Article
Biochemistry & Molecular Biology
Elizabeth Pereida-Jaramillo, Gabriela B. Gomez-Gonzalez, Angeles Edith Espino-Saldana, Ataulfo Martinez-Torres
Summary: Radial glial cells play a central role in neuronal origin and placement, while glutamatergic signaling in adult cerebellum is crucial for motor coordination. Development of calcium activity in radial glia is related to morphological changes, as seen in transgenic zebrafish, where cells become larger and more elaborate over time. Spontaneous calcium events are scarce in early development, with calcium waves appearing later and being inhibited by gap junction coupling blockage. This study provides crucial insights into radial glia organization, morphology, and calcium signaling during development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Kevin Dorgans, Da Guo, Kiyoto Kurima, Jeff Wickens, Marylka Yoe Uusisaari
Summary: Adeno-associated viral (AAV) vectors are a powerful tool in modern neuroscience for gene transfer into the brain, allowing identification of specific neuronal populations and monitoring their activity. Researchers have successfully created an AAV vector for strong transfection of inferior olivary (IO) neurons and demonstrated the ability to monitor their activity.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Spectroscopy
Yu-Xu Tu, Natarajan Vijay, Han-Xiang Ko, Yuan-Pin Lo, Sivan Velmathi, Shu-Pao Wu
Summary: The new fluorescent probe ANP can rapidly and efficiently detect cysteine, and it can evaluate the distribution of cysteine in living cells and tissues.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biotechnology & Applied Microbiology
Laura Rode, Christian Baer, Sonja Gross, Axel Rossi, Nadja Meumann, Janika Viereck, Naisam Abbas, Ke Xiao, Isabelle Riedel, Anika Gietz, Karina Zimmer, Margarete Odenthal, Hildegard Buening, Thomas Thum
Summary: AAV2-THGTPAD and AAV2-NLPGSGD are promising novel tools for cardiac-directed gene therapy, outperforming AAV2 and AAV9 in terms of cardiac tissue tropism and transduction efficiency.
Article
Multidisciplinary Sciences
Antonio J. Santinha, Esther Klingler, Maria Kuhn, Rick Farouni, Sandra Lagler, Georgios Kalamakis, Ulrike Lischetti, Denis Jabaudon, Randall J. Platt
Summary: A direct in vivo single-cell CRISPR screening method, AAV-Perturb-seq, was used to dissect the phenotypic landscape of 22q11.2 deletion syndrome associated genes in the adult mouse brain prefrontal cortex. The study identified three 22q11.2-linked genes involved in neuronal functions, explaining approximately 40% of the observed transcriptional changes in a 22q11.2-deletion mouse model.
Article
Multidisciplinary Sciences
Kazuhiko Sawada, Shigeyoshi Saito
Summary: This study quantitatively characterized cerebellar asymmetry and sexual dimorphism in cynomolgus macaques using magnetic resonance (MR) imaging-based volumetry. The results showed significant left-lateralization in males in the central zone posterior to the cerebellar transverse domains, while no left/right bias was found in females. Additionally, population-level leftward asymmetry was revealed in the crus II region in males.
Article
Geriatrics & Gerontology
Kathryn L. L. Kern, Stephanie A. A. McMains, Thomas W. W. Storer, Scott D. D. Moffat, Karin Schon
Summary: Spatial navigation is critical for daily goal-directed behavior, but older adults experience decline in navigation performance. This study investigates the role of cardiorespiratory fitness in modulating brain activity during navigation in cognitively healthy older adults.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Qinglan Ling, Jessica A. Herstine, Allison Bradbury, Steven J. Gray
Summary: Recent advancements in gene supplementation therapy using adeno-associated virus (AAV) vectors for neurological disorders are discussed in this review. The authors highlight ongoing clinical trials and factors that impact the safety and efficacy of treatments. They also address unmet needs in the field and potential strategies to optimize AAV vector delivery and reduce potential toxicity.
NATURE REVIEWS DRUG DISCOVERY
(2023)
Article
Multidisciplinary Sciences
Guanghan Meng, Jian Zhong, Qinrong Zhang, Justin S. J. Wong, Jianglai Wu, Kevin K. Tsia, Na Ji
Summary: Understanding blood flow dynamics is crucial for studying the function of blood vessels. Current methods lack the resolution to monitor blood flow at a cellular level in large vessels. However, using a fast line-scanning module, researchers were able to achieve high-resolution imaging of blood flow in the cortex of awake mice. This breakthrough allowed them to measure cerebral blood flow and observe pulsatile blood flow patterns.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Clinical Neurology
Chie Morimoto, Akiko Uematsu, Hironori Nakatani, Yosuke Takano, Norichika Iwashiro, Osamu Abe, Hidenori Yamasue, Kiyoto Kasai, Shinsuke Koike
Summary: The study suggests that the morphology of Crus I/II plays a critical role in schizophrenia, with associations found between different genders and disease stages. Additionally, alterations in WM volume of Crus I/II may serve as a potential biological marker for early detection and treatment of individuals with UHR.
