultured with collagen showed increased albumin and alpha-1 anti-trypsin expression, reduced AFP, and higher urea secretion compared to HLCs cultured with Matrigel. However, collagen-cultured HLCs exhibited lower CYP3A4 activity and glycogen storage than Matrigel-cultured HLCs. The functional differences in HLCs between collagen and Matrigel cultures resemble hepatic zonation in the liver lobules.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Lea Flippe, Anne Gaignerie, Celine Serazin, Olivier Baron, Xavier Saulquin, Ignacio Anegon, Laurent David, Carole Guillonneau
Summary: Immunotherapy using primary T cells has greatly improved medical care in recent years, but limitations such as challenging cell genome editing and lack of standardized products have restricted its clinical use. The use of T cells derived from human pluripotent stem cells offers advantages of genetic modification and standardized allogeneic products. However, more research is needed to fully understand the feasibility and functionality of these T cells before clinical applications. This study successfully generated T cells from induced pluripotent stem cells, demonstrating the potential for therapeutic use and treatment of various diseases.
Article
Cell Biology
Susov Dhakal, Shahar Rotem-Bamberger, Josilyn R. Sejd, Meyrav Sebbagh, Nathan Ronin, Ruth A. Frey, Mya Beitsch, Megan Batty, Kineret Taler, Jennifer F. Blackerby, Adi Inbal, Deborah L. Stenkamp
Summary: The study on zebrafish embryos revealed that the depletion of vascular endothelial cells resulted in disorganized retinal layers and cell death, while the lack of blood flow led to reduced proliferation and differentiation in the retina. These findings emphasize the importance of vascular endothelial cells in retinal development and suggest distinct roles for them compared to circulating factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yaqi Tang, Lu Yin, Shuai Gao, Xiaojing Long, Zhanhui Du, Yingchao Zhou, Shuiyan Zhao, Yue Cao, Silin Pan
Summary: Combining synthetic polymers and biomacromolecules can prevent thrombosis and intimal hyperplasia in small-diameter vascular grafts (SDVGs). In this study, a bi-layered scaffold using electrospinning poly (L)-lactic acid (PLLA) is developed to promote the capture and differentiation of endothelial colony-forming cells (ECFCs) and prevent thrombosis after implantation. The scaffold consists of an outer PLLA scaffold and an inner PLLA biomimetic membrane containing heparin, the peptide Gly-Gly-Gly-Arg-Glu-Asp-Val (GGG-REDV), and vascular endothelial growth factor (VEGF). The modified SDVGs showed improved hemocompatibility and successful capture and differentiation of ECFCs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
XiaoLing Cui, XiaoTan Wang, Jie Wen, Xiao Li, Nan Li, XuXiao Hao, BaoXiang Zhao, Xunwei Wu, JunYing Miao
Summary: This study demonstrates that the small chemical molecule CPP can effectively induce the differentiation of human dermal fibroblasts (HDFs) into vascular endothelial cells (VECs). The results show that CPP treatment leads to morphological changes in HDFs and upregulation of VEC markers. Additionally, in vitro and in vivo experiments confirm that CPP-induced HDFs possess the functions of VECs. The induction of VEC differentiation by CPP is associated with the increased expression of pro-angiogenic factors.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Yasaman Aghazadeh, Frankie Poon, Farida Sarangi, Frances T. M. Wong, Safwat T. Khan, Xuetao Sun, Rupal Hatkar, Brian J. Cox, Sara S. Nunes, M. Cristina Nostro
Summary: This study enhanced islet transplantation by using microvessels from adipose tissue, resulting in improved cell survival and glucose response in both human islets and hESC-derived pancreatic cells, ameliorating preexisting diabetes in three mouse models of T1D.
Article
Cell Biology
Fangyu Wang, Xuan Liu, Shaowen Li, Chen Zhao, Yumei Sun, Kuan Tian, Junbao Wang, Wei Li, Lichao Xu, Jing Jing, Juan Wang, Sylvia M. Evans, Zhiqiang Li, Ying Liu, Yan Zhou
Summary: The possibility of trans-differentiation of GBM cells into vascular cells is unlikely to occur during gliomagenesis, according to the study. The findings advance the understanding of cell lineage dynamics during gliomagenesis and have implications for targeted treatment of GBMs.
