Article
Endocrinology & Metabolism
Jesper Krogh, Peter Plomgaard, Ruth Frikke-Schmidt, Sten Velschow, Jesper Johannesen, Linda Maria Hilsted, Malene Schroder, Ulla Feldt-Rasmussen
Summary: This study assessed the performance and safety of Fluispotter for the first time in healthy subjects. Fluispotter is a novel body-worn device with automated extraction, collection, and storage of venous dried blood spot samples. The majority of participants were able to successfully collect all the samples, demonstrating that Fluispotter is safe and feasible.
ENDOCRINE CONNECTIONS
(2023)
Article
Chemistry, Analytical
Ondrej Moravcik, Milos Dvorak, Pavel Kuban
Summary: A new set-up for fully autonomous and high-throughput capillary electrophoresis analysis of dried blood spot samples is presented. The set-up enables unmanned sample processing and analysis with tailor-programmed CE sequences, allowing for a throughput of 240 samples per day. The analytical protocol provides excellent precision and calibration curves with linear ranges and detection limits suitable for clinical purposes. The stability of the samples stored at laboratory temperature for up to 2 months is also demonstrated.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Isabel Ten-Domenech, Alvaro Solaz-Garcia, Inmaculada Lara-Canton, Alejandro Pinilla-Gonzalez, Anna Parra-Llorca, Maximo Vento, Guillermo Quintas, Julia Kuligowski
Summary: The GSH-to-GSSG ratio is critical for intracellular redox status. A new LC-MS method for measuring GSH and GSSG in DBS samples was developed, providing a convenient alternative to liquid blood samples. The levels of GSH-NEM and GSSG measured in DBS samples were comparable to those obtained from liquid blood samples in clinical scenarios.
Article
Chemistry, Analytical
Thomas Meikopoulos, Olga Begou, Georgios Theodoridis, Helen Gika
Summary: A new method for quantitative analysis of four ceramide species in dried blood samples was developed using LC-MS/MS. The Capitainer device was utilized to provide accurate sample volumes, allowing for accurate determinations. The study showed the potential application of at-home blood microsampling for health monitoring.
ANALYTICA CHIMICA ACTA
(2023)
Article
Environmental Sciences
Juana Maria Gonzalez-Rubio, Noelia Dominguez-Morueco, Susana Pedraza-Diaz, Ana Canas Portilla, Miguel Angel Lucena, Ana Rodriguez, Argelia Castano, Marta Esteban-Lopez
Summary: Human exposure to mercury can have serious health effects, especially in vulnerable groups such as children and fetuses. A novel method using dried blood spot (DBS) samples to analyze total mercury has shown good results in terms of precision, accuracy, and recovery. This method can be an excellent alternative for assessing mercury exposure in vulnerable groups in clinical settings.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Virology
Kathy Jackson, Rosemary Tekoaua, Xin Li, Stephen Locarnini
Summary: The study evaluated the performance of the Xpert HBV viral load assay in measuring Hepatitis B virus loads, showing good correlation with other high-throughput assays such as Roche and Abbott. The use of dried blood spots (DBS) also demonstrated potential for estimating viral loads accurately.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Biochemical Research Methods
Helena Beatriz Ferreira, Ines M. S. Guerra, Tania Melo, Hugo Rocha, Ana S. P. Moreira, Artur Paiva, M. Rosario Domingues
Summary: Dried blood spots (DBS) are being considered as an alternative method for blood collection in lipidomic and other omic analysis. While DBS are widely used in clinical settings for specific measurements, their application in lipidomic studies is still limited. The combination of DBS with lipidomic approaches has shown high sensitivity and specificity, making it a useful tool for biomarker identification.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Franklin Ducatez, Carine Pilon, Justine Ferey, Stephane Marret, Soumeya Bekri, Abdellah Tebani
Summary: This study evaluated the impact of different hematocrit levels and punching positions on the assessment of enzyme activity in dried blood spot (DBS) samples. The results showed that hematocrit, punching position, and the use of different sampling methods did not affect the assessment of enzyme activity, indicating the reliability of DBS in this test.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Marc Luginbuhl, Stefan Gaugler
Summary: This perspective discusses quantitative DBS analysis for anti-doping testing in an athletic population and argues that using only volumetric sampling may not be sufficient. It provides examples of HCT variations, discusses the whole blood to plasma ratio and HCT extraction bias, and presents options to correct for the HCT bias.
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Milena Rmandic, Ana Stajic, Jasna Jancic, Janko Samardzic, Nebojsa Jovic, Andelija Malenovic
Summary: A UHPLC-MS/MS method was developed and validated for the determination of zonisamide in DPS and DBS samples, showing its suitability for clinical application.
