Article
Biotechnology & Applied Microbiology
Kaihong Bai, Xiaoli Xu, Xudong Wang, Yao Li, Chengxuan Yu, Na Jiang, Jianqiang Li, Laixin Luo
Summary: This study used RNA-seq technology to investigate the mechanism of VBNC state induced by copper ion in Xcc. The results showed significant changes in gene expression and enriched metabolism-related pathways in different VBNC stages. Genes associated with cell motility were down-regulated, while pathogenicity related genes were up-regulated. This study not only summarized the related pathways of VBNC state, but also provided new ideas for studying gene expression profiles in different survival states under stress.
Article
Immunology
Qian Zhao, Fang Liu, Cong Song, Tingting Zhai, Ziwei He, Limei Ma, Xuemeng Zhao, Zhenhua Jia, Shuishan Song
Summary: In cabbage and Arabidopsis, low concentration of DSF can enhance plant immunity against Xcc. DSF treatment triggered an increased burst of ROS, up-regulated the expression of RBOHD and RBOHF, and increased the activity of antioxidase POD. Transcriptome and metabolome analysis showed that the plant hormone jasmonic acid (JA) signaling pathway was involved in DSF-primed resistance to Xcc.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Plant Sciences
Biswajit Samal, Subhadeep Chatterjee
Summary: The study revealed the important role of quorum sensing in phytopathogens, facilitating Xanthomonas campestris colonization in cabbage leaves and triggering chlorosis, promoting systemic infection. The quorum sensing response in the Xanthomonas group maximizes population fitness, triggering host chlorophagy at specific stages and establishing systemic infection.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Lu Lu, Sokrat G. Monakhos, Yong Pyo Lim, So Young Yi
Summary: The study identified significant differences in the expression of defense-related genes and antioxidant activity between black rot disease-resistant cabbage lines and susceptible lines, indicating a potential role for Xcc-induced reactive oxygen species in black rot disease development.
Article
Plant Sciences
Pari Madloo, Margarita Lema, Victor Manuel Rodriguez, Pilar Soengas
Summary: The study found that GHPs inhibit the growth of Xcc, leading to the need for Xcc to stop cell division to repair DNA and cell envelope. Furthermore, GHPs also alter the energy metabolism of Xcc and induce detoxification mechanisms.
Article
Microbiology
Filip Gazdik, Samuel Magnus, Steven J. Roberts, Rafal Baranski, Jana Cechova, Robert Pokluda, Ales Eichmeier, Dariusz Grzebelus, Miroslav Baranek
Summary: This study aimed to monitor the persistence of Xanthomonas campestris pv. campestris (Xcc) in field soil in central Europe, using different PCR assays. It was found that Xcc can persist in soil for up to two years, and the nested real-time PCR method showed higher sensitivity in detecting Xcc in soil compared to conventional PCR.
Article
Materials Science, Multidisciplinary
Jakub Pecenka, Zuzana Bytesnikova, Tomas Kiss, Eliska Penazova, Miroslav Baranek, Ales Eichmeier, Dorota Tekielska, Lukas Richtera, Robert Pokluda, Vojtech Adam
Summary: The study found that silver nanoparticles of different sizes and concentrations have inhibitory effects on Xcc bacterial infection, while hot water treatment can also inhibit the bacteria, however, significantly reducing the germination rate of seeds compared to AgNP treatment.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Plant Sciences
Li Yang, Chuanji Zhao, Zetao Bai, Lingli Yang, M. Eric Schranz, Shengyi Liu, Klaas Bouwmeester
Summary: This study compared two Brassica napus lines with different resistance levels to black rot disease and evaluated the differential gene expression during infection. The resistant line showed a gradual increase in differentially expressed genes (DEGs), while the susceptible line displayed a steady amount of DEGs. Analysis of gene co-expression networks identified multiple defense-related genes and enriched pathways associated with defense.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Adamantia Varympopi, Anastasia Dimopoulou, Dimitris Papafotis, Pavlos Avramidis, Ioannis Sarris, Theodora Karamanidou, Alexandra Kaldeli Kerou, Afroditi Vlachou, Eleftherios Vellis, Andreas Giannopoulos, Kosmas Haralampidis, Ioannis Theologidis, Dimitris G. Hatzinikolaou, Alexander Tsouknidas, Nicholas Skandalis
Summary: This study tested the ability of two copper nanoparticle products to control bacterial spot disease in tomatoes, and found that both nanoparticles effectively suppressed the growth of the causal agent and exhibited superior bioavailability compared to conventional formulations. This is an important report linking copper bioavailability to nanoparticle efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Jia-Bao Li, Lan-Tu Xiong, Yan-Rong Lu, Yu-Qing Zhang, Xiao-Li Xu, Hai-Hong Wang, Xin Deng, Xu-Hong Hu, Zi-Ning Cui
Summary: Cruciferous black rot, caused by Xcc infection, is a widespread disease globally. Excessive use of bactericide leads to bacterial resistance. This study discovered that compound SPF-9 inhibits virulence and pathogenicity of Xcc, showing potential as a novel T3SS inhibitor.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Carmen Vega-alvarez, Marta Francisco, Pilar Soengas
Summary: The study reveals that Xcc infection negatively impacts the biomass and photosynthesis of young B. oleracea plants, but adult plants show better immune response with limited effects on leaves and marketable products biomass.
