Article
Biology
Ivona Mateska, Anke Witt, Eman Hagag, Anupam Sinha, Canelif Yilmaz, Evangelia Thanou, Na Sun, Ourania Kolliniati, Maria Patschin, Heba Abdelmegeed, Holger Henneicke, Waldemar Kanczkowski, Ben Wielockx, Christos Tsatsanis, Andreas Dahl, Axel Karl Walch, Ka Wan Li, Mirko Peitzsch, Triantafyllos Chavakis, Vasileia Ismini Alexaki
Summary: In this study, the researchers found that IL-1β reduced SDHB expression by upregulating DNMT1 and methylation of the SDHB promoter, disrupting the TCA cycle and leading to succinate accumulation and disturbed steroidogenesis. These findings provide a mechanistic explanation for adrenal dysfunction in severe inflammation and offer a potential target for therapeutic intervention.
Article
Endocrinology & Metabolism
Benjamin M. Dungar, Chad D. Schupbach, Jessie R. Jacobson, Phillip G. Kopf
Summary: The study found that BDE-47 significantly increases adrenal corticosteroid secretion and upregulates gene expression of enzymes involved in corticosteroid synthesis and mitochondrial metabolism. In the rat experiment, adrenal weight and plasma corticosterone levels were significantly increased in the BDE-47 treatment groups, suggesting a potential disruption in corticosteroid regulation and supporting the role of PBDEs as endocrine disruptors.
Article
Endocrinology & Metabolism
Benjamin M. Dungar, Chad D. Schupbach, Jessie R. Jacobson, Phillip G. Kopf
Summary: The study found that BDE-47 significantly increased aldosterone and cortisol secretion in HAC15 cells, as well as gene expression related to corticosteroid synthesis and mitochondrial metabolism. In Sprague Dawley rats, adrenal weight was significantly increased in BDE-47 treatment groups, with a notable rise in plasma corticosterone levels in the 100 μg BDE-47/kg treatment group. These results indicate that BDE-47 disrupts corticosteroid secretion and supports the notion that PBDEs could act as endocrine disruptors.
Article
Biochemistry & Molecular Biology
Agata Fabiszewska, Magdalena Paplinska-Goryca, Paulina Misiukiewicz-Stepien, Malgorzata Woloszynowska, Dorota Nowak, Bartlomiej Zieniuk
Summary: Yarrowia lipolytica yeast is a model species capable of accumulating intracellular lipids. The synthesis of lipids can follow either a de novo pathway or an ex novo pathway. The ex novo pathway is influenced by the limitation of nitrogen source in the medium. It was also found that lipid synthesis in lipid-rich medium mainly occurs via the ex novo pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Jeffrey C. Francis, Jennifer R. Gardiner, Yoan Renaud, Ritika Chauhan, Yacob Weinstein, Celso Gomez-Sanchez, Anne-Marie Lefrancois-Martinez, Jerome Bertherat, Pierre Val, Amanda Swain
Summary: This study found that high expression of HOXB9 and other HOX factors is associated with poorer prognosis in ACC patients. HOXB9 can promote adrenal tumor progression in a sex-dependent manner, and tumor cells are dependent on HOX function for survival. These findings have identified HOX factors as potential drug targets for novel therapeutic approaches in ACC.
BRITISH JOURNAL OF CANCER
(2021)
Review
Endocrinology & Metabolism
Martina Tetti, Siyuan Gong, Franco Veglio, Martin Reincke, Tracy Ann Williams
Summary: Primary aldosteronism is the most common surgically curable form of hypertension. Recent advances in our understanding of the disease have focused on the underlying genetic variants that cause excess aldosterone production. The mechanisms of increased adrenal cortex mass are still not well understood, but the application of transcriptomics, metabolomics, and epigenetics has provided valuable insights.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wenke Jonas, Oliver Kluth, Anett Helms, Sarah Voss, Markus Jahnert, Pascal Gottmann, Thilo Speckmann, Birgit Knebel, Alexandra Chadt, Hadi Al-Hasani, Annette Schuermann, Heike Vogel
Summary: Current attempts to prevent and manage type 2 diabetes have had moderate effectiveness. A recent study identified the S100z gene as a potential causal gene for the diabetes QTL Nidd13/NZO, affecting beta-cell proliferation and apoptosis. This discovery provides new insights into the molecular mechanisms of diabetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Tarun Kumar, Jitendra S. Nigam, Shambhwi Sharma, Madhu Kumari, Jagjit Pandey
Summary: Adrenocortical carcinoma is a rare tumor with poor prognosis, commonly metastasizing to the lung, liver, and lymph nodes, while bone metastases are less frequent. This case report describes a 35-year-old male with ACC metastasizing to an uncommon site, the mandible ramus.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2021)
Article
Endocrinology & Metabolism
Ye Lynn Ko, Vaishnavi Kumar, Juliane Lippert, Salvador Diaz-Cano, Kassiani Skordilis, Otilia Kimpel, Stefan Kircher, Miriam Asia, Yasir S. Elhassan, Barbara Altieri, Cristina L. Ronchi
Summary: This article reports three cases where sporadic ACC was identified in patients with coexistent or previous history of melanoma. The researchers found a higher incidence of ACC in patients with melanoma.