Review
Cell Biology
Jan K. Hennigs, Christiane Matuszcak, Martin Trepel, Jakob Koerbelin
Summary: Endothelial cells play crucial roles in facilitating physiological processes throughout the body, demonstrating tremendous cellular diversity. Different endothelial cell populations adapt to the specific needs of various organs and tissues, influencing vascular tone, blood coagulation, and cellular adhesion.
Article
Biotechnology & Applied Microbiology
Rahul Mallick, Erika Gurzeler, Pyry I. Toivanen, Tiina Nieminen, Seppo Yla-Herttuala
Summary: VEGF-B186R127S induces vascular growth in mouse heart by recruiting EPCs. It is a novel therapeutic agent for cardiac therapeutic angiogenesis to rescue myocardial tissue after an ischemic insult.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell Biology
Cyril Bouland, Pierre Philippart, Didier Dequanter, Florent Corrillon, Isabelle Loeb, Dominique Bron, Laurence Lagneaux, Nathalie Meuleman
Summary: Bone regeneration is a complex process relying on interactions between osteogenesis and angiogenesis. Tissue engineering provides strategies with regenerative cell sources, growth factors, and mechanical stimulation. Vascularization is crucial for bone formation and function, with studies showing synergy between mesenchymal stromal cells (MSCs) and endothelial progenitor cells (EPCs) in promoting regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Food Science & Technology
Xuejuan Xia, Gunannan Li, Qingli Dong, Jiong-Wei Wang, Jung Eun Kim
Summary: This review summarizes the impact of food and nutrients on endothelial progenitor cells (EPCs) and highlights the potential of EPCs as a biomarker for assessing the effectiveness of nutritional interventions in preventing cardiovascular diseases (CVDs). The findings indicate that a healthy diet and specific nutrients generally promote higher EPC numbers and enhanced EPC function, while an unhealthy diet impairs EPC function.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Nutrition & Dietetics
Hanxu Zhang, Shuang Zhou, Minxue Sun, Manqi Hua, Zhiyan Liu, Guangyan Mu, Zhe Wang, Qian Xiang, Yimin Cui
Summary: The review discusses the role of ferroptosis in endothelial cells in vascular diseases and explores potential therapeutic strategies for treating ferroptosis.
Review
Cardiac & Cardiovascular Systems
Emma L. Robinson, Andrew H. Baker, Mairi Brittan, Ian McCracken, G. Condorelli, C. Emanueli, P. K. Srivastava, C. Gaetano, T. Thum, M. Vanhaverbeke, C. Angione, S. Heymans, Y. Devaux, T. Pedrazzini, F. Martelli
Summary: This review discusses the challenges in studying the role and regulation of the non-coding transcriptome in cardiovascular disease, including poor annotation of the non-coding genome, determination of transcript cellular distribution, assessment of RNA processing, and identification of cell-type specific changes. It highlights similarities and differences in analyzing the non-coding and protein-coding transcriptomes, as well as the impact of lack of consensus and standardized methods on reproducibility of data.
CARDIOVASCULAR RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Kim van Kuijk, Jasper A. F. Demandt, Javier Perales-Paton, Thomas L. Theelen, Christoph Kuppe, Elke Marsch, Jenny de Bruijn, Han Jin, Marion J. Gijbels, Ljubica Matic, Barend M. E. Mees, Chris P. M. Reutelingsperger, Ulf Hedin, Erik A. L. Biessen, Peter Carmeliet, Andrew H. Baker, Rafael K. Kramann, Leon J. Schurgers, Julio Saez-Rodriguez, Judith C. Sluimer
Summary: Myeloid-specific PHD2cko and PHD3ko enhanced atherosclerotic plaque growth and macrophage apoptosis, while PHD2cko macrophages further activated fibroblast collagen secretion through paracrine SPP1 signaling via TREM2hi macrophages.
CARDIOVASCULAR RESEARCH
(2022)
Review
Cardiac & Cardiovascular Systems
Katherine M. Ross Stewart, Sophie L. Walker, Andrew H. Baker, Paul R. Riley, Mairi Brittan
Summary: While humans cannot regenerate their hearts after injury, zebrafish have the ability to fully recover from cardiac insults. The regeneration process in zebrafish involves multiple tissue responses and gene regulatory patterns, with cardiomyocyte regeneration depending on neovascularization and lymphangiogenesis. Studies in zebrafish have provided insights into the mechanisms of cardiovascular repair and regeneration, which can inform strategies for treating human patients with cardiovascular disease.