PSYCHIATRY AND CLINICAL NEUROSCIENCES
(2021)
Article
Biochemical Research Methods
Brenton T. Laing, Justin N. Siemian, Sarah Sarsfield, Yeka Aponte
Summary: Imaging neuronal activity in awake, behaving animals has rapidly enhanced our understanding of how the brain works, using in vivo microendoscopic imaging to see inside the brains of experimental animals, combining cutting edge targeting strategies and sophisticated analysis tools to identify neuronal activity patterns underlying changes in behavior and physiology. New users may find it challenging to understand the techniques and leverage this technology to best suit their needs.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Review
Ophthalmology
Grazyna Palczewska, Maciej Wojtkowski, Krzysztof Palczewski
Summary: The eye is a suitable organ for multi-photon excitation imaging due to the transparency of ocular tissues to infrared light. The interface between the retina and the retinal pigment epithelium (RPE) provides valuable information about the visual cycle and its response to various factors. Vitamin A-derived retinoids and bisretinoids can be detected using two-photon ophthalmoscopy, enabling functional studies of the retina. Recent advancements in safety and data interpretation have allowed for the first studies with humans.
PROGRESS IN RETINAL AND EYE RESEARCH
(2023)
Article
Cell Biology
Huan Xiong, Fei Tang, Yujie Guo, Ruxiang Xu, Peng Lei
Summary: Neural circuits are crucial for healthy brain function and disruptions can lead to diseases. Advanced microscopic techniques, such as two-photon laser scanning microscopy, have been used to study neural circuit changes during diseases. This review focuses on the dynamic rewiring of dendritic spines and modulation of calcium transients in the intact living brain, discussing their potential roles in neurological disorders like Alzheimer's disease, stroke, and epilepsy. Understanding these changes is important for future clinical interventions.
AGEING RESEARCH REVIEWS
(2023)
Article
Chemistry, Analytical
Beatriz Lozano-Torres, Juan F. Blandez, Irene Galiana, Jose A. Lopez-Dominguez, Miguel Rovira, Marta Paez-Ribes, Estela Gonzalez-Gualda, Daniel Munoz-Espin, Manuel Serrano, Felix Sancenon, Ramon Martinez-Manez
Summary: Advances in the detection of cellular senescence are crucial for monitoring age-related diseases, and the use of the HeckGal probe allows reliable detection of senescence both in vitro and in vivo. This technology provides a potential tool for detecting senescence in aged or damaged tissues.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Yi Chen
Summary: This review highlights the use of A beta-specific fluorescent probes for two-photon imaging of A beta plaques in vivo. The synthesis and detection mechanism of these probes, as well as their application in imaging A beta plaques in vivo, are discussed.
Article
Psychiatry
Paola Colucci, Enrico Marchetta, Giulia Federica Mancini, Phoebe Alva, Flavia Chiarotti, Mazahir T. Hasan, Patrizia Campolongo
TRANSLATIONAL PSYCHIATRY
(2020)
Article
Neurosciences
Neil Dalphin, Kevin Dorgans, Eugene Khaskin, Bernd Kuhn
Article
Neurosciences
M. Mar Reus-Garcia, Raudel Sanchez-Campusano, Julia Ledderose, Godwin K. Dogbevia, Mario Trevino, Mazahir T. Hasan, Agnes Gruart, Jose M. Delgado-Garcia
Summary: The claustrum (CL) is believed to be involved in sensorimotor integration and cognitive processes. Through recordings in rabbits during classical eyeblink conditioning, CL neurons were found to be significantly modulated during early sessions of CS/US presentations but not in well-trained animals. The CL appears to play a crucial role in the acquisition of associative learning, particularly in relation to the novelty of CS/US associations.
