Article
Biochemistry & Molecular Biology
Shuai Yuan, Scott A. Hahn, Megan P. Miller, Subramaniam Sanker, Michael J. Calderon, Mara Sullivan, Atinuke M. Dosunmu-Ogunbi, Marco Fazzari, Yao Li, Michael Reynolds, Katherine C. Wood, Claudette M. St Croix, Donna Stolz, Eugenia Cifuentes-Pagano, Placido Navas, Sruti Shiva, Francisco J. Schopfer, Patrick J. Pagano, Adam C. Straub
Summary: The study demonstrates that CYB5R3 assists in NOX4-dependent H2O2 generation via CoQ, mitigating endothelial inflammatory activation. Knockout of CYB5R3 led to endothelial dysfunction in mice and enhanced VCAM-1 expression, which was alleviated by silencing NOX4. Overall, these findings suggest a regulatory role of CYB5R3 in endothelial inflammation through interaction with NOX4 and CoQ.
Review
Biochemistry & Molecular Biology
Martina Morchio, Emanuele Sher, David A. Collier, Daniel W. Lambert, Fiona M. Boissonade
Summary: Neuropathic pain is a highly prevalent condition that affects approximately 8% of the adult population in the UK. The underlying pathophysiology is complex and involves various processes, such as altered neuronal excitability, dysregulated intracellular signaling, and activation of immune and glial cells. Over the past 15 years, miRNAs have emerged as important regulators in neuropathic pain development, acting through the modulation of mRNA translation. Animal studies have identified several miRNAs involved in different stages of nociceptive pathways, but the translation of these findings to human subjects with neuropathic pain is still limited and dependent on the specific etiology. Further research using human tissue and liquid samples may provide valuable insights into miRNAs as diagnostic biomarkers or potential therapeutic targets for neuropathic pain.
Article
Biotechnology & Applied Microbiology
Yufu Xin, Xinrong Song, Qingye Ge
Summary: The study found that circSMEK1 promotes inflammation and microglia M1 polarization in NP by modulating the miR-216a-5p/TXNIP axis, providing a new molecular target for the future treatment of NP.
Article
Cell Biology
Chang-Ning Fu, Hui Wei, Wen-Shuang Gao, Sha-Sha Song, Shou-Wei Yue, Yu-Juan Qu
Summary: This study reveals that the AMPK-ERK-NOX4 pathway plays a crucial role in mediating obesity-induced increases in neuropathic pain. Activation of AMPK can inhibit the ERK-NOX4 pathway, oxidative stress, and inflammation caused by high-fat diets, ultimately reducing neuropathic pain in obese individuals.
Article
Biochemistry & Molecular Biology
Gesine Wack, Katharina Metzner, Miriam S. Kuth, Elena Wang, Anne Bresnick, Ralf P. Brandes, Katrin Schroeder, Ilka Wittig, Achim Schmidtko, Wiebke Kallenborn-Gerhardt
Summary: The study aimed to identify novel downstream interactors of Nox4 in the nociceptive system. Using proteomic approach, S100A4 was found to be up-regulated in peripheral nerves and dorsal root ganglia in a Nox4-dependent manner after injury. Deletion of S100A4 led to increased neuropathic pain hypersensitivity after peripheral nerve injury.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Immunology
Baolong Li, Jin Guo, Xiongyao Zhou, Weizhen Li, Ningning Wang, Rangjuan Cao, Shusen Cui
Summary: Neuropathic pain caused by somatosensory system injuries is challenging to treat. There is evidence suggesting that neuroinflammation and cell death play a role in the development of neuropathic pain. Pyroptosis, a form of programmed cell death related to inflammation, has the potential to enhance or sustain the inflammatory response. This review provides an overview of pyroptosis and its mechanisms, discussing its relevance in neuropathic pain and its therapeutic potential as a target.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Jian Li, Mouli Tian, Tong Hua, Haowei Wang, Mei Yang, Wenqian Li, Xiaoping Zhang, Hongbin Yuan
Summary: The impaired autophagy flux mainly occurs in astrocytes during the maintenance of neuropathic pain. Activation of autophagy can relieve pain, while inhibition of autophagy can aggravate pain and alter levels of neuroinflammation and reactive oxygen species. Further study shows that inhibition of autophagy can slow the induction of neuroinflammatory responses but increase their maintenance.