CARDIOVASCULAR RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Julie Rodor, Shiau-Haln Chen, Jessica P. Scanlon, Joao P. Monteiro, Axelle Caudrillier, Sweta Sweta, Katherine Ross Stewart, Alena Shmakova, Ross Dobie, Beth E. P. Henderson, Kevin Stewart, Patrick W. F. Hadoke, Mark Southwood, Stephen D. Moore, Paul D. Upton, Nick W. Morrell, Ziwen Li, Stephen Y. Chan, Adam Handen, Robert Lafyatis, Laura P. M. H. de Rooij, Neil C. Henderson, Peter Carmeliet, Ana-Mishel Spiroski, Mairi Brittan, Andrew H. Baker
Summary: This study characterizes the dynamics of endothelial cells in pulmonary arterial hypertension (PAH) at a single-cell resolution. The findings reveal transcriptomic changes in PAH-induced endothelial cells and identify potential therapeutic targets.
CARDIOVASCULAR RESEARCH
(2022)
Review
Cardiac & Cardiovascular Systems
Lisa M. Becker, Shiau-Haln Chen, Julie Rodor, Laura P. M. H. de Rooij, Andrew H. Baker, Peter Carmeliet
Summary: Endothelial cells play a crucial role in the human body but their heterogeneity poses challenges in therapy. Recent studies have revealed the diverse functions of endothelial cells and their roles in diseases, and proposed strategies to deal with this heterogeneity.
CARDIOVASCULAR RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Ian R. McCracken, Ross Dobie, Matthew Bennett, Rainha Passi, Abdelaziz Beqqali, Neil C. Henderson, Joanne C. Mountford, Paul R. Riley, Chris P. Ponting, Nicola Smart, Mairi Brittan, Andrew H. Baker
Summary: The study aims to characterize the transcriptome of human foetal cardiac endothelium using single-cell RNA sequencing (scRNA-seq) to provide insights into cellular heterogeneity and transcriptional dynamics underlying endothelial specification. Results reveal distinct endothelial cell populations, endocardial contribution to coronary vasculature, and arterial specification of capillary endothelium. Transcriptional signatures defining endothelial subpopulations are largely conserved between human and mouse, with MECOM playing a role in maintaining arterial endothelial identity.
CARDIOVASCULAR RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Ziwen Li, Emmanouil G. Solomonidis, Bronwyn Berkeley, Michelle Nga Huen Tang, Katherine Ross Stewart, Daniel Perez-Vicencio, Ian R. McCracken, Ana Mishel Spiroski, Gillian A. Gray, Anna K. Barton, Stephanie L. Sellers, Paul R. Riley, Andrew H. Baker, Mairi Brittan
Summary: We present a high-resolution single-cell meta-atlas of healthy and injured coronary endothelial cells in the mouse and human heart, revealing a suite of novel targets with great potential to promote vascular regeneration, and providing a rich resource for therapeutic development.
CARDIOVASCULAR RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Ana-Mishel Spiroski, Ian R. McCracken, Adrian Thomson, Marlene Magalhaes-Pinto, Mukesh K. Lalwani, Kathryn J. Newton, Eileen Miller, Cecile Benezech, Patrick Hadoke, Mairi Brittan, Joanne C. Mountford, Abdelaziz Beqqali, Gillian A. Gray, Andrew H. Baker
Summary: This study investigated the tissue remodeling effects of human embryonic stem cell-derived endothelial cell product (hESC-ECP) therapy on infarcted heart and found that it reduces infarct size and preserves functional myocardium. By examining the interactions between cells and immune regulation, this research provides new therapeutic avenues for improving cardiac outcomes following myocardial infarction.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Kim van Kuijk, Ian R. McCracken, Renee J. H. A. Tillie, Sebastiaan E. J. Asselberghs, Dlzar A. Kheder, Stan Muitjens, Han Jin, Richard S. Taylor, Ruud Wichers Schreur, Christoph Kuppe, Ross Dobie, Prakesh Ramachandran, Marion J. Gijbels, Lieve Temmerman, Phoebe M. Kirkwoord, Joris Luyten, Yanming Li, Heidi Noels, Pieter Goossens, John R. Wilson-Kanamori, Leon J. Schurgers, Ying H. Shen, Barend M. E. Mees, Erik A. L. Biessen, Neil C. Henderson, Rafael Kramann, Andrew H. Baker, Judith C. Sluimer
Summary: This study established specific markers for adventitial fibroblasts and analyzed their heterogeneity in response to cardiovascular diseases and risk factors. The findings revealed the functional diversity and gene expression differences among different fibroblast clusters, which are associated with cardiovascular diseases. Therefore, this study is important for understanding cardiovascular diseases and developing targeted therapies.