BMC ENDOCRINE DISORDERS
(2023)
Article
Urology & Nephrology
Benjamin V. Stone, Jacob E. Tallman, Kelvin A. Moses
Summary: The study found that treatment of adrenocortical carcinoma at an academic center is associated with improved overall survival compared to community programs. Academic centers had more aggressive surgical treatment practices, and the survival benefit persisted even after controlling for treatment modality in multivariable analysis.
JOURNAL OF UROLOGY
(2021)
Article
Surgery
Sean M. Wrenn, Alessandra L. Moore, Hina J. Shah, Justine A. Barletta, Anand Vaidya, Kerry L. Kilbridge, Gerard M. Doherty, Heather A. Jacene, Matthew A. Nehs
Summary: This study focused on adrenocortical carcinoma (ACC) and found that higher maximum standard uptake value (SUVmax) on FDG-PET scan was associated with worse survival. The study concluded that FDG-PET may provide clinically useful information for prognosis and treatment.
AMERICAN JOURNAL OF SURGERY
(2023)
Article
Endocrinology & Metabolism
Kazuki Ishiwata, Sawako Suzuki, Katsushi Igarashi, Yutaro Ruike, Kumiko Naito, Akiko Ishida, Hanna Deguchi-Horiuchi, Masanori Fujimoto, Hisashi Koide, Yusuke Imamura, Shinichi Sakamoto, Tomohiko Ichikawa, Jun-ichiro Ikeda, Koutaro Yokote
Summary: In this study, clinicopathological and genetic analyses were performed on 30 patients with unilateral cortisol-producing adrenal tumors to investigate FDG-PET positive adrenal adenomas. The results showed high FDG accumulation in all adrenocortical carcinomas and 65% of benign adrenocortical adenomas. Adrenal adenomas with high FDG accumulation typically presented with distinct black appearance and specific characteristics.
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2021)
Article
Pathology
Salam Ashour, Jordan P. Reynolds, Sanjay Mukhopadhyay, Jesse K. McKenney
Summary: SMARCA4-deficient undifferentiated tumors have distinct clinicopathologic features, may present in the adrenal glands, and show loss of nuclear SMARCA4 expression, among other characteristic findings.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Cell Biology
Mingyue Li, Miao Bai, Yulun Wu, Shuo Yang, Lihua Zheng, Luguo Sun, Chunlei Yu, Yanxin Huang
Summary: Gastric adenocarcinoma (GAC) is a highly lethal malignant tumor with unclear pathogenesis. This study analyzed the gene expression profiles of GAC tumor tissues and adjacent normal tissues, identifying 465 differentially expressed genes (DEGs). Further analysis revealed enrichment of DEGs in pathways related to extracellular matrix, protein digestion and absorption, and gastric acid secretion. Six key genes associated with poor GAC prognosis were identified, including P4HA3 and MATN3, which have rarely been reported in relation to GAC. Functional analysis demonstrated that upregulation of P4HA3 enhanced the proliferation and migration ability of GAC cells. This study provides new insights for the selection of prognostic markers and therapeutic targets for GAC.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Cell Biology
Zhen Dong, Chunyi Li, Dawn Coates
Summary: The growing deer antler contains a stem cell niche that drives bone regeneration at a rapid rate. PTN is a highly expressed growth factor in the antler stem cell tissues, with potential interactions with receptors such as PTPRZ and ALK. In vitro studies show that PTN does not induce differentiation or proliferation in antler stem cells, but rather maintains their stem cell status.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dominikay Kazmierczak, Karol Jopek, Karolina Sterzynska, Michal Nowicki, Marcin Rucinski, Radoslaw Januchowski
Summary: This study aimed to detect changes in miRNA expression levels in two cisplatin and two paclitaxel-resistant variants of the A2780 ovarian cancer cell line, and to identify miRNAs responsible for the regulation of drug-resistance genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Afsaneh Golkar-Narenji, Pawel Antosik, Shelly Nolin, Marcin Rucinski, Karol Jopek, Agnieszka Zok, Jaroslaw Sobolewski, Maurycy Jankowski, Maciej Zdun, Dorota Bukowska, Katarzyna Stefanska, Jedrzej M. Jaskowski, Hanna Piotrowska-Kempisty, Paul Mozdziak, Bartosz Kempisty
Summary: Modern science is committed to finding environmentally friendly solutions, and cellular agriculture is an important field in sustainable agriculture. This study compared different culture methods for myosatellite cells and found that suspension culture may cause the loss of physiological characteristics and gain of plasticity. Further investigation is needed to ensure the viability of suspension culture for industrial application.