Article
Microbiology
Kaihong Bai, Huayu Yan, Xing Chen, Qingyang Lyu, Na Jiang, Jianqiang Li, Laixin Luo
Summary: The alarmone ppGpp plays a crucial role in Xanthomonas campestris pv. campestris (Xcc) by influencing physiology, pathogenicity, and stress resistance, primarily through the mediation of SpoT. This study sheds new light on the significance of ppGpp in the biology of Xcc and may have broader implications for the survival and pathogenicity of other bacterial pathogens.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Valentina Furlanetto, Christina Divne
Summary: In this study, the first experimental structure of a complex between Xcc LolA and LolB was reported, revealing a stable complex in the absence of LP. The structural integrity of the LP-free complex was protected by specific protein-protein interactions that were different from those predicted to be involved in lipid binding. These findings identify structural determinants for Xcc LolA to dock with LolB and initiate LP transfer.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yaowanoot Promnuan, Saran Promsai, Wasu Pathom-aree, Sujinan Meelai
Summary: This study isolated 64 cultivable actinomycetes from the black dwarf honey bee in Thailand, with Streptomyces as the predominant genus. Some isolates displayed antimicrobial activity against plant pathogenic bacteria, particularly three Streptomyces isolates showed significant inhibitory effects against Xanthomonas campestris pv. campestris.
Article
Microbiology
Md Tabibul Islam, Bok-Rye Lee, Van Hien La, Dong-Won Bae, Woo-Jin Jung, Tae-Hwan Kim
Summary: The study investigated the response proteins of susceptible and resistant cultivars of Brassica napus to Xanthomonas campestris pv. campestris using gel-free quantitative proteomics. The results showed significant differences in protein expression between the two cultivars, with the resistant cultivar exhibiting more pronounced upregulation of redox-related and innate immunity-related proteins.
Article
Plant Sciences
Julien S. Luneau, Aude Cerutti, Brice Roux, Sebastien Carrere, Marie-Francoise Jardinaud, Antoine Gaillac, Carine Gris, Emmanuelle Lauber, Richard Berthome, Matthieu Arlat, Alice Boulanger, Laurent D. Noel
Summary: Analysis of Xanthomonas campestris pv. campestris transcriptome 3 days after entering cauliflower hydathodes revealed differential expression of 18% genes, with a striking repression of chemotaxis and motility functions. Although most virulence factors were not activated, the expression of 95 genes including those coding for type III secretion machinery was induced. Metabolic adaptations and limited stress responses were observed, with high-affinity phosphate transport confirmed to be important for bacterial fitness inside hydathodes.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zoe E. Dubrow, Sara C. D. Carpenter, Morgan E. Carter, Ayress Grinage, Carine Gris, Emmanuelle Lauber, Jules Butchachas, Jonathan M. Jacobs, Christine D. Smart, Matthew A. Tancos, Laurent D. Noel, Adam J. Bogdanove
Summary: In this study, the genomes of a collection of X. campestris strains were fully sequenced using multiplexed long-read technology. The results revealed the genomic relationships among pathovars and candidate genes for host- and tissue-specificity. The study also identified AvrAC and cbsA genes as being associated with host range and tissue specificity of X. campestris.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Biology
Julien S. Luneau, Mael Baudin, Thomas Quiroz Monnens, Sebastien Carrere, Olivier Bouchez, Marie-Francoise Jardinaud, Carine Gris, Jonas Francois, Jayashree Ray, Babil Torralba, Matthieu Arlat, Jennifer D. Lewis, Emmanuelle Lauber, Adam M. Deutschbauer, Laurent D. Noel, Alice Boulanger
Summary: Plant diseases pose a significant threat to food production, but our understanding of pathogen survival mechanisms during host colonization is limited. By conducting a genome-wide screen, we identified key genes involved in the adaptation and fitness of the Xcc pathogen in the cauliflower host plant. The study provides insights into the social behaviors and regulatory mechanisms of Xcc during early infection stages.
