Review
Cell Biology
Peiyuan Huang, Zhihao Zhao, Liting Duan
Summary: Dynamic protein-protein interactions are crucial for cell functioning, and homo-interactions are widely used to activate intracellular signaling pathways. Optogenetic tools based on light-sensitive proteins offer unprecedented specificity and spatiotemporal precision in dissecting complex signaling networks. This review focuses on recent progress in developing optogenetic tools for light-inducible protein-protein homo-interactions and their applications in optical activation of signaling pathways.
NEURAL REGENERATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Dan Ma, Ping Liu, Chujiao Hu, Zhen Zhou, Ping Wang, Yan Wang, Yaming Zhang, Yunsheng Ran, Pinghao Li, Jiangyuan Zhao, Jishi Wang, Chengliang Zhang, Lei Tang
Summary: This study revealed the important role of intracellular angiopoietin-1 (ANG-1) overexpression in drug resistance of chronic myeloid leukemia (CML), which promotes CML cell resistance through activation of the JAK/STAT pathway and enhancement of STAT5a phosphorylation. Therefore, intracellular ANG-1 may serve as a potential strategy for overcoming CML resistance.
Article
Multidisciplinary Sciences
Marco A. Alfonzo-Mendez, Kem A. Sochacki, Marie-Paule Strub, Justin W. Taraska
Summary: The study reveals the interaction between growth factor signaling and cell adhesion, and highlights the role of flat clathrin lattices in enhancing signal transduction.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Deisy Perdomo, Jose Bubis
Summary: The study identified the presence of Src family tyrosine kinases in dark-adapted retinas but found that the purified kinase was unable to use transducin as a substrate. This suggests that the specific cellular role of this retinal Src family member remains unknown.
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
(2021)
Review
Orthopedics
M. N. Ferrao Blanco, H. Domenech Garcia, L. Legeai-Mallet, G. J. V. M. van Osch
Summary: Osteoarthritis (OA) is a major global health issue characterized by joint pain and inflammation. Tyrosine kinases have been associated with chondrocyte hypertrophy, highlighting their potential as therapeutic targets for OA.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Biochemistry & Molecular Biology
Jianmei Hou, Elizabeth Nguyen, Minglyanna Surudoi, Michael J. Roy, Onisha Patel, Isabelle S. Lucet, Xiuquan Ma, Roger J. Daly
Summary: This article investigates the regulation and role of pseudokinase scaffolds PEAK1 and PEAK2 in cell signaling. The study finds that PEAK3, the third family member, also plays a crucial role in promoting cell proliferation, migration, and invasion by interacting with various proteins.
Review
Neurosciences
Kelly G. Sullivan, Greg J. Bashaw
Summary: Friedrich Bonhoeffer made significant contributions to the study of axon guidance in the developing nervous system by uncovering key cellular and molecular mechanisms and cloning important repulsive axon guidance cues. This review highlights his major contributions and ongoing investigations into the regulation of axon repulsion. The post-translational regulation of ephrin and slit ligands and their respective receptors is discussed, with a focus on endocytic trafficking events and receptor signaling. The article aims to identify outstanding questions for future research. Published by Elsevier Ltd. as part of a Special Issue entitled "Prof. Friedrich Bonhoeffer". All rights reserved.
Review
Plant Sciences
Stephen J. Bordeleau, Laura Canales E. Sanchez, Daphne R. Goring
Summary: Successful fertilization of a flowering plant requires cell-to-cell communication between pollen grain and pistil, where ligand-receptor kinase signaling plays a crucial role. While the later stages of pollen-pistil interactions have been well studied in Arabidopsis, the earlier stages remain less clear. Recent studies have implicated predicted receptor kinases in regulating pollen hydration and growth in the upper regions of the pistil. However, many questions regarding their specific functions remain unanswered.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Michael D. Paul, Kalina Hristova
Summary: This study proposes a provocative hypothesis that active, ligand-bound RTK dimers from unrelated subfamilies can associate into heterooligomers with novel signaling properties, supported by quantitative FRET study. Direct interactions between EGFR and VEGFR2 are observed in the absence of ligand and in the presence of both ligands, suggesting a novel mechanism for signal diversification.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Krisztina Futosi, Boglarka Bajza, Dorottya Deli, Andras Erdelyi, Simon Tusnady, Attila Mocsai
Summary: Tyrosine kinases play crucial roles in various biological processes and are targeted in the treatment of malignant diseases and immune-mediated disorders. We have developed a rapid in vivo assay using flow cytometry to analyze the effect of oral tyrosine kinase inhibitors on basal tyrosine phosphorylation of circulating mouse neutrophils. The assay allows for efficient evaluation of the in vivo efficacy of these inhibitors without sacrificing the mice. Results showed that the basal tyrosine phosphorylation of neutrophils was significantly reduced by the Abl/Src-family inhibitor dasatinib, and dose-response and kinetic studies revealed the optimal dosage and time for maximal reduction of tyrosine phosphorylation. This assay can serve as an alternative approach for assessing the in vivo effect of tyrosine kinase inhibitors.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Cell Biology
Andra Fortner, Alexandra Chera, Antoanela Tanca, Octavian Bucur
Summary: C-terminal Src kinase (CSK) plays a crucial role in regulating critical cellular decisions, mainly through negative regulation of the SRC family of tyrosine kinases (SFKs) to promote apoptosis. Abnormal functioning of CSK and activation of SFKs are associated with diseases such as cancer, cardiovascular, and neurological disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Sandra Berndt, Ines Liebscher
Summary: SFKs are crucial regulators of cell proliferation, differentiation, and survival, with their expression strongly linked to cancer development and tumor progression. The regulation of SFKs through GPCR-mediated pathways is complex and may involve direct protein interactions or allosteric regulation by arrestins and G proteins. The potential direct interaction between GPCRs and SFKs could lead to a novel mechanism of SFK signaling and identification of new GPCR-SFK interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
So Jeong Paik, Dong Joon Kim, Sung Keun Jung
Summary: Skin is directly exposed to external factors like ultraviolet and particulate matter, which can cause chronic skin diseases. Phytochemicals have the potential to prevent and treat skin diseases by regulating signaling pathways. Further research is needed to validate the clinical potential of phytochemicals in skin disease prevention and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Yuhong Zhai, Jun Yang, Jing Zhang, Jian Yang, Qi Li, Tao Zheng
Summary: SFKs play a crucial role in the development of various cardiovascular diseases by regulating signaling pathways to maintain cardiovascular homeostasis. They have the potential to serve as diagnostic biomarkers and therapeutic targets, paving the way for further clinical applications.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Medicine, General & Internal
Danny Alon, Yossi Paitan, Eyal Robinson, Nirit Ganor, Julia Lipovetsky, Rinat Yerushalmi, Cyrille J. Cohen, Annat Raiter
Summary: CD45, a predominant transmembrane tyrosine phosphatase in leukocytes, is crucial for T cell receptor signaling and activation, making it a potential therapeutic target. The immune modulating therapeutic peptide C24D can reset immune system functionality via the CD45 signaling pathway, showing potential immune reactivating effects on PBMCs from TNBC and COVID-19 patients. This study suggests CD45 as a potential therapeutic target due to its central role in the immune system.
FRONTIERS IN MEDICINE
(2021)