CARDIOVASCULAR RESEARCH
(2023)
Letter
Cardiac & Cardiovascular Systems
Julie Rodor, Shiau-Haln Chen, Andrew H. Baker
CARDIOVASCULAR RESEARCH
(2023)
Article
Multidisciplinary Sciences
Ewan A. Ross, Lesley-Anne Turner, Hannah Donnelly, Anwer Saeed, Monica P. Tsimbouri, Karl V. Burgess, Gavin Blackburn, Vineetha Jayawarna, Yinbo Xiao, Mariana A. G. Oliva, Jennifer Willis, Jaspreet Bansal, Paul Reynolds, Julia A. Wells, Joanne Mountford, Massimo Vassalli, Nikolaj Gadegaard, Richard O. C. Oreffo, Manuel Salmeron-Sanchez, Matthew J. Dalby
Summary: This study reveals that nanotopography can regulate the immunomodulatory capacity of MSCs by reducing intracellular tension and increasing oxidative glycolysis, providing an effective approach for large-scale expansion of functional MSCs for therapeutic purposes.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
T. Singh, S. Joshi, M. N. Meah, N. B. Spath, G. Papanastasiou, L. E. Kershaw, A. H. Baker, M. R. Dweck, D. E. Newby, S. I. Semple
Summary: Manganese-enhanced magnetic resonance imaging can serve as a surrogate measure for myocardial calcium handling. The repeatability and reproducibility of this imaging method have been investigated in a study involving 68 participants. The results demonstrate excellent repeatability and reproducibility in healthy myocardium, but slightly lower consistency in patients with dilated cardiomyopathy.
SCIENTIFIC REPORTS
(2023)
Article
Cardiac & Cardiovascular Systems
Frederike Boos, James A. Oo, Timothy Warwick, Stefan Guenther, Judit Izquierdo Ponce, Melina Lopez, Diba Rafii, Giulia Buchmann, Minh Duc Pham, Zahraa S. Msheik, Tianfu Li, Sandra Seredinski, Shaza Haydar, Sepide Kashefiolasl, Karl H. Plate, Ruediger Behr, Matthias Mietsch, Jaya Krishnan, Soni S. Pullamsetti, Sofia-Iris Bibli, Rabea Hinkel, Andrew H. Baker, Reinier A. Boon, Marcel H. Schulz, Ilka Wittig, Francis J. Miller, Ralf P. Brandes, Matthias S. Leisegang
Summary: Long non-coding RNA LINC00607 is highly enriched in human endothelial cells and plays a regulatory role in angiogenesis by interacting with chromatin remodeler BRG1 to maintain ERG target gene transcription. Knockdown or knockout of LINC00607 attenuates VEGF-A-induced angiogenic sprouting and integration into vascular networks. Overexpression of LINC00607 restores normal endothelial function. These findings provide new insights into the role of lncRNAs in endothelial cells and their potential implications in cardiovascular disease.
BASIC RESEARCH IN CARDIOLOGY
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
T. Singh, S. Joshi, L. E. Kersahw, A. H. Baker, D. K. Dawson, M. R. Dweck, S. I. Semple, D. E. Newby
EUROPEAN HEART JOURNAL
(2022)
Meeting Abstract
Physiology
F. Boos, J. A. Oo, T. Warwick, S. Guenther, J. Izquierdo Ponce, G. Buchmann, T. Li, S. Seredinski, S. Haydar, S. Kashefiolasl, A. H. Baker, R. A. Boon, M. H. Schulz, I. Wittig, F. J. Miller, R. P. Brandes, M. S. Leisegang