Article
Plant Sciences
Marie-Francoise Jardinaud, Sebastien Carrere, Benjamin Gourion, Pascal Gamas
Summary: Symbiotic nitrogen fixation plays a key role in reducing the negative impact of nitrogen fertilizers in agroecosystems. The efficiency of this process is influenced by the combination of bacterial and plant genotypes, but the mechanisms behind differences in the efficiency of rhizobium strains are not well known.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Kaichi Huang, Mojtaba Jahani, Jerome Gouzy, Alexandra Legendre, Sebastien Carrere, Jose Miguel Lazaro-Guevara, Eric Gerardo Gonzalez Segovia, Marco Todesco, Baptiste Mayjonade, Nathalie Rodde, Stephane Cauet, Isabelle Dufau, S. Evan Staton, Nicolas Pouilly, Marie-Claude Boniface, Camille Tapy, Brigitte Mangin, Alexandra Duhnen, Veronique Gautier, Charles Poncet, Cecile Donnadieu, Tali Mandel, Sariel Hubner, John M. Burke, Sonia Vautrin, Arnaud Bellec, Gregory L. Owens, Nicolas Langlade, Stephane Munos, Loren H. Rieseberg
Summary: This study analyzed the impacts of wild introgressions in cultivated sunflower on the genomic and phenotypic level, as well as the consequences of linkage drag. It was found that introgressions had negative effects on yield and quality traits, and high-frequency introgressions had larger effects than low-frequency ones.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Sebastien Carrere, Baptiste Mayjonade, David Lalanne, Sylvain Gaillard, Jerome Verdier, Nicolas W. G. Chen
Summary: Flageolet bean, originating from France, has a high-quality genome sequence that can be used for further genomic and genetic studies on common bean and legumes in general. This is the first whole-genome sequence of a common bean accession from Europe.
Article
Biochemistry & Molecular Biology
Francesca Marzorati, Rossana Rossi, Letizia Bernardo, Pierluigi Mauri, Dario Di Silvestre, Emmanuelle Lauber, Laurent D. Noel, Irene Murgia, Piero Morandini
Summary: This study investigates the early leaf responses of Arabidopsis thaliana to Pseudomonas simiae WCS417 exposure and the potential involvement of formate dehydrogenase (FDH) in these responses. The results reveal that chloroplasts, particularly components of the photosystems PSI and PSII, as well as members of the glutathione S-transferase family, are among the early targets of the metabolic changes induced by WCS417.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Plant Sciences
Cyril Libourel, Jean Keller, Lukas Brichet, Anne-Claire Cazale, Sebastien Carrere, Tatiana Vernie, Jean-Malo Couzigou, Caroline Callot, Isabelle Dufau, Stephane Cauet, William Marande, Tabatha Bulach, Amandine Suin, Catherine Masson-Boivin, Philippe Remigi, Pierre-Marc Delaux, Delphine Capela
Summary: By comparing the symbiotic transcriptomic responses of nine host plants, we reconstructed the ancestral and intermediate steps of root nodule symbiosis (RNS), finding that bacterial signals, nodule infection, nodule organogenesis, and nitrogen fixation were ancestral, while the release of symbiosomes was associated with recently evolved genes encoding small proteins in each lineage.
Article
Plant Sciences
Y. Pecrix, E. Sallet, S. Moreau, O. Bouchez, S. Carrere, J. Gouzy, M-F Jardinaud, P. Gamas
Summary: Plant epigenetic regulations play important roles in transposable element silencing, developmental processes, and responses to the environment. Specifically, DNA methylation modifications are involved in these processes. The development of root nodules, which are important for nitrogen fixation, is a complex process with various regulatory mechanisms. In this study, laser capture microdissection and RNA-sequencing techniques were used to identify genes activated in the nodule differentiation and nitrogen fixation zones. MtDME, which is upregulated in the differentiation zone, was found to be required for nodule development. Additionally, differentially methylated regions were identified in the nodule. CRISPR-Cas9 mutagenesis of MtDRM2 further demonstrated the importance of RdDM in CHH hypermethylation and nodule development. A model of DNA methylation dynamics during nodule development was proposed.
Article
Plant Sciences
Marie-Francoise Jardinaud, Justine Fromentin, Marie-Christine Auriac, Sandra Moreau, Yann Pecrix, Ludivine Taconnat, Ludovic Cottret, Gregoire Aubert, Sandrine Balzergue, Judith Burstin, Sebastien Carrere, Pascal Gamas
Summary: The study reveals that nitrogen-fixing symbiosis recruited two homologous root transcription factors for nodule development, providing a key insight into the coordination of plant and bacterial cell differentiation during this process.