Article
Biochemistry & Molecular Biology
Piotr Stasiak, Dominika Kazmierczak, Karol Jopek, Michal Nowicki, Marcin Rucinski, Radoslaw Januchowski
Summary: Epithelial ovarian cancer has the highest mortality due to late diagnosis and resistance to chemotherapy. MiRNA can regulate the expression of drug-resistance genes, possibly affecting the sensitivity of cancer cells to chemotherapy drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Marianna Tyczewska, Marta Szyszka, Karol Jopek, Marcin Rucinski
Summary: The expression changes of peptides and their receptors, as well as steroidogenesis enzymes, were investigated in the hypothalamus, pituitary, and adrenal glands of rats after intraperitoneal administration of peptides. The results showed significant expression changes in the hypothalamus and pituitary after 1-hour exposure to peptides, including a decrease in corticotropin-releasing hormone expression and a decrease in the expression of galanin receptors. The expression of steroidogenesis enzymes also changed, with an increase in cholesterol desmolase expression after peptide administration.
ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Stefanska, Lucie Nemcova, Malgorzata Blatkiewicz, Wojciech Pienkowski, Marcin Rucinski, Maciej Zabel, Paul Mozdziak, Marzenna Podhorska-Okolow, Piotr Dziegiel, Bartosz Kempisty
Summary: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) possess multilineage differentiation potential and specific surface markers. In this study, RNA sequencing analysis revealed differentially expressed genes associated with apoptosis and identified upregulation of ZBTB16 and FOXO1, as well as downregulation of TGFA, in all differentiated groups. Additionally, several novel marker genes related to WJ-MSCs differentiation were identified. This study sheds light on the molecular mechanisms involved in long-term culture and differentiation of WJ-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marianna Tyczewska, Patrycja Sujka-Kordowska, Marta Szyszka, Karol Jopek, Malgorzata Blatkiewicz, Ludwik K. Malendowicz, Marcin Rucinski
Summary: The homeostasis of the adrenal gland is crucial for its proper functioning in both non-stressful and stressful conditions, involving interactions between different types of cells. However, there is insufficient information regarding marker gene expression in the rat adrenal gland under non-stressful conditions. This study aimed to determine the expression of marker genes in different zones of the rat adrenal gland and revealed distinct expression patterns of interstitial cell markers in specific zones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Malgorzata Blatkiewicz, Kacper Kaminski, Marta Szyszka, Zaid Al-Shakarchi, Anna Olechnowicz, Ewelina Stelcer, Hanna Komarowska, Marianna Tyczewska, Anna Klimont, Marek Karczewski, Tomasz Wierzbicki, Joanna Mikolajczyk-Stecyna, Marek Ruchala, Ludwik K. Malendowicz, Marcin Rucinski
Summary: The study examines the association of ZWILCH gene with adrenocortical carcinoma (ACC). The higher expression of ZWILCH gene was found in ACC tissue compared to normal adrenal glands. Upregulation of ZWILCH is correlated with tumor mitotic rate and patient survival. The enhanced ZWILCH level is also associated with activation of genes involved in cell proliferation and inhibition of genes related to the immune system.
Article
Biochemistry & Molecular Biology
Remigiusz Domin, Michal Pytka, Mikolaj Zolynski, Jan Nizinski, Marcin Rucinski, Przemyslaw Guzik, Jacek Zielinski, Marek Ruchala
Summary: This study aimed to investigate the relationship between serum MOTS-c concentration and muscle strength parameters, as well as oxygen consumption, in physically active individuals. The results showed that higher serum MOTS-c concentration was associated with greater muscle mass, force, and power, but not with exercise capacity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Natalia Izycka, Marcin Rucinski, Malgorzata Andrzejewska, Sebastian Szubert, Ewa Nowak-Markwitz, Karolina Sterzynska
Summary: Recurrent disease and treatment-associated chemoresistance are major contributors to poor clinical outcomes in ovarian cancer patients. This study investigated the expression of CSC-associated markers ALDH1A, CD44, and CD133 to predict patient prognosis. ALDH1A1-positive patients had significantly shorter overall survival (OS) and progression-free survival (PFS). Multivariate analysis revealed that ALDH1A1 expression, FIGO stage III-IV, and residual disease were associated with shorter OS. Targeting ALDH1A1-positive cancer cells may be a promising therapeutic strategy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Agnieszka Anna Rawluszko-Wieczorek, Julia Lipowicz, Marta Nowacka, Kamila Ostrowska, Paulina Pietras, Malgorzata Blatkiewicz, Marcin Rucinski, Pawel Piotr Jagodzinski, Michal Nowicki
Summary: The presence of estrogen receptor beta (ER beta) is inversely correlated with the occurrence of colorectal cancer (CRC), and low expression of ER beta is associated with poorer overall survival of CRC patients. ER beta can inhibit tumorigenesis through various molecular pathways and also has an impact on the hypoxic response in CRC cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)