Article
Chemistry, Analytical
Lanyu Liu, Qian Liu, Yan Wang, Yu Yuan, Feifan Xie
Summary: This study aimed to evaluate different combinations of polyanion-metal ion based solution system for phospholipids removal and elimination of matrix effects in human plasma. The optimized ZrOCl2-Ludox/DSS systems provided efficient phospholipids removal and adequate analyte recovery, and they also correctly eliminated ion suppression or enhancement of the non-polar and polar drugs.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Hana Bavlovic Piskackova, Elisabeth Leere Oiestad, Nela Vanova, Julia Lengvarska, Petra Sterbova-Kovarikova, Stig Pedersen-Bjergaard
Summary: In this study, Electromembrane extraction (EME) of polar zwitterionic drugs anthracyclines from rabbit plasma was investigated and compared with conventional sample pretreatment techniques. The EME showed high extraction recovery and excellent sample clean-up, proving to be a simple, rapid, and repeatable technique for extraction of ANT from plasma.
Article
Biochemical Research Methods
El zbieta Gniazdowska, Joanna Giebultowicz, Piotr J. Rudzki
Summary: Mass spectrometry coupled with liquid chromatography is a valuable tool for drug development and personalised drug therapy. The matrix effect is caused by enhancing or suppressing the analyte signal intensity by the interfering compounds of biological fluids. The matrix effect may influence the reliability of the quantitative results, thus its evaluation is critical in bioanalytical method validation.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
John Quinones, Rommy Diaz, Jorge F. Beltran, Lidiana Velazquez, David Cancino, Erwin Munoz, Patricio Dantagnan, Adrian Hernandez, Nestor Sepulveda, Jorge G. Farias
Summary: This study analyzed the effects of different feeds on the lipid composition of juvenile Oncorhynchus mykiss muscle and found that the inclusion of Brassica napus oil significantly increased certain lipid levels, while Durvillaea antarctica meal had a less significant impact on lipid variation.
Article
Chemistry, Analytical
Andisiwe Malo, Tracy Kellermann, Elisa H. Ignatius, Kelly E. Dooley, Rodney Dawson, Anton Joubert, Jennifer Norman, Sandra Castel, Lubbe Wiesner
Summary: A method for the extraction and quantification of pretomanid in human plasma using LC-MS/MS was developed and validated. The method showed good performance in accuracy, precision, selectivity, sensitivity, recovery and stability, and was specific for pretomanid.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Multidisciplinary
Mohammad H. Alyami, Amal M. Fakhry, Nancy M. El Halfawy, Soliman M. Toto, Nada K. Sedky, Heba A. Yassin, Sherif Ashraf Fahmy, Fatma A. Mokhtar
Summary: In this study, silver nanoparticles (RME-AgNPs) were synthesized using Retama monosperma extract (RME), and their antibacterial activity was evaluated. The chemical composition of RME was identified using LC-ESI/MS/MS, and 21 phenolic metabolites were identified. The RME-AgNPs exhibited good antibacterial activity against both Gram-negative and Gram-positive bacteria, causing severe damage to the bacterial cell wall. The study also identified potential molecular targets and signaling pathways involved in the antibacterial activity of RME-AgNPs.
Article
Food Science & Technology
Peng Zhao, Jiangbing Qiu, Aifeng Li, Guowang Yan, Min Li, Ying Ji
Summary: This study found that the extraction ratio has a significant impact on the accuracy of BMAA analyzed in different biological samples, recommending the best extraction ratios for different matrices. The comparison enhances the performance of solid-phase extraction (SPE) purification protocol and the accuracy of LC-MS/MS analysis of BMAA.
Article
Chemistry, Multidisciplinary
Chang Shu, Teng-Fei Li, Duo Li, Zhong-Qiu Li, Xing-Hua Xia
Summary: Pomalidomide, an IMiD approved by the FDA for multiple myeloma treatment, was quantified in human plasma using a validated LC-MS/MS method. The method was successfully applied to clinical pharmacokinetic studies with positive results.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Analytical
Jakub Maculewicz, Piotr Stepnowski, Joanna Dolzonek, Anna Bialk-Bielinska
Summary: Imidazolium ionic liquids (ILs) are increasingly being used, and there is evidence of their presence in the environment. Due to their potential for bio-concentration, it is necessary to monitor the presence of these compounds in organisms from potentially contaminated waters. This study developed a novel analytical procedure for the determination of imidazolium ILs in mussel tissues using a combination of extraction techniques and LC-MS/MS analysis.
Article
Chemistry, Applied
Rahul D. Damale, Anirban Dutta, Nasiruddin Shaikh, Anita Pardeshi, Raviraj Shinde, K. Dhinesh Babu, Nilesh N. Gaikwad, Kaushik Banerjee
Summary: The study used GC-MS/MS and LC-MS/MS to investigate the matrix effects (MEs) of four different Indian pomegranate cultivar extracts on multi-class pesticides. The results showed that each cultivar had unique MEs for each pesticide, and most pesticides exhibited significant variations in recovery rates.
Article
Microbiology
Xiaofei Wang, Xiaojuan Wang, Tongkai Cai, Yulin Qin, Ling Li, Yuanying Jiang, Bing Han, Yongbing Cao
Summary: In this study, a simple and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed to quantify the intracellular fluconazole (FLC) content in the opportunistic human fungal pathogen Candida albicans. The method was found to be accurate, efficient, and reproducible, and was successfully validated in 36 clinical isolated strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
So-Hee Kim, Yoon-Hee Lee, Mun-Ju Jeong, Da-Yeong Gwon, Ji-Ho Lee, Yongho Shin, Hoon Choi
Summary: The matrix effect refers to the change in the analytical signal caused by the matrix in which the sample is contained, as well as the impurities that are co-eluted with the target analyte. In crop sample analysis using LC-MS/MS, the matrix effect can affect the quantification results. Chinese chives are likely to exhibit a strong matrix effect when co-extracted with bifenthrin and butachlor due to the presence of phytochemicals and chlorophyll. A novel analytical method was developed to reduce the matrix effects of bifenthrin and butachlor to a negligible level in Chinese chives.
Article
Biochemistry & Molecular Biology
Marjeta Mencin, Maja Mikulic-Petkovsek, Robert Veberic, Petra Terpinc
Summary: A solid-phase extraction (SPE) technique was developed and optimised for isolation and concentration of extractable and bound phenolic acids from germinated spelt seeds, for analysis by liquid chromatography-mass spectrometry. The study found that this method has significant advantages over traditional liquid-liquid extraction in terms of higher extraction efficiencies and can be used for quantitative analysis of extractable and bound phenolic acids.
Article
Biochemical Research Methods
Mihai Victor Curtasu, Natalja P. Norskov
Summary: The present work aims to develop a high-yield extraction procedure combined with a robust, sensitive, and fast LC-MS/MS method for quantifying flavonoids and salicylic acid in Willow bark. The research investigates the effect of freeze and oven-drying procedures and five extraction solvents on the yield of individual flavonoids and salicylic acid. The freeze-drying procedure is the best for preserving monomeric and polymeric flavan-3-ols, while the best extraction solvent is methanol acidified with 1% hydrochloric acid.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Rico Schwarz, Anna Richter, Elisabeth R. D. Ito, Hugo Murua Escobar, Christian Junghanss, Burkhard Hinz
Summary: Silmitasertib is a drug currently being investigated in clinical trials for the treatment of various cancers. It inhibits CK2 protein kinase, which has proliferation-promoting and anti-apoptotic effects on cancer cells. Researchers have developed an LC-MS/MS method for quantifying silmitasertib in human plasma.