Article
Chemistry, Multidisciplinary
Ines Ait Belkacem, Noushine Mossadegh-keller, Penelope Bourgoin, Isabelle Arnoux, Marie Loosveld, Pierre-emmanuel Morange, Thibaut Markarian, Pierre Michelet, Jean Marc Busnel, Sandrine Roulland, Franck Galland, Fabrice Malergue
Summary: Blood cell analysis is crucial for biomedical research and healthcare, with dried blood spots offering a way to preserve cellular molecules. Optimized leucocyte elution techniques allow for analysis of recovered cells, and the new biomarker CD169 has the potential to differentiate patients.
Article
Chemistry, Analytical
Lucia Paniagua-Gonzalez, Elena Lendoiro, Esteban Otero-Anton, Manuel Lopez-Rivadulla, Ana de-Castro-Rios, Angelines Cruz
Summary: This study aimed to perform a clinical validation for tacrolimus and mycophenolic acid in both DBS and MitraTM VAMS, fulfilling both analytical and clinical acceptance criteria. VAMS showed lower concentrations compared to routine matrices but met the analytical requirements after correction.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Carmel E. Heiland, Magnus Ericsson, Anton Pohanka, Lena Ekstrom, Alexandre Marchand
Summary: This study investigated the use of dried blood spots (DBS) as a matrix for doping control and found optimal conditions for detecting prohibited erythropoietin receptor agonists (ERAs) and endogenous erythropoietin (EPO) from DBS. The results showed that 60 μl DBS could detect the main ERAs at concentrations close to the minimum required performance levels (MRPLs) set by WADA for serum/plasma samples. Storage temperature did not affect ERA detection. Compared with urine, DBS showed less variation in the detection of endogenous EPO.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Sara Amalie Solheim, Andreas Thomas, Thomas Kamm Ringsted, Mario Thevis, Andreas Breenfeldt Andersen, Henrik Holm-Sorensen, Nikolai B. Nordsborg, Jakob Morkeberg
Summary: Dried blood spot (DBS) testing is a fast, easy and minimally invasive method for collecting microvolumes of blood, and it has particular relevance in anti-doping control. This study demonstrates the feasibility of using alternative sampling sites and matrices for ephedrine analysis in DBS testing.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Yongtao Xiao, Ying Zhou, Kejun Zhou, Wei Cai
Summary: Using metabolomics profiling, this study identified potential biomarkers for biliary atresia in dried blood spots, providing a new approach for screening this condition.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Analytical
Elizabeth A. Crawford, Cemal Esen, Dietrich A. Volmer
ANALYTICAL CHEMISTRY
(2016)
Article
Chemistry, Analytical
Matthew C. Bernier, Frederick Li, Brian Musselman, Paul N. Newton, Facundo M. Fernandez
ANALYTICAL METHODS
(2016)
Article
Biochemical Research Methods
Andreas Jakob, Elizabeth A. Crawford, Juergen H. Gross
JOURNAL OF MASS SPECTROMETRY
(2016)
Review
Spectroscopy
Matthew J. Pavlovich, Brian Musselman, Adam B. Hall
MASS SPECTROMETRY REVIEWS
(2018)
Article
Medicine, Legal
Frederick Li, Joseph Tice, Brian D. Musselman, Adam B. Hall
Article
Chemistry, Analytical
Haiwei Gu, Zhengzheng Pan, Bowei Xi, Vincent Asiago, Brian Musselman, Daniel Raftery
ANALYTICA CHIMICA ACTA
(2011)
Article
Biochemical Research Methods
Elizabeth Crawford, Brian Musselman
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2012)
Article
Chemistry, Analytical
Yan-Jing Li, Zhen-Zhong Wang, Yu-An Bi, Gang Ding, Long-Sheng Sheng, Brian Musselman, Chen-Feng Zhang, Jun Chen, Wei Xiao
ANALYTICAL METHODS
(2013)
Article
Chemistry, Multidisciplinary
Mengxi Wu, Haoyang Wang, Guoqing Dong, Brian D. Musselman, Charles C. Liu, Yinlong Guo
CHINESE JOURNAL OF CHEMISTRY
(2015)
Article
Biochemical Research Methods
Joseph LaPointe, Brian Musselman, Teresa O'Neill, Jason R. E. Shepard
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2015)
Article
Biochemical Research Methods
Elena S. Chernetsova, Elizabeth A. Crawford, Alexander N. Shikov, Olga N. Pozharitskaya, Valery G. Makarov, Gertrud E. Morlock
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2012)
Article
Biochemical Research Methods
Kenyon M. Evans-Nguyen, Amanda Rivera, Jannelys Fontanez-Adames, Frederick Li, Brian Musselman
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2020)
Article
Chemistry, Analytical
Elizabeth A. Crawford, Stefanie Gerbig, Bernhard Spengler, Dietrich A. Volmer
ANALYTICA CHIMICA ACTA
(2017)