Article
Chemistry, Medicinal
Michal Cesnek, Michal Safranek, Martin Dracinsky, Eva Tloust'ova, Helena Mertlikova-Kaiserova, Michael P. Hayes, Val J. Watts, Zlatko Janeba
Summary: A series of potent acyclic nucleoside phosphonates (ANPs) were designed as inhibitors of bacterial ACs, showing substantial selectivity and significant inhibitory effects in cell-based and cell-free assays. These compounds represent the most effective ACT/EF inhibitors based on ANPs reported to date.
Article
Food Science & Technology
Marilyne Davi, Mirko Sadi, Irene Pitard, Alexandre Chenal, Daniel Ladant
Summary: Various bacterial pathogens are producing toxins that target the cyclic Nucleotide Monophosphate (cNMPs) signaling pathways in order to facilitate host colonization. Here we describe a simple and robust in vitro assay for AC activity based on the spectrophotometric detection of cyclic AMP (cAMP) after chromatographic separation on aluminum oxide.
Article
Immunology
Mariela del Carmen Carrica, Juan Pablo Gorgojo, Yanina Andrea Lamberti, Hugo Alberto Valdez, Maria Eugenia Rodriguez
Summary: B. parapertussis CyaA intoxicates human macrophages, prevents bacterial phagocytosis, and hinders phago-lysosomal fusion, promoting bacterial survival. CyaA also causes downregulation of host genes involved in antimicrobial defense and pro-inflammatory response, while upregulating anti-inflammatory cytokine IL-10. CyaA plays a central role in immune evasion and persistence of B. parapertussis.
MICROBIAL PATHOGENESIS
(2023)
Article
Chemistry, Medicinal
Petra Brehova, Ema Chaloupecka, Michal Cesnek, Jan Skacel, Martin Dracinsky, Eva Tloustova, Helena Mertlikova-Kaiserova, Monica P. Soto-Velasquez, Val J. Watts, Zlatko Janeba
Summary: A series of novel acyclic nucleoside phosphonates (ANPs) were synthesized and evaluated as potential adenylate cyclase inhibitors, showing promising inhibitory effects on various toxins and enzymes. Some compounds were identified as potential leads for the treatment of human inflammatory and neuropathic pain.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Pavel Kraina, Michal Cesnek, Eva Tloustova, Helena Mertlikova-Kaiserova, Camryn J. Fulton, Emily K. Davidson, Brenton P. Smith, Val J. Watts, Zlatko Janeba
Summary: A series of novel 7-substituted 7-deazaadefovir analogues were synthesized, and 12 of them were found to be single-digit micromolar inhibitors of Bordetella pertussis adenylate cyclase toxin. Compound 14 was identified as a potent, non-toxic, and selective mAC2 inhibitor.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Engineering, Chemical
Dahai Jiang, Zhidi Min, Jing Leng, Huanqing Niu, Yong Chen, Dong Liu, Chenjie Zhu, Ming Li, Wei Zhuang, Hanjie Ying
Summary: In this study, two novel class III ACs with halophilic property were discovered from Thermobifida halotolerans DSM 44931 (ThAC) and Haloactinopolyspora alba DSM 45211 (HaAC), respectively. The presence of NaCl significantly enhanced the enzyme activities of ThAC and HaAC, and several divalent metal ions were found to activate the recombinant ACs to different extents. This is the first report of halophilic Class III ACs, which is important for further investigations.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Engineering, Electrical & Electronic
Yuchen Zhang, Simone Buso, Tommaso Caldognetto
Summary: This article presents the topology and control of a three-port energy gateway (EG) for hybrid ac/dc nanogrids. The proposed EG represents a tradeoff between circuit complexity and controls flexibility, allowing for a wide range of operation modes and power flow.