Article
Biochemistry & Molecular Biology
Wenyan Li, Yongjia Sheng, Jin Wang, Shasha Wu, Chenyang Han
Summary: This study reveals that the expression of miRNA-9a-5p is decreased in inflammatory bowel diseases, and it demonstrates the effects and mechanisms of miRNA-9a-5p in regulating the progression of these diseases. The study shows that miRNA-9a-5p targets NOX4 to suppress the production of ROS, which is crucial in mucosal barrier damage in inflammatory bowel diseases.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jyotsana Pandey, Jennifer L. Larson-Casey, Mallikarjun H. Patil, Rutwij Joshi, Chun-sun Jiang, Yong Zhou, Chao He, A. Brent Carter
Summary: Pulmonary fibrosis is a progressive lung disease characterized by macrophage activation. Asbestos-induced NOX4 expression in lung macrophages mediates fibrotic progression through mitochondrial ROS generation, while the mechanism of NOX4 localization to mitochondria is unknown. This study reveals that NOX4 localizes to the mitochondrial matrix via direct interaction with TIM23 in lung macrophages after asbestos exposure. The interaction between TIM23 and NOX4 is crucial for mitochondrial redox signaling and metabolic reprogramming in lung macrophages.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Immunology
Chongjie Yao, Jun Ren, Ruixin Huang, Cheng Tang, Yanbin Cheng, Zhizhen Lv, Lingjun Kong, Sitong Fang, Jiming Tao, Yangyang Fu, Qingguang Zhu, Min Fang
Summary: This study investigated the mechanisms by which manual therapy (MT) alleviates neuroinflammation and neuropathic pain caused by lumbar disk herniation (LDH). The researchers found that MT can improve neuroinflammation and reduce pain by regulating the expression of microRNAs (miRNAs) in the chronic compression of dorsal root ganglia (CCD) model.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Neurosciences
Tielong Liu, Ruixue Wang, Wenqiang Qi, Lei Jia, Ketao Ma, Junqiang Si, Jieting Yin, Yujia Zhao, Zhigang Dai, Jiangwen Yin
Summary: This study aimed to evaluate whether methyl ferulic acid alleviates neuropathic pain by inhibiting the expression of Nox4 and its induction of ferroptosis. The results showed that methyl ferulic acid can inhibit Nox4 protein expression, decrease ACSL4 expression, increase GPX4 expression, and reduce ROS, iron content, and abnormal mitochondrial number, thus alleviating neuropathic pain.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Medicine, Research & Experimental
Ning Liu, Guo-Xin Zhang, Chun-Hao Zhu, Xiao-Bing Lan, Miao-Miao Tian, Ping Zheng, Yu-Xiang Li, Jian-Qiang Yu
Summary: This study found that Echinacoside (ECH), an active compound of Cistanche deserticola Ma, could alleviate peripheral neuropathic pain by inhibiting microglia overactivation and inflammation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Neurosciences
Simin Tang, Huan Jing, Fuhu Song, Haicheng Huang, Wenjun Li, Guiling Xie, Jun Zhou
Summary: The study explores the role of microRNAs in neuropathic pain, specifically how miR-124 reverses NP by maintaining microglia quiescent, and miR-155 promotes NP by activating microglia. These findings provide insights for basic research and potential clinical treatments for neuropathic pain.
MOLECULAR NEUROBIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Alessandro Leuti, Marina Fava, Niccolo Pellegrini, Mauro Maccarrone
Summary: Inflammation and neuroinflammation play critical roles in generating neuropathic pain in chronic diseases, with specialized pro-resolving mediators emerging as crucial in regulating the inflammatory cascade and onset of chronic inflammation and pain. Accumulating evidence suggests a synergy between these mediators and other signaling pathways, implying a more intricate relationship between inflammation and pain perception than initially anticipated.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Yuanyuan Li, Chengyu Yin, Boyu Liu, Huimin Nie, Jie Wang, Danyi Zeng, Ruixiang Chen, Xiaofen He, Junfan Fang, Junying Du, Yi Liang, Yongliang Jiang, Jianqiao Fang, Boyi Liu
Summary: The study established a rat PIPN model and found astrocyte and microglia activation in the spinal cord dorsal horn, as well as identified immune/inflammatory responses and neurotrophin signaling pathways as important mechanisms in PIPN. This research may help in identifying potential genes or pathways for PIPN therapeutics.
JOURNAL OF NEUROINFLAMMATION
(2021)