Article
Biochemistry & Molecular Biology
Chiara Lavarello, Sebastiano Barco, Martina Bartolucci, Isabella Panfoli, Emanuele Magi, Gino Tripodi, Andrea Petretto, Giuliana Cangemi
Summary: LC-HRMS is the preferred method for untargeted metabolomic analysis, with established protocols to ensure accurate metabolite identification. The study successfully built an AMRT database using a commercial metabolite library, achieving level 1 identification of metabolites in pediatric plasma and urine samples, providing a foundation for future metabolomic studies in pediatric settings.
Article
Toxicology
Selina Hemmer, Lea Wagmann, Markus R. Meyer
Summary: The study utilized untargeted metabolomics to investigate the acute effects of amphetamine on the plasma and urinary metabolome in rats. It revealed a downregulation of amino acids in plasma samples after amphetamine exposure, and identified four new potential biomarkers in urine. The research highlights the need for further studies to elucidate altered metabolic pathways associated with acute amphetamine exposure.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Selina Hemmer, Lea Wagmann, Benedikt Pulver, Folker Westphal, Markus R. Meyer
Summary: This study investigated the effects of synthetic cathinones on in vitro and in vivo metabolomes, and identified relevant endogenous and exogenous biomarkers. The findings are important for establishing analytical screening procedures and understanding the physiological changes associated with cathinone abuse.
Article
Medical Laboratory Technology
Clarisse L. Torres, Fernanda B. Scalco, Maria Lucia C. de Oliveira, Roy W. A. Peake, Rafael Garrett
Summary: In this study, untargeted metabolomics using LC-HRMS was applied to identify potential biomarkers for MPS disorders. The study found significant differences in the metabolome of MPS patients compared to controls, which can be utilized for diagnosis and treatment monitoring of MPS.
CLINICA CHIMICA ACTA
(2023)
Article
Engineering, Environmental
Qiuguo Fu, Andreas Scheidegger, Endre Laczko, Juliane Hollender
Summary: The study investigated the toxicokinetic processes and metabolomics effects of the pharmaceutical diclofenac on the freshwater invertebrate Hyalella azteca. Results showed rapid uptake and formation of diclofenac taurine, with slower elimination compared to diclofenac. Metabolomics revealed inhibition of prostaglandin synthesis and impact on the carnitine shuttle pathway at environmentally relevant concentrations.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Microbiology
Wen-Nee Tan, Kashvintha Nagarajan, Vuanghao Lim, Juzaili Azizi, Kooi-Yeong Khaw, Woei-Yenn Tong, Chean-Ring Leong, Nelson Jeng-Yeou Chear
Summary: Endophytic fungi Diaporthe fraxini can produce bioactive metabolites with potential pharmacological activities. This study used metabolomics to investigate the variations in metabolomes of D. fraxini grown in different culture media. The supplemented culture medium stimulated the production of metabolites not found in the normal culture medium, and the fungal extract showed antioxidant activity.
Review
Chemistry, Analytical
Leticia Lacalle-Bergeron, David Izquierdo-Sandoval, Juan V. Sancho, Francisco J. Lopez, Felix Hernandez, Tania Portoles
Summary: There is a growing demand to increase knowledge about the quality of food and its relationship with health and disease. Untargeted metabolomics approaches are powerful tools for exploring metabolic changes, with potential in the food field. Advanced analytical techniques like high resolution mass spectrometry are necessary for covering a wide range of metabolites in complex samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Chloe Cloteau, Gaud Dervilly, Zied Kaabia, Florian Bagilet, Vivian Delcourt, Benoit Loup, Yann Guitton, Anne-Lise Royer, Fabrice Monteau, Patrice Garcia, Marie-Agnes Popot, Bruno Le Bizec, Ludovic Bailly-Chouriberry
Summary: A non-targeted metabolomics approach was used to identify potential biomarkers associated with the abuse of Anabolic-Androgenic Steroids in animal competitions using equine urine samples. The model's performances and robustness were successfully preserved in a high-throughput liquid chromatography-selected reaction monitoring method. The transferability of the method to a second laboratory demonstrated its applicability in developing biomarker-based strategies for doping control.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Hani Habra, Maureen Kachman, Vasantha Padmanabhan, Charles Burant, Alla Karnovsky, Jennifer Meijer
Summary: This study developed a computational workflow for merging and harmonizing metabolomics data acquired under different LC-MS conditions. The researchers collected plasma metabolite profiles from two sets of maternal subjects using distinct instruments and LC-MS procedures. Their findings revealed significant metabolic changes in maternal plasma during pregnancy.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Nathaly Reyes-Garces, Ezel Boyaci, German A. Gomez-Rios, Mariola Olkowicz, Cian Monnin, Barbara Bojko, Dajana Vuckovic, Janusz Pawliszyn
Summary: This study evaluated SPME in combination with LC-HRMS as an analytical approach for untargeted brain analysis. The tested coating chemistries provided comparable brain tissue information, with some coatings showing higher relative standard deviations. Methanol was found to be essential for effective desorption of non-polar metabolites.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Food Science & Technology
Sebastian Imperiale, Ksenia Morozova, Giovanna Ferrentino, Matteo Scampicchio
Summary: This article reviews the analysis of milk and its components using liquid chromatography coupled with mass spectrometry (LC-MS). The major constituents of milk, including lipids, proteins, sugars, and minerals, are discussed.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Environmental Sciences
Sabrina Liesenfeld, Panagiotis Steliopoulos, Svenja Wenig, Vera Gottstein, Gerd Hamscher
Summary: The study analyzed urine samples from ALT-treated and non-medicated gilts, revealing glucuronide conjugates of ALT and its photo-isomerization product as the main metabolites in gilts. Non-targeted metabolomics analysis showed significant differences between the ALT-treated group and control group, with a hydroxylated ALT glucuronide identified as highly significant in the ALT-treated group. This study demonstrates the potential of LC-HRMS and metabolomics for detecting new environmental contaminants with high biological activity.
Article
Biochemical Research Methods
Maria Doppler, Christoph Bueschl, Florian Ertl, Jakob Woischitzschlaeger, Alexandra Parich, Rainer Schuhmacher
Summary: Phenylalanine is an important precursor for many secondary plant metabolites. Recent studies have shown that defense metabolites derived from phenylalanine are induced in wheat with fungal disease, and some of them are incorporated into the cell wall. A new approach was developed to screen phenylalanine-derived metabolites. The study also demonstrated the suitability of this method for typical metabolomics applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Roberto Stella, Eleonora Mastrorilli, Tobia Pretto, Alessandra Tata, Roberto Piro, Giuseppe Arcangeli, Giancarlo Biancotto
Summary: This study utilized a metabolomics approach to identify markers of freezing/thawing in European sea bass fish fillets and developed a model to classify fish samples according to their fresh or frozen/thawed condition with 100% accuracy, sensitivity, and specificity. EPA and DHA metabolites were found to be the most relevant predictors. The study suggests the potential development of a targeted method for providing robust species-independent tests in the seafood industry.
Article
Biochemistry & Molecular Biology
Marianna Martinello, Roberto Stella, Alessandra Baggio, Giancarlo Biancotto, Franco Mutinelli
Summary: Honey is a popular and expensive natural product that is commonly adulterated due to its high demand. Advanced non-targeted metabolomics techniques are used to detect honey adulteration, and potential markers of honey adulteration with sugar syrups have been identified.