Article
Biochemistry & Molecular Biology
Sigita Augustinaite, Bernd Kuhn
Review
Physiology
Bernd Kuhn, Federico Picollo, Valentina Carabelli, Giorgio Rispoli
Summary: To understand the workings of the nervous system, it is essential to develop advanced techniques for real-time recording of neuronal electrical activity and to study how higher functions emerge from coordinated activity. Techniques such as patch pipettes and biocompatible probes allow for recording from single neurons and multiple neurons simultaneously, providing valuable insights into neuronal activity and function. Advanced methods like voltage imaging with fast voltage-sensitive dyes and two-photon microscopy offer high spatial resolution for studying voltage oscillations in the brain and dendritic voltage changes in awake animals.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shinobu Nomura, Ludovic Tricoire, Ivan Cohen, Bernd Kuhn, Bertrand Lambolez, Regine Hepp
Article
Biochemical Research Methods
Philipp Flotho, David Thinnes, Bernd Kuhn, Christopher J. Roome, Jonas F. Vibell, Daniel J. Strauss
Summary: The proposed variational method for the alignment and displacement estimation of 1D signals offers state-of-the-art results in terms of alignment quality and computing speeds, making it suitable for cases where high accuracy and speed are needed.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Neurosciences
Claudia Cecchetto, Stefano Vassanelli, Bernd Kuhn
Summary: The study introduces a novel multimodal technique combining two-photon imaging and multi-channel field potential recordings to investigate the spatiotemporal dynamics of neuronal signals in different layers of the barrel cortex in mice.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Biochemical Research Methods
Philipp Flotho, Shinobu Nomura, Bernd Kuhn, Daniel J. Strauss
Summary: This study presents a new motion compensation method called Flow-Registration, which is able to align and reconstruct 2-photon imaging data. The method outperforms previous alignment tools and is suitable for low signal-to-noise ratio data and high-divergence displacements during local drug injections.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Behavioral Sciences
Ray X. Lee, Greg J. Stephens, Bernd Kuhn
Summary: This study investigated the gradual emergence of stress reactions in mice through detailed behavioral analysis, finding that the observed behavioral changes were a result of stress incubation and required social bonding. Morphological changes in specific regions of the cortex also supported this finding. The non-invasive analytical methods used in this study may have applications beyond laboratory animals.
FRONTIERS IN BEHAVIORAL NEUROSCIENCE
(2022)
Article
Psychiatry
Mohieldin Youssef, Hiro Taiyo Hamada, Esther Suk King Lai, Yuji Kiyama, Mohamed El-Tabbal, Hiroshi Kiyonari, Kohei Nakano, Bernd Kuhn, Tadashi Yamamoto
Summary: Stress affects behavior and involves critical dynamic changes at multiple levels, including molecular pathways, neural circuits, and behavior. This study investigates the role of TOB in psychological stress machinery, revealing its impact at the molecular, neural circuit, and behavioral levels.
TRANSLATIONAL PSYCHIATRY
(2022)
Article
Chemistry, Multidisciplinary
Torsten Fischer, Bernd Kuhn, Xiuru Fan, Markus Benjamin Wilms
Summary: This study developed a customized steel based on HiperFer for additive manufacturing, which demonstrated great performance potential through in-build heat treatment and tailored microstructure to counteract manufacturing flaws. The additive manufacturing process also enabled the reduction of grain size, leading to increased plastic deformation.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
David Keller, Tamas Lang, Melinda Cservenak, Gina Puska, Janos Barna, Veronika Csillag, Imre Farkas, Dora Zelena, Fanni Dora, Stephanie Kuppers, Lara Barteczko, Ted B. Usdin, Miklos Palkovits, Mazahir T. Hasan, Valery Grinevich, Arpad Dobolyi
Summary: This study discovered a neuronal pathway from PIL to MPOA that plays an important role in the control of social grooming. The activation of PIL and MPOA neurons by physical contact and chemogenetic stimulation indicates their involvement in social grooming. Additionally, the activation of the PIL-MPOA pathway and the expression of the neuropeptide PTH2 were found to facilitate social grooming.
Article
Multidisciplinary Sciences
Leonidas Georgiou, Anai Echeverria, Achilleas Georgiou, Bernd Kuhn
Summary: This study reveals the relationship between localized Ca2+ microdomain activity in astrocytes and behavior and neuronal activity. By transferring genetically encoded Ca2+ indicators to astrocytes, the researchers found that locomotion extensively increased the frequency and size of Ca2+ signals, while sensory stimulation had only a subtle effect. The overlay of these signals resulted in behavior-dependent maps with characteristic Ca2+ activity hotspots, possibly representing memory engrams.
Article
Biochemical Research Methods
Sigita Augustinaite, Bernd Kuhn
Summary: ISI is a technique used to map the functional architecture of the cortex based on hemodynamic responses. The protocol described involves using a cranial window with an access port to identify the area of V1 in a head-fixed mouse, followed by targeted viral vector injection for subsequent two-photon imaging of V1 layer 6 corticothalamic neurons. For more details, refer to the paper by Augustinaite and Kuhn (2020b).