IEEE JOURNAL OF EMERGING AND SELECTED TOPICS IN POWER ELECTRONICS
(2022)
Article
Biotechnology & Applied Microbiology
A. Regmi, J. G. Tague, K. E. Boas Lichty, E. F. Boyd
Summary: This study characterized the roles of CRP and CyaA in V. parahaemolyticus and found that cAMP-CRP is an activator of metabolism, motility, capsule production, and biofilm formation. This is in contrast to the function of cAMP-CRP in V. cholerae, which represses capsule and biofilm formation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mateusz Kwiatkowski, Aloysius Wong, Anna Kozakiewicz, Christoph Gehring, Krzysztof Jaworski
Summary: This study uncovered the elusive cyclic nucleotide phosphodiesterases (PDEs) in plants and identified a potential PDE, Arabidopsis thaliana K+-Uptake Permease (AtKUP5). The PDE activity was confirmed through molecular docking analysis and was found to be stimulated by the Ca2+/CaM complex.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Review
Chemistry, Multidisciplinary
Lili Tan, Wenlong Fu, Qi Gao, Peng-peng Wang
Summary: Chirality introduces new possibilities for materials by adding a new dimension of functionality, and when combined with plasmonic hybrid nanostructures, it creates unprecedented chiroptical materials with enhanced capabilities. Recent advancements in chiral plasmonic hybrid nanomaterials have allowed for the harnessing of their optical activity and hybrid components, leading to applications in sensing, catalysis, and optics. However, there is a need for a comprehensive synthesis of current knowledge and construction techniques in this field. This review provides an overview of the origins and synthesis of chiral plasmonic hybrid nanostructures, as well as their versatile applications, and discusses the challenges and future prospects of this advanced field.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Lina Wu, Liuqin Su, Minfang Deng, Xinyi Hong, Mingkai Wu, Miaomiao Zhang, Emmanuelle Bouveret, Xiaomei Yan
Summary: Quantitative measurement of protein-protein interactions (PPIs) remains challenging, but a new method using CRISPR/Cas9 technology to insert fluorescent protein tdTomato and 12-amino acid tetracysteine tag shows promise in simplifying this process. This method allows for rapid and high-throughput analysis of PPIs at the single-cell level and has been demonstrated to be effective in ranking binding affinities, mapping residues involved in PPI, and screening protein interaction inhibitors.
Article
Biochemistry & Molecular Biology
Masayo Omura, Yukie Takabatake, Eugene Lempert, Sigi Benjamin-Hong, Charlotte D'Hulst, Paul Feinstein
Summary: The molecular basis of odor perception in humans remains elusive due to the challenges in studying odorant receptors (ORs) outside their natural environment. This study presents a method using cilia isolated from mouse olfactory sensory neurons to measure odor-specific responses with higher sensitivity. This approach offers a sensitive and scalable platform for ex vivo odor screening.
Article
Microbiology
Yunfei Cai, Xue Chen, Peixuan Li, Weiheng Ren, Qiang Zhang, Yiwen Wang, Yina Jiang, Pinkuan Zhu, Hideyoshi Toyoda, Ling Xu
Summary: This study reveals that the conserved residue S1407 of BAC is a vital phosphorylation site that regulates the cAMP signaling pathway and affects the photomorphogenesis, circadian rhythm, and pathogenicity of B. cinerea.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Physiology
Rasoul Eslami, Abdolhossein Parnow, Zahra Pairo, Pantelis Nikolaidis, Beat Knechtle
Summary: The study showed that both moderate-intensity and high-intensity aerobic exercise could reduce headache intensity, frequency, and duration in women with migraine, with moderate-intensity exercise being more effective in improving headache intensity and duration. However, neither exercise intensity had an effect on the levels of PACAP and SP in the participants.
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Gil Zimran, Erez Feuer, Oded Pri-Tal, Michal Shpilman, Assaf Mosquna
Summary: Developing sensory modules for specific molecules is a challenge in synthetic biology. This study demonstrates a generalizable approach by evolving a chemically induced heterodimer into a genetically encoded sensor for herbicides. The results provide a potential scaffold for the development of biosensors and lay down the groundwork for future research.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Food Science & Technology
Alexis Voegele, Mirko Sadi, Dorothee Raoux-Barbot, Thibaut Douche, Mariette Matondo, Daniel Ladant, Alexandre Chenal
Editorial Material
Immunology
Daniel Ladant, Carmen Buchrieser, Michel R. Popoff
PATHOGENS AND DISEASE
(2019)
Article
Multidisciplinary Sciences
Christelle Angely, Daniel Ladant, Emmanuelle Planus, Bruno Louis, Marcel Filoche, Alexandre Chenal, Daniel Isabey
Article
Gastroenterology & Hepatology
Christian Borup, Signe Wildt, Juri Rumessen, Jesper Graff, Pierre Nordine Bouchelouche, Trine Borup Andersen, Lars Vinter-Jensen, Anna Zaremba, Soren Peter German Jorgensen, Tine Gregersen, Camilla Nojgaard, Hans Bording Timm, Dominique Rainteau, Emilie Gauliard, Lars Kristian Munck
AMERICAN JOURNAL OF GASTROENTEROLOGY
(2020)
Article
Nutrition & Dietetics
Antonin Lamaziere, Dominique Rainteau, K. C. Pukar, Lydie Humbert, Emilie Gauliard, Farid Ichou, Maharajah Ponnaiah, Nadine Bouby, Joe-Elie Salem, Jean-Maurice Mallet, Maryse Guerin, Philippe Lesnik
Review
Food Science & Technology
Daniel Ladant
Summary: The adenylate cyclase toxin CyaA is a key virulent factor produced by Bordetella pertussis, targeting innate immunity to facilitate bacterial colonization of the respiratory tract. With remarkable characteristics, CyaA has been utilized for various applications in vaccinology and biotechnology. Its engineering as a vaccine vehicle for antigen delivery and as a genetic assay for monitoring protein-protein interactions in bacteria are briefly summarized in this chapter.
Article
Chemistry, Multidisciplinary
Alexis Voegele, Mirko Sadi, Darragh Patrick O'Brien, Pauline Gehan, Dorothee Raoux-Barbot, Maryline Davi, Sylviane Hoos, Sebastien Brule, Bertrand Raynal, Patrick Weber, Ariel Mechaly, Ahmed Haouz, Nicolas Rodriguez, Patrice Vachette, Dominique Durand, Sebastien Brier, Daniel Ladant, Alexandre Chenal
Summary: The P454 segment of the CyaA toxin is capable of translocating across membranes and interacting with calmodulin, with key residues involved in membrane destabilization and calmodulin binding. Inhibitors of calmodulin can efficiently block CyaA internalization. This suggests a potential mechanism for the vectorial translocation of the toxin into host cells.
Editorial Material
Food Science & Technology
Daniel Ladant, Pascale Marchot, Sylvie Diochot, Gilles Prevost, Michel R. Popoff, Evelyne Benoit
Summary: This report presents the 27th annual meeting of the French Society of Toxinology, which focused on the dual nature of toxins and showcased the latest research findings on plant, fungal, algal, animal, and bacterial toxins.
Article
Microbiology
Hazel Silistre, Dorothee Raoux-Barbot, Federica Mancinelli, Flora Sangouard, Alice Dupin, Alexander Belyy, Vincent Deruelle, Louis Renault, Daniel Ladant, Lhousseine Touqui, Undine Mechold
Summary: ExoY is an effector injected by Pseudomonas aeruginosa into host cells, with prevalence among strains and the potential to partly alleviate the cytotoxic effects of other virulence factors under certain conditions.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biology
Corentin Leger, Irene Pitard, Mirko Sadi, Nicolas Carvalho, Sebastien Brier, Ariel Mechaly, Dorothee Raoux-Barbot, Maryline Davi, Sylviane Hoos, Patrick Weber, Patrice Vachette, Dominique Durand, Ahmed Haouz, J. Inaki Guijarro, Daniel Ladant, Alexandre Chenal
Summary: We conducted a molecular characterization of CaM:CDZ complexes using SEC-SAXS, X-ray crystallography, HDX-MS, and NMR methods. The binding of CDZ causes conformational reorientation of CaM and stabilizes its structural elements, leading to reduced protein dynamics. Residues involved in the stabilization of the CaM:CDZ complex were identified. These findings provide insights into CDZ-induced dynamics and structural changes of CaM, which can be used for the design of more selective CaM antagonists.
Article
Food Science & Technology
Marilyne Davi, Mirko Sadi, Irene Pitard, Alexandre Chenal, Daniel Ladant
Summary: Various bacterial pathogens are producing toxins that target the cyclic Nucleotide Monophosphate (cNMPs) signaling pathways in order to facilitate host colonization. Here we describe a simple and robust in vitro assay for AC activity based on the spectrophotometric detection of cyclic AMP (cAMP) after chromatographic separation on aluminum oxide.
Article
Immunology
Zuzana Antosova, Nada Podzimkova, Jakub Tomala, Katerina Augustynkova, Katerina Sajnerova, Eva Nedvedova, Milada Sirova, Guy de Martynoff, David Bechard, Ulrich Moebius, Marek Kovar, Radek Spisek, Irena Adkins
Summary: SOT101, a potential clinical candidate for cancer treatment, activates NK cells and CD8(+) T cells and enhances their cytotoxicity against tumor cells. When used in combination with approved monoclonal antibodies, it increases the killing of tumor cells. In an animal model, the combination of SOT101 and Daratumumab showed the strongest anti-tumor effect, supporting further investigation of this combination in clinical studies.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Food Science & Technology
Daniel Ladant, Gilles Prevost, Michel R. Popoff, Evelyne Benoit
Meeting Abstract
Oncology
Irena Adkins, Zuzana Antosova, Klara Danova, Kamila Hladikova, Katerina Augustynkova, Katerina Sajnerova, Nada Podzimkova, Pavel Marasek, Guy de Martynoff, David Bechard, Ulrich Moebius, Radek Spisek
Meeting Abstract
Oncology
Zuzana Antosova, Nada Podzimkova, Marketa Jiratova, Eva Nedvedova, Guy De Martynoff, David Bechard, Ulrich Moebius, Radek Spisek, Irena Adkins
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